Objective To study hepatotoxicity and superoxide dismutase(SOD) acitvity produced by mixture of Nickel Sulfate,Potassium Dichromate and Cobalt Chloride.Mehtods Hepatotoxicity and superoxide dismutase(SOD)activity of mixture of Nickel Sulfate,Potassium Dichromate and Cobalt Chloride were detected by ip in mice.Results The liver weights were increased consistently during three weeds treatment.It was showed in histology study that hepatocytes swelling,necrosis and congestion wigh blood in venae interlobares hepatis.The SOD activity of liver and blood decreased after one week treatment but increased the following two weeks treatment.Conclusions Mixture of Nickel Sulfate,Potassium Dichromate and Cobalt Chloride had obvious hepatotoxicity and a certain lipid peroxiodation effect in vivo.

Objective To study the adverse effect of amatoxins on DNA in the histiocytes of rats and the antagonism of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA). Methods SD rats were randomly divided into three groups, the number of males and females was the same. The rats in the experimental groups were respectively treated with amatoxins(0.1 mg/kg bw, i.p.) and amatoxins added EPA, DHA (both were 10 mg/kg bw, i.p.) for 10 days. Single cell gel-electrophoresis was employed to test the damage of DNA in the histiocytes. Results In the group treated with amatoxins, DNA damage in the peripheral lymphocyte, hepatocytes, lung cells ,brain cells was more seriously compared with the control, but no significant DNA damage was seen in the group treated with amatoxins added EPA, DHA. Conclusion Amatoxins may induce DNA damage and eicosapentaenoic acid, docosahexaenoic acid have an antagonism to the adverse effect of amatoxins.

Objective To determine the inhibitory effect of the synthetic Nrf 2 siRNA on the expression of Nrf2 gene in human laryngeal cancer cell lines Hep2 and to investigate the effects of Nrf2 siRNA on chemosensitivity of laryngeal carcinoma to cisplatin by detection growth and apoptosis in Hep2 cells .Methods The recombinant plas‐mid control siRNA and Nrf2 siRNA were transfected into Hep2 cells ,and western blot analysis of Nrf2 expression in Hep2 cells was performed 48 h after transfection .In order to determine whether Nrf2 siRNA can enhance the sensi‐tivity of Hep2 laryngeal cells to cisplatin ,we treated Hep2 cells with different concentrations of cisplatin after 24 h , and evaluated these cells for proliferation ,and apoptosis .CCK - 8 and flow cytometry assay were used for determi‐nation of cells proliferation and apoptosis in Hep2 cells .We calculated the inhibition rate and IC50 of the cell after treating with different concentrations of ciplatin .Results The laryngeal carcinoma cell stain Hep2 was transfected by Nrf2 siRNA and control siRNA respectively .The result of western blot showed the Nrf 2 expression was signifi‐cantly impeded at protein levels .CCK - 8 assay showed the proliferation of Hep2/Nrf2 siRNA and Hep2/ control siRNA was inhibited to 35 .55% to 46 .07% at 24 h respectively after treating with 4 μg/ml cisplatin .The chemo‐sensitivity to cisplatin in Hep2/Nrf2 siRNA was markly increased compared with Hep2/control siRNA .The IC50 in Hep2/Nrf2 siRNA was 5 .27 μg/ml contrast to 8 .107 μg /ml compared in Hep2/control siRNA .The result of flow cytometry analysis showed the apoptosis rate after Nrf 2 depletion was increased from 17 .1% to 26 .6% .Conclusion This study demonstrates that Nrf2 siRNA effectively inhibits Nrf2 gene expression in Hep2 cells leading to growth suppression and induction of apoptosis in Hep2 cells under cisplatin .The use of siRNA technique may pro‐vide a novel therapeutic approach to treat laryngeal cancer for enhance chemosensitivity .

Objective To investigate the epidemiologic characteristics of injuries of children aged 0 ~14 yrs. Methods Described analysis was used to analyze the epidemiologic characteristics about the age, gender, injury type and cause in children 0~14 years old who visited in emergency department of hospitals form 2006 to 2007. Results During the study period, 16 124 injured children were collected. the rank of injuries type were contused/abrased wound (44.41%), open injury (36.10%), sprain (12.81%), bone fracture (3.25%) and burn/scald injury (1.49%); the cause of injuries were fall down (55.24%), animal injury (21.49%) , blunt injury (8.47%) and traffic injury (6.96%); The common place of injury occurred were at home (53.66%), public and resident place (18.01%), road/street (11.73%) and school (10.70%). The gender ratio was 1.94, the median age was 6 years, the male injury was decrease with the age increase, but so wasn't the female. Conclusion Injury gradually becomes the key health problem and influences the children health. The effective measures should be taken in term of the epidemiology characteristics of the injuries among children at 0-14 to prevent and control injury occurring. The health education of preventing injury occurring and behavior drawback should be conducted as early as possible to raise awareness of injury issues among children in the kindergartens and promote them growing healthily.

Objective To observe the effect of propofol target-controlled infusion on stress response during nasoscopic procedures.Methods Totally 40 patients with ASA gradesⅠ-Ⅱ scheduled for the nasoscopic operation ware randomly divided into two groups:Group A(propofol continuously injection,2.5 mg?kg-1,n=20)and Group B(propofol target-controlled infusion,4 ?g?ml-1,n=20).The operations were all performed under general anesthesia.Venous blood samples were taken to measure cortisol and blood glucose at three time points:before operation,at 30 min after the operation started,and 60 min after the endotracheal catheter was withdrawn.Meanwhile,HR and MAP of the patients were recorded.Results At both 30 min after the operation started and and 60 min after the endotracheal catheter was withdrawn,Group A showed significantly higher MAP and serum levels of glucose and cortisol than Group B.At 30 min after the operation started:HR:(73?8)/min vs(65?13)/min,t=2.344,P=0.024;MAP:(74?7)mm Hg vs(68?7)mm Hg,t=2.711,P=0.010;blood glucose:(6.28?0.11)mmol/ml vs(5.31?0.15)mmol/ml,t=23.321,P=0.000;cortisol:(125.3?11.5)ng/ml vs(89.6?9.9)ng/ml,t=10.521,P=0.000.At 60 min after the endotracheal catheter was withdrawn:MAP:(79?6)mm Hg vs(73?8)mm Hg,t=2.683,P=0.011;blood glucose:(6.18?0.09)mmol/ml vs(5.62?0.16)mmol/ml,t=10.082,P=0.000;cortisol:(169.1?16.3)ng/ml vs(149.5?15.3)ng/ml,t=3.921,P=0.000.Conclusion Propofol target-controlled infusion can inhibit the stress response caused by nasoscopic operation.

Objective To observe the change of composition of PL and content of protein in PS after anatoxin damnify lung.Methods The rats lung injury models were made by intratracheally instilling anatoxin(0.1mg/kg,0.4mg/kg).24 rats were divided into four groups:12hour group,2day group,3day group,5day group.Pr of each group were examined by electron microscope,content of PL,composition of PL and content of protein of each group wete determinde respectively.Results Rats lungs in experimental guoups were found that PS lost continuously,dropped in the pulmonary alveolies.12hour group was more apparent.Content of protein in PS was the highest in 12hour group,Content of PL in PS go add,12hour group was more apparent.Conclusion Morphologic change and alternation of quality and quantity of PS after anatoxin-induced pulmonary injures specifically reflect the activity of lung danmify.Measuring content of PL and PI is sign of simple and feasible method when lungs of the rats are injured early by bleomycin.

To find immunomorphologic evidence of interregulation between neuroendocrine system and immune system by detecting 5-HT in human normal lymph-node and lymphoma.Methods:Immunohistochemical SABC method and statistics analysis.Results:Thirty-three cases of lymph-nodes showd 5-HT positive expression.The positive rate of lymphoma is lower than that of normal lymph-node.The differences between human normal lymph-nodes and lymphoma are significant( P

Objective To investigate the clinical application of laparoscopic adrenalectomy and summarize the experience in laparoscopie adrenalectomy.Methods From August 2002 to March 2007,21 cases of benign adrenal tumors were treated with retroperitoneal laparoscopy in this hospital.There were 9 cases of adrenocortical adenoma, 7 cases of primary aldosteronism,3 cases of adenocorticol macronodular hyperplasia,1 case of pheochromccytoma,and 1 case of adrenal gangliocytoma.Results Retroperitoneal laparoscopy was successfully applied in 21 cases.Operating time was between 65 and 130min with an average of 95 min.All patients did not receive blood transfusion and had no obvious complications.Conclusion Laparoscopic adrenalectomy had the advantages of minimal morbidity,mini- mal postoperative discomfort and a short hospital stay,whieh had a good prospect for application in the clinical prac- tice.

Objective To study the effects of mosapride and domperidone on the pulmonary infection of acute stroke patients lying in bed and with nasal feeding. Methods Eighty-nine acute stroke patients lying in bed and with nasal feeding were divided randomly into the treatment group (47 cases) and the control group (42 cases). The control group was treated routinely,and the treatment group was treated with mosapride 5 mg and domporidone 20 mg thrice a day for 4 weeks, besides routine therapy. The incidence rate of pulmonary infection, gastric residual volume (GRV) and the number of cases with gastric contents remaining after 3 hours of nasal feeding were studied. All data were analyzed statistically. Results In the treatment group, 13 cases had pulmonary infection,and the incidence rate was 27.66%(13/47). In the control group,25 cases had pulmonary infection,and the incidence rate was 59.52% (25/42). There was significant difference between the two groups (P < 0.01 ). Three hours after nasal feeding,24 cases with gastric contents remaining were discovered in the treatment group,and GRV was (50.80±15.38) ml. Two hundred and thirty-seven cases with gastric contents remaining were discovered in the control group, and GRV was (112.17±32.54) ml. Significance differences were also detected between the two groups (P<0.01). Conclusion As for the acute stroke patients lying in bed and with nasal feeding,mosapride and domperidone can remarkably cut down the pulmonary infection upon common treatment.

Objective To observe the changes of S-opsin expression in guinea pigs with flickering light-induced and form-deprived myopia,and to investigate the causes.Methods Thirty-six two-week-old healthy guinea pigs were randomly assigned to three groups:Flickering light-induced myopia group(FLM group,n=13),form-deprived myopia group(FDM group,n=12) and control group(n=11).For the FLM group,the cages were equipped with astroboscope(0.5 Hz),and LEDs were used as the light source.The right eyes of the guinea pigs in FDM group wore translucent goggles which did not interfere with the normal activity of their eyelids.No special treatment was given to the guinea pigs in the normal groups.All measurements were performed prior to and then after 6 weeks of treatment.The first measurement day was recorded as 0 week.Biological parameters,such as the refraction,axial length(AL) and corneal radius of curvature(CRC),were measured and fundus photography is performed before and after 6 weeks of the treatment.The expression of S-opsin was observed and analyzed by immunofluorescence technique and image analysis system.Results Before the treatment,no significant difference was found in three biometric measurements including refraction,AL and CRC between the groups at 0 week(P>0.05).After the treatment for 6 weeks,significant differences were found in changes of both the biometric measurements between the FLM and control groups,and between the FDM and control groups(P<0.05).However,no significant differences were found in the changes of CRC among the FLM,FDM and control groups(P=0.358),indicating that myopia models were established successfully.No significant differences were found in the changes of values of refraction,AL and CRC between the FLM and FDM groups(P>0.05).Expression of S-opsin differed in the FLM and FDM groups.For the mean gray values of green channel,compared with the control group respectively,significant differences were found in both the FLM and FDM group(P<0.001).The mean gray value of green channel of the FLM group was higher,however the mean gray value of green channel of the FDM group was lower.Conclusions Both guinea pig models of flickering light-induced and form-deprived myopia can be established successfully.S-opsin is increased in the flickering light-induced myopia model and decreased in the form-deprived myopia model,indicating that the mechanisms of formation of these two experimental myopia models may be different.