Objective: To investigate the CE fingerprint of Salvia miltiorrhiza . Methods : Separation was performed on a 50?m?50cm uncoated capillary with 20mmol?L -1 borate solution as CE buffer. The run voltage was 15kV and the UV detection was set at 210nm. Results : Fingerprint consisted of 11 common peaks. The validation of methods meet the requirements for SDA's technical regulations. Conclusion : The method was accurate and simple for quality control of Salvia miltiorrhiza.

Through the analysis on different development paths of western and Chinese medical ethics, this article proposed to construct the Chinese medical ethical review system. Although the medical ethical review system was originated in western countries, due to different origins of medical ethical thoughts, different cultures and beliefs among races, as well as different medical and science development models, the application of western medical eth-ical review system should not be mechanically used in China. The application should follow the idea of har-monization with differences. This article discussed on current status and significance of the construction of an ethical review platform which is suitable to the national conditions of China. It was also emphasized on the im-portance of ethics training among researchers.

Adolescent ; Adult ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Medicine, Chinese Traditional ; methods ; Ovarian Diseases ; physiopathology ; therapy ; Ovary ; physiopathology ; Phytotherapy ; Young Adult

Objective To investigate Bcl-2 expression in the peripheral blood mononuclear cells and its clinical significance of liver fibrosis (LF). Methods Tested Bcl-2 protein levels in T, B cells of 47 patients (male 17, female 30) with LF, 35 patients (male 24, female 11) with chronic hepatitis B and no LF and 41 cases (male 29, female 12) normal controls by two color cytofluorography. Results Among them, LF patients, chronic hepatitis B without LF and normal controls, the proposition of T cells (including CD3~+, CD4~+ and CD8~+ subgroups) and CD19~+ B cells expressed Bcl-2 protein increased significantly in LF patients (P

ObjectiveTo evaluate the feasibility of detecting the mutations of KRAS,EGFR in nonsmall-cell lung cancer and colorectal cancer patients with high resolution melting curve analysis. MethodsAt first,the mutations in KRAS exon 2 and EGFR exons 18 to 21 of 5 patients with non-small cell lung cancer and 5 patients with colorectal cancer were detected by high resolution melting curve analysis.Then the results were confirmed with direct sequencing. ResultsBy high resolution melting curve analysis,1 of 10 patients was found to carry EGFR 19 mutation who was harboring 2236-2250del mutation.By direct sequencing,consistent results of the patients with mutations orwithout mutations were got. Conclusion s The new high resolution melting curve analysis was more efficient,more convenient than direct sequencing.Moreover,it was a low-cost test,which was suitable for clinic test.

Objective To study diagnosis significance of neutrophil surface adhesion molecules including CD11b,in type 2 diabetes mellitus patients with cytomegalovirus (CMV)infection.Methods Blood samples from 139 cases of type 2 diabetes mellitus patients were collected including 99 male and 40 female cases between Septem-ber 2011 and January 2015.Healthy male 20 cases and female 13 cases were collected as healthy control group from outpatients in the same period.Type 2 diabetes mellitus patients were divided into high active CMV infection group, CMV infection group and the negative control group,and healthy group as normal control group according to CMV -pp65 antigen detection.Neutrophil surface adhesion molecule CD11b was analyzed by flow cytometry and compared respectively.ROC curve of CD11b in 2 diabetes mellitus patients was made.Results The expression ratio of CD11b in high active CMV infection group and CMV infection group in type 2 diabetes mellitus patients was (41.25 ± 5.33)%.There was significant difference (P =0.019)compared with negative control group (61.13 ±5.28)% and there was significant difference (P =0.009)compared with negative control group (64.21 ±6.02)%;optimal cut -off value of CD11b in high active CMV infection group was 45.65%,the sensitivity was 100.00%,the specificity in high active CMV infection group was 60.12%,and the area under the receiver -operating curve(ROC)was 0.628;optimal cut -off value of CD11b in CMV infection group was 70.21%,the sensitivity of CD11b was 87.69%,the specificity of CD11b was 99.22%,and the area under of ROC of CD11b was 0.991.Conclusion The neutrophils CD11b expression in type 2 diabetes mellitus patients with CMV infection is down regulation.CD11b expression can be used as a laboratory diagnosis basis of CMV infection.

Objective To evaluate the safety and effectiveness of immunosuppression regimens,postoperative complications,dead causes and risk factors of lung transplantation (LTx).Methods The immunosuppression regimens and clinical data of 100 patients with end-stage lung diseases receiving LTx in our hospital were retrospectively analyzed between Sept.2002 to Dec.2010.There were 72 patients subject to single LTx and 28 patients to bilateral LTx,amongst them 61 patients received LTx under circulation support,including 5 cases of cardopulmonary bypass (CPB) support and 56 cases of extracorporeal membrane oxygenation (ECMO) support.The immunosuppression regimens including Cyclosporin,mycophemolate Mofeil and corticosteroids were utilized in 53 recipients before the year of 2007,and Cyclosporin was switched to Tacrolimus in 47 patients after 2007.All the patients received Daclizumab or Basiliximab as immunosuppression induction regimens.Results The 1-,2-,3-and 5-year survival rate after LTx was 73.3％,61.6％,53.5％ and 40.7％ respectively.The mean survival time post-transplant in the patients who received Cyclosporin-based regimens and Tacrolimus-based regimens were (36.57 ± 3.44) months and (35.00 ± 2.33) months,repectively,with no significant differences (P＞0.05).The main causes of mortality included primary graft dysfunction (PGD),acute rejection (AR),bronchiolitis obliterans (BOS) and sepsis.The incidence of AR and BOS in Tacrolimus group was significantly lower than that in Cyclosporin grou (P ＜0.05),but the incidence of diabetes mellitus was significantly higher in Tacrolimus group.Analysis of Spearman rank correlation revealed that there was a direct correlation between the incidence of AR and BOS (r =0.340,P＜0.01).The use of circulation support,diagnosis of IPF,postoperative complications such as AR,BOS and infection were associated with decreased survival time postoperatively,in both univariate and multivariate proportional hazards regression models (P＜0.05).Conclusion Cyclosporin-and Tacrolimus-based regimens were both effective immunosuppression strategies postoperatively,Consummate follow-up surveillance and prompt treatment of complications were the key points in prolongation of survival time and improving quality of life after LTx.

Objective To investigate the correlation between pathogens and spectrum of disease in infants with human cytomegalovirus(HCMV) active infection.Methods A total of 378 cases of HCMV infection diagnosed by the identification of HCMV IgM or PP65 antigen of HCMV.HCMV gB genotyping was carried out by nested PCR and restriction fragment length polymorphism(RFLP) in 107 cases.The results of pathogen,spectrum of disease and clinic feature were analyzed.Results In all 378 infant patients with HCMV,27.78% were systemic infection and 72.22% involved just single organ.Hepatitis,HCMV inclusion disease,thrombocytopenic purpura,pneumonia were pre- dominant with 33.07%,27.78%,13.49%,6.35% respectively.The rate of HCMV inclusion dis ease in infants younger than 2 weeks was higher than in those aged from 3 12 weeks(P ~ 0.05) and children older than 12 weeks(P＜0.01).Infants with higher rate of PP65 antigen positive cells were apt to systemic infection than those with lower rate of PP65 positive cells(P＜0.01).Infants,who were positive by detections of all three methods,were apt to systemic infection than others(P＜0.01). Moreover,infants positive of IgM and PP65 antigen were apt to systemic infection than those just positive by one of the two methods(P＜0.01).The result of gB genotype analysis in 107 cases showed 53 cases of gBⅠ,20 of gBⅡ.18 of gBⅢ.7 of gBⅠ+gBⅡ,5 of gBⅠ+gBⅢand 4 of gBⅡ+gBⅢ,and gBⅣwas not found.Conclusion HCMV could infect multiple organs and have some different clinic features.Combination of different methods can increase the sensitivity to detect the pathogen.The gBⅠgenotype is most prevalent in these infants.

Child, Preschool ; Humans ; Infant ; Infant, Newborn ; Lung ; physiopathology ; virology ; Male ; Metapneumovirus ; isolation & purification ; Paramyxoviridae Infections ; diagnosis ; physiopathology ; Respiratory Function Tests ; Respiratory Syncytial Virus Infections ; diagnosis ; physiopathology ; Respiratory Syncytial Viruses ; isolation & purification

Objective:To investigate the craniofacial features in patients with cleidocranial dysplasia (CCD).Methods:The facial features,cervical vertebral bone age and skeletal abnormalities of 8 patients with CCD were studied by analyzing facial photos,cephalo-metric and panoramic radiographs.Results:4 patients were in the early growth stage and the other 4 in the late period of development. The bossing forehead and inclined eye fissure were observed in all patients,but underdevelopment of midfaces were not obviously pres-ented in younger patients.Morphological abnormalities of craniofacial bones,such as ascending ramus,coronoid process,nasal bones and disappearence of gonial angle were observed in all patients.Conclusion:Some craniofacial malformations in patients with CCD may be presented earlier than underdeveloped midface,which can be helpful for early diagnosis of CCD.