1.Effect of thymopeptide on LPS-induced release of nitric oxide from mouse peritoneal microphase in vitro
Chinese Journal of Biochemical Pharmaceutics 2001;22(2):74-75
Purpose The aim is to investigate the effects of thymopeptide on LPS-induced release of nitric oxide (NO) from mouse peritoneal microphase in vitro.Methods NO was detected by Griess reagent.Results Thymopeptide 83.3~333.3 μg/ml reduced NO synthesis and secretion from mouse peritoneal microphase in vitro significantly.Conclusion The effects of thymopeptide on LPS-induced release of NO from mouse peritoneal microphase may be one of mechanisms of its pharmacological effect.
2.Application of phototherapy and its related problems on neonatal hyperbilirubinemia
International Journal of Pediatrics 2013;(3):272-274
Phototherapy is the most common and effective method to intervene and treat neonatal hyperbilirubinemia in clinic.The application of phototherapy varies from gestational age,birth weight,age and many other factors.Beside the traditional phototherapy instruments,more advanced high-intensive phototherapy instruments are applied to phototherapy.With the increasing application of phototherapy,many problems have showed up.This paper reviews based on the research of the indication,clinical application and adverse reactions on phototherapy.
3.Preliminary cbservation on the safety of Sumianxin Ⅱ combined with Diazepam for anesthesia in the periodontal surgery in Beagle dogs
Journal of Chongqing Medical University 2007;0(09):-
Objective:To observe the anesthetic effects of SumianxinⅡ combined with diazepam on the Beagle dogs in experimental periodontal surgery to Provide the reference. Methods:Healthy adult male Beagle dogs were anesthetized with SumianxinⅡat the doses of 0.05、0.1、0.15 ml/kg in the combination group and diazepam(0.1 ml) by hip intramuscular injection simultaniously. Added injection dosage was half of the first dosage. Healthy adult Beagle dogs in the contral group were only anesthetized with SumianxinⅡ at the doses of 0.05、0.1、0.15 ml/kg by hip intramuscular injection. The time of adding anesthetic was not sure in surgery. Anesthesia induction period,initial duration of anesthesia,and adverse reactions after anesthesia were compared between the two groups. Results: The combined treatment group induction dose respectively (11.00?1.41) min,(9.00?0.82) min,(12.75?0.50) min,For the first time maintaining anesthesia(29.75?6.62) min,(46.00?5.29) min,(45.25?6.02) min,Intraoperative additional one times longer cansustain anesthesia for(38.75?3.50) min,(45.25?4.03) min,(37.25?2.75) min. Pure Sumianxin Ⅱ new standards of sleep narcotic induction respectively respectively was (15.75?2.50) min,(14.25?2.99) min,(14.50?2.08) min,For the first time maintaining anesthesia was (20.75?5.19) min,(20.5?3.42) min,(22.25?5.19) min,the time of adding anesthetic was not sure in surgery. 2 Dogs died in SumianxinⅡ group. United drug group simple Sumianxin Ⅱ induction period in each group by t test,Sumianxin Ⅱat the doses of 0.1 ml/kg supplemented with 0.3 ml dose stability (P
4.Effect of NGF on Release of NO From Peritoneal Macrophages in Mice
China Pharmacy 2001;0(07):-
OBJECTIVE:To investigate the effect of NGF on release of NO from peritoneal macrophages in mice METHODS:The content of NO in macrophages was detected with Griess agent RESULTS:NGF,in concentration of 0 73mg~5 83mg free amino acid per ml,could significantly inhibit release of NO from peritoneal macrophages in mice CONCLUSION:Inhibiting NO release may be one of the mechanism of NGF in protecting neurons
5.FEASIBILITY OF SCREENING FOR ANTIBODY TO HIV BY POOLING THE SERUM SPECIMENS
Chinese Journal of Blood Transfusion 1988;0(01):-
The feasibility of screening for antibody to HIV by pooling 10 serum specimens was demonstrated in this experiment. The test was conducted using the reagent developed by Organon Teknika corporation (U. S. A) for testing for antibody to HIV by ELISA. The determination was made by changing only the ratio between the serum specimen volume and the serum specimen diluent volme, with their total volume being constant. When the serum specimen volume was increased from 5to 59?l as a result of pooling 10 serum specimens, while the serum specimen diluent volume was correspondingly decreased, the optical density of the pooled serum specimens was somewhat raised, but it was always below cutoff value, indicating that the specificity of test did not be subjected to any influence. In addition, when one serum specimen estabished as being positive intesting for antibody to HIV, and nine serum specimens from normal donors were pooled, the serum specimen containing the antibody to HIV had exactly been shown through multiple paired tests, indicating that the serumpooling method did not have any influence on the test sensitivity and could reach the level of original method. The serum-pooling method could be easy to spread because of its many advantages, such as saving the expenditure by 10 times, saving the manpower and material resource, and saving the working hours. (Original article on page 5)
6.Isoknetic Dynamometric Technique for the Quantitative Assessment of Spasticity
Chinese Journal of Rehabilitation Theory and Practice 1999;5(2):75-79
Spasticity is a common problem in upper motor neuron lesions.Assessment of spasticity is extremely valuable in quantifying the effects of a therapeutic intervention.But it is,in other hand,a difficult and challenging problem.Objective:The present study was undertaken to determine the feasibility of quantifying spasticity by the pendulum test using the isokinetic dynamometric technique.We suggest that this machine can be used to perform pendulum test to quantify spasticity.Method:We studied the lower limb muscle tone in 34 spastic subjects,and in 9 flaccid paralysis control subjects,and 10 normal control subjects.Result:Typical pendulun test goniograms of each group were obtained and 5 parameters are extracted from the goniograms to evaluate the degree of spasticity. They are:Al(the flexion angle of the first drop)、R1(relaxation index)、R2(amplitude ratio)、T(time of swings)、F(frequency of swings).The correlation coefficients are calculated to determine the reliability of the parameters(>0.90 for three consecutive test;>0.80 for different days).The validity is determined by t test(p<0.01).Conclusion:The method of using isokinetic dynamometric technique combining pendulum test to quantify spasticity is a reliable,valid,accurate and convenient method of assessing spasticity.
7.Protective effect of extracorporeal membrane pulmonary oxygenation combined with cardiopulmonary resuscitation on post-resuscitation lung injury
World Journal of Emergency Medicine 2021;12(4):303-308
BACKGROUND: Cardiac arrest (CA) is a critical condition that is a concern to healthcare workers. Comparative studies on extracorporeal cardiopulmonary resuscitation (ECPR) and conventional cardiopulmonary resuscitation (CCPR) technologies have shown that ECPR is superior to CCPR. However, there is a lack of studies that compare the protective effects of these two resuscitative methods on organs. Therefore, we aim to perform experiments in swine models of ventricular fibrillation-induced CA to study whether the early application of ECPR has advantages over CCPR in the lung injury and to explore the protective mechanism of ECPR on the post-resuscitation pulmonary injury.
METHODS: Sixteen male swine were randomized to CCPR (CCPR; n=8; CCPR alone) and ECPR (ECPR; n=8; extracorporeal membrane oxygenation with CCPR) groups, with the restoration of spontaneous circulation at 6 hours as an endpoint.
RESULTS: For the two groups, the survival rates between the two groups were not statistically significant (P>0.05), the blood and lung biomarkers were statistically significant (P<0.05), and the extravascular lung water and pulmonary vascular permeability index were statistically significant (P<0.01). Compared with the ECPR group, electron microscopy revealed mostly vacuolated intracellular alveolar type II lamellar bodies and a fuzzy lamellar structure with widening and blurring of the blood-gas barrier in the CCPR group.
CONCLUSIONS: ECPR may have pulmonary protective effects, possibly related to the regulation of alveolar surface-active proteins and mitigated oxidative stress response post-resuscitation.
8.Renal involment of malignant tumor and its treatment in childhood.
Chinese Journal of Pediatrics 2012;50(11):801-806
Antineoplastic Agents
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adverse effects
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Child
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Child, Preschool
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Hematopoietic Stem Cell Transplantation
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adverse effects
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Humans
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Kidney Diseases
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etiology
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pathology
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therapy
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Kidney Neoplasms
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etiology
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pathology
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therapy
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Neoplasms
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complications
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therapy
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Paraneoplastic Syndromes
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etiology
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pathology
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therapy
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Radiotherapy
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adverse effects
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Risk Factors
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Tumor Lysis Syndrome
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etiology
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pathology
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therapy
9.Diagnosis and treatment of Fabry disease.
Chinese Journal of Pediatrics 2007;45(2):149-152
Fabry Disease
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diagnosis
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therapy
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Humans