ObjectiveTo observe the effect of early rehabilitation on motor function and prognosis of the patients with spinal cord injury (SCI).MethodsAccording to started date of rehabilitation, 60 SCI patients were divided into the early rehabilitation group (within two weeks after operation) and control group (three months after operation) with 30 cases in each group. The motor function and activities of daily living (ADL) of all patients were evaluated before and 3 months after treatment, and the incidence rates of complications of two groups were compared.ResultsAfter 3 months treatment, the incidence rates of complications often occurred after SCI such as pulmonary diseases, urinary infection, osteoporosis and adductor, extensor spasticity in the lower limbs etc. were lower than those in the control group. There was a significant difference between two groups. The motor function and ADL of the patients in the early rehabilitation group were better than those of patients in the control group ( P<0.05～0.01).ConclusionEarly rehabilitation after SCI can significantly decrease the incidence rate of complications and improve patient's motor function and ADL.

ObjectiveTo observe the effect of early rehabilitation on motor function and prognosis of the patients with spinal cord injury (SCI).MethodsAccording to started date of rehabilitation, 60 SCI patients were divided into the early rehabilitation group (within two weeks after operation) and control group (three months after operation) with 30 cases in each group. The motor function and activities of daily living (ADL) of all patients were evaluated before and 3 months after treatment, and the incidence rates of complications of two groups were compared.ResultsAfter 3 months treatment, the incidence rates of complications often occurred after SCI such as pulmonary diseases, urinary infection, osteoporosis and adductor, extensor spasticity in the lower limbs etc. were lower than those in the control group. There was a significant difference between two groups. The motor function and ADL of the patients in the early rehabilitation group were better than those of patients in the control group ( P<0.05～0.01).ConclusionEarly rehabilitation after SCI can significantly decrease the incidence rate of complications and improve patient's motor function and ADL.

It is generally accepted that locomotion in mammals, including humans, is based on the activity of neuronal circuits within the spinal cord (the central pattern generator, CPG). Recent studies indicate a plastic behavior of the spinal neuronal circuits following a spinal cord injuries (SCI). Studies have shown that body weight support treadmill training (BWSTT) is effective in restoring locomotor function in cats with a complete spinal lesion. It has become possible to regain some locomotor activity in patients with spinal cord injury through an intense training on BWSTT, as in cat. So, it is a staired problem how to take advantage of the plasticity of spinal locomotor pattern generator and design the gait training program for SCI patients.

Common Bile Duct ; surgery ; Gallstones ; surgery ; Humans ; Laparoscopy ; methods ; trends

Objective: To study the relationship between plasma level of N-terminal B-type natriuretic peptide (NT-proBNP) and ventricular diastolic dysfunction in elder hypertensive patients without target organ damage.
Methods: A total of 66 relevant patients treated in our hospital from 2012-03 to 2014-03 were studied. According to the standard of ventricular diastolic dysfunction, the patients were divided into 2 groups: Study group, n=27 patients with diastolic dysfunction and Control group, n=39 patients without diastolic dysfunction. The patients in Study group were further divided into 3 sub-groups based on Doppler classification of diastolic dysfunction:Grade 1, the patients with E/A<1.0, DT≥240 ms, IVRT>90 ms, n=8. Grade 2, the patients with E/A>1.5, DT (150-220) ms, IVRT<90 ms, n=13. Grade 3-4, the patients with E/A>1.5, DT≤150 ms, IVRT<70 ms, n=6. Plasma levels of NT-proBNP and Doppler ultrasound findings were compared to study the relationship between
NT-proBNP and ventricular diastolic dysfunction.
Results: Plasma level of NT-proBNP was higher in Study group than that in Control group. NT-proBNP level in Grade 3-4 sub-group was obviously higher than those in Grade 1 and Grade 2 sub-groups, NT-proBNP level in Grade 2 sub-group was higher than that in Grade 1 sub-group. Pearson correlation analysis indicated that NT-proBNP level was positively related to systolic blood pressure, diastolic function and E/E’ (r=0.211, P=0.037, r=0.442, P=0.004 and r=0.556, P=0.000), while negatively related to E’/A’ (r=-0.372, P=0.000).
Conclusion: The increased plasma level of NT-proBNP are highly support for ventricular diastolic dysfunction in elder hypertensive patients without target organ damage, NT-proBNP level is related to ventricular diastolic function.

This experiment was based on a full-length SAMDC sequence from the drought stress induced cDNA library of Cleistogenes songorica.Two methods,absolute quantification and relative quantification,were used to analyze the gene expression under different tissue and drought stress treatment by real-time quantitaive PCR experiments and were compared each other,while CsSAMDC gene was used as a case.Leaves and roots tissue were sampled from the plants at 0,4,6,8,and 10 d of drought stress and 1 and 4 d after rewatering.The 2-CT method was used to analyze the relative changes in gene expression from quantitative real-time PCR experiments.A standard curve was generated by the purified plasmid DNA.The primer concentration in the realtime PCR was optimized.All absolute quatification data were normalized by the normalization factor from the most stable house-keeping genes in C.songorica.Relative quantification results using 2-CT method showed that CsSAMDC transcripts increased 5.93?0.71 fold in roots,down regulated 0.62?0.13 fold in leaves,under drought stress.Absolute quantification results showed that CsSAMDC transcripts up-regulated in roots significantly under drought stress as 1ong as 8 to 1 0 days after drought stress,while down-regulated in both roots and leaves after drought stress within 6 days and 1 to 4 days after rewatering,compared with control.CsSAMDC transcipt showed similar tendency of gene expression compared by using relative and absolute quantification methods.

ObjectiveTo study the rehabilitation effect of physical therapy on patients with above knee amputation. Methods50 patients with above knee amputation were evaluated with FIM scale before and after physical therapy. ResultsThere was a significant difference before and after physical therapy(P<0.05). Conclusions Physical therapy is effective on patients with above knee amputation.

Pathological pain, normally referring to tissue injury-induced inflammatory pain and nerve injury-induced neuropathic pain, is an expression of neural plasticity. Injuries and intense noxious stimuli result in pain hypersensitivity,which is contributed by peripheral sensitization (increased sensitivity of primary sensory nociceptors) and central sensitization (increased sensitivity of spinal dorsal horn and other CNS neurons). Activation of several protein kinases causes both forms of sensitization via posttranslational regulation, such as phosphorylation of key membrane receptors and channels. In particular, activation of multiple signal cascades converge on the activation of MAPK (mitogen-activated protein kinase).Activation of MAPK family members of ERK and p38 by nociceptive activity, growth factors, and inflammatory mediators in primary sensory and secondary order neurons, not only results in posttranslational modification, but also increases the expression of numerous genes via transcriptional and non-transcriptional regulation. Eventually this activation contributes to the development and maintenance of heightened pain sensitivity following injury.

The pain experience includes a sensory-discriminative and an emotional-affective component. The sensory dimension describes the quality, intensity, and spatio-temporal characteristics of the sensation. The affective dimension refers to the unpleasantness or aversion of sensation. The great progress at the genic, molecular, cellular, and systemic level on the study of the sensory dimension of pain has been made over past four decades. However, to consider only the sensory features of pain, and ignore its motivational and affective properties, is to look at only part of the problem. A line of clinic observations indicate that the patients with chronic pain suffer from much more affective disturbance than pain itself. Obviously, physiological arousal and hypervigilance to pain cause negative affect, such as fear, anxiety, angry, worry, aversion, even tendency of suicide, these negative affective states in turn enhance pain sensation. Therefore, the mechanisms underlying the affective dimension of pain have recently received more attention. In order to deepen and expand our understanding of the nature of pain, this review summarizes the main findings regarding affective component of pain in neuroanatomy, neurophysiology, and cell biochemistry

Objective To observe short-term rapid atrial electrical activation on atrial size and atrial systolic function. Methods Twelve anesthetized mongrel dogs were underwent insertion of a transvenous lead at the right atrial appendage and subjected to 5 hours of rapid right atrial pacing at 450 beat/min. Acoustic quantification(AQ) waveform on biatrial was recorded before and after 5 hours pacing with AQ technique. All measurements were made in sinus rhythm. Results After 5 hours rapid right atrial pacing, end systolic volume(ESV) of both left and right atrial enlarged significantly from ( 5.92 ? 1.51 )ml to ( 7.27 ? 1.86 )ml,from ( 3.13? 0.71 )ml to ( 3.58 ? 0.73 )ml, respectively; but biatrial function decreased markedly,left and right atrial ejection fraction decreased from ( 51.03 ? 9.45 ) to ( 38.23 ? 10.52 ),from ( 36.76 ? 5.36 ) to ( 30.71 ? 7.97 ), respectively. However, left atrial ESV enlarged more than right atrial (by 23 vs 8,P