Objective To understand the air microorganisms pollution and the community structure and distribution characteristics of outdoor airborne microbes in Xi'an in autumn.Methods Airborne microbes were sampled by fallen plates method in autumn,2006 in Xi'an at three typical sites,factories region,garden greenbelt region and main traffic region.Results The results showed that the total concentration of airborne microbes at the factories region and garden greenbelt region,main traffic region was 2 500 cfu/m~3,1 354 cfu/m~3 and 4 404 cfu/m~3 respectively.Bacteria and fungal genus were predominant.Gram positive bacteria were much more than Gram negative bacteria.There were 20 genera of bacteria and 8 fungal genus were detected. Micrococcui,Bacillus,Staphylococcus and Pseudornonus were predominant.The most frequently isolated fungal genus were Penicillium,Paecilomces,Cladosporium and Nonsporulatiny mycelia.The community structure of airborne fungal genus and the dominant genus in different function region was significantly different.The distribution characteristic of airborne microbe concentration in different function region was different.The concentration of airborne bacteria was significantly lower in garden greenbelt region than factories region and main traffic region,whereas the concentration of airborne fungi in garden greenbelt region was higher than that in factories region and main traffic region.Conclusion The distribution of the airbome microbes shows a characteristic of time and space,it is affected not only by the environment and pollution,but also by the other factors such as human activities,animals and plants.

Objective To investigate the role of survivin and P53 in apoptosis of gastric adeno- carcinoma,as well as its relationship with the clinicopathologic features and the prognosis.Methods The gas- tric tissue microarrays were composed of those from 100 cases of gastric cancer and 30 controls.At these tissue microarrays,expressions of survivin and P53 were investigated immunohistochemically,and tumor cell apoptosis index was examined by TUNEL method.Of the 100 cases,47 cases were followed-up from 14 months to 13 years,in which the survival was analyzed.Results Two paraffin-embedded gastric carcinoma tissue micro- arrays were successfully constructed,including 114 and 116 tissue spots,respectively.Immunohistochemical analysis showed that survivin was expressed in 78 cases (78%).No expression of survivin was detected in control tissue (P0.05).In the 47 cases with followed-up data,univariant analysis revealed that the survival was correlated with invading of vessel and nerve,TNM stages,and expression of survivin.The histological grades and expression of P53 were not related to prognosis.However,Cox stepwise proportional hazards analysis showed that only TNM staging and survivin status retained significant independently in prospecting prognosis.Conclusions The expression of survivin was associated with the pathologic features,TNM stages and prognosis in gastric carcinoma,indicating that overex- pression of survivin may be a poor prognosis factor for gastric carcinoma.

Human erythropoietin receptor (hEPOR) plays an important role in regulating the red blood cell production by promoting the proliferation and differentiation of RBC from erythroid precursors, hEpoR is a transmembrane protein, and its extrocellular domain (sEpoR) is of great importance in Epo signal transduction pathway. We cloned the gene of sEpoR by RT-PCR from the total RNA of human fetal liver and expressed it in E. coli after insertion of the gene in the expression vector pBV220. The cloned gene was confirmed by sequencing analysis and gene product was confirmed by both Western blot and its first 11 amino acid residues sequence of the N-terminal. In vitro bioassay showed that the purified gene product can repress the growth of TF-cells in the presence of Epo.

This study deals with colony expansion characteristics, biomass increment and Na accumulation in mycelia of three ectomycorrhizal fungi ( Boletus edulis, Xerocomus chrysenteron and Gomphidius viscidus ) under treatment of Na2SO4 and NaCl. The experimental results showed that no impact was observed from Na2SO4 and NaCl treatments on the growth mode of the mycelia, but the biomass increment of X. chrysenteron and G. viscidus was significantly restrained under 0.1 mol/L Nacl. Under the treatment of Na2SO4, biomass increment of X. chrysenteron is significantly higher than the control, but the biomass of G. viscidus is lower than that of the control, and no significant impact was measured on growth of B. edulis under both treatments of Na2 SO4 and NaCl. The experiment also indicated that Na accumulation in mycelia varied significantly among the three tested strains, highest Na accumulation was measured in X. chrysenteron under treatment of NaCl, while under the treatment of Na2SO4 , accumulation of Na in B. edulis is much higher than the other two strains.

The teaching for medical advanced students is a routine and important task in teaching hospital.To increase the teaching quality,we should emphasize professional theory learning,increase clinical skill training,strengthen medical records writing training and enhance emotional exchange.

0 05)in all parameters The tumor inhibitory rate and the cells detained in G 0～G 1 phase significantly increased, meanwhile, cells in S phase, the PI and PCNA significantly decreased in rhGH+L OHP group compared with control group or rhGH group ( P

MicroRNA (miRNA) belongs to a class of small, non-coding RNA molecules that contains 18-25 nucleotides and regulates gene expression at post-transcriptional level. Many miRNAs are highly conserved and display timing- and tissue-specific expression. With the advance of the miRNA detection technologies, miRNA has been introduced to forensic science as a potentially novel set of genetic markers of forensic body fluid identification, species identification and PMI estimation. In this article, the detection methodologies of miRNA are reviewed, and their potential applications in forensic practice and research future are also discussed.
Body Fluids ; Forensic Medicine ; Genetic Markers ; Humans ; MicroRNAs

Objective To study the effects of xanthoxylin on platelet aggregation and freeCa2+ level induced by CaCl2 and A23187. Methods Platelet aggregation and free Ca2+ level in the platelet cytosol were measured by turbidimetry and fluorimetry of Fura-2/AM. Results Xanthoxylin at the concentration of 0.037, 0.185, 0.924, 9.240, 92.40 μmol/L significantly inhibited CaCl2 and A23187-induced platelet aggregation, with the inhibition rates ranging from 9.0% to 67.1% for CaCl2 and from 18.1% to 72.5% for A23187. A23187-induced free-Ca2+ level in the platelet cytosol was also significantly reduced (from 12.8% to 63.4%). Conclusion The inhibition rate of platelet aggregation was positively correlated with the reduction rate of cytosolic free-Ca2+ level by xanthoxylin.

Objective To study the effects of xanthoxylin on platelet aggregation and freeCa2+ level induced by CaCl2 and A23187. Methods Platelet aggregation and free Ca2+ level in the platelet cytosol were measured by turbidimetry and fluorimetry of Fura-2/AM. Results Xanthoxylin at the concentration of 0.037, 0.185, 0.924, 9.240, 92.40 μmol/L significantly inhibited CaCl2 and A23187-induced platelet aggregation, with the inhibition rates ranging from 9.0% to 67.1% for CaCl2 and from 18.1% to 72.5% for A23187. A23187-induced free-Ca2+ level in the platelet cytosol was also significantly reduced (from 12.8% to 63.4%). Conclusion The inhibition rate of platelet aggregation was positively correlated with the reduction rate of cytosolic free-Ca2+ level by xanthoxylin.

IgG4-related disease (IgG4-RD) is a newly recognized fibro-inflammatory condition of autoimmune etiology in recent twenty years, mainly manifesting as mass-forming lesions in single or multiple organs. In the past, it was often missed or misdiagnosed as inflammation or tumor. Patients may die from multiple organ failure due to end-stage fibrosis if they are not treated promptly. However, the number of clinically confirmed cases has gradually increased with the improvement of diagnostic level in recent years, and these patients have benefited greatly after receiving early treatment. Although patients generally respond well to traditional immunosuppressors including glucocorticoids and disease-modifying anti-rheumatic drugs, refractory and recurrent cases, even patients with glucocorticoid contraindication are common. Important mechanistic insights have been derived from studies of B-cell depletion therapy, but greater awareness of the pathophysiology of IgG4-RD is still badly needed to identify novel therapeutic targets. In this article, we reviewed the pathogenesis progress and promising therapy of IgG4-RD to seek better clinical management of IgG4-RD.