This aticle introduced the process of Botulinum toxin from toxin to drug, as well as the application in Spastic Cerebral Palsy such as dose, therapeutic estimation and side-effect etc.

Brucellosis ; diagnosis ; drug therapy ; Child ; Child, Preschool ; Female ; Humans ; Male

AIM: To establish the quality standard for Qingdai Powder (Indigo Naturalis, Radix Glycyrrhizae, Borax, Boreolum Syntheticum, Herba Menthae, Rhizoma Coptidis, Catechu, etc.) METHODS: Indigo Naturalis, Borneolum Syntheticum, Rhizoma Coptis, Radix Glycyrrhizae were indentified by TLC, and the content of indigo was determined by HPLC. RESULTS: Indigo Naturalis, Borneolum Syntheticum, Rhizoma Coptis, Radix Glycyrrhizae could be indentified by TLC. Indigo showed a good linear relationship at a rang of 27.35～ 410.25ng, r=0.9997. The average recovery of indigo was 99.03%( n =6) and RSD was 2.14%. CONCLUSION: The method is simple, accurate and high specificity with good repeatability. The method can be used for quality control of Qingdai Powder.

Objective Aliginic sodium diester(ASD) liposome was prepared and its aggregation stability was evaluated.Methods ASD liposome was made by reverse phase evaporating method from egg yolk lecithin,compared with blank liposome,the size distributions and mean particle diameters of ASD liposome and blank liposome were measured respectively before and after 40 hours heating under 37℃.Results Under the condition of n(PC)∶n(CHOL) = 1∶1,the mean size diameter of ASD liposome was 4.24?m.The mean size diameter of ASD liposome changed only 0.26?m after cultivation,however,the value of blank liposome was 1.35?m.Conclusion ASD loaded in the liposome enhanced the physical stability of the liposome.

Objective To determine the expression of cancer stem cell markers, CD133, CD44 and OCT-4, in small cell lung cancer cell line NCI-H82 and confirm the specific markers. Methods NCI-H82 cells were cultured, and the expressions of markers (CD133, CD44 and OCT-4) were detected by immunofluorescence staining. The immunohistochemistry was performed to detect the expressions of CD133, CD44 and OCT-4 in 79 tissues with small cell lung cancer. Results In NCI-H82 cells, the florescence was positive in CD133 and CD44 and negative in OCT-4. In the tissues, the expression of CD133 and CD 44 was related with tumor size, lymph node metastasis and clinical stage (P< 0.05), while OCT-4 was negative expression. Conclusion CD133 and CD44 might be the cancer stem cell markers of small cell lung cancer, which maybe have clinical significance on diagnosis and treatment.

Studying classic cases is one of the applicable methods for student's self-learning,especially at clinical rotation period.Seven-year program pediatric students (2004 grade) from pediatric medical collage,Capital Medical University of China shared classic cases from New England Journal of Medicine (NEJM),reported clinical cases they took part in and summarized their writing of case report.Through these measures,students felt the significance of studying classic cases and noticed that case study played an important role in training of clinical reasoning,multidisciplinary approach,as well as self-learning ability for medical students.

Animals ; Dentistry ; Swine ; Swine, Miniature

Animals ; Collagenases ; metabolism ; Dental Bonding ; Dental Caries ; enzymology ; Dentin ; enzymology ; pathology ; Gelatinases ; metabolism ; Humans ; Matrix Metalloproteinase 14 ; metabolism ; Matrix Metalloproteinase 20 ; metabolism ; Matrix Metalloproteinase 3 ; metabolism ; Matrix Metalloproteinases ; metabolism ; Sclerosis

Objective To study the utilization of DSA for interventional examination and therapy in acute ischemic cerebrovascular disease. Methods The summarization of properly utilizing the DSA equipment and events happening in the process were analyzed after application on 550 cases with acute ischemic cerebrovascular attacks. Results Application of perfusion DSA is useful to obtain rapid diagnosis and evaluation of interventional therapeutic efficacy for acute ischemic cerebrovascular disease. The practical projection angle can display the features and extent of vasculopathy clearly, and offer the best operative position for promotion of rotation DSA and the diagnostic imaging and therapeutic efficacy, three-dimensional reconstruction are usful to the success of interventional management. Imaging measuring technique of DSA would provide precise data for doctors to choose the accurate intervention materials. Conclusion Reasonable utilization of DSA equipment plays an important role in interventional therapy of acute ischemic cerebrovascular disease, and also is the gold standard for displaying images for rapid diagnosis.

The incidence of menopausal anxiety and depression is increasing. It can induce and aggravate a variety of somatic symptoms. Despite of the good effects of psychotropic drugs on the disease, patients' compliance is poor. Therefore, it is necessary to find a drug which is practical, effective, and easy for patients to take.