Objective To study medical fear and its influencing factors in hospitalized school-age children in Lanzhou area.Methods Children's Medical Fear Scale was used to investigate 110 cases of hospitalized school-age children,and the investigation result underwent analysis.Results The total score of children's medical fears was (50.43±7.34),the three items with the highest score among 30 items were surgery,death,being deprived of education,homework delay,surgery and death tied for first.Conclusions Medical fear of school-age children was related with age and gender,female was significantly higher than that of male.No relationship with the family education environment,duration of illness,area of residence.

OBJECTIVE:To study the anti-oxidative effects of spirulina kinase (SPK) in vitro. METHODS:The methods of phenanthroine-Fe2+ oxidation method,DPPH and auto-oxidation of pyrogallol were used to measure the effects of different concen-trations of SPK on scavenging hydroxyl (OH-) free radical,DPPH free radical and superoxide anion (O2-) free radical;IC50 of SPK was calculated. Prussian blue reaction was used to determine total reducing ability(by absorbance)of different concentrations of SPK to Fe3+. Vitamin C(VC)was used as positive control in above trials. RESULTS:SPK could eliminate the OH-free radical, DPPH free radical and O2- free radical in concentration-dependant manner,and the maximum elimination rate of SPK to OH- free radical and DPPH free radical were 86.82% and 78.98%(IC50 were 54.31,0.636 g/L),which were higher than VC (64.77%, 73.49%). The maximum elimination rate of SPK to O2- free radical was 78.31%(IC50 was 3.918 g/L),which was lower than VC (94.14%). In reducing ability test,SPK improved absorbance in reducing ability test system,and maximum absorbance was simi-lar to VC in concentration-dependant manner. CONCLUSIONS:SPK has obvious anti-oxidant activities in vitro.

Objective:To systematically analyse the blood coagulation features of coronavirus disease 2019 (COVID-19) patients.Methods:An electronic search in PubMed, Scopus, Web of Science, EMbase, and CNKI to collect studies related to the blood coagulation features of COVID-19 patients from 1 January 2020 to 1 May 2020. Two reviewers independently screened literatures, extracted data and assessed the risk of bias of included studies. Then, the platelet count, D-dimer value, prothrombin time (PT), activated partial thromboplastin time (APTT) and fibrinogen of patients with different types of diseases were analyzed by using Stata12.0 software.Results:Thirty-nine retrospective studies involving 6 994 COVID-19 patients were included. The results of meta-analysis showed that:(1) compared with severe group, the platelet count (Weighted mean difference; WMD=20.11, 95% CI 11.53 to 28.69, P<0.001) and APTT (WMD=1.30, 95%CI 0.31 to 2.30, P=0.01) were found to be higher while D-dimer (WMD=-0.41, 95%CI-0.58 to-0.24, P<0.001), fibrinogen (WMD=-0.58, 95% CI-0.76 to-0.39, P<0.001) and PT (WMD=-0.51, 95%CI-0.92 to-0.10, P<0.001) were lower in mild group; the platelet count (WMD=-14.75, 95% CI-29.73 to-0.23, P=0.044) was found to be lower while D-dimer (WMD=1.06, 95% CI 0.65 to 1.47, P<0.001) was found to be higher in critical ill patients. (2)Compared with the survival group, the patients in death group displayed elevated levels of D-dimer (WMD=6.86, 95% CI 4.15 to 9.57, P<0.001) and PT (WMD=1.37, 95% CI 0.73 to 2.02, P<0.001) while platelet count (WMD=-36.40, 95% CI-63.23 to-9.58, P=0.008) remained low. Conclusion:Coagulation dysfunction was common in severe, critical ill and dead COVID-19 patients. Platelet count, D-dimer and PT levels were associated with the severity of the disease, and thus could be used as early warning indicators for the deterioration of the disease during hospitalization.

Objective To predict the biological process and signaling pathways in which hsa-miR-1908 might be in-volved by a series of bioinformatics analysis, so as to lay foundations and provide theoretical basis for the further studies of hsa-miR-1908 biological function in human preadipocytes. Methods The sequence of hsa-miR-1908 was acquired from miR-Base database, and target genes of hsa-miR-1908 were predicted by miRanda, and then the intersection of the results and the results of gene-chip as gene set were further analyzed by gene ontology and pathway enrichment. Results The hsa-miR-1908 had some conserved property among different species. The functions of the target genes were enriched in Wnt receptor signal-ing pathway through beta-catenin, cell cycle, cell apeptosis and other biological processes. The GnRH signaling, MAPK sig-naling, insulin signaling, cell cycle signal transduction pathways and signaling pathway in pancreatic cancer were signiifcantly enriched. Conclusions The target genes set of hsa-miR-1908 were enriched in multiple biological process which are related with the obesity. This study provides guidance for the further study in human preadipocytes.

Objective: To explore the forces of lower limbs during side slip and forward slip through biomechanical study, and to provide a theoretical basis for the development of lower limb movements. Methods: BTS SMART DX motion capture analysis system, 8 infrared high-speed cameras, Kistler three-dimensional dynamometer, BTS FREEEMG 1000 portable wireless surface electromyography system were used to collect real-time data of side slip and forward slip. SPSS 20.0 univariate analysis of variance (ANOVA) was used to compare the differences of indicators between different groups. Results: In the side sliding movement, there were significant differences in hip motion (P<0.01) between 3-year-old group and 5-year-old group, hip motion(P<0.01) between 4-year-old group and 5-year-old group, knee motion(P<0.05) and ankle motion(P<0.05) between 3-year-old group and 5-year-old group. There was a significant difference in hip motion(P<0.05) between 3-year-old group and 5-year-old group. The Fx force(P<0.01) and vertical Fy force(P<0.01) in the left and right directions of the 3-year-old group and the 5-year-old group were significantly different in the side-slip movement, while the Fx force(P<0.01) in the right and left directions of the 3-year-old group and the 5-year-old group were significantly different in the forward-slip movement, and the vertical Fy force(P<0.05) was significantly different. There were significant differences in the long head of biceps femoris, gastrocnemius and anterior tibial muscle between 3-year-old group and 5-year-old group (P<0.05). There were significant differences in the middle gluteal muscle, gracilis muscle, adductor magnus, pubic muscle, medial femoral muscle and soleus muscle between 3-year-old group and 5-year-old group (P<0.05). There was significant difference in the anterior tibial muscle (P<0.05) between the 3-year-old group and the 5-year-old group in the supporting stage, and significant difference in the gastrocnemius muscle(P<0.05) during the swing stage. There was significant difference in the anterior tibial muscle(P<0.05) between the 3-year-old group and the 5-year-old group in the swing stage. Conclusion The development of side slip and forward slip in children aged 3 to 5 years old showed that the results suggest motor development increases significantly with age among preschoolers. Rhythmic movements of side slip and forward slip should be selected in the critical period of children’s movement development at different ages, laying the foundation for good physical and mental development.

Objective To construct profiles of health status based upon physical,mental and social support items in adult twins of Qingdao.Methods Grade of Membership(GoM) model was applied to a set of 31 indicators to construct ideal profiles.Results Four health profiles were identified: pure type Ⅰ(healthy),pure type Ⅱ(personality disorders),pure type Ⅲ(psychological symptoms) and pure type Ⅳ(physiological symptoms).The most frequently occurring combination in this population was profile Ⅰ,Ⅱ,Ⅳ(14.74%),followed by profile Ⅰ,Ⅱ,Ⅲ,Ⅳ(13.44%),and then type Ⅰ(11.08%).Only 13.56% of subjects fell completely into one single pure type.Conclusions One healthy type and three non-healthy types are determined.Most individuals exhibit some of the characteristics of two or more types,holding partial membership in multiple categories.

Humans ; Deglutition ; Time Factors ; Language

The effects of Zn2 + on GABAA-receptor mediated responses in acutely isolated rat sacral dorsal commissural nucleus (SDCN) were studied using nystatin-perforated whole cell recording techniques.The results demonstrated that ( 1 ) GABA induced inward currents through activation of GABAA-receptor at a holding potential of -40 mV; (2) GABAA-receptor mediated responses were suppressed by Zn2 + in a reversible and voltage-independent manner; and (3) in the presence of Zn2 + , the concentration-response curve of GABA-induced responses was shifted to the right in a parallel manner. The results suggest that Zn2 +allosterically depresses GABAA-receptor mediated currents.

Objective To explore the risk factors for septicemia after cesarean section,and provide reference for clinical prevention of postoperative septicemia.Methods Clinical data of patients who underwent cesarean section in a maternal and child health hospital between January 1,2013 and October 31,2016 were collected by retrospective survey method,risk factors were analyzed by multivariate logistic regression model.Results A total of 4 604 cases of cesarean section were selected,32 cases of septicemia occurred,incidence was 0.70％.Multivariate logistic regression analysis showed that there were seven independent risk factors for septicemia:gestational diabetes mellitus (OR =4.03),trying vaginal delivery(OR =15.86),No.of vaginal examination ≥3 times(OR =6.77),premature rupture of membrane≥12 hours(OR =3.47),intra-operative bleeding≥1 000 mL(OR =4.66),postoperative duration of indwelling urinary catheter≥24 hours(OR =2.83),and antimicrobial use within a week(OR =3.20).Four factors were protective factors:gestational weeks≥34 weeks(OR =0.20),hemoglobin≥100 g/L(OR =0.40),albumin≥35 g/L(OR-0.28),and amniotic fluid volume at a normal level(OR =0.22).Conclusion It is possible to prevent and control the occurrence of septicemia after cesarean section through strict management of independent risk factors and intervention in protective factors of pregnant women during peri-operative period.

OBJECTIVETo study the levels and roles of cytokines TNF-α, IL-6 and IL-10 in bronchoalveolar lavage fluid (BALF) in children with Mycoplasma pneumoniae pneumonia (MPP).

METHODSThe levels of TNF-α, IL-6 and IL-10 in BALF were measured using ELISA in children with MPP at acute stage (n=45) and at remission stage (n=30). Twenty children without lung lesions severed as the control group.

RESULTSThe TNF-α, IL-6 and IL-10 levels in BALF were higher in children with MPP at acute stage than those in the control group (P<0.05). The levels of TNF-α and IL-6 in BALF at remission stage were reduced to the levels similar to the control group and were significantly lower than those at the acute stage in children with MPP. However, the levels of IL-10 in BALF remained at higher levels at remission stage in children with MPP.

CONCLUSIONSThe levels of TNF-α, IL-6 and IL-10 in BALF increase in children with MPP at acute stage, suggesting that the cytokines may be involved in the pathogenesis of MPP.

Adolescent ; Bronchoalveolar Lavage Fluid ; immunology ; Child ; Child, Preschool ; Female ; Humans ; Interleukin-10 ; analysis ; Interleukin-6 ; analysis ; Male ; Pneumonia, Mycoplasma ; etiology ; immunology ; Tumor Necrosis Factor-alpha ; analysis