Objective To establish a rat model of chronic renal failure (CRF) by using 5/6 subtotal nephrectomy method , then observe and evaluate the effect of Turbidity‐reducing Granule on the intestinal immune function of rats with CRF .Methods CRF model Wistar rats were divided into high ,medium and low dose group ,sham operation group ,and model control group .respec‐tively given Turbidity‐reducing Granule 4 .05 ,8 .10 ,16 .20 g · kg -1 · d-1 .Benazepril group was given 2 mL/d (1 .2 mg/d) Benaze‐pril suspension fed;model control group and the sham group are saline 2 mL/d orally .The rats were observed before and after treat‐ment of signs and general condition ,serum BUN ,Scr changes ,and pathological changes were observed under microscope .ELISA method were used to detect serum and intestinal SIgA .Results Turbidity‐reducing Granule reduced the level of serum BUN ,Scr in rats with CRF ,there were statistically significant differences of Benazepril group and high ,medium ,low dose of Turbidity‐reducing Granule group compared with model group(P<0 .05) .Compared with the model group ,the intestinal SIgA of Benazepril group and low ,middle ,high dose of group increased significantly(P<0 .05);intestinal SIgA in ,middle and high dose group were significantly increased compared with Benazepril group(P<0 .05) .Conclusion Turbidity‐reducing Granule could improve renal function and in‐testinal immune function .

Blast Injuries ; Deafness ; etiology ; Humans

Most pelvic fractures were commonly seen in polytrauma caused by violent forces,and often complicated with hemorrhagic shock,with mortality being 25%～39%.Anti-shock and control of bleeding are th e philosophy of treatment of this critical condition.Since 1985,hundred s of pelvic fracture victims have bee n sal-vaged in my hospital by VIP procedure which means ventilation(V),infusion(I),and pulsation(P).First,V was to establish an airway,then I was to manage hypovolumic shock,and fin ally,P was to improve the impaired heart function to maintain normal hemodynamics and tissue perfusion.It was necessary for antishock therapy to infuse rapidly and sufficiently in o rder to gain diluted blood.Pneumatic antishock garment,angiography an d em-bolism,and retroperitoneal tampon aded bleeding could control most hem orrhage associated with pelvic fractures.[

Objective To evaluate the radiofrequency catheter ablation for type I atrial flutter through application of Holo catheter labelling with anatomic imaging localization to ablate the isthmus of IVC TA during complete double way block.Methods Eleven cases with type I atrial flutter undergone Holo catheter labelling technique and consecution with conduction time change of coronary venous sinus orifice withright atrial lower lateral wall pace excitation ,were performed with radiofrequency catheter ablation for the isthmus outcoming with complete double way conduction block. Results All together 11 cases with 4 of atrial flutter and 7 of sinus rhythm were undergone radiofrequency catheter ablation resulting with double way conduction block of the isthmus accompanied by prolongation of right atrial conduction time 56.0?2.3ms and 53.0?4.6ms respectively.The right atrial excitation consecution during coronary venous sinus orifice and right atrial lower lateral wall pace excitation appeared to be in clockwise and counter clockwise of single direction. No recurrence occurred during 3-34 months follow up with only one showing atrial fibrollation.Conclusions The application of Holo catheter labelling technique with anatomic imaging localization to achrieve the double way conduction block by radiofrequency catheter ablation of TVC TA isthmus, is a reliable method for treating atrial flutter.

Next generation sequencing (NGS),also known as massively parallel sequencing (MPS),with significant advantages such as high throughput,rapidness,integration and low cost,has been widely used in research and clinical diagnosis fields and promises bright future for forensic applications.Compared with traditional DNA sequencing methods,NGS technologies allow the simultaneous detection of various forensic genetic markers.The classical CE-based STR genotyping method only can differentiate alleles with length polymorphism.However,there are occasions like DNA base substitution and repeat sequence variations,which give different alleles with the same size of amplicons.These alleles are heritable from parents to off-springs,and could be of great importance for solving criminal cases.NGS-based STR genotyping can be compatible with current STR data output mode,and allows for the full resolution of STR loci by providing additional sequencing polymorphism information.In this article,the authors focused on the forensic STR genetic markers and next generation sequencing,and reviewed recent progresses on NGS-based STR genotyping.Perspectives of NGS-based full resolution STR genotyping were presented and possible challenges were discussed in the hope of providing a reference for related studies and applications.

Bronchogenic Cyst ; Child ; Humans ; Male ; Neck ; pathology ; Young Adult

Objective To study the etiology,pathogenesis,diagnosis and treatment of non-traumatic splenic rupture.Method The clinical data of patients with atraumatic splenic rupture from our hospital were retrospectively analyzed,and the liferatures were reviewed.Results Most cases were male.The average age was high.The etiological factors were diverse,and the most common being blood diseases,virus infection,local inflammation and neoplasm.The pathogenesis is related to splenomegaly,increase in splenic fragility,altered haemostatic mechanisms,malposition of the spleen,and violent contraction of the diaphragm.It is more difficult to arrive at a diagnosis when compared with traumatic splenic rupture.Most patients required splenectomy,especially when the etiological factors were malignant blood diseases or splenic tumors.Some patients received non-operative treatment.The prognosis was related to the etiological factors and age.Conclusions The etiological factors of non-traumatic splenic rupture were diverse,and pathological splenic rupture was most common.A preoperative diagnosis was difficult,and splenectomy was the most common treatment.

Objective To discuss the protective effect of carbon nanoparticles lymphatic tracer on parathyroid glands in the operation of thyroid carcinoma. Methods Retrospectively analyzed the clinical data of 65 thyroid carcinoma patients from January 2014 to October 2014, and they were divided into 2 groups according to whether injected with carbon nanoparticles. The experimental group (30 cases) were injected with carbon nanoparticles in thyroidglands before major procedure, while the control group (35 cases) were not injected. In the experimental group, there were 22 cases accepted total thyroidectomy of the affected side gland + isthmus thyroidectomy + subtotal thyroidectomy of the offside gland, and 8 cases of total thyroidectomy. In the control group, there were 25 cases accepted total thyroidectomy of the affected side gland + isthmus thyroidectomy + subtotal thyroidectomy of the offside gland,and 10 cases of total thyroidectomy. And all the 65 cases ac-cepted centrallymph nodes dissection. Results After carbon nanoparticles injection, central lymph nodes became black while parathyroid glands did not become black. The experimental group showed (2. 6 ± 0. 8) parathyroids each patient, and no parathyroid was cut by mis-take;the control group showed (1. 9 ± 0. 7) parathyroids each patient, and 3 parathyroids were cut by mistake. and the difference between the two groups was statistical meaningful (P<0. 05). There was no permanent hypoparathyroidism in the two groups. There was 1 cases of temporary hypoparathyroidism occured in the experimental group, and 3 cases occured in the control group, and there was no statistical sig-nificance difference between the two groups. Conclusion Carbon nanoparticles is benefit to the identification of parathyroid in surgery, and it can reduce the incidence of inadvertent parathyroidectomy, but it cann’ t reduce the incidence of hypoparathyroidism after procedure.

OBJECTIVE:To establish an HPLC method for the determination of the contents of Ligustrazine in rat's plasma,brain and liver.METHODS:Ligustrazine was separated on Hypersil ODS-C18 column with aspirin as internal standard.The mobile phase consisted of methanol-1.5% glacial acetic acid solution(45∶55,V/V)at a flow rate of 1.0 mL?min-1.The UV detection wavelength was 279 nm.RESULTS:The linear range of ligustrazine in rat's plasma,brain and liver was 0.006 25～7.813 ?g?mL-1,The lowest detectable limits were 0.5 ng?mL-1,1.55 ng?mL-1,and 1.55 ng?mL-1 and the average recoveries were 97.26%,96.44%,and 95.43% respectively with RSD at 3.40%,4.19% and 4.94%,respectively.CONCLUSION:With good linearity,precision and recovery,the method is sensitive and simple,and suitable for pharmacokinetic study and the research of Ligustrazine preparation.

Objective To investigate the expression of MMP-9 genes in primary hepatocellular carcinoma(HCC).Methods RT-PCR was used to detect MMP-9 mRNA in surgically extracted specimens from 28 patients with HCC.Results The expression of MMP-9 mRNA was significantly higher in tumor than in corresponding nontumoral tissue(P0.05).Meanwhile,the expression of MMP-9 mRNA in HCC was significantly correlated to the existence of portal vein tumor thrombus(P