Objective To clone rat NGB gene and prepare its polyclonal antibody in order to investigate the function of neuroglobin (NGB) Methods The total RNA was extracted from Wistar rat brain and the full length cDNA encoding NGB was obtained by RT-PCR Confirmed by sequencing analysis,it was inserted in the prokaryotic expression vector pET-28a(+) pET-28a(+)-NGB was expressed in Ecoli BL21 after its sequence and reading frame were confirmed by two restriction endonucleases and sequencing The protein of pET-28a(+)-NGB was used to immunize New Zealand rabbits for preparing polyclonal antibody after identification and purification The antibody titer was tested by ELISA method Results The titer of polyclonal antibody was 100 000 Immunoblot analysis showed the polyclonal antibody can recognize fusion protein expressed in 293 cells Conclusion The polyclonal antibody of NGB is prepared and can be used in further study

Objective To study and evaluate the effect of oxiracetam on brain injury. Methods Totally 60 patients with mild or medium brain injury were randomly assigned to receive oxiracetam or piracetam as control. MMSE and WMS scales were used to evaluate the therapeutic effects. Results The t value of MMSE and WMS was 15.85 and 5.97 in the oxiracetam group and 6.17 and 3.77 in the control group, with a significant difference between two groups (P

Residency standardized training is an important part to perfect the medical graduates' continuing medical education. It has an important and profound significance to train qualified medical talents, improve the clinical physician, and promote the whole team professional quality. It can improve residents' professional ability and quality generally through the residency standardized training of basic knowledge, basic skills, communication skills, professional ethics and so on.

OBJECTIVE To determine the bacterial distribution and resistance status in urinary system infections among old patients with primary susceptibility testing on urine samples. METHODS A total of 990 urine specimens were examined over 3 years to determine the validity of direct antimicrobial agent susceptibility testing.A total of 1149 strains collected from inpatient urine specimens during Oct 2002-Oct 2005 were identified and the drug resistance test was performed. RESULTS Escherichia coli rated the top one(57.9%),followed with Enterococcus,Proteus,Klebsiella pneumoniae,Pseudomonas aeruginosa,Staphylococcus and fungi.Enterobacteriaceae were showed increasing drug resistance trend,but still sensitive to imipenem(100%).G~+-cocci were also showed serious drug resistance trend,but still sensitive to vancomycin(100%)(except Enterococcus faecium). CONCLUSIONS Enterobacteriaceae are the main infectious bacteria in urinary system infection.Rational use of antibiotics should be carried out according to urine culture result.

OBJECTIVE To develop a prospective evaluation strategy to detect the first 3 days of incubation of the clinically significant isolates. METHODS With blood culture positive sample by BacT/Alert~3D and direct identification and antimicrobial susceptibility tests by VITEK32 in clinics to compare with those obtained from cards(GPI,GPS-106,GNI+,GNS-506) incubated with standardized bacterial suspension obtained following subculture to chocolate agar plates and CHRMagarTM microbiology. RESULTS There were 3 strategy reports performed.The sensitivity was 99.18%,specificity was 99.62%,validity was 98.89%,incidence rate was 25.4%,positive predictive value was 99.18%,negative predictive value was 99.62%,positive likelihood ratio was 261,and negative likelihood rate was 0.008. CONCLUSIONS In the first 3 days of incubation,99.36% of the(1 701) clinically significant isolates were detected,suggesting that routine incubation for more than 3 days be not necessary for FA and FN bottles.These findings suggest that VITEK-32 cards inoculated directly from BacT/Alert culture positive bottles provide acceptable bacterial identification or susceptibility testing for Enterobacteriaceae and Staphylococcus,and decrease the time lapse between initial inoculation of blood culture and the reporting of the results.

OBJECTIVE To study the distribution of the nonfermenter(NF) and the change in its resistance between 2002 and 2005 in our hospital ICU,and instruct clinical application of antibiotics reasonably.METHODS The(identification) of bacteria, antibiotic susceptibility and the detection by VITEK-32 of GNI~+、GNS-142 were used.Etest was applied for detection of metallo-?-lactamase.RESULTS The detective rate of the NF was 57.3%,(58.3%),67.5% and 69.8%,(respectively) from 2002 to 2005.Pseudomonas aeruginosa(PAE),Acinetobacter baumannii and (Stenotrophomonas) maltophilia accounted for 34.3%,11.1% and 4.0%,respectively.The highest distribution of the NF was in the sputum.The resistance rate of PAE to imipenem was lower,(however),it had rose from 0.0% to 17.8% in 2005.The resistance rate of S.maltophilia to sulfamethoxazole/(trimethoprim) was lower than the others,and rose from 0.0% in 2002 to 13.8% in 2005.The incidence rates of(metallo)-?-lactamase of PAE was 0.8% and of A.baumannii was 4.8%,respectively;the incidence rates of metallo-?-lactamase of A.baumannii and PAE were 0.8% and 4.8%,respectively.CONCLUSIONS The(detective) rates of PAE, and A.(baumannii) are gradually increased during the last four years.The incidence of(metallo)-?-lactamase of PAE and A.baumannii is also lower,and the other resistance is existed.

OBJECTIVE:To refine the quality standard of pharmacy intravenous admixture service(PIVAS),and provide refer-ence for improving the work quality of PIVAS. METHODS:Through establishing quality management organization and developing quality standard rules,quality control system for PIVAS in our hospital was constructed and total quality management was conduct-ed. Numbers of quality problems before(Jul. 2013-Jun. 2014)and after(Jul. 2014-Jun. 2015)its implementation were compared, and the effects were evaluated. RESULTS:117 management systems and 14 link quality standards and rules were made,including staff behavior standards,quality standards for drug management,supervision and inspection of quality standards,etc. Numbers of quality problems dropped from 358 to 177 after the implementation,the ratio of dispensing errors accounted for the total dispensing declined from 0.35? to 0.17?(P<0.05). CONCLUSIONS:The construction of quality control system and the implementation of quality control standards and rules in PIVAS of our hospital has improved the quality of PIVAS work.

Terapeutic options used to repair tendon and ligament injury consist of autografis,allografts and synthetic prostheses.Tendon and ligament of tissue engineering have their unique advantages as alternative therapy.Choosing the proper scaffold materials is important for successfully constructing tissue engineering tendon and ligament,so in this review we focus on natural and artificial scaffold materials used in tissue engineering of tendon and ligament in recent years.

Objective To construct prokaryotic cell expression vector of human Eotaxin mutants.Methods By point mutation,eight amino acid residues in the N-terminal(residues of 3-7)and N-loop(residue 14)regions of Eotaxin were individually mutated to methionine and residue 14 was delleted or methiomine was inserted after the residue 14,and then cloned respectively into prokaryotic cell expression vector-PET30a+.Results Eight N-terminal and N-loop mutants of human Eotaxin and their prokaryotic cell expression vector-PET30a+ were gained.Conclusion The successful construction of prokaryotic cell expression vector of human Eotaxin mutants lays a foundation for their expression and biological activity and for filtering antagonists of CCR3.

Objective To clone rat neuroglobulin (NGB) gene and construct its eukaryotic expression vector. Methods The total RNA was extracted from Wistar rat brain and the full length cDNA encoding NGB was obtained by RT-PCR. After the sequence was confirmed by sequencing and BLAST, it was inserted in the eukaryotic expression vector pCDNA3.1 (+), then the sequence and reading frame were confirmed by two restriction endonucleases and sequencing. Results The NGB gene was cloned with four bases mutated and its eukaryotic expression vector was constructed. Conclusion NGB expressed in Wistar rat brain. NGB gene was successfully cloned and inserted in eukaryotic expression vector.