The purpose of foreign language teaching in Chinese medicine is not only translation,but also clearly the role and the purposes of translation.Inputing a culture to another culture is not culture exchange,but cultural coagulation.Expressions with modem scientific system allow speculative natural philosophy freed from the ancient Chinese-style paper into the theory expressed by modem scientific language in order to solve the problems of teaching foreign languages of traditional Chinese medicine texts.

BACKGROUND: The endothelial-mesenchymal transition is known to play a central role in the pathological process of heterotopic ossification. OBJECTIVE: To verify the inhibitory effect of SMAD7 on endothelial-mesenchymal transition such as myofibroblast transformation and to explore whether it is a potential target for heterotopic ossification.METHODS: A lentivirus overexpressing SMAD7 was contructed and the optimal titre and transfection efficiency in rat aortic endothelial cells were determined. The lentivirus was then injected into a rat model of Achilles tendon injury, while the controls were given the injection of normal saline. Expressions of endothelial and mesenchymal markers at the injured site were analyzed by quantitative PCR and western blot assay. The heterotopic ossification was observed radiologically and histologically.RESULTS AND CONCLUSION: Local injection of SMAD7-delivering lentivirus resulted in an upregulation of CD31 and VE-cadherin, and a downregulation of N-cadherin and vimentin, suggesting that the endothelial-mesenchymal transition is blocked due to local SMAD7 overexpression. The inhibitory effect became more evident at 10 weeks than at 6 weeks after modeling. Radiology and histological staining further confirmed that the ossified structures in the tendon tissue disappeared after injection of SMAD7-delivering lentivirus, as opposed to the control group. These data suggest that local overexpression of SMAD7 can prevent postoperative heterotopic ossification with no effect on wound healing.

Objective To improve the current method of spermatogonial cells transplantation, and make the new method become more operative and easy to carry out. Methods The spermatogonial cells in rC57BL6/tg14 (act-EGFO-Osb Y01) mice that expresse GFP protein were collected as the donor cells, and by using a modified syringe, and then were injected into the seminiferous tubules of pretreated wild type GFP-null mice through testicular efferent duct. The transplantation outcome was evaluated by trypan blue stainting and fluorescent microscopic examination and pathohistological analysis of transplanted testis. Results The transplanted testis of recipient mice showed green fluorescence signal, and the signal of seminiferous tubules was found significantly higher than that of the surrounding tissues. The transplanted GFP-positive cells generated colonies and spermatogenesis but pretreated wild type GFP-null mice were not found. Conclusion The expressed GFP protein spermatogonial cells in rC57BL6/tg14 mice were successfully transplanted in the wild type GFP-null recipient mice, fourthermore the improved transplantation method simplified the triditional one and achieved the same transplantation results.

Objective To research the reduction of computed radiography exposure.Methods By body-model computed radiography test,the relationship between X-ray exposure on the image plate to the latitude and sensitivity of the exposure data recognizer was investigated,from which the low-dose value of exposure was achieved.Results The low-dose value of the computed radiography was 22.5% of the average-dose.Conclusion The low-dose of computed radiography can be achieved.

Objective To investigate the CT and MRI findings of facial neuroma to promote diagnostic accuracy. Methods Nine facial neuromas consisted of 8 schwannoma and 1 neurofibroma. High resolution CT were performed on 7 cases, routinal CT scan on 2 patients. Six cases were examined by MRI, in which 5 cases were used constrast media. Results Five tumors on right side and 4 on left were detected. The tumor affected the internal auditory canal segment of facial nerve in 1 case, labyrinth segment in 4, anterior genu in 6, tympanum segment in 7, mastoid segment in 6,horizontal segment in 5 and parotid segment in 3. CT demonstrated enlargement of the facial nerve canal, benign bone remodeling in the location of geniculate ganglion and stripe soft issue along the course of facial nerve. MRI found that involved facial nerve became thickened markedly as a stripe or soft mass by several segments, that were isointense to brain on T 1WI and isointense or hyperintense on T 2WI and enhanced obviously. Conclusion The segmental soft tissue mass along the facial nerve course is the characteristic of facial neuroma. Gadolinium enhanced MRI is an effective method for detecting the lesion.

PurposeTo explore the value of prenatal ultrasound diagnosis of pentalogy of Cantrell.Materials and Methods A retrospective study was performed in 6 fetuses with pentalogy of Cantrell diagnosed with prenatal ultrasound. The first diagnostic ultrasound time, ultrasound images and follow-up results were reviewed to analyze ultrasonographic features.Results All the fetuses were characterized by omphalocele and ectopic heart. All 6 fetuses were diagnosed by prenatal ultrasound during the first ultrasound screening, of which 5 were diagnosed before 16 weeks of gestation. Five cases were confirmed with induced labor and 1 was confirmed postnataly. The accuracy of prenatal ultrasonic diagnosis was 100%.Conclusion The characteristic features of pentalogy of Cantrell are omphalocele and ectopic heart. Prenatal ultrasound is reliable and valuable to diagnose pentalogy of Cantrell.

The patient doctor relationship,as the essential part of medical relationship, is inevitably influenced by the medical views and develops with the evolvement of medical modes. Understanding the inner logical relationships between the medical mode and the patient-doctor relationship can facilitate the further research on the patient-doctor relationship. Currently, we should choose the mode of the patient-doctor relationship on the basis of respecting patient, emphasizing the obligation, motivating the activity of patients, and constructing an honesty-trust relationship between patient and doctor, which will make it easy to understand the ethical request of the constructing of patient-doctor relationship and make the definition of the patient doctor relationship clearer.

Objective To screen a thermostable urate oxidase-producing strain, optimize the fermentation conditions and study the enzymatic properties.Methods A urate oxidase-producing strain was screened from high temperature starter based on transparent circle method.Its 16S rDNA sequence was then amplified and analyzed.Meanwhile, the phylogenetic trees were built.Optimization of the fermentation conditions from this strain was carried out.The enzymatic properties of urate oxidase were studied.Results A urate oxidase-producing strain, named Bacillus subtilis ZX-6 by molecular identification, was obtained.The production of urate oxidase under the optimized conditons (135.9 U/L) was 133.7%higher than before.The optimum reaction temperature and pH were 45℃ and 7.6 respectively.The residual activity of urate oxidase at 37℃ for 48 h was still 17.2%.Conclusion The successful screening of a thermostable urate oxidase-producing strain and optimization of the fermentation conditions will lay a foundation for the further research.

Cluster Analysis ; Fuzzy Logic ; Humans ; Mathematics ; Medicine, Chinese Traditional

Objective To investigate the relationship between rs7903146-T/C polymorphism of transcription factor7-like 2 gene (TCF7L2) gene and gestational diabetes mellitus in Han population of Liaoning province.Methods The rs7903146-T/C polymorphism was detected in 115 healthy Han controls and 113 patients with gestational diabetes mellitus by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.Results There were three genotypes:CC,CT,and TT; and their frequencies in control group and patients were 95.65％/84.96％,4.35％/15.04％,and 0/0,respectively.The allele frequency T of rs7903146 in control group and the patients was 2.17％ and 7.52％,respectively.There was statistical difference between two groups.Conclusions The rs7903146-T/C polymorphism of TCF7L2 might be associated with gestational diabetes mellitus in Liaoning Han population.