1.Assessment of myocardial viability with dobutamine stress echocardiography versus ~(99m)Tc single photon emission computed tomography
Xiaoping JI ; Wenqiang CHEN ; Yun ZHANG
Chinese Journal of Ultrasonography 2003;0(07):-
Objective To assess the value of dobutamine stress echocardiography (DSE) in predicting myocardial viability by comparing with single photon emission computed tomography(SPECT).Methods Taking improvement of ventricular wall motion as a standard,fifteen patients undergone percutaneous coronary artery intervention(PCI) were detected by DSE and SPECT respectively,comparing their accuracy in predicting myocardial viability.Results By DSE, difference of the sensitivity and negative forecasting value was remarkable between peak dose stage and low dose stage,comparing with DSE,SPECT had the highest sensitivity and the lowest distinctivity.Conclusions The two techniques have their own advantages and disadvantages in predicting myocardial viability,but DSE is more practical.
2.Construction and identification of recombinant lentivirus vector for microRNA-223 overexpression and suppression.
Yun WANG ; Ning JI ; Min ZHOU ; Lu JIANG ; Qianming CHEN
West China Journal of Stomatology 2015;33(5):451-455
OBJECTIVETo construct microRNA-223 overexpression and suppression lentivirus vectors and determine their effects after infecting oral squamous cell carcinoma (OSCC) cell line.
METHODSLentivirus vectors GV229 and GV232 were cut by the restriction sites of Age I and EcoR I and connected to the target gene, which contained mature microRNA-223 and microRNA-223 oligonucleotide. Real-time polymerase chain reaction (PCR) method was used to detect the microRNA-223 expression level after infecting the recombinant lentivirus vector into the OSCC cell line.
RESULTSThe successful construction of microRNA-223 recombinant lentivirus vectors was confirmed by the PCR method and DNA sequencing. HN-30 cell infected with microRNA-223 overexpression vector showed a significant increased in microRNA-223 expression, whereas HN-30 cell infected with microRNA-223 inhibitor vector suppressed microRNA-223 expression.
CONCLUSIONThe microRNA-223 overexpression and suppression lentivirus vectors are successfully constructed. These vectors could alter the expression level of microRNA-223 in OSCC cell line significantly, and provide a stable cell line for functional studies in the future.
Base Sequence ; Cell Line, Tumor ; Genetic Vectors ; Humans ; Lentivirus ; MicroRNAs ; metabolism ; Real-Time Polymerase Chain Reaction
3.Clinial observation of cognitive disorder complicated with aphasia after brain injuries
Wei CHEN ; Xiaoping YUN ; Huazhen GUO ; Shurong JI
Chinese Journal of Rehabilitation Theory and Practice 2001;7(2):82-83
ObjectiveTo determine whether aphasia has effect on the degree of cognitive disorder for the patients with brain injury, so that prognosis can be determined. MethodsThere are 35 cases of patients with brain injury and they are divided into two groups by aphasia screening test with aphasia and without aphasia. Those with cognitial capacity screening examination(CCSE) and the Loewenstein Occupational Therapy Cognitive Assessment Battery(LOTCA) are examined. ResultThe result is that there is significant differences between these two groups of patents. ConclusionThe patients with brain injury who complecated with aphasia had worse cognitive disorder.
4.A comparative study of cytology,histopathology and immunohistochemistry of malignant lymphoma of lung mucosa-associated lymphoid tissues
Hongai JI ; Jinghua WANG ; Xiaojun ZHOU ; Yun JI ; Jie MA ; Qunli SHI ; Jieyu CHEN
Journal of Medical Postgraduates 2004;0(01):-
Objective: To explore the cytopathologic features and differential diagnostic essentials of the lung mucosa-associated lymphoid tumor(MALT).Methods: The fine needle aspiration(FNA) tissues and bronchi smears of 4 cases of MALT were examined by cytology,histology and immunohistochemistry respectively.Results: Cytologically,3 of the 4 cases were definitely diagnosed as lymphoid tumor and 1 suspected of small cell undifferentiated carcinoma,while histopathologically,all were diagnosed as MALT.The neoplastic cells were positive for CD20 and CD79a,but negative for CD3,CD5,CD10,CD45RO,CKpan and EMA.Conclusion: MALT is a rare pulmonary lymphoma difficult to be diagnosed.The definite diagnosis of pulmonary MALT depends on the cytology of FNA and bronchi smears,histopathology,and immunohistochemistry.
5.Experimental study on induction of atherosclerotic plaque instability in rabbits after transfer of wild-type p53 gene
Wenqiang CHEN ; Yun ZHANG ; Mei ZHANG ; Xiaoping JI ; Chen LIN ; Yongfeng ZHU ; Xuedong SANG
Chinese Journal of Pathophysiology 1989;0(05):-
AIM: To study the apoptotic role of wild-type p53 in induction of plaque instability in atherosclerotic rabbits. METHODS: Fifty-four New Zealand White rabbits underwent balloon-induced abdominal aortic wall injury and then were fed on a diet of 1% cholesterol. At the end of the eighth week, the rabbits were randomly divided into two groups: group A and group B. Recombinant adenovirus carrying p53 and ?-galactosidase (LacZ) genes were injected in group A and B, respectively. Two weeks later, 10 rabbits each in group A and B was killed and the remaining rabbits all underwent pharmacological triggering with injection of Chinese Russell's viper venom and histamine. RESULTS: Compared with group B, p53 gene over-expression in group A resulted in a marked increase in number of positive apoptotic cells (2.5%?0.8% vs 1.0%?0.3%, P
6.Expression of transforming growth factor-? and metalloproteinases in joint capsule of frozen shoulder
hong-yun, LI ; shi-yi, CHEN ; wei-tao, ZHAI ; ji-wu, CHEN
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(11):-
Objective To explore the role of transforming growth factor (TFG)-? and matrix metalloproteinases(MMPs)in the development of frozen shoulder. Methods Twenty-four patients who underwent shoulder arthroscopy were included,and were divided into frozen shoulder group ( n =12) and control group ( n =12; n =2 for shoulder instability,n =5 for rotator cuff tear and n =5 for subacromial impingement). Joint capsule tissues at the rotator cuff interval were obtained,and the expression of TGF-?,MMP-1,MMP-2,MMP-3,MMP-9 and MMP-12 mRNA and protein was detected by Real-time PCR and Western blotting,respectively. Results The expression of TGF-? mRNA in frozen shoulder group and control group was 3.36?10 4?2.18?10 3 and 1.85?10 4?3.31?10 3,respectively,the expression of TGF-? protein was 1.55?0.33 and 1.13?0.21,respectively,and there were significant differences between these two groups ( P
8.Evaluation of biatrial function after rapid right atrial pacing in a canine model by acoustic quantification technique
Lei ZHANG ; Xiaoping JI ; Wei ZHANG ; Rong WANG ; Shiliang JIANG ; Wenqiang CHEN ; Xuedong SANG ; Yun ZHANG
Chinese Journal of Ultrasonography 2003;0(11):-
Objective To observe short-term rapid atrial electrical activation on atrial size and atrial systolic function. Methods Twelve anesthetized mongrel dogs were underwent insertion of a transvenous lead at the right atrial appendage and subjected to 5 hours of rapid right atrial pacing at 450 beat/min. Acoustic quantification(AQ) waveform on biatrial was recorded before and after 5 hours pacing with AQ technique. All measurements were made in sinus rhythm. Results After 5 hours rapid right atrial pacing, end systolic volume(ESV) of both left and right atrial enlarged significantly from ( 5.92 ? 1.51 )ml to ( 7.27 ? 1.86 )ml,from ( 3.13? 0.71 )ml to ( 3.58 ? 0.73 )ml, respectively; but biatrial function decreased markedly,left and right atrial ejection fraction decreased from ( 51.03 ? 9.45 ) to ( 38.23 ? 10.52 ),from ( 36.76 ? 5.36 ) to ( 30.71 ? 7.97 ), respectively. However, left atrial ESV enlarged more than right atrial (by 23 vs 8,P
9.Effects of TiO2 nanoparticles on antioxidant function and element content of liver and kidney tissues in young and adult rats
Yun WANG ; Zhangjian CHEN ; Te BA ; Ji PU ; Xiaoxing CUI ; Guang JIA
Journal of Peking University(Health Sciences) 2014;(3):395-399
Objective:To compare the effect of TiO 2 nanoparticles on antioxidant function and element content of liver and kidney tissues in young and adult rats .Methods:Forty-eight SD male rats , half in 4-week (youth) old and half in 9-week (adult) old rats, were randomly divided into 8 groups, which were exposed to TiO2 nanoparticles [(75 ±15) nm, anatase] through intragastric administration at 0, 10, 50 and 200 mg/kg body weight every day for 30 days.The liver and kidney tissues were collected for antioxidant function and element content analysis .Results: 200 mg/kg TiO2 nanoparticles exposure significantly increased the liver total superoxide dismutase ( T-SOD ) activity and the kidney reduced glutathione (GSH)/oxidized glutathione (GSSG) ratios in young rats, and significantly decreased the liver Mo, Co, Mn and P contents and the kidney Rb and Na contents in young rats .200 mg/kg TiO2 nanoparticles exposure significantly increased GSH/GSSG ratios and Rb contents and decreased Na con-tents in the liver of adult rats .No significantly difference was found in antioxidant indexes and elements content in the kidney of adult rats between three experimental groups and control group .Conclusion:TiO2 nanoparticles can enhance the antioxidant capacity and decrease the elements content in rat liver and kidney tissues .The liver is the more sensitive target organ and the young animals are more susceptible to TiO2 nanoparticles toxicity by the oral routes .
10.Simultaneous determination of five constituents in Jiangzhi Granules by HPLC
Yun MA ; Qiwen WANG ; Guohua XU ; Lejun JI ; Jianwei CHEN ; Xiang LI
Chinese Traditional Patent Medicine 2017;39(3):532-535
AIM To establish an HPLC method for the simultaneous content determination of five constituents in Jiangzhi Granules (Salviae miltiorrhizae Radix et Rhizoma,Polygoni cuspidati Rhizoma et Radix,Artemisiae scopariae Herba,etc.).METHODS The analysis of methanol extract of this drug was performed on a 30 ℃ thermostatic Hanbon-C18 column (4.6 mm × 250 mm,5 μm),with the mobile phase comprising of acetonitrile (A) 0.1% phosphoric acid (B) flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 286 nm (0-30,40-50 min) and 203 nm (30-40 min).RESULTS Gypenoside A,chlorogenic acid,polydatin,salvianolic acid B and emodin showed good linear relationships within the ranges of 0.176-2.46,0.056-0.84,0.36-5.40,0.192-2.88 and 0.12-1.80 μg (r >0.999 0),respectively,whose average recoveries were 97.17%-102.59% with the RSDs of 1.9%-2.9%.CONCLUSION This accurate and simple method can be used for the quality control of Jiangzhi Granules.