We attempted to identify parasite DNA in the biliary stones of humans via PCR and DNA sequencing. Genomic DNA was isolated from each of 15 common bile duct (CBD) stones and 5 gallbladder (GB) stones. The patients who had the CBD stones suffered from cholangitis, and the patients with GB stones showed acute cholecystitis, respectively. The 28S and 18S rDNA genes were amplified successfully from 3 and/or 1 common bile duct stone samples, and then cloned and sequenced. The 28S and 18S rDNA sequences were highly conserved among isolates. Identity of the obtained 28S D1 rDNA with that of Clonorchis sinensis was higher than 97.6%, and identity of the 18S rDNA with that of other Ascarididae was 97.9%. Almost no intra-specific variations were detected in the 28S and 18S rDNA with the exception of a few nucleotide variations, i.e., substitution and deletion. These findings suggest that C. sinensis and Ascaris lumbricoides may be related with the biliary stone formation and development.
Aged ; Aged, 80 and over ; Animals ; Ascaridida/genetics/isolation & purification ; Ascaris lumbricoides/genetics/isolation & purification ; Base Sequence ; Clonorchis sinensis/genetics/isolation & purification ; Common Bile Duct/*parasitology ; DNA, Helminth/*genetics ; DNA, Ribosomal/genetics ; Face/parasitology ; Female ; Gallbladder/parasitology ; Gallstones/*parasitology ; Helminths/genetics/*isolation & purification ; Humans ; Male ; Middle Aged ; Molecular Sequence Data ; Polymerase Chain Reaction/*methods ; RNA, Ribosomal, 18S/genetics ; RNA, Ribosomal, 28S/genetics ; Sequence Alignment

No abstract available.
Carcinoma in Situ* ; Cervix Uteri* ; Female

PURPOSE: This study was done to explore the relationship between aggression and internet over-use, depression-anxiety, self-esteem, all of which are known to be behavior and psychological characteristics linked to "at-risk" children for aggression. METHODS: Korean-Child Behavior Check List (K-CBCL), Korean-Internet Addiction Self-Test Scale, and Self-Esteem Scale by Rosenberg (1965) were used as measurement tools with a sample of 743, 5th-6th grade students from 3 elementary schools in Jecheon city. Chi-square, t-test, ANOVA, Pearson's correlation and stepwise multiple regression with SPSS/Win 13.0 version were used to analyze the collected data. RESULTS: Aggression for the elementary school students was positively correlated with internet over-use and depression-anxiety, whereas self-esteem was negatively correlated with aggression. Stepwise multiple regression analysis showed that 68.4% of the variance for aggression was significantly accounted for by internet over-use, depression-anxiety, and self-esteem. The most significant factor influencing aggression was depression-anxiety. CONCLUSION: These results suggest that earlier screening and intervention programs for depression-anxiety and internet over-use for elementary student will be helpful in preventing aggression.
*Aggression ; Anxiety ; Behavior, Addictive ; Child ; Child Behavior/psychology ; Depression ; Female ; Humans ; Internet ; Male ; Self Concept ; Students/*psychology

OBJECTIVES: This study investigates the relationship between risky health behaviors, occurrence of oral symptoms, and oral health-related behaviors. METHODS: The subjects were 60,040 adolescents selected from the web-based survey from the 2018 Korean Youth Health Behavior of Korean Center for Disease Control. The data were analyzed using a combined sample analysis method. The software SPSS version 12.0 was used to conduct the analyses. RESULTS: 1. Men were observed to partake in risky health behavior at significantly higher rates than were women in terms of smoking, drinking, drugs, and sexual intercourse. Our data showed the percentages of men taking part in these behaviors to be 21.1%, 46.4%, 1.3%, and 7.6%, respectively (P<0.01). 2. Of all subjects, 49.1% brushed their teeth more than three times a day, and 43.6% brushed more than twice a day. Further, a significantly higher number of men (46.0%) did not brush their teeth after lunch compared with women (29.1%) (P<0.01). 3. Of all subjects, 52.9% had developed oral symptoms in the previous year. The rate of women experiencing oral symptoms was significantly higher, at 58.1%, compared with men (48.1%, P<0.01). 4. Risky health behavior, such as smoking, drinking, drugs, and sexual intercourse, was significantly associated with occurrence of oral symptoms (P<0.01). Those that had not partaken in smoking, drinking, and sexual intercourse were significantly lower by 0.73, 0.87, and 0.89 times, respectively, compared with those that responded with “yes” (P<0.01). CONCLUSIONS: We have evaluated the rates of risky health and oral health-related behavior in Korean youth and identify their association with the oral symptoms. It is essential to understand the risks of oral diseases so that appropriate oral health education can be provided to adolescents for promoting behavioral changes.
Adolescent ; Centers for Disease Control and Prevention (U.S.) ; Coitus ; Drinking ; Education ; Female ; Health Behavior ; Humans ; Korea ; Lunch ; Male ; Methods ; Oral Health ; Risk-Taking ; Smoke ; Smoking ; Tooth

Objectives: This study aimed to investigate the relationship between toothache, oral health behaviors, and the quality of health-related life of Korean adults. A national nutrition survey representative of Korea was used. The study presents basic material for developing health improvement and dental health programs to highlight the importance of oral health management and Health-Related Quality of Life improvement. Methods: The second edition of the 8th national health nutrition survey data was used for this study. The subjects were 5,922 Korean adults aged 19 years and above. Results: There was a significant difference in gender, age, marital status, education, income, toothache experience, subjective oral health condition, and the number of daily toothbrushes in participants’ Health-Related Quality of Life. For gender, “male” had an overall higher quality of life which was statistically significant. In toothache experience, Health-Related Quality of Life was significantly higher at 0.027 with “none” (P<0.01). In addition, higher conditions of subjective oral health at 0.077 showed a significantly higher quality of life (P<0.01). Conclusions This study revealed that higher subjective oral health conditions in Korean adults resulted in higher Health-Related Quality of Life and subjectively better oral health conditions. This means that active health management is possible, and concrete systematic health education program development is urgently needed. Oral health improvement can improve Health-Related Quality of Life. It is necessary to recognize the importance of oral health to improve Health-Related Quality of Life through oral health prevention and promotion programs for adults.

Objectives: This study used data from the seventh National Health and Nutrition Survey, a dataset which signifies that dental services in the country exhibit relatively low health insurance coverage and high copay rates compared to other medical healthcare services. We surveyed the utilization rate of dental care and the prevalence of untreated conditions among Korean elders aged over 65.Furthermore, we aimed to present policy implications to improve dental care accessibility and expand health insurance coverage for elders, especially vulnerable individuals who are bedridden or living alone. Methods: We used raw data from the seventh National Health and Nutrition Survey to investigate Korean elders’ dental service utilization and untreated conditions. We opted for elders aged over 65 and finalized 1,712 subjects for the analysis. Statistical analysis was performed using IBM SPSS 25.0 (IBM Corp., Chicago, IL, USA). Groups were detailed in terms of strata of variation estimation and elders aged over 65, cluster of district enumeration, and weighted oral survey. We generated a scheme file and employed complex sampling analysis with a statistical significance level of P<0.05. Results: This study intended to survey the rate of dental care use and untreated care of Korean elders over 65 using the seventh National Health and Nutrition Survey data which represents our country. 1. Dental care use rate based on general traits showed statistically significant relevance (P<0.01) with lower age, higher income, higher education level, residence of ‘Dong’, and ‘with’ private insurance. 2. In untreated rate of dental care by general traits showed the significant total of 26.1% with female, lower income, lower education level, ‘recipient’ of basic living, and subjectively poor status of oral health. 3. The multiple answers of the patients who went to the dentists over the past year showed dental care details of dental checkups 54.3%, prosthetic dentistry 32.1%, preventive treatment 30.9%, cavity of root canal treatment 25.7%, gum treatment 17.1%, and tooth extraction 14.7%. 4. The reasons for untreated dental care of those untreated subjects showed the highest 34.0% of economic reason, mild condition 32.3%, lack of time 8.2%, and scared of treatment 8.0% in order. Based on this analysis, we intended to suggest policy implications for the necessity of dental checkup expansion and the activation of prevention treatment. Conclusions To improve dental care accessibility for elders aged 65 and above, especially the vulnerable individuals who are bedridden or living alone, it is imperative to expand outpatient insurance coverage and tailor the dental services as per their specific needs. Shifting the focus from treatment-oriented health insurance to preventive measures and examination service expansion and invigoration is essential to improve dental health and overall quality of life. It is deduced that expansion of dental care health insurance coverage and checkup services is critical for vulnerable elders, such as those who are bedridden or living alone.

PURPOSE: Bone morphogenetic proteins (BMPs) are members of the transforming growth factor-beta family and play an important role in cellular growth. Recent reports suggest that exogenous bone morphogenetic protein-2 (BMP-2) acts as an antiproliferative agent in a variety of cell lines. We will study whether BMP-2 is altered in human colorectal cancer. METHODS: We analyzed 40 colorectal cancer cases and 6 colorectal cancer cell lines by using reverse transcription-polymerase chain reaction (RT-PCR) to determine the expression of BMP-2. RESULTS: Thirteen of 40 colorectal cancers (33%) and 3 of 6 colorectal cancer cell lines (50%) revealed decreased expression of BMP-2. The rates of decreased expression were 0% (0/7), 42.1% (8/19), 28.6% (2/7), 33.3% (2/6), and 100% (1/1) in stages I, II, III, and IV, respectively. Histologically, the rates were 33.3% (2/6), 32.2% (10/21), 50% (1/2), and 0% (0/1) in well-differentiated, moderately-differentiated, poorly-differentiated and mucinous cancers, respectively. As for location, the rates for colon and rectal cancers were 27.8% (5/18) and 36.4% (8/22), respectively. We identified methylation in the CpG island of the BMP-2 gene in 60% of colorectal cancer cells and in 50% of colorectal cancer cell lines. The 13 cases without BMP-2 gene expression showed no significant correlation with clinicopathological factors. Epigenetic silencing through DNA methylation is one of the key steps during carcinogenesis. CONCLUSION: We found, through an analysis using the methylation-specific polymerase chain reaction technique, CpG island methylation of the BMP-2 promoter region in colorectal cancer. Thus, aberrant BMP-2 methylation and the resultant loss of BMP-2 expression may be related to colorectal carcinogenesis.
Bone Morphogenetic Proteins ; Cell Line ; Colon ; Colorectal Neoplasms ; CpG Islands ; DNA Methylation ; Epigenomics ; Gene Expression ; Humans ; Methylation ; Mucins ; Polymerase Chain Reaction ; Promoter Regions, Genetic ; Rectal Neoplasms

To investigate the risk factors and clinical characteristics of postrenal transplant diabetes mellitus (PTDM), we reviewed the records of 177 renal allograft recipients in Maryknoll Hospiatal whose allografts had functioned longer than 6 months. Nineteen patients (10.7%) developed PTDM at 5.0+/-7.8 (1-52) months; 9 (47%) of these within 1 month. PTDM patients were older than nondiabetic renal transplants (42+/-2 vs 37+/-1 years, P<0.05). Body mass index tended to be higher in PTDM (23.5+/-1.0 vs 21.8+/-0.3kg/m2, P=0.09). Number of acute rejections (0.6+/-0.2 vs 0.5+/-0.1) and serum creatinine at 1 year after transplantation (1.2+/-0.8 vs 1.3+/-0.3mg/dL) were not different. Fasting (103.6+/-10.4 vs 84.4+/-1.6mg/dL, P<0.05) and postprandial (189.2+/-24.8 vs 118.6+/-2.3 mg/dL, P<0.01) blood sugars, measured before transplantation, were higher in PTDM. CsA blood level at 1 month posttransplantation was higher in PTDM (350+/-34 vs 279+/-8ng/mL, P<0.05). Fasting serum insulin was significantly higher (28.2+/-12.2 vs 7.3+/-2.0 microunit/dL, P<0.05) and serum C-peptide tended to be higher in PTDM patients compared with euglycemic renal recipients (6.3+/-1.6 vs 3.8+/-0.9ng/dL, P=0.08). All the PTDM patients were treated by either insulin or oral agent; 15 of 19 required no treatment after 4.7+/-6.9 months. In conclusion, prevalence of PTDM was 10.7%. PTDM patients were older. Body mass index was tended to be higher. Fasting and postprandial blood sugars, measured before transplantation, were higher in PTDM. Faslting serum insulin was higher and C-peptide tended to be higher in diabetics. These results suggested that increased insulin resistance plays a major role in the pathogenesis of PTDM.
Allografts ; Blood Glucose ; Body Mass Index ; C-Peptide ; Creatinine ; Cyclosporine ; Diabetes Mellitus* ; Fasting ; Humans ; Insulin ; Insulin Resistance ; Prevalence ; Risk Factors*

BACKGROUND: Health illiteracy is a problem often unrecognized by health care providers. It influences medical costs and the health status of adults. The purposes of this study were to determine the level of health literacy in community-dwelling adults and to identify the factors influencing it. METHODS: A cross-sectional survey was conducted in Seoul, Gyeonggi and Chungcheong province. A total of 420 adults aged 18 or older were interviewed by trained nursing students between November 1 to December 30, 2011. Health literacy was measured using the Short Test of Functional Health Literacy in Adults. Data were analyzed using the PASW 18.0 program. RESULTS: The mean score of health literacy was 50.64+/-19.18. In the multiple linear regression analysis, health literacy was significantly associated with education (beta=0.17, P=0.001), alcohol use (beta=-0.12, P=0.010), and perceived health status (beta=0.11, P=0.029). These factors accounted for about 7% of health literacy. CONCLUSIONS: Health literacy is a very important public health issue. Our findings showed that educational level, alcohol use and perceived health status should be considered when assessing this issue in patients. Furthermore, the development of a standardized Korean assessment tool for health literacy and specified interventions for enhancing health literacy are needed to improve health outcomes.
Adult* ; Cross-Sectional Studies ; Education ; Gyeonggi-do ; Health Literacy* ; Health Personnel ; Health Status ; Humans ; Linear Models ; Literacy ; Primary Prevention ; Public Health ; Seoul ; Students, Nursing

A 44-year-old man presented with a history of chronic epiphora, discharge from the right eye, and a palpable mass in the medial canthal area. Irrigation of the lacrimal system revealed bloody discharge. Orbital magnetic resonance imaging (MRI) showed a well-defined heterogeneous enhanced mass filling the lacrimal sac and upper nasolacrimal duct (NLD). A wide excision and surgical biopsy were performed. Histopathology showed the tumor to be an exophytic Schneiderian papilloma with moderate to severe dysplasia. Three months later, the mass was found to be invading the nasal cavity through the NLD. Endoscopic histopathological evaluation confirmed that it was identical to the originally identified papilloma.
Adult ; Biopsy ; Diagnosis, Differential ; Endoscopy ; Eye Neoplasms/*pathology/surgery ; Follow-Up Studies ; Humans ; Lacrimal Apparatus/*pathology ; Magnetic Resonance Imaging ; Male ; Nasal Mucosa/*pathology ; Neoplasm Invasiveness/*pathology ; Neoplasm Recurrence, Local/*pathology/surgery ; Nose Neoplasms/*pathology/surgery ; Papilloma/*pathology/surgery