1.Baicalein enhances the gap junction in the TM4 Sertoli cells of mice.
Guo-jun JIANG ; Shu-ying DONG ; Jie JI ; Hao RU ; Xu-hui TONG
National Journal of Andrology 2015;21(8):687-691
OBJECTIVETo investigate the effect of baicalein on the gap junction intercellular communication (GJIC) in the TM4 Sertoli cells of the mouse testis and its related mechanism.
METHODSWe measured the cytotoxicity of different concentrations of baicalein on the TM4 Sertoli cells in the mouse testis by MTT, detected the fluorescence transfer of the TM4 Sertoli cells by parachute assay, and determined the expression of the protein connexin 43 ( Cx43) in the baicalein-treated cells by Western blot and immunofluorescence assay.
RESULTSBaicalein produced no obvious cytotoxicity on the TM4 Sertoli cells at the concentration below 60 µmol/L but significantly increased their GJIC at 0-20 µmol/L (P < 0.01). Western blot and immunofluorescence assay showed that 0-20 µmol/L baicalein remarkably elevated the expression of Cx43 in the TM4 cells (P < 0.01) and on the membrane of the TM4 cells.
CONCLUSIONBaicalein at the concentration of 0-20 µmol/L can significantly enhance GJIC in mouse TM4 Sertoli cells by increasing the expression of the Cx43 protein.
Animals ; Cell Communication ; drug effects ; Connexin 43 ; metabolism ; Flavanones ; administration & dosage ; pharmacology ; Gap Junctions ; drug effects ; Male ; Mice ; Sertoli Cells ; drug effects ; metabolism ; ultrastructure
2.Quantitative analysis of the image quality in megavoltage cone-beam computed tomography
Weigang RU ; Zhen ZHANG ; Xi CHANG ; Ji ZHU ; Zhiyong XU ; Jintian TANG ; Guoliang JIANG
Chinese Journal of Radiation Oncology 2009;18(3):226-229
Objective To quantitatively analyze the image quality of megavoltage cone-beam CT (MVCBCT) under different scanning conditions to provide reference in clinical applications. Methods Si-emens ONCOR linear accelerator with MVCBCT was used to scan the phantom under different conditions. The image quality was evaluated in terms of image noise, uniformity, spatial resolution, contrast resolution, the number of Monitor Units(MUs) used in imaging,and the size of the reconstruction matrix. The comparison of the image quality between MVCBCT and conventional simulator CT was also analyzed. Results The image noise was decreased with the increase of the number of MUs. The uniformity index showed that the system u-niformity was weakly dependent on MU numbers or the size of the reconstruction matrix. Except for the ima-ges with 5 MUs,all other images had the spatial resolution of 0.4 lp/mm with a reconstruction matrix of 256 ×256. Better low contrast resolution was achieved by using more MUs. For typical pelvis and head-and-neck patients,the imaging dose at the center was 0.8 cGy/MU and 0.7 cGy/MU, respectively,and the maxi-mum dose was about 1.2 cGy/MU. For typical abdomen patients,the image maximum dose and center dose was 1.3 cGy/MU and 0.7 cGy/MU,respectively. Conclusions The image quality of MVCBCT is inferior to the conventional kilo-voltage CT. However,with the optimization of the parameters in imaging,we can a-chieve sufficient image contrast in the bone,air and some soft-tissue structures with low imaging dose to pa-tients. Such images can be used for IGRT.
3.Risk factors of nosocomial infections in patients with head and neck cancer during radiotherapy
Haibing ZHANG ; Jianwei XU ; Shaojun ZHENG ; Lixin RU ; Genhua YU ; Pengtian JI
Chinese Journal of Clinical Infectious Diseases 2016;(1):32-36
Objective To investigate the risk factors of nosocomial infections in patients with head and neck cancer during radiotherapy .Methods Clinical data of 189 patients with head and neck cancer receiving radiotherapy in Huzhou Central Hospital during August 2011 and December 2014 were retrospectively studied .Chi-square test and multivariate Logistic regression analysis were performed to explore the risk factors of nosocomial infections in patients during radiotherapy .Results Among 189 patients with head and neck cancer receiving radiotherapy , nosocomial infection was observed in 64 (33.86%) patients.Univariate analysis showed that radiation-induced oral mucositis (χ2 =11.47, P<0.01), underlying disease (χ2 =6.59, P<0.05), stage of tumor Ⅲ-Ⅳ (χ2 =4.36, P<0.05), whole-neck radiation (χ2 =7.64, P <0.05), dose of radiation ≥50 Gy(χ2 =8.92, P <0.05), combined chemotherapy (χ2 =6.53, P<0.01), invasive operation (χ2 =4.21, P<0.05), PS score=1 (χ2 =3.58, P<0.05) and low body mass index (χ2 =9.56, P <0.05) were risk factors of nosocomial infections . Multivariate Logistic regression analysis indicated that radiation-induced oral mucositis ( OR=3.46, χ2 =8.72, P<0.01),underlying disease (OR=1.91, χ2 =4.66, P<0.05), stage of tumor Ⅲ-Ⅳ (OR=2.01,χ2 =5.22, P<0.05), whole-neck radiation (OR=1.78,χ2 =4.89, P<0.05), dose of radiation ≥50 Gy (OR=1.69,χ2 =3.25, P<0.05), combined chemotherapy (OR=1.84, χ2 =6.91, P<0.05) and low body mass index (OR=1.53, χ2 =2.59, P<0.05) were independent risk factors of nosocomial infection.Conclusions Nosocomial infections are popular in patients with head and neck cancer during radiotherapy.Radiation-induced oral mucositis, underlying disease, stage of tumor, radiation field, dose of radiation, combined chemotherapy and body mass index are associated with the occurrence of nosocomial infections in these patients .
4.In vitro inhibition of trichosanthin-monoclonal antibody conjugate on human melanoma cells
Ru-Ping ZHANG ; Chi-Jie XU ; Hui-Ting CAO ; Rui-Hua JI ; Zu-Chuan ZHANG ;
Chinese Journal of Immunology 1985;0(06):-
We have constructed an immunotoxin(Ng76-TCS),which was composed of a monoclonalantibody directed against human melanoma and trichosanthin(TCS)——a single chain ribosomeinactivating protein.The cultured human melanoma cells(M21)were inhibited effectively byNg 76-TCS.The cytotoxicity of Ng76-TCS to M21 cells was 2,000-fold higher than that of free TCS and Ng76 mixture.A conjugate,which was prepared with normal mice immunoglobulinand TCS(NIgG-TCS),was 160-fold less cytotoxic to M21 cells.Meanwhile Ng76-TCS was125-fold less cytotoxic to nontarget cells Hela.These results showed that the immunotoxinNg76-TCS was a potent and specific anti-human melanoma agent.
5. Prognostic analysis of colon and rectal neuroendocrine neoplasm in different stages
Yuting HUANG ; Ru JIA ; Qian XU ; Shoujian JI ; Heteng CUI ; Jianming XU
Chinese Journal of Oncology 2019;41(2):146-151
Objective:
To explore the survival difference of patients with colon and rectal neuroendocrine neoplasm (NEN) at different stages.
Methods:
We identified 8 679 patients with colorectal NEN diagnosed between 1988 and 2014 from the Surveillance, Epidemiology, and End Results (SEER) registry, including 5 437 rectal NEN and 3 242 colon NEN ( 1 681 cecum NEN ). Survival curve was drawn by Kaplan-Meier method. Prognostic factors were analyzed by univariate analysis and multivariate Cox regression model.
Results:
The ratio of male patients with colon and rectal NEN was similar to female (
6.Leptin gene C2549A polymorphism in minority Hui and Uygur children with obesity.
Ji-Hong ZHANG ; Wen-Juan ZENG ; Pei-Ru XU ; Wei-Ping ZHANG
Chinese Journal of Contemporary Pediatrics 2014;16(1):20-24
OBJECTIVETo investigate the relationship of leptin gene polymorphism with obesity in ethnic minority Hui and Uygur children in China.
METHODSSixty-eight ethnic minority (35 Hui and 33 Uygur) children with obesity and 69 age-matched minority (36 Hui and 33 Uygur) children without obesity were recruited from six primary schools in the sub-urban areas of Urumqi. Venous blood was sampled from all subjects after fasting for 12 hours. Leptin gene C2549A polymorphism was determined by polymerase chain reaction (PCR) and restriction fragment length polymorphism methods. Blood concentrations of lipids, leptin and insulin were measured with biochemical methods and radioimmunoassys, respectively.
RESULTSIn the 137 children tested, the prevalence of AA, AC and CC genotype was 9.5%, 33.6% and 56.9%, respectively. A allele frequency was significantly different between the two ethnic (i.e. Hui and Uygur) groups (P<0.05). A allele frequency and AA+ AC genotype frequency were not significantly different between obese and non-obese children in both ethnic groups (P>0.05). Blood leptin levels were not significantly different between obese and non-obese children with an AA+AC or CC genotype in both ethnic groups (P>0.05).
CONCLUSIONSLeptin gene polymorphisms exist in Hui and Uygur children. The C2549A polymorphism is not significantly associated with the prevalence of obesity in both Hui and Uygur children.
Child ; China ; ethnology ; Female ; Genotype ; Humans ; Leptin ; blood ; genetics ; Lipids ; blood ; Male ; Obesity ; blood ; genetics ; Polymorphism, Genetic
7.P-JAK2 and P-STAT3 protein expression and cell apoptosis following focal cerebral ischemia-reperfusion injury in rats.
Hui-Fang XIE ; Ru-Xiang XU ; Ji-Peng WEI ; Xiao-Dan JIANG ; Zhen-Hua LIU
Journal of Southern Medical University 2007;27(2):208-218
OBJECTIVETo investigate the changes in phosphorylated JAK2 and STAT3 protein expression of and cell apoptosis following focal cerebral ischemia-reperfusion injury in rats.
METHODSA rat models of focal cerebral ischemia-reperfusion injury was established by middle cerebral artery occlusion using modified filament method. Immunohistochemistry and Western blot analysis were used to detect the expression of P-JAK2 and P-STAT3 proteins, and TUNEL assay was employed to examine the cell apoptosis.
RESULTSP-JAK2 and P-STAT3 protein expression increased significantly after cerebral ischemia-reperfusion injury in rats. The immunoreactivity was prominent in the peripheral of the ischemic region and reached the peak level at 24 h of reperfusion, followed by slight decrement. The apoptotic cells increased obviously after cerebral ischemia-reperfusion injury, also reaching the peak level at 24 h of reperfusion.
CONCLUSIONThe expression of phosphorylated JAK2 and STAT3 may be involved in the ischemic cellular events including apoptosis. JAK2/STAT3 signaling pathway plays a role in the pathophysiological process of cerebral ischemia/reperfusion cell injury and repair.
Animals ; Apoptosis ; Blotting, Western ; Immunohistochemistry ; In Situ Nick-End Labeling ; Infarction, Middle Cerebral Artery ; physiopathology ; Janus Kinase 2 ; metabolism ; Male ; Phosphorylation ; Rats ; Rats, Sprague-Dawley ; Reperfusion Injury ; genetics ; physiopathology ; STAT3 Transcription Factor ; metabolism
8.Experimental studies and clinical application of quickly prefabricated random skin flap.
Jun XU ; Guo-an ZHANG ; Hui-ru MI ; Hong SU ; Xing-yi HAN ; Hui CHEN ; Da-xin CAO
Chinese Journal of Plastic Surgery 2004;20(3):165-168
OBJECTIVETo enlarge the ratio of length to width of a prefabricated random skin flap in a short period, in order to meet a special clinical need.
METHODSA white rat and a white mini-pig was chosen for an animal model for the experiment. They were divided into the experimental group and the control. The prefabricated flap was formed as the planned design. In the experimental group, the flaps were treated with a desired pressure in certain intervals at the planned part of the flap. We estimated and inspected the digitized perfusion of flap microcirculation, and made overcong and wide random skin flap survive within 24 hours, and this technique was finally used for the clinical wound treatment.
RESULTSAs expected, the survival area of the flap in the experimental group was significantly larger than the control. This technique was successfully used to repair the defects of the special sites in plastic and reconstructive surgery.
CONCLUSIONThe method of quickly prefabricated random flap could enlarge the ratio of length to width of the flap and put the flap in shape in short time. With the digitized estimating and inspecting, this method could obviously improve the survival area of the random flap and could also be very useful for the clinical treatment.
Adult ; Animals ; Dermatologic Surgical Procedures ; Female ; Humans ; Male ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Skin ; metabolism ; pathology ; Skin Transplantation ; methods ; Surgical Flaps ; supply & distribution ; Time Factors ; Transplantation, Heterologous ; Treatment Outcome
9.Investigation of sleep patterns and sleep disorders in Uigur and Hui children in Xinjiang.
Tajiguli ABUZHARE ; Pei-Ru XU ; Amanguli TIEMUER ; Ji-Hong ZHANG
Chinese Journal of Contemporary Pediatrics 2011;13(7):565-568
OBJECTIVETo study the sleep quality and quantity and their influencing factors in Uigur and Hui children in Xinjiang Province.
METHODSFrom March to December of 2007, 912 Uigur and 1019 Hui school children (6 to 14 years) were randomly sampled from 6 cities of Xinjing Province. The questionnaire on children's sleep states and sleep environments was filled in by children's parents.
RESULTSThe mean sleep time of Uigur and Hui children was 10.1±1.4 hrs. The sleep time in Uigur children was significantly less than that in Hui children (9.7±1.2 hrs vs 10.4±1.5 hrs; P<0.05). The mean incidence of sleep disorders was 23.56%. The Uigur children had a lower incidence of sleep disorders compared with the Hui children (18.42% vs 28.16%; P<0.001). Ethnic group, sleep latency, use of a swing bed, family history of snore, watching TV before sleeping, hypertrophy of tonsils, eating before sleeping, feeding patterns and recurrent upper respiratory tract infection were the factors associated with the sleep quality and quantity.
CONCLUSIONSThe influencing factors of sleep quality and quantity are multifactorial in Uigur and Hui children in Xinjiang Province. Pediatrician, child health care doctors and parents should pay more attention to children's sleep. The incidence of sleep disorders is different between Uigur and Hui children, suggesting that more research for the prevention and treatment of sleep disorders should be performed in different ethic groups.
Adolescent ; Child ; China ; epidemiology ; ethnology ; Female ; Humans ; Logistic Models ; Male ; Risk Factors ; Sleep ; physiology ; Sleep Wake Disorders ; epidemiology ; ethnology ; etiology ; Time Factors
10.Clinical features of hepatitis B e antigen negative chronic severe hepatitis B patients.
Qing-feng SUN ; Wen-bing WANG ; Dao-zhen XU ; Yun-ru LI ; Ji-guang DING
Chinese Journal of Hepatology 2007;15(1):70-72
Adult
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Female
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Hepatitis B e Antigens
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blood
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Hepatitis B, Chronic
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blood
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diagnosis
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Humans
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Male
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Middle Aged
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Prognosis