Objective To analyze risk factor in children with nosocomial infection of pediatric department. Methods 3 250 children were collected as observation group in recent five yesrs.The infective characteristic were analyzed retrospectively in observation group.Results 73 hospital infective cases were found in the observation group.50 cases of pulmonary infection,10 cases of digestive tract infection,10 cases of urinary system infection,and 3 cases of oral infection.Age(χ2 =5.76,P <0.05 ),duration of hospitalization(χ2 =6.05,P <0.05 ),invasive operation(χ2 =7.75,P <0.05)and preventive antibiotics use(χ2 =7.86,P <0.05)were correlated with nosocomial infection.Staphylococcus aureus and Staphylococcus haemolyticus of G + strain was most,staphlococcus epidemidis and klebsiella of G -strain was most.Conclusion The risk factors should be paied attention in paediatric cases,as well as strengthen the strict aseptic operation and apply with bacterial culture of secretion,which is of significance to guide clinical medication.

Objective To evaluate the effect of color Doppler ultrasound monitoring in artificial arteria-venous fistula in patients with uraemia.Methods 25 cases were examined with upper limbs arteriovenous fistulas with uremia and analysed by CDFI and 2D.The fistula and blood vessel was observe and hemodynamics changes were examined.Results Among 25 patients,18 cases showed good filling state of the vessel,3 cases with anastomoses stenosis,2cases with thrombus and 2cases with venous dilatations.Conclusion CDFI is an effective supplementary detective methods in treating hemodialysis patients with uremia.

Objective To explore the influence of ICAM-1 and LFA-1 on the breast cancer metastasis and provide scientific base for studying the molecular mechanism of breast cancer metastasis by observing the expression of ICAM-1(intercellular adhension molecule1) and LFA-1(Lymphocyte function related antigen 1) in different time different metastasis stage of breast cancer. Methods We observed the expression of ICAM-1 and LFA-1 in breast cancer tissue as well as the expression of ICAM-1、LFA-1 and VEGFR-3 in Lymphatic vessel endothelial cell of nearby the breast cancer tissues from the patients of the operation in the defferent stages with the methods of HE staining and immunohistochemistry staining. Results The positive rate of ICAM-1 and LFA-1 expression in cancer cell and cancer nest of breast cancer was increased gradually along with the increase of clinical stages and the occurrence of lymph node metastasis. And the positive rate of ICAM-1、LFA-1 and VEGFR-3 expression in lymphatic vessel endothelial cell of nearby the breast cancer tissues was increased gradually too along with the increase of clinical stages and the occurrence of lymph node metastasis,which has the positive correlation,and it has the positive correlation that the ICAM-1 and LFA-1 expressed in Lymphatic vessel endothelial cell of nearby the breast cancer tissues(P0.05).Conclusion The ICAM-1 and LFA-1 were related with breast cancer lymph metastasis,and the ICAM-1 and LFA-1 promoted breast cancer lymph metastasis through synergism each other.The increase or decrease of ICAM-1 and LFA-1 expression in the breast cancer tissue and lymphatic vessel endothelial cell of nearby the breast cancer tissues could as the data target to display certain instruction function for prevention and treatment of breast cancer lymph metastasis.The Lymphatic vessel quantity of nearby the breast cancer tissues was increased along with tumor progress, which may be related with breast cancer lymph metastasis.VEGFR-3 has the high specificity in the lymphatic vessel endothelial cell expression,and may act as the lymphatic vessel as the lymphatic vessel endothelial cell specific marker.

Idebenone is a synthetic analogue of coenzyme Q10. As a potent antioxidant,idebenone operates under low oxygen tension situations, protects cell membranes and mitochondria from oxidative damage through inhibiting lipid peroxidation, thereby protects against cerebral ischemia and nervous system damage. Idebenone also interacts with the mitochondrial electron transport chain and maintains the formation of ATP under ischemic conditions. Because idebenone has a good tolerability and safety, it is expected as a neuroprotective agent for the treatment of acute ischernic stroke. Studies in recent years have showed that idebenone has a certain therapeutic effect in a variety of nervous system diseases involving mitochondrial dysfunction and oxidative stress damage, such as mitochodrial encephalomyopathy, lactic acidosis, stroke-like episodes syndrome, Friedreich's ataxia, Alzheimer's diseases, Leber's hereditary optic neuropathy, and Duchenne muscular dystrophy. At present, some clinical trials in the mitochondria-related diseases and neuromuscular diseases are underway, and their results are expected to further expand the indications of idebenone.

Objective To construct safety culture evaluation system for intravenous treatment. Method On the basis of literature review and in-depth interview of two rounds using Delphi method , consensus was achieved to build an evaluation system for safety culture of intravenous treatment . Results The recovery rate from expert consultation questionnaires of two rounds was 90 . 00% and 94 . 40%, respectively . The expert′s authority coefficient was greater than 0 . 80 . The expert′s coordination coefficient was 0 . 308 , with significant difference statistically ( P < 0 . 05 ) . After two rounds of expert consultation , an evaluation index system for safety culture of intravenous treatment was formed , including five 5 primary indexes and 25 secondary indicators . The experts had consensus on the indexes . Conclusion The evaluation system can help us to understand the status quo of intravenous treatment′s safety culture so that we can make targeted improvement . It is of importance for guaranteeing the safety of patients with intravenous therapy .

Objective To evaluate the effectiveness and method of intraarterial embolization in treatment of hepatic cavernous hemangioma. Methods 17 patients,8males and 9 females, were treated by super selective hepatic artery embolization with iodized oil, gelfoam pieces, and embolization coils. Results All patients had been embolized successfully. The rate of success was 100%. 11 cases were followed up by CT/USG for 1～12 months. The size of tumor was decreased more than 50% in 8 cases, more than 30% in 2 cases, and the effect was uncertain in 1 case. Symptoms of 11 patients were disappeared and relief was obvious in 5 cases.Total effective rate was 94% with no significant complications. Conclusions HAE is a safe and effective method. It may be considered as the first method of choice in treatment of hepatic cavernous hemangioma.

Purpose:To study the pathological and clinical characteristics of different subtypes of cyst-associated renal cell carcinoma and summarize the experience in diagnosis and therapy. Methods:39 cases with cyst-associated renal cell carcinoma underwent urological operations in our hospital from 1991 to 2000. Their medical data were analyzed and then divided into different subtype according to pathological characteristics. Results:The data included 2 patients with renal cell carcinoma on the background of polycystic kidney, 20 cases with renal cell carcinoma originating in a cyst and 17 cases with cystic renal cell carcinoma.3 patients underwent partial nephrectomy and 36 cases unilateral radical nephrectomy.16 patients were Robson stage Ⅰ and 23 were RobsonⅡstage.The pathological diagnosis was 38 cases of clear cell carcinoma and one of chromophobe cell carcinoma. The survival rates at 1,3,5 years are 100%、100%、94.8%(37/39) respectively after follow-up. Conclusions:The cases with renal cyst need periodical health check-up.Most of cyst-associated renal cell carcinomas belong to low stages. Contrast-enhanced computed tomography and selective digital subtraction angiography of renal artery are the main methods for preoperative clinical diagnosis. The long-term effect of operations of cyst-associated renal cell carcinoma is better.

Objective:To study the efficacy and immunoregulation of pidotimod combined with routine treatment in the children with recurrent respiratory tract infections. Methods:Totally 114 cases of children with recurrent respiratory tract infections were divid-ed into the control group and the treatment group according to the sequence of hospitalization. The 58 patients in the control group re-ceived the conventional treatment, while the 56 patients in the treatment group were given pidotimod additionally. After two-month treatment, all the children were given 1 -6-month follow-up, and the symptom disappearance time, clinical efficacy, recurrence, physiological indices and change of immunological function in the two groups were observed and compared. Results:The disappearance time of symptom and lung physical signs in the observation group was much shorter than that in the control group (P<0. 05);the total effective rate of the observation group was 92. 86%, which was significantly higher than that of the control group (67. 24%, P <0. 001). The number of upper and lower respiratory tract infections in the observation group was significantly lower than that in the con-trol group (P<0. 05). After the treatment, the immune parameters in the observation group were significantly improved (P<0. 01), which were significantly higher than those in the control group (P<0. 05 or P<0. 01). The immune parameters before and after the treatment in the control group showed no statistically significant difference (P>0. 05). Conclusion:Pidotimod combined with routine treatment in the children with recurrent respiratory tract infections exhibits promising efficacy, which can improve immunity effectively.

Objective To investigate the safety and therapeutic effect of intra-carotid infusion with cold sa line in rats with acute focal cerebral ischemia-reperfusion injury.Methods 60 rats were randomly divided into six groups:sham operated group,normal infusion group,stroke group,local hypothermic group,local normothermic group,and systemic infusion group.Brain infarct volume and cerebral water content were analyzed 48 h after ischemia.Neurological deficits were assessed using the mNSS 24 h and 48 h after infarction.Results In the local hypothermic group,brain temperature was reduced to 33 to 34 ℃ within 5 to 10 minutes,and this significantly low temperature maintained to nearly 60 minutes after infusion continued.Physiological variables were not significantly different among each time point (P ＞ 0.05).No significant morphological abnormality was found in brain sections stained with TTC and HE.Animals receiving local cold infusion significantly decreased infarct volume and brain water content compared to stroke group (P ＜ 0.05).Both 24 h and 48 h mNSS in local hypothermic group was significantly lower than those in other groups (P ＜ 0.05).Conclusions Intra-carotid infusion with cold saline can quickly and effectively reduce brain temperature and is a relatively safe cooling method.Local hypothermia significantly reduced brain infarct volume,decreased brain water content and improved neurological functional outcomes after brain ischemia.