Catecholaminergic polymorphic ventricular tachycardia is a rare hereditary ion channel disease, which mainly affects children and young adults.Clinically, the pathogenic genes of CPVT in adolescents are mainly RYR2, CASQ2, TRDN, CALM1 and TRD.Currently, beta blockers remain the first-line treatment for CPVT, and moderate exercise training is considered as an effective potential synergism for CPVT patients.Implantable cardioverter defibrillators are commonly used in the treatment of CPVT, but inappropriate shocks and device-related complications need to be carefully considered before implantation.Flecainide has been shown to be effective therapeutic agent, especially for the treatment of resistant CPVT cases with beta blockers.Left cardiac sympathetic denervation has also shown to be highly effective in preventing CPVT symtoms and cardiac events, and a combination of beta blockers can precede implantable cardioverter defibrillator implantation.

The scar tissue, resulting from aberrant process of wound healing , can bring about cosmetic deformeties and func-tional limitation, even the displacement of tissues and organs .The widely use of autologous fat graft in regenerative medicine offers a relatively new option for scar treatment .Lipofilling has improved symptoms and appearance of scars in several studies .The application , therapeutic effect and mechanism research of autologous fat graft for scar treatment are summarized in this paper .

The immunosuppressive effect of supernatant of the cultured tumor cells, including breast adenocarcinoma ( MCF-7, MDA-453 ), gastrocarcinoma ( MKN-45 ), cervical cancer ( Hela ) and ovarian cancer ( 3AO ), was studied by MTT assay. The results showed that the supernatant of tumor cell culture suppressed the proliferation of lymphocytes activated with PHA. The supernatant of cultured tumor cells, except that of cultured MDA-453 cells, also suppressed the cytotoxic activity of LAK cells. It is suggested that there are immunosuppressive factors in the supernatant of tumor cell culture. But the immunosuppressive effect of supernatant derived from the cultured tumor cells treated with sodium phenylacetate that is a noncytotoxic differentiation inducer on the proliferation of lymphocytes activated with PHA and the cytotoxic activity of LAK cells , was decreased significantly. It indicates that sodium phenylacetate could decrease the immunosuppressive factors derived from tumor cells.

Objective: To develop a method to determine the content of a berberine hydrochloride in Xiao A Lotion. Methods: HPLC was used to determine the content of berberine hydrochloride in Xiao A Lotion. The separation was performed on Inertsil ODS 3 column with 0.05mol/L NaH 2PO 4 (pH3.0) acetonitrile (70∶30) as a mobile phase and the wavelength of UV detector was 270nm. Results: The Linearity of this method was well with the average recovery of 101.4%. Conclusion: The method is simple, reliable, and sensitive. It also shows good separating degree. It can be used in quality control of Xiao A Lotion.

Object To isolate and identify the chemical constituents of Saussurea japonica (Thunb.) DC. and to obtain some rules of chemical shift effects on hydroxylation and O-glycosylation. Methods The constituents were isolated by silica gel column chromatography and HPLC (RP-18), and their structures were elucidated through spectroscopic analysis (NMR and FABMS). The rules were obtained by comparison with those of known analogous lignans. Results Three lignan glycosides were isolated and identifiedcas (+)-1-hydroxypinoresinol-4″-?-D-glucopyranoside (Ⅵ), (+)-lariciresinol-4-?-D-glucopyranoside (Ⅷ), and (+)-1-lariciresinol-4′-?-D-glucopyranoside (Ⅸ), as well as the rules for effects of the hydroxy group at C-1 (upshift about ??-4.2) and O-glucosylation (downshift about ??+3.0) on 13CNMR chemical shifts of (+)-pinoresinol and (+)-lariciresinol. Conclusion All these compounds were obtained from the plants of Saussurea DC. for the first time and the rules should be as some important evidences to assign the position of hydroxylation and glycosylation.

[Qbjective]To observe the effects of Zidian prescription extracted by two kinds of process on ITP mice.[Methods] Using APS to inject into mice to induce the ITP model.Mice were divided into 6 groups: C,M,DC,X,S and G groups.The number of platelet on 7th day and 14th day were measured in mice.Meanwhile the weight on 7th day and 14th day and the degree of subcutaneous bleeding were recorded.[Results]There is no significant difference in weight and the number of platelet in all groups before the experiment.Compared with the C group,the weight is in down trend in M group on 7th day,the weight and the number of platelet were declined in M group on 14th day,the petechia was more clearly in M group.Compared with the M group,the weight is up trend in DC,X,S and G groups on 7th day,the weight and the number of platelet are enhanced in DC,X,S and G groups on 14th day,the petechia was decreased in DC,S and G groups significantly.Compared with the DC group,the number of platelet is decreased in X,S and G groups.Compared with the X group,the number of platelet is increased in S and G groups.[Conclusion] Zidian prescription extracted by two kinds of extraction process has an remedial effect on ITP mouse.There is no significant difference between the two kinds of extraction process.

Objective To analysis the effect of paclitaxel on CEA, CA19-9 and CA72-4 levels in patients with advanced gastric cancer.Methods46 patients who were diagnosed with advanced gastric cancer in Pinghu hospital of traditional Chinese medicine were collected.All patients were randomly divided into experimental group and control group,23 cases in each group.The control group was treated with cis-platinum complexes and capecitabine, and the experimental group was treated with paclitaxel and capecitabine, after the treatment, the serum levels of CEA, CA19-9, CA72-4, vascular endothelial growth factor, endostatin and clinical curative effect were detected in all patients.ResultsAfter treatment, compared with control group,①the serum level of CEA was lower in the experimental group (P<0.05);②the serum level of CA19-9 and CA72-4 were lower in the experimental group (P<0.05);③the serum level of VEGF was lower in the experimental group (P<0.05);④the serum level of Endostatin was higher in the experimental group (P<0.05);⑤The clinical efficiency of the experimental group was higher(P<0.05).ConclusionsThe paclitaxel can significantly reduce the serumCEA, CA19-9, CA72-4 and VEGF levels, elevated serum Endostatin level in patients with advanced gastric cancer, improve the treatment effect, and have a guiding significance for clinica.

Object To study the effects of the different Qinglongyi extracts (QE) on cell membrane fluidity of S 180 and H 22 tumor-bearing mice. Methods The tumor models of S 180 and H 22 were used. The oxter of the normal mice were injected S 180 tumor cell liquid and the abdomen of those were injected H 22 tumor cell liquid. Tumor-bearing mice were given QE by ip cold alcohol extract (191.37, 95.68, 47.84 mg/kg), hot alcohol extract (121.17, 60.58, 30.29 mg/kg), and cytoxan (20 mg/kg as positive control), normal saline (as negative control) for 7 d then the mice were killed, and the tumor cell membrane liquids were made according to the methods. The membrane fluidity was studied with fluorescence polarization (P) and microviscosity (?) with 1,6-dipheyl-1,3,5-hexatriene (DPH) as a probe. Results The QE by both cold and hot alcohol can make the membrane fluidity of S 180 tumor cell improve, the membrane fluidity of H 22 tumor cell decline. Compared with the normal saline control group, QE by both cold and hot alcohol can make the membrane fluidity of RBC (red blood cell) decline (P

Objective: To explore the clinical value of N-terminal Pro-brain natriuretic peptide (NT-ProBNP) and cardial troponin I (cTnI) for evaluating the patients with acute pulmonary embolism (APE) combining right ventricular dysfunction (RVD). Methods: A total 92 APE patients treated in our hospital were studied. The blood levels of NT-ProBNP and cTnI were measured on next morning of administration and echocardiography was performed within 24 hours of APE diagnosis. According to echocardiographic results, the patients were divided into 2 groups: APE with RVD group,n=47 and APE without RVD group,n=45. Blood levels of NT-ProBNP and cTnI were compared between 2 groups. Results: Compared with APE without RVD group, APE with RVD group presented increased levels of NT-ProBNP, cTnI and right ventricular end-diastolic diameter (RVEDD), allP<0.01. ROC curve analysis found that both AUC of NT-ProBNP and cTnI > 90%, the optimum operating point for NT-ProBNP and cTnI were 554 pg/ml and 0.09 ng/ml respectively. Logistic regression analysis showed that NT-ProBNP (r=1.227,P=0.005), cTnI (r=0.862,P=0.016), right ventricular dysfunction (r=0.936,P=0.008) and heart rate (r=0.809,P=0.023) were related to poor prognosis for in-hospital patients. Conclusion: Blood levels of NT-ProBNP and cTnI may help diagnosing the patients with acute pulmonary embolism with right ventricular dysfunction, it may also predict the short term prognosis in clinical practice.

Objective To evaluate the effect of OTD(observation-teaching-discussion) method in teaching of kinesiology of rehabilitation medicine.Methods 110 students majoring in rehabilitation medicine were divided into two groups,routine teaching group(56 students) and OTD teaching group(54 students),and the teaching effect was evaluated by exam score and questionnaire.Results There were obvious difference in the exam score and questionnaire between two groups(P<0.01).Conclusion In the teaching of medical rehabilitation,OTD method is more advantageous than routine method in bring up students' study interest,the initiative spirit,and the ability of self-study and analyzing.