Objective To investigate the biochemical differences between the cartilage of the residual ear of microtia patient and normal person. Methods Seven cartilage specimens from 7 about 10-years-old microtia patient's residual ears and 7 normal ears from cadaver of the same age were measured by electrophoretic mobility shift assay and spectrophotometer for collagen glycoaminoglycan (GAG), chondroitin sulfate (Chs), hyaluronan (HA) and keratin sulfate (KS) .Results Biochemical analysis showed that no significant difference were found in the collagen contents of the two groups. The microtia cartilage had more GAG (49.00?25.60) ?g/mg than that of the normal (28.25?4.80) ?g/mg. The composition of GAG were different between two groups: microtia group (HA 38.96+4.97 %, Chs 29.02 %?4.12 %, KS 32.16 %?7.41) % and normal group (HA 32.94 %?3.24 %, Chs 33.10 %?2.61%, KS 33.96 %?1.66 %). There were differences in HA and Chs relative contents, but not in KS between the two groups. Conclusions There is no difference in the collagen content between normal and microtia ear cartilage. But the latter has more GAG than the former. As for the constitution of GAG, microtia cartilage has a higher percentage of HA, lower percentage of Chs, and no significant difference in KS.

Objective To select optimum water extraction process of SuXingErChen Syrup by orthogonal test and multivariate nonlinear regression analysis.Methods Naringin was extracted by water, Used the naringin content and extraction rate of extractum as indexes.Based on the orthogonal test and multivariate nonlinear regression analysis to make sure the best way for extracting naringin from SuXingErChen Syrup.Results The optimal conditions of water extraction process was that the herbs were added seven times the amount of water without infiltration,extracted two times with 80 min each time,which was selected by multivariate nonlinear regression analysis.Conclusion The process of water extraction for the preferred Su XingErChen Syrup is exact, high effective and suitable for production needs.

Computer technology is one of the greatest achievements of the 20th Century. Its appearance not only brings a breakthrough development in various fields, but also brings promotion to the development of traditional Chinese medicine (TCM). TCM is a traditional medicine, and the modernization of TCM is urgent. Whether in the medical management or scientific research, the application of computer technology has penetrated into all levels. The following article described the contribution made by computer technology, pointed out possible problems and provided relevant thinking based on the application of TCM related systems and data mining technology.

Animals ; Humans ; MicroRNAs ; genetics ; Neoplasms ; genetics ; pathology ; RNA Interference ; RNA, Neoplasm ; genetics ; RNA, Small Interfering ; genetics

Since the definition of sepsis has been first proposed in 1991, the main content of the definition has been developed by infection + systemic inflammatory response syndrome as infection + organ dysfunction,and organ dysfunction is indicated by an increase in sequential organ failure assessment(SOFA) score.These are components of Sepsis-3 which was published in 2016.In Sepsis-3,the SOFA score in the identification and diagnosis of sepsis plays an important role,but Sepsis-3 was mainly used in adults,how to adjust it to children,some questions and details were proposed in this article.

Purpose:To identify clinical predictors of stage ⅢA-N2 in patients with non-small cell lung cancer. Methods:From March 1995 to February 1998, 118 patients with pathological stage ⅢA-N2 non-small cell lung cancer who underwent a resection by Shanghai Chest Hospital were analysed. Prognostic factors were estimated from the date of operation using the Kaplan-Meier and log-rank analysis. The Cox regression model evaluated the influence of factors on the survival. Results:The overall of 3-year and 5-year survivals of the 118 patients were 40.5% and 18.4%. Margin, the number of N2 lymph nodes and stations, the cycles of adjuvant chemotherapy were associated with survival. In a multivariate analysis, the number of N2 lymph nodes and the cycles of adjuvant chemotherapy significantly influenced survival. Conclusions:The number of N2 lymph nodes and the cycles of adjuvant chemotherapy were important prognostic factors of stage ⅢA-N2 non-small cell lung cancer.

Objective To investigate the possibility of using poly-hydroxybutyate-hydroxyhexate p(3HB-co-3HH)), a modified extracellular matrix, the third generation of PHA family, as a scaffold for seeding of chondrocytes from the residual ear of microtia patients to generate tissue engineered cartilage in athymus mice. Methods The residual ear cartilage from eight 7-years-old microtia patients was enzymatically dissolved by collagenase, and chondrocytes were harvested and seeded into foams of PHB-PHH. After incubation for 1 week in vitro, chondrocyte-polymer constructs were implanted subcutaneously into 8 athymus mice. A control groups was established by subcutaneous implantation of PHB-PHH foams alone. One athymus mice were killed at 4 weeks postoperatively, and 6 were sacrificed at 8 weeks. The specimens were dissected and examined macroscopically and histologically. Results Specimens harvested from chondrocyte-polymer constructs subjected to gross morphologic and histology analysis demonstrated new cartilage formation, and those from control groups showed no cartilage formation. The one of 4 weeks still had some remains of the scaffold with nodules of neocartilage. After 8 weeks, all the 6 mice had neocartilage formed almost the same as natural. The PHB-PHH scaffold were totally absorbed. Conclusion These findings demonstrate that the foam of PHB-PHH is not only a good "matrix" for cartilage tissue engineering, but also optimal scaffold for the seeding of chondrocytes from the residual ear to generate new cartilage that would be useful in plastic and reconstructive surgery. Chondrocytes from the residual ear of microtia patients are good candidates for generation of tissue engineered cartilage.

Objective To study the growth and oxidative damage of rats exposed to mercury mining area rice. Methods The rats were exposed to the rice harvested in mercury mining area or in other area for 90 days, and then determined the change of their weight, the activity of SOD and GSH-Px, the content of MDA and GSH in brain, liver and kidney tissue. Results After 90 days of exposure, compared with the control,significantly higher weight,higher level of MDA,lower activities of GSH-Px,SOD and lower level of GSH were found in brain, liver and kidney tissue of the rat of exposure group. Conclusion The results of the present paper show that the rice contaminated by mercury in this mercury mining area may induce lipid peroxidation in the rat's in brain, liver and kidney tissue.

OBJECTIVE:To analyze bacteria distribution and drug resistance of pediatric severe sepsis in our hospital,and to provide reference for clinical rational use of antimicrobial agents. METHODS:57 pediatric severe sepsis patients were collected from pediatric intensive care unit of our hospital during Jan. 2014 to May 2015. The results of pathogen culture and drug sensitivity tests were analyzed retrospectively. RESULTS:Of 57 children,pathogen were detected in 18 cases(31.58%). A total of 91 pathogen were detected,of which there were 24 strains of Gram-positive(G+)bacteria(26.37%)mainly including Staphylococcus and Entero-coccus,60 strains of Gram-negative (G-) bacteria (65.93%) mainly including Klebsiella pneumoniae and Acinetobacter calco-acetcus-A. baumannii complex and 7 strains of fungus (7.69%) as Candida. 4 strains of methicillin-resistant Staphylococcus,22 strains of carbapenems-resistant K. pneumoniae,21 strains of multi-drug resistant K. pneumoniae and 7 strains of multi-drug resistant A. calcoacetcus-A. baumannii complex were all detected. Methicillin-resistant Staphylococcus,Staphylococcus aureus and Streptococ-cus pneumoniae were sensitive to vancomycin and linezolid,with resistant rate of 0. K. pneumoniae was completely resistant to ampi-cillin sodium and sulbactam sodium,piperacillin sodium and tazobactam sodium,imipenem and cephalosporin,with resistant rate of 100%. Resistant rate of A. calcoacetcus-A. baumannii complex to major common antimicrobial agents was higher than 50%. Esche-richia coli was resistant to cefotaxime,and resistant rates of other antimicrobial agents were lower than 40%. CONCLUSIONS:Main pathogen of pediatric severe sepsis is G- bacteria in our hospital,and carbapenems-resistant K. pneumoniae is detected,to which should be pay attention. The multiple drug-resistant treatment should be adopted for pediatric severe sepsis caused by multiple drug-re-sistant bacteria. Antimicrobial agents should be selected rationally according to pathogen type and the results of drug sensitivity test.

Many researches have focused on the wnt-frizzled cascade in the recent years, while much work has been done in neoplastic diseases and embryology, the role of the wnt-frizzled signal transduction pathway in cardiovascular diseases has only recently begun to be explored. It plays a very important role in many physiological and pathophysiological processes, such as its transduction pathway, the healing after myocardial infarction, the proliferation, differentiation and orientation of cardiomyocytes, angiogenesis/neovascularization, cardiac hypertrophy and heart failure, the deposition of the extracellular matrix and so on. This article is aimed at its relation with myocardial infarction and the role of this pathway in cardiovascular diseases.[