In tissue engineering, alginate has been an attractive material due to its biocompatibility and ability to form hydrogels, unless its uncontrollable degradation could be an undesirable feature. Here, we developed a simple and easy method to tune the degradation profile of the fibrous alginate scaffolds by the microfluidic wet spinning techniques, according with the use of isopropyl alcohol for dense packing of alginate chains in the microfiber production and the increase of crosslinking with Ca²⁺ ion. The degradation profiling was analyzed by mass losses, swelling ratios, and also observation of the morphologic changes. The results demonstrated that high packing density might be provided by self-aggregation of polymer chains through high dipole interactions between sheath and core fluids and that the increase of crosslinking rates could make degradation of alginate scaffold controllable. We suggest that the tunable degradation of the alginate fibrous scaffolds may expand its utilities for biomedical applications such as drug delivery, in vitro cell culture, wound healing, tissue engineering and regenerative medicine.
2-Propanol ; Cell Culture Techniques ; Hydrogel ; Hydrogels ; In Vitro Techniques ; Methods ; Microfluidics* ; Polymers ; Regenerative Medicine ; Tissue Engineering ; Wound Healing

Over Past years people have known rich medical information and knowledge through mass-media. It is needed to develop medical database, which makes people get proper medical information, So we made an effort to analysis health and medical consultation data. People easily access internet hospital of Gachon Medial School Gil Medical Center, coorperated with MIDAS Dongailbo. 4020 cases of "Questions and Answers "were conducted to access from March 25th, 1997 to March 25th, 1998. Using Java Servlet, Sybase, and Apache Web Server, we classified data. Those data present over 4000 databases, such as text (3644 cases), image (212 cases), moving picture (21 cases), and medical dictionary (416 dases). People can search symptome, or disease, what they want to know. Our database as a medical service is enhance people know about medical knowledge and information, so that they can take care of their health and prevent illness in advance.
APACHE ; Dictionaries, Medical ; Humans ; Indonesia ; Internet* ; Multimedia*