Aim To investigate interaction of CJX1,an amlodipine derivative,with ATPase activity of human P-glycoprotein.Methods Plasma membranes were prepared from K562/DOX cells,the P-gp adenosine triphosphatase activity of K562/DOX cell membranes was estimated by measuring inorganic phosphate liberation.Results The basal P-gp ATPase activity was increased by CJX1 with half-maximal activity concentration (Km) of (8.6?1.4) ?mol?L-1,CJX1 might interact with P-gp with a higher affinity and exhibit more potent effect than Verapamil (Ver).Kinetic analysis indicated a non-competitive inhibition of Ver-stimulated P-gp ATPase activity by CJX1 and a competitive inhibition of CJX1-stimulated P-gp ATPase activity by tetrandrine (Tet),moreover,the effect of CsA on CJX1-stimulated and Ver-stimulated P-gp ATPase activity showed a non-competitive and a competitive inhibition respectively.Conclusion CJX1 and Tet can bind P-gp on overlapping sites,while CJX1 and Ver as well as CsA can bind P-gp on separated sites in K562/DOX cells.

Objective:To reveal the actual living conditions and the vulnerability of the life quality of orphans with acquired immune deficiency syndrome (AIDS) in China,and to provide basis for appropriate relative health promotion strategies.Methods:Using gender-age 1∶ 1 matching design,96 pairs of AIDS orphans and children from ordinary families in a county of Henan Province were investigated for their living conditions and subjective quality of life with the Inventory of Subjective Life Quality.Results:(1) Compared with the control group,AIDS orphans had lower life satisfaction (45.16% vs.62.37%,P=0.019),suffered more injustice and stigma (35.48% vs.12.90%,P<0.001),had more needs to work to earn money (55.91% vs.23.66%,P<0.001) and lower probability to receive medical treatments when they were sick (36.36% vs.78.85%,P<0.001).(2) The average total score of subjective life quality in orphan group was significantly lower than that in the control group[(145.9±16.0) vs.(154.9±16.1),P<0.001].Scores of four dimensions including family life,school life,living environment and self-awareness were all significantly lower in orphans than in non-orphans which resulted in the significantly lower awareness stratum scores.(3) The average total score of subjective life quality in male orphan group was significantly lower than that in the male control group[(143.2±16.7) vs.(155.8±17.1),P<0.001].Scores of four dimensions including family life,school life,living environment and self-awareness were all significantly lower in male orphans than in male non-orphans which resulted in the significantly lower awareness stratum scores.Conclusion:AIDS orphans have more problems in lives.Their life quality is significantly affected which mainly appears as the decline of self-awareness and environmental awareness level.Long-term impacts on emotion and mental health deserve special concern.Male orphans are more vulnerable than females.

Objective To investigate impact of abnormal slucose metabolism on major adverse cardiac event in patients after coronary artery stent implantation.Methods Two hundred and fifteen patients whose data were available were enrolled in this study,and the patients were divided into 3 groups,diabetes group(group A),abnormal glucose metabolism group(group B),normal glucose tolerance group(group C).The clinica,coronary artery lesion characteristics and major adverse cardiac event(MACE)rate during in hospital and follow up periods were compared.Resuits There was a higher occurrence of MACE in group A and group B than that in group C(P＜0.01).There was no significant difference between group A and group B.Conclusion The patients of coronary heart disease with abnormal slucose metabolism have higher MACE rate than abnormal slueese metabolism.

Objective To investigate the growth,development,nutritional status and mental health of AIDS affected orphans in China and provide scientific basis for policy formulation.Methods Adopting gender-age 1∶1 matching design,96 pairs of AIDS affected orphans and children from normal families were investigated in term of their physical and mental health status.Results Orphans were found thinner than non-orphans for their significantly lower BMI z scores and subscapular skinfold thickness.Compared with the control group,AIDS affected orphans also had significantly lower SES and BDI scores.Male orphans were more vulnerable than male non-orphans in physical and mental health and no significant differences were found between female groups.Conclusion AIDS affected orphans in China have significant physical and mental health vulnerabilities and urgently needs health care.More sustainable fostering strategies are needed to be explored and integrated health interventions with focus on mental health support will be crucial.And more attention should be given to male orphans.

Animals ; Dose-Response Relationship, Drug ; Humans ; Inhalation Exposure ; Mice ; Nanoparticles ; toxicity ; Rats ; Siphonaptera

Objective To study the anatomy,exposure and operation method in prevention of recurrent laryngeal nerve injury in thyroid surgery.Methods The clinical data of 520 patients with thyroid disease in treatment of operation were retrospectively analyzed,and exposure of recurrent laryngeal nerve in 268 cases,non exposure of recurrent laryngeal nerve in 252 cases; resection of the majority of thyroid in 232 cases,resection of ipsilateral thyroid lobe+ isthmus+on the side of most resection operation in 220 cases; resection of ipsilateral thyroid thyroidectomy+contralateral subtotal+ipsilateral lymph node dissection operation in 68 cases.Results Exposure of recurrent laryngeal nerve in 268 cases.The recurrent laryngeal nerve in the external laryngeal branch was single 214 cases,accounted for 79.9％ (214/268); two 42 cases,accounted for 15.7％ (42/268),more than 12 cases,accounted for 4.5％ (12/268).The laryngeal recurrent nerve during thyroid artery through the rear 143 cases,accounted for 53.4％(143/268) ; during thyroid artery through the front 92 cases,accounted for 34.3％ (92/268); walk line between thyroid artery branches 33 cases,accounted for 12.3％(33/268).The thyroid permanent damage rate of exposure of recurrent laryngeal nerve surgery was 1.1％(3/268),lower than that in non exposure of recurrent laryngeal nerve surgery 6.0％ (15/252) (P ＜ 0.05).The thyroid permanent damage rate between resection of the majority of thyroid and resection of ipsilateral thyroid lobe + isthmus + on the side of most resection operation had no significant difference (P ＞0.05),compared with the former two,resection of ipsilateral thyroid thyroidectomy + contralateral subtotal + ipsilateral lymph node dissection operation increased significantly (P ＜0.05).Exposure of.recurrent laryrngeal nerve in benign 173 cases,6 cases of thyroid transient damage,permanent damage in 1 case; 95 cases of malignant thyroid,6 cases of thyroid transient damage,permanent damage in 2 cases,the thyroid permanent damage rate in both had no significant difference (P ＞ 0.05).Conclusion Exposure of recurrent laryngeal nerve operation can significandy reduce the incidence of recurrent laryngeal nerve injury,for the partial thyroidectomy,can chose non exposure of recurrent laryngeal nerve operation method.

Objective:To study the behavior problems in children with Tourette syndrome and related family factors.Methods:132 children with Tourette syndrome were collected as our sample. Their parents completed CBCL (child behavior checklist) and FES (family environment scale).Results:The rate of behavior problems in this group was 26.5%, much higher than that of normal children (13.6%). The main factors having influence on this included expressiveness, independence and initiative, entertainment and moral religion. The Wald of the four factors in Logistic regression were 13.73, 10.35, 7.97 and 5.62(P

AIM To observe protective effects of doxepin on focal cerebral ischemia-reperfusion(I-R)injured rats. METHODS 120 rats were randomly divided into ischemia-reperfusion group,doxepin, nimodipine group and sham-operated group respectively, ischemia-reperfusion model was made by using the suture emboli method, the content of NO and MDA, activities of NOS and SOD in the cerebral tissues and the intracellular [Ca 2+ ] i in the cerebral cortex were determined respectively. RESULTS The content of NO, MDA and [Ca 2+ ] i and the activities of NOS decreased and the activities of SOD increased significantly in the doxepin and nimodipine group compared with ischemia-reperfusion group respectively(P

Objective To observe and compare the accurate dynamic changes of types Ⅰ, Ⅲ, Ⅴ, and Ⅵ collagens in the wound healing processes of the rabbit cornea undergoing laser epithelial keratomileusis (LASEK) or photorefractive keratectomy (PRK) and to investigate the possible mechanisms of corneal haze and myopic regression. Methods New Zealand White rabbits were divided into 8 groups: normal control group (n=6), 1, 7 d, and 1, 3, 4, 5, and 6 month groups (n=14). Every rabbit underwent LASEK in one eye while the other one with PRK. We used Western blot analysis to compare the wound healing process of dynamic changes of types Ⅰ, Ⅲ, Ⅴ, and Ⅵ collagens in the rabbit cornea at every time point. The results were analyzed with data analysis software. Results Western blot analysis showed that after LASEK, the cornea wound healing with types Ⅰand Ⅲ collagens were much faster than that of PRK, and the wound response was also much weaker. Whereas for types Ⅴ and Ⅵ collagens, their dynamic changes were similar between LASEK and PRK, and they both reached the peak value at 3 month after the surgery, but LASEK group returned to normal earlier than PRK group. The value of these two types of collagens after PRK was higher than that by LASEK. The changes of these four types of collagens may offer us at least partial explanation to the difference of formation of corneal haze and refractive regression. Conclusion There are significant differences between LASEK and PRK in types Ⅰ, Ⅲ, Ⅴ, and Ⅵ collagens or the time of reacting, reaching apex, and returning to normal. LASEK has slighter intensity of reaction. The results indicate that there is excessive aggradation of collagens after PRK, which may be the histological foundation of obvious haze and myopia regression.

Objective To investigate the effect of neoadjuvant chemotherapy on gastric cancer based on FOLFOX7 or XELOX,so as to achieve evidence of clinical practice.Methods From January 2005 to June 2010,a retrospective analysis was performed for those patients with gastric cancer and underwent surgery.The patients were divided into neoadjuvant chemotherapy group and conventional chemotherapy group according to the chemotherapy method.The clinical efficacy,Ro resection rate and survival rate were evaluated in two groups.Results A total of 185 cases were included in neoadjuvant chemotherapy group (FOLFOX7 or XELOX,113 cases) and conventional chemotherapy group (72 cases).There was no significant difference in general status between two groups (P ＞ 0.05).Complete remission was in 48 cases,partial remission was in 51 cases,stable was in 14 cases,progression of disease was in 0 case in neoadjuvant chemotherapy group,and the effective rate was 87.6％ (99/113).Complete remission was in 30 cases,partial remission was in 25 cases,stable was in 15 cases,progression of disease was in 2 cases in conventional chemotherapy group,and the effective rate was 76.4％(55/72).There was significant difference in the effective rate between two groups (P＜ 0.05).The R0 resection rate in neoadjuvant chemotherapy group was higher than that in conventional chemotherapy group [60.2％ (68/113) vs.38.9％ (28/72)],and there was significant difference between two groups (P＜ 0.01).There was no significant difference in survival rate between two groups (P ＞ 0.05).Conclusion Neoadjuvant chemotherapy can reduce the tumor stage and improve the R0 resection rate,but has no obvious advantage in improving the survival rate during follow-up period.