Objective:To observe the anesthetic effects of SumianxinⅡ combined with diazepam on the Beagle dogs in experimental periodontal surgery to Provide the reference. Methods:Healthy adult male Beagle dogs were anesthetized with SumianxinⅡat the doses of 0.05、0.1、0.15 ml/kg in the combination group and diazepam(0.1 ml) by hip intramuscular injection simultaniously. Added injection dosage was half of the first dosage. Healthy adult Beagle dogs in the contral group were only anesthetized with SumianxinⅡ at the doses of 0.05、0.1、0.15 ml/kg by hip intramuscular injection. The time of adding anesthetic was not sure in surgery. Anesthesia induction period,initial duration of anesthesia,and adverse reactions after anesthesia were compared between the two groups. Results: The combined treatment group induction dose respectively (11.00?1.41) min,(9.00?0.82) min,(12.75?0.50) min,For the first time maintaining anesthesia(29.75?6.62) min,(46.00?5.29) min,(45.25?6.02) min,Intraoperative additional one times longer cansustain anesthesia for(38.75?3.50) min,(45.25?4.03) min,(37.25?2.75) min. Pure Sumianxin Ⅱ new standards of sleep narcotic induction respectively respectively was (15.75?2.50) min,(14.25?2.99) min,(14.50?2.08) min,For the first time maintaining anesthesia was (20.75?5.19) min,(20.5?3.42) min,(22.25?5.19) min,the time of adding anesthetic was not sure in surgery. 2 Dogs died in SumianxinⅡ group. United drug group simple Sumianxin Ⅱ induction period in each group by t test,Sumianxin Ⅱat the doses of 0.1 ml/kg supplemented with 0.3 ml dose stability (P

The feasibility of screening for antibody to HIV by pooling 10 serum specimens was demonstrated in this experiment. The test was conducted using the reagent developed by Organon Teknika corporation (U. S. A) for testing for antibody to HIV by ELISA. The determination was made by changing only the ratio between the serum specimen volume and the serum specimen diluent volme, with their total volume being constant. When the serum specimen volume was increased from 5to 59?l as a result of pooling 10 serum specimens, while the serum specimen diluent volume was correspondingly decreased, the optical density of the pooled serum specimens was somewhat raised, but it was always below cutoff value, indicating that the specificity of test did not be subjected to any influence. In addition, when one serum specimen estabished as being positive intesting for antibody to HIV, and nine serum specimens from normal donors were pooled, the serum specimen containing the antibody to HIV had exactly been shown through multiple paired tests, indicating that the serumpooling method did not have any influence on the test sensitivity and could reach the level of original method. The serum-pooling method could be easy to spread because of its many advantages, such as saving the expenditure by 10 times, saving the manpower and material resource, and saving the working hours. (Original article on page 5)

Phototherapy is the most common and effective method to intervene and treat neonatal hyperbilirubinemia in clinic.The application of phototherapy varies from gestational age,birth weight,age and many other factors.Beside the traditional phototherapy instruments,more advanced high-intensive phototherapy instruments are applied to phototherapy.With the increasing application of phototherapy,many problems have showed up.This paper reviews based on the research of the indication,clinical application and adverse reactions on phototherapy.

Purpose　The aim is to investigate the effects of thymopeptide on LPS-induced release of nitric oxide (NO) from mouse peritoneal microphase in vitro.Methods　NO was detected by Griess reagent.Results　Thymopeptide 83.3～333.3 μg/ml reduced NO synthesis and secretion from mouse peritoneal microphase in vitro significantly.Conclusion　The effects of thymopeptide on LPS-induced release of NO from mouse peritoneal microphase may be one of mechanisms of its pharmacological effect.

OBJECTIVE:To investigate the effect of NGF on release of NO from peritoneal macrophages in mice METHODS:The content of NO in macrophages was detected with Griess agent RESULTS:NGF,in concentration of 0 73mg～5 83mg free amino acid per ml,could significantly inhibit release of NO from peritoneal macrophages in mice CONCLUSION:Inhibiting NO release may be one of the mechanism of NGF in protecting neurons

Child ; Glomerulonephritis ; classification ; diagnosis ; therapy ; Humans

Objective To investigate the efficacy of transurethral resection of bladder tumor(TURBT).Methods From August 2001 to January 2007,26 patients with bladder tumor were treated with TURBT.Cutting electrode was used to resect the tumor,including also the normal mucous membrane,away from the base of the tumor by 0.5-1.0 cm,and reaching shallow muscle layer of the bladder in depth.The wound was treated with electrocoagulation.Postoperatively,drugs such as mitomycin were used through intravesical instillation for chemotherapy.Results Operation time was 15-90 min(52.5?37.5 min).One case had serious postoperative bleeding of the bladder,1 case of bladder perforation.Follow-up was conducted in 22 cases for 0.5-2 years,(mean:1 year).Six cases had recurrence in which 4 were in situ,the other 2 were ectopia.One of the recurrent cases had chyluria,treated with ligation of renal lymphatic tissue.Conclusions TURBT has advantages such as simple surgical manipulation,and reliable efficacy etc,in treating bladder tumor.

Objective To investigate the expression of phosphoinositide 3-kinase (PI3K) and protein kinase B (Akt) and their role in pathogenesis in laryngeal squamous cell carcinoma. Methods Immunohistochemical S-P method was used to detect the expression of PI3K and p-Akt in 40 paraffin-embedded human laryngeal squamous cell carcinoma, 20 cases of polyp of vocal cords and 20 cases of laryngeal papilloma. All the samples were stained by HE method to confirm their pathologic characteristics. Positive rates of PI3K and p-Akt were evaluated by microscopy. Results PI3K and p-Akt showed low expression in the polyp tissues of vocal cords, while they were of significantly higher expression in laryngeal squamous cell carcinoma (P

Abortion, Habitual ; complications ; Antibodies, Antiphospholipid ; blood ; Antiphospholipid Syndrome ; complications ; pathology ; therapy ; Erythema ; complications ; Heart Valve Diseases ; complications ; Humans ; Kidney Diseases ; complications ; Skin Diseases ; complications ; Thrombosis ; complications

Fabry Disease ; diagnosis ; therapy ; Humans