PURPOSE: The inhibitory effect of TGFbeta1 on survivals of L1210 and anticancer drug- resistant L1210 sublines was investigated and the gene expression of TGFbeta1 in these cells was examined. MATERIALS AND METHODS: The survivals of L1210, adriamycin-resistant(L1210AdR), vincristine-resistant(L1210VcR) or cisplatin-resistant(L1210Cis) cells were measured by MTT assay after treatment of TGFbeta1. Northern analysis was performed for TGFbeta1 gene expression in L1210, L1210AdR, L1210VcR or L1210Cis. RESULTS: There was no different survival ratio between two groups, control and TGFbeta1(10 ng/ml) treated groups in L1210 cells. However, the survival ratio of L1210AdR was 59% in TGFbeta1 treated group for 96 hours. The survival ratio of L1210VcR was 61% for 96 hours in TGFbeta1 treated group. The survival ratio of L1210Cis was 40% for 96 hours in TGFbeta1 treated group. Expressions of TGFbeta1 gene in drug-resistant sublines were significantly decreased than that of L1210 cells. CONCLUSION: Growth of anticancer drug-resistant L1210 sublines were inhibited by TGFbeta1 but not in L1210 cells. So, it is suggested that TGFbeta1 gene expression may have a part in anticancer drug-resistance.
Control Groups ; Gene Expression

No abstract available.
Circumcision, Male* ; Female ; Male ; Sensation*

No abstract available.
Humans ; Hyperbilirubinemia, Hereditary* ; Siblings* ; Twins, Monozygotic*

No abstract available.
Chondrosarcoma* ; Sternum*

BACKGROUND: This study was conducted to compare the accuracy of computered tomography(CT) and sonography(US) in diagnosing acute appendicitis and to analyze the radiologic factors which influence the result. METHODS: We retrospectively studied the cases of 53 patients who were suspected of having appendicitis and who were examined by CT and US covered a three-year period. The final diagnosis was determined from the surgico-pathologic diagnosis and clinical follow-up for two months, as well as from the discharge diagnosis. RESULTS: Thirty-eight(38) patients had acute appendicitis; 15 patients did not. In the analysis, CT and US, respectively, revealed sensitivities of 89.5% and 73.7%, specificities of 93.3% and 60.0%, positive predictive values of 97.1% and 82.4%, negative predictive values of 77.8% and 47.4%. Among patients diagnosed with acute appendicitis, 10 patients were discordant in the US and the CT examinations; CT findings were correct in 8 and US findings in 2. Among non-appendicitis patients, 5 patients were discordant in the US and the CT examinations; CT was correct in 5 and US in 0. Nighteen(19) patients had complications such as an abscess or a perforation. Among these patients, the degree and the extent of the complication were accurately evaluated by CT in 15 patients and by US in 5 patients. CONCLUSION: CT is more accurate than US in diagnosing and excluding acute appendicitis. The use of CT will substantially decrease unnecessary laparotomies and post-operation complications.
Abscess ; Appendicitis* ; Diagnosis ; Follow-Up Studies ; Humans ; Laparotomy ; Retrospective Studies

Pulmonary thrombo-embolism in operating room is one of the important cause of morbidity and mortality in patients undergoing femur neck fracture surgery. However, the diagnosis of pulmonary thromboembolism may not be easy because sudden shock can have many different causes (e.g. myocardial infarction, hypovolemia, pneumothorax, non-cardiogenic pulmonary edema, pulmonary thrombo- embolism) and specialized diagnostic tools are not readily available in the operating room. Rapid and accurate diagnosis of pulmonary thromboembolism is very important in outcome of patients. We report a case in which pulmonary thromboembolism under spinal anesthesia occured just before the beginning of operation.
Anesthesia, Spinal* ; Diagnosis ; Femoral Neck Fractures ; Humans ; Hypovolemia ; Mortality ; Myocardial Infarction ; Operating Rooms ; Pneumothorax ; Pulmonary Edema ; Pulmonary Embolism* ; Shock

The authors have experience with a convulsive and comatoae patient who had intravenous regional anesthesia with 15ml of 2% lidocaine hydrochloride (total 300 mg). These complications were developed after sudden deflation of a pneumatic tourniquet, at the completion of debridement and pin fixation for traumatic open fracture on the right middle and ring fingers in a local clinic. This patient was treated at the I.C.U. of Busan National University Hospital without any other sequela. The authors re-emphasize the dangerous risks such as convulsions or even cardiac arrest when using a high concentration of lidocaine hydrochloride in intravenous regional anesthesia, as performed frequently.
Anesthesia, Conduction* ; Busan ; Coma* ; Debridement ; Fingers ; Fractures, Open ; Heart Arrest ; Humans ; Lidocaine* ; Seizures ; Tourniquets

The authors analyzed 508 cases of geriatric anesthesia over 60 years of age out of 12, 153 cases of total anesthesia performed from January, 1970 to December, 1977 at our department of B.N.U.H. and the results were obtained as follows. 1) Of the total 12 153 cases508 cases (4. 2%) were over 60 years of age, and these were subdivided into 303 male(59.7%) and 205 female (40. 3%). 2) Among the 508 cases of geriatric patients, age distributions were 397 cases(78.2%) in the age group of 60-69,93(18.3%)in 70-79 and 18(3.5%) in over 80. 3) The techniques of anesthesia were 311 cases(61.0%) of general anesthesia, 188(37.2%) of spinal and 9(1.8%) of the combination of these two. 4) The anesthetics were halothane 182 cases(35. 8%), tetracaine 197(38. 8%), diethyl ether 78(15. 4%), nitrous oxide 34(6. 7%) and methoxyflurane 17(3. 3%). 5) In the section of surgery, 190 cases(37.4%) were General Surgery, 116(22.8) Urology, 53(10. 4%) OrthOpedic Sulgery, alld 42(8. 3%) Ophthalmology in order of numbers. 6) For the duration of anesthesia, 334 cases(65. 8%) were in 1 to 3 hours, 70(13. 8%) within 4 hours, 56(11. 0%) within 1 hour and 48(9. 4%) over 4 hours. 7) Operation on head-neck were 122 cases(24. 0%) chest-upper abdomen 150(29. 5%), lower abdomen 104(20.5%), perineum 81(15. 9%) and extremities 51(10.1%). 8) Elective operations(327 cases, 64.4%) versus emergency(181 cases, 35.6%) was about 1.8: 1.
Abdomen ; Age Distribution ; Anesthesia* ; Anesthesia, General ; Anesthetics ; Clinical Study* ; Ether ; Extremities ; Female ; Halothane ; Humans ; Methoxyflurane ; Nitrous Oxide ; Ophthalmology ; Orthopedics ; Perineum ; Tetracaine ; Urology