No abstract available.
Pulmonary Atelectasis*

Dendritic cells (DCs) are key modulators that shape the immune system. In mucosal tissues, DCs act as surveillance systems to sense infection and also function as professional antigen-presenting cells that stimulate the differentiation of naive T and B cells. On the basis of their molecular expression, DCs can be divided into several subsets with unique functions. In this review, we focus on intestinal DC subsets and their function in bridging the innate signaling and adaptive immune systems to maintain the homeostasis of the intestinal immune environment. We also review the current strategies for manipulating mucosal DCs for the development of efficient mucosal vaccines to protect against infectious diseases.
Animals ; Dendritic Cells/*immunology/metabolism ; Humans ; Immunity, Mucosal ; Intestinal Mucosa/cytology/*immunology ; T-Lymphocytes, Helper-Inducer/immunology

BACKGROUND: Perfusion scintigraphy with dipyridamole have been reported to be useful for diagnosis of coronary artery disease and the assessment of the presence and extent of myocardium at ischemic risk, especially in patients who can not undergo dynamic exercise testing. Dipyridamole, pharmacologic coronary vasodilator, also induces fall in blood pressure and rise in heart rate. The purpose of this study was to answer the question if dipyridamole induced peripheral hemodynamic responses were related to chest pain, ST changes on EKG, scintigraphic defect or extent of coronary stenosis. METHODS: Dipyridamole 99mTc-MIBI myocardial scintigraphy and coronary angiography on 43 subjects who were suspected to have coronary artery disease. The peripheral hemodynamic response was graded as absent(group 0) if there was a < or =10mm fall in systolic blood pressure (SBP) and/or < or =10 beats/min rise in geart rate(HR) ; moderate(group 1) if there was >10 but < or =20mm fall in SBP and/or >10 but < or =20 beats/min rise in HR ; and marked (group 2) if there was >20mm fall in SBP and/or >20 beats/min rise in HR. RESULTS: The overall diagnostic sensitivity and specificity for coronary artery disease of dipyridamole perfusion scintigraphy were 68%, 83% while per vessel sensitivity and specificity for coronary artery disease were 66%, 97%. The numbers of induced chest pain and ischemic ST changes among hemodynamic subgroups, were 40%, 40% in group 0, 33%, 27% in group 1 and 50%, 40% in group 2 without significant difference in each hemodynamic subgroups. Either the numbers of diseased coronary arteries or the numbers of patients demonstrationg reversible scintigraphic defects were not statically different among each subgroups. CONCLUSION: Although the peripheral hemodynamic response dose not always correlate with its central coronary effect but dipyridamlole 99mTc-MIBI myocardial perfusion scintigraphy is an useful test for diagnosis of coronary artery disease.
Blood Pressure ; Chest Pain ; Coronary Angiography ; Coronary Artery Disease* ; Coronary Stenosis ; Coronary Vessels* ; Diagnosis ; Dipyridamole* ; Electrocardiography ; Exercise Test ; Heart Rate ; Hemodynamics* ; Humans ; Myocardial Perfusion Imaging ; Myocardium ; Perfusion Imaging ; Sensitivity and Specificity

Infection with invasive Shigella species results in intestinal inflammation in humans but no symptoms in adult mice. To investigate why adult mice are resistant to invasive shigellae, 6~8-week-old mice were infected orally with S. flexneri 5a. Shigellae successfully colonized the small and large intestines. Mild cell death was seen but no inflammation. The infected bacteria were cleared 24 hours later. Microarray analysis of infected intestinal tissue showed that several genes that are involved with the sphingosine-1-phosphate (S1P) signaling pathway, a lipid mediator which mediates immune responses, were altered significantly. Shigella infection of a human intestinal cell line modulated host S1P-related genes to reduce S1P levels. In addition, co-administration of S1P with shigellae could induce inflammatory responses in the gut. Here we propose that Shigella species have evasion mechanisms that dampen host inflammatory responses by lowering host S1P levels in the gut of adult mice.
Adult ; Animals ; Bacteria ; Cell Death ; Cell Line ; Colon ; Humans ; Inflammation* ; Intestines ; Mice* ; Microarray Analysis ; Shigella ; Shigella flexneri*

BACKGROUND: Selective transforaminal epidural block (STEB) has showen effectiveness as a diagnostic and therapeutic option for the management of patients with low back pain or sciatica. This study was carried out in order to determine the short-term effects and prognostic factors associated with STEB in patients with low back pain or sciatica. METHODS: Ninety-seven patients were selectedfor participation in this study. Their diagnosis were based werewason the clinical symptoms and MRI findings. We performed STEB under fluoroscopic guidance and injected 3 ml of radio opaque dye in order to confirm the technical success of the procedure. We then injected 20 mg of triamcinolone mixed into 3 ml of 0.5% mepivacaine. One month later, we classified the patient outcomes as excellent, good, moderate or poor, according to the degree of reduction in VAS score from baseline. The independent variables assessed included symptom duration, block level, number of blocks, primary diagnosis, prior caudal block, anterior epidural space filling of dye, medication history, demographic data, radiating pain, back surgery and spondylolisthesis. RESULTS: At a mean follow-up period of 1 month after STEB, excellent results were noted in the patients diagnosed with herniated lumbar disc (70%), non-specific spondylosis (54%), spinal stenosis (44%), and failed back syndrome (28%). The patients with epidural adhesion and combined spondylolisthesis were associated with poorer outcomes. Combined caudal block, symptom duration and the extent of epidural spread of the drug were not related to the effectiveness of the treatment. CONCLUSIONS: Selective transforaminal epidural block is effective in treating patients with radiculopathy, such as herniated lumbar disc, but it isrelatively ineffective in treating patients with structural deformities, such as failed back syndrome and spondylolisthesis.
Back Pain ; Congenital Abnormalities ; Diagnosis ; Epidural Space ; Follow-Up Studies ; Humans ; Low Back Pain* ; Magnetic Resonance Imaging ; Mepivacaine ; Radiculopathy ; Sciatica ; Spinal Stenosis ; Spondylolisthesis ; Spondylosis ; Triamcinolone

PURPOSE: Most malignant peritoneal or pleural effusions caused by advanced malignancy are unresponsive to systemic chemotherapy except for chemotherapy sensitive tumors, and they are equally ineffective to regional therapy or radiotherapy. Thus, for the purpose of palliating the symptoms related to malignant effusion and to reduce fluid reaccumulations, we evaluated the therapeutic feasibility and efficacy of intracavitary ' Ho-CHICO (chito- san complex) instillation for intractable malignant effusions. MATERIALS AND METHODS: Thirty one patients with cytologically or pathologically proven malignant effusions underwent intracavitary 166Ho-CHICO therapy from May 1996 to March 1998 at Ajou University Hospital. The subjective and objective responses were evaluated 4 weeks after the treatment, including the changes of symptoms, weight, abdominal girth, doses of diuretics, frequencies and amounts of repeat aspirations for fluid reaccumulations, and imaging studies of chest radiograph and ultrasounds. RESULTS: The response rates treated with Ho-CHICO were 50% in patients with peritoneal effusion and 46% in patients with pleural effusion (overall 49%). The response rates between 166Ho-CHICO doses of 50-80 mCi and 90-100 mCi were similar (50% vs 47%). Response rate of 70% was noted in patients with even distribution of radioisotope on the post-therapy scan, but, the response rate was lower in cases with focal (44%) and uneven (29%) distribution pattern. There was no difference in response by the effusion sites. All patients tolerated intracavitary 166Ho-CHICO instillation well, although the majority of patients experienced Grade I/II side effects such as pain, fever, weakness and dyspnea. But, no serious complications of Grade lII or IV degree were observed with 166Ho-CHICO therapy. CONCLUSION: Intracavitary 166Ho-CHICO instillation was clinically efficacious in controlling malignant effusions without a significant toxicity seen with conventional sclerotic therapy. The therapeutic modality appeared to offer similar benefits obtained with the conventional intracavitary therapy.
Ascitic Fluid ; Aspirations (Psychology) ; Chitosan* ; Diuretics ; Drug Therapy ; Dyspnea ; Fever ; Holmium* ; Humans ; Pleural Effusion* ; Radiography, Thoracic ; Radiotherapy ; Ultrasonography

BACKGROUND: Although cardiopulmonary resuscitation(CPR) is a very effective therapy in cardiac arrest, it is hard to prove the true effectiveness of CPR. Several studies about out-of-hospital and emergency department CPR exist, but only a few reports about in-hospital CPR are available. This study was designed to investigate in-hospital cardiac arrest, to analyze the result of CPR, and to evaluate the problems associated with in-hospital CPR. METHODS: A clinical analysis of 71 cases of in-hospital CPR announcement from January 2000 to August 2000 was performed. The initial rhythm on cardiac arrest, return of spontaneous circulation(ROSC), and the survivals were analyzed in the case of the 46 true cardiac arrest patients. RESULTS: During 8 months, there were 71 cases of in-hospital CPR announcement. Among them, there were 46 cases of true cardiac arrest and 25 cases of non-cardiac arrest. Of the 46 true cardiac-arrest cases, 27(58.7%) experienced ROSC, 15(32.6) survived for over 24 hours, and 7(15.2%) survived to be discharged. The initial rhythms on cardiac arrest were 30 cases(65.2%) of asystole, 14(30.4%) of PEA(pulseless electrical activity), and 2(4.3%) of ventricular fibrillation, with ROSC being 17 cases(56.7%), 9(64.3%) and 1(50.0%) cases and discharged survivors being 4 cases(13.3%), 3(21.4%) and 0(0.0%) cases, respectively. CONCLUSION: Extraordinarily high proportions of asystole and PEA were seen in the initial rhythm of cardiac arrest, and those were associated with high survival rates. Although further study is needed to evaluate the course leading to this high proportion of asystole and PEA, this result suggests that if the EMS system in the hospital is activated promptly and systematically, a better outcome will be achieved in case of cardiac arrest with asystole and PEA.
Cardiopulmonary Resuscitation* ; Emergency Service, Hospital ; Heart Arrest ; Humans ; Peas ; Survival Rate ; Survivors ; Tertiary Care Centers* ; Ventricular Fibrillation

BACKGROUND: The continuous infraclavicular brachial plexus block (BPB) has many merits compared to other approaches. However, due to complications and the discomfort felt by patients during the procedure, it has not gained much in popularity. We assumed that the neurovascular sheath is one compartment and placed the catheter deeply into the sheath, as used in the infraclavicular approach, through the axilla. METHODS: Patients scheduled for surgery were paired according to their diagnoses and sites of surgery. Thirty-two patients were randomly chosen and divided into two groups. Selander's continuous axillary BPB was performed in the axillary group. In the infraclavicular group, we inserted an epidural catheter with a stylet deeper into the site just medial to the coracoid process using a C-arm and nerve stimulator. RESULTS: In the infraclavicular group, sensory and motor block of the musculocutaneous nerve and the quality of BPB was superior to those of the axillary group (P < 0.05). The average depth of the catheter from the skin was 14 +/-1.5 cm. CONCLUSIONS: Continuous infraclavicular BPB can cause no more discomfort as Selander's continuous axillary approach. Furthermore, it may improve the quality of block and reduce the amount of local anesthetic used.
Axilla* ; Brachial Plexus* ; Catheters* ; Diagnosis ; Humans ; Musculocutaneous Nerve ; Skin

PURPOSE: The tumor suppressor gene PTEN is located on chromosome 10q23 and is mutated in a variety of sporadic cancers. The aim of this study is to understand the expression of the PTEN gene product in invasive breast cancer and its relationships with clinicopathologic factors of breast cancer. METHODS: The hospital records and pathologic findings of 57 patients who underwent breast operation due to invasive breast carcinoma between January 1996 and June 2002 were reviewed. We examined the relationships between PTEN expression and various clinicopathologic factors of breast cancer such as age, tumor size, lymph node metastasis, histologic grade, nuclear grade, stage and estrogen, and progesterone receptors. RESULTS: Among the 57 invasive breast cancers, 5 (8.8%) were immunohistochemically negative, and 14 (24.6%) had reduced expression of PTEN. Among clinicopathologic factors, age, tumor size, lymph node metastasis, stage, histologic grade, and estrogen receptor had no correlation with PTEN expression. Nuclear grade and positive progesterone receptor were significant correlated with decreased PTEN expression. However, these results are different from those of other reports showing that negative progesterone receptor was significantly correlated with the decrease of PTEN expression. CONCLUSION: According to other reports, PTEN expression does play some role as a prognostic factor for breast cancer, but the results of our study did not support this hypothesis. Further evaluation and study are required into the role of PTEN as a prognostic factor of breast cancer.
Age Factors ; Breast ; Breast Neoplasms* ; Estrogens ; Genes, Tumor Suppressor ; Hospital Records ; Humans ; Lymph Nodes ; Neoplasm Metastasis ; Receptors, Progesterone

PURPOSE: The tumor suppressor gene PTEN is located on chromosome 10q23 and is mutated in a variety of sporadic cancers. The aim of this study is to understand the expression of the PTEN gene product in invasive breast cancer and its relationships with clinicopathologic factors of breast cancer. METHODS: The hospital records and pathologic findings of 57 patients who underwent breast operation due to invasive breast carcinoma between January 1996 and June 2002 were reviewed. We examined the relationships between PTEN expression and various clinicopathologic factors of breast cancer such as age, tumor size, lymph node metastasis, histologic grade, nuclear grade, stage and estrogen, and progesterone receptors. RESULTS: Among the 57 invasive breast cancers, 5 (8.8%) were immunohistochemically negative, and 14 (24.6%) had reduced expression of PTEN. Among clinicopathologic factors, age, tumor size, lymph node metastasis, stage, histologic grade, and estrogen receptor had no correlation with PTEN expression. Nuclear grade and positive progesterone receptor were significant correlated with decreased PTEN expression. However, these results are different from those of other reports showing that negative progesterone receptor was significantly correlated with the decrease of PTEN expression. CONCLUSION: According to other reports, PTEN expression does play some role as a prognostic factor for breast cancer, but the results of our study did not support this hypothesis. Further evaluation and study are required into the role of PTEN as a prognostic factor of breast cancer.
Age Factors ; Breast ; Breast Neoplasms* ; Estrogens ; Genes, Tumor Suppressor ; Hospital Records ; Humans ; Lymph Nodes ; Neoplasm Metastasis ; Receptors, Progesterone