Adenoma malignum (minimal deviation adenocarcinoma) is an extremely well-differentiated adenocarcinoma of uterine cervix. Despite its benign histological appearance, this tumor is thought to be malignant and has a poor prognosis. Because Pap smear and punch biopsy have low sensitivity, accurate diagnosis of the tumor may be difficult. If it is diagnosed, it must be treated same as in cervical cancer. We presented one case of preoperatively diagnosed adenoma malignum by deep cone biposy with a brief review of literature.
Adenocarcinoma ; Adenoma* ; Biopsy ; Cervix Uteri* ; Diagnosis ; Female ; Prognosis ; Uterine Cervical Neoplasms

OBJECTIVE: To evaluate the distribution of HPV subtype and multiple HPV infection in cervical cancer and precancerous lesion with HPV DNA chip in Korean women. METHODS: We recruited a total of 145 women diagnosed with cervical cancer or cervical intraepithelial neoplasia (CIN), and 404 normal women as control between Dec. 2004 and Sept. 2005. We investigated the distribution of HPV subtype and the multiple infection according to cervical lesion. RESULTS: Of the 145 women, 132 (91.0%) showed HPV infection and 111 (76.6%) showed high risk HPV infection by HPV DNA chip. HPV 16 (48/145, 33.1%) was the most common type, and followed by HPV 58, 18, and 33 in CIN or cervical cancer than control (p<0.05). Multiple infections were more common in CIN or cervical cancer than control. There was no correlation between the incidence of multiple HPV infection and cervical lesion in young women (<35 years). In women over 36 years of age, however, multiple infections were the most frequently detected in CIN. CONCLUSION: The types of HPV 16, 58, 18, 33 were common in cervical precancerous or cancer lesion by order of frequency. Multiple infections were common in women under 35 years of age, and in women with CIN over 36 years of age.
Cervical Intraepithelial Neoplasia ; Female ; Human papillomavirus 16 ; Humans ; Incidence ; Oligonucleotide Array Sequence Analysis ; Uterine Cervical Neoplasms*

We describe a case of diamniotic dichorionic pregnancy at 23 weeks and 6 days of gestation with threatened preterm labor with rupture of membrane of twin one. After 10 days we delivered the first twin. We decided to retain the other one to allow improvement in the outcome for the second twin. The patient was treated with tocolytics, antibiotics, cervical cerclage, steroid and continously monitored. After 58 daysthere was increasing uterine contractility and we delivered the second twin.
Anti-Bacterial Agents ; Cerclage, Cervical ; Female ; Humans ; Membranes ; Obstetric Labor, Premature ; Pregnancy ; Pregnancy, Multiple ; Rupture ; Tocolytic Agents ; Twins*

BACKGROUND: The pathophysiologic mechanisms of early acute lung injury (ALI) differ according to the type of primary insult. It is important to differentiate between direct and indirect pathophysiologic pathways, and this may influence the approach to treatment strategies. NF-kappa B decoy oligodeoxynucleotide (ODN) is a useful tool for the blockade of the expression of NF-kappa B-dependent proinflammatory mediators and has been reported to be effective in indirect ALI. The purpose of this study was to investigate the effect of NF-kappa B decoy ODN in the lipopolysaccharide (LPS)-induced direct ALI model. METHODS: Five-week-old specific pathogen-free male BALB/c mice were used for the experiment. In the preliminary studies, tumor necrosis factor (TNF)-alpha, interleukine (IL)-6 and NF-kappa B activity peaked at 6 hours after LPS administration. Myeloperoxidase (MPO) activity and ALI score were highest at 36 and 48 hours, respectively. Therefore, it was decided to measure each parameter at the time of its highest level. The study mice were randomly divided into three experimental groups: (1) control group which was administered 50 microliter of saline and treated with intratracheal administration of 200 microliter DW containing only hemagglutinating virus of Japan (HVJ) vector (n=24); (2) LPS group in which LPS-induced ALI mice were treated with intratracheal administration of 200 microliter DW containing only HVJ vector (n=24); (3) LPS+ODN group in which LPS-induced ALI mice were treated with intratracheal administration of 200 microliter DW containing 160 microgram of NF-kappa B decoy ODN and HVJ vector (n=24). Each group was subdivided into four experimental subgroups: (1) tissue subgroup for histopathological examination for ALI at 48 hours (n=6); (2) 6-hour bronchoalveolar lavage (BAL) subgroup for measurement of TNF-alpha and IL-6 in BAL fluid (BALF) (n=6); (3) 36-hour BAL subgroup for MPO activity assays in BALF (n=6); and (4) tissue homogenate subgroup for measurement of NF-kappa B activity in lung tissue homogenates at 6 hours (n=6). RESULTS: NF-kappa B decoy ODN treatment significantly decreased NF-kappa B activity in lung tissues. However, it failed to improve the parameters of LPS-induced direct ALI, including the concentrations of tumor necrosis factor-alpha and interleukin-6 in BALF, myeloperoxidase activity in BALF and histopathologic changes measured by the ALI score. CONCLUSION: NF-kappa B decoy ODN, which has been proven to be effective in indirect models, had no effect in the direct ALI model.
Acute Lung Injury ; Animals ; Bronchoalveolar Lavage ; Humans ; Inflammation ; Interleukin-6 ; Interleukins ; Lipopolysaccharides ; Lung ; Male ; Mice ; NF-kappa B ; Oligodeoxyribonucleotides ; Peroxidase ; Sendai virus ; Tumor Necrosis Factor-alpha