1.Functional & anatomic reconstruction of the donor site after the transverse rectus abdominis myocutaneous(TRAM) flap transfer.
Jeong Seob YOON ; Hur Bum LEE ; Sang Heon LEE ; Keuk Shun SHIN
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1992;19(3):459-469
No abstract available.
Humans
;
Rectus Abdominis*
;
Tissue Donors*
2.Expression and correlation of TGF-beta1, iNOS, IFN-gamma, and IL-10 mRNA in borderline lepromatous leprosy lesions before and after treatment.
Jeong Hyun SHIN ; Hae Young CHOI ; Ki Bum MYUNG ; Sang Nae CHO
Korean Leprosy Bulletin 2001;34(1):25-46
Leprosy is a chronic granulomatous disease caused by Mycobacterium leprae, which is an obligate intracelluar pathogen. It presents broad spectrum of clinical manifestations depending on the host's specific cell-mediated immune response to M. leprae. Especially, type I Th cells and macrophages are important in defense mechanism to M. leprae, and the immune response is regulated by cytokines secreted by immune cells. Recent investigations showed nitric oxide(NO) was the key molecule in the killing activity of macrophages, which was enhanced by IFN-gamma but suppressed by TGF-beta1 and IL-10. Since cytokine is secreted by activated immune cells with antigenic stimulation, decreased antigens by treatment modulates the expression of cytokines in leprosy. In this study, we observed the dynamics of cytokines expression using RT-PCR, such as TGF-beta1 and IL-10, which suppress the activity of macrophages, IFN-gamma, which activates macrophages, and iNOS, which represents the killing activity of macrophages, in the lesions taken from fifteen borderline lepromatous leprosy patients before and after multiple drug therapy for 4 weeks. The results are summarized as follows: 1. Before treatment, cytokines were expressed in order of IL-10, iNOS, TGF-beta1 and IFN-gamma(p>0.05). 2. After 4 weeks treatment, cytokines were expressed in order of iNOS, IL-10, TGF-beta1 and IFN-gamma(p<0.05). 3. Fifty-four percent of patients showed a non-polarized Th 0 pattern, 33% a polarized Th 1 pattern, and 20% Th-negative. Th 2 pattern was not observed. 4. The changes of cytokines expression after 4 weeks treatment were not significant, although mRNA of IL-10, TGF-beta1 and IFN-gamma were somewhat decreased. 5. There was negative correlation between TGF-beta1 and iNOS(gamma(2)=0.499, p<0.05, before treatment), positive correlation between TGF-beta1 and IFN-gamma(gamma(2)= 0.622, p<0.05, before treatment), and positive correlation between IFN-gamma and IL-10(gamma(2)= 0.935, p<0.05, before treatment; gamma(2)= 0.937, p<0.05, after treatment). In conclusion, these results suggest that TGF-beta1 and IL-10 may contribute to immune suppression in multibacterial leprosy patients, and that TGF-beta1 suppresses iNOS expression in macrophages. With 4 weeks treatment, the significant changes in cytokines expression were not observed. Interestingly, the majority of BL patients showed Th 0 pattern of cytokine, and none of Th 2 pattern.
Cytokines
;
Drug Therapy
;
Granulomatous Disease, Chronic
;
Homicide
;
Humans
;
Interleukin-10*
;
Leprosy*
;
Leprosy, Multibacillary*
;
Macrophages
;
Mycobacterium leprae
;
RNA, Messenger*
;
Transforming Growth Factor beta1*
3.Clinical Features of Cataract Extraction with Negative-Power Intraocular Lens Implantation in High Myopia.
Min Kyo KIM ; Woo Beom SHIN ; Hyun Kyo JEONG ; Jung Bum CHOI
Journal of the Korean Ophthalmological Society 2016;57(6):898-904
PURPOSE: To evaluate the clinical features of high myopic eyes with cataracts implanted with negative power intraocular lenses (IOLs) at the time of cataract surgery. METHODS: A retrospective chart review was conducted of 18 eyes of 14 patients who underwent cataract surgery with negative power IOLs and 10 eyes in 9 patients with low power IOLs. We investigated axial length, IOL power, preoperative and postoperative best-corrected visual acuity (BCVA) and preoperative and postoperative spherical equivalent (SE) refractive errors. RESULTS: Mean BCVA showed significant improvement in both groups. We measured postoperative SE refraction and the difference between the mean intended and the mean achieved SE refractive errors in the negative power group (17 eyes) was +1.59 ± 1.34 D and +0.31 ± 0.50 D in the low power group. CONCLUSIONS: BCVA was significantly improved in the majority of eyes, although they had myopic macular degeneration or posterior staphyloma. However, the mean achieved postoperative SE refraction was more hyperopic than the predicted postoperative SE error. Additionally, hyperopic refractive error was greater in the negative power group than the low power group. Therefore, we recommend that postoperative hyperopic refractive error should be considered when performing cataract surgery in high myopic patients.
Cataract Extraction*
;
Cataract*
;
Humans
;
Lens Implantation, Intraocular*
;
Lenses, Intraocular*
;
Macular Degeneration
;
Myopia*
;
Refractive Errors
;
Retrospective Studies
;
Visual Acuity
4.A Case of Atypical Acquired Reactive Perforating Collagenosis.
Seong Hyun KIM ; Jeong Hyun SHIN ; Hae Young CHOI ; Ki Bum MYOUNG
Korean Journal of Dermatology 2001;39(10):1133-1137
Reactive perforating collagenosis(RPC) is a kind of perforating dermatosis characterized by transepidermal elimination of altered dermal collagen. RPC is classified into two forms; childhood or inherited form, and adulthood or acquired form. Acquired RPC is reported to occur in association with the severe complicated diabetes mellitus, chronic renal failure and other diseases. We describe a 43-year-old Korean woman with atypical acquired RPC associated with uncontrolled diabetes mellitus and severe pruritus. The histopathologic findings of the lesions showed neither transepidermal channel nor cup-shaped epidermal depression. Multiple degenerated collagen bundles arranged vertically and were eliminated through epidermis to the surface individually. Transmission electron microscopic findings showed the same as typical RPC. Skin lesions improved after the insulin subcutaneous injections, UVB phototherapy and antihistamine administration.
Adult
;
Collagen
;
Depression
;
Diabetes Mellitus
;
Epidermis
;
Female
;
Humans
;
Injections, Subcutaneous
;
Insulin
;
Kidney Failure, Chronic
;
Phototherapy
;
Pruritus
;
Skin
;
Skin Diseases
5.Oculogyric Crisis after Albendazole Medication.
Du Shin JEONG ; Sang Gull CHO ; Moo Young AHN ; Ki Bum SUNG
Journal of the Korean Neurological Association 1998;16(3):421-422
An oculogyric crisis which was resolved after intravenous injection of diazepam 10 mg developed in a 16-year-old girl after an oral dose of 400 mg of albendazole. Although ocular side effects of albendazole are rare, it can induce an alarming oculogyric crisis in some sensitive subjects. The mechanism of the oculogyric crisis is unclear.
Adolescent
;
Albendazole*
;
Diazepam
;
Female
;
Humans
;
Injections, Intravenous
6.Sphingosine 1-Phosphate Triggers Apoptotic Signal for B16 Melanoma Cells via ERK and Caspase Activation.
Jeong Hyun SHIN ; Gwang Seong CHOI ; Won Hyung KANG ; Ki Bum MYUNG
Journal of Korean Medical Science 2007;22(2):298-304
The bioactive sphingolipid metabolite sphingosine 1-phosphate (S1P), recently was reported to induce apoptosis of some cancer cells and neurons, although it generally known to exert mitogenic and antiapoptotic effects. In this study, we investigated the effects of S1P on the cell growth, melanogenesis, and apoptosis of cultured B16 mouse melanoma cells. In results, S1P was found to induce apoptosis in B16 melanoma cells in a dose- and time-dependent manner, but exerted minimal effects on melanogenesis. Although receptors of sphingosine 1-phosphate (endothelial differentiation gene 1 [Edg]/S1P1, Edg5/S1P2, Edg3/S1P3) were expressed in B16 melanoma cells, they were shown not to be associated with S1P-induced apoptosis. In addition, pertussis toxin did not block the apoptotic effects of S1P on B16 melanoma cells. S1P induced caspase-3 activation and the extracellular signal-regulated kinase (ERK) activation. Interestingly, the ERK pathway inhibitor, UO126, reversed the apoptotic effects of S1P on B16 melanoma cells. These results suggest that S1P induced apoptosis of B16 melanoma cells via an Edg receptor-independent, pertussis toxin-insensitive pathway, and appears to be associated with the ERK and caspase-3 activation.
Sphingosine/administration & dosage/*analogs & derivatives
;
Signal Transduction/drug effects
;
Mice
;
Melanoma/*enzymology/*pathology
;
Lysophospholipids/*administration & dosage
;
Extracellular Signal-Regulated MAP Kinases/*metabolism
;
Enzyme Activation/drug effects
;
Cell Line
;
Caspase 3/*metabolism
;
Apoptosis/*drug effects
;
Animals
7.Echocardiographic Diagnosis of Left Ventricular Hypertrophy.
Chang Bum KIM ; Yoon Mo YANG ; Chang Seoup SHIN ; Jong In LEE ; Dae Ha KIM ; Jeong Wun HWANG
Korean Circulation Journal 1982;12(2):157-165
Echocardiography was done on 51 cases with various cardiovascular disease and on 23 cases of normal control subjects from April, 1981 to March, 1982 in the Department of internal medicine, Eul Ji General Hospital, Seoul, Korea. The results obtained were as follows: 1. The left ventricular mass estimated by the measurements made with standard convention was 261+/-8gm in patient group and 126+/-6gm in control group. With penn convention, the left ventricular mass of the patients group was 297+/-11gm and that of control group was 127+/-6gm. The differences between both conventions were significant statistically. 2. In measurements made with standard convention, those who showed increased left ventricular posterior wall thickness were 37 cases(73%) and those who showed increased left ventricular internal dimension were 15 cases(29%). On the other hand, number of cases who showed increased left ventricular mass were 45 cases(88%) and this results suggested estimation of left ventricular mass seems to be more useful method in the diagnosis of left ventricular hypertrophy than simple measurements of left ventricular posterior wall thickness of left ventricular internal dimension. 3. The correlation coefficient between left ventricular mass and maximum voltage of electrocardiography, left ventricular posterior wall thickness left ventricularinternal dimension was 0.70, 0.74 0.51 respectively in standard method and 0.82, 0.76, 0.44 respectively in penn convention. Left ventricular mass was correlated fairly closely with the maximum voltage of electrocardiography and left ventricular posterior wall thickeness, less closely with the left ventricular internal dimension.
Cardiovascular Diseases
;
Diagnosis*
;
Echocardiography*
;
Electrocardiography
;
Hand
;
Hospitals, General
;
Humans
;
Hypertrophy, Left Ventricular*
;
Internal Medicine
;
Korea
;
Seoul
8.Serial Brain CT Scans in Severe Head Injury without Intracranial Pressure Monitoring.
Dong Seong SHIN ; Sun Chul HWANG ; Bum Tae KIM ; Je Hoon JEONG ; Soo Bin IM ; Won Han SHIN
Korean Journal of Neurotrauma 2014;10(1):26-30
OBJECTIVE: The intracranial pathologies after head trauma should be usually progressed. It is clearly visualized in the non-invasive brain CT. The invasive monitor such as intracranial pressure (ICP) monitoring may be accompanied with the complications. This study aims whether the patients with severe head injury could be managed with serial CT scans. METHODS: The medical records of 113 patients with severe head injury in the prospectively enrolled trauma bank were retrospectively analyzed. After the emergency care, all the patients were admitted to the intensive care unit for the aggressive medical managements. Repeat brain CT scans were routinely taken at 6 hours and 48 hours after the trauma. ICP monitoring was restrictively applied for the uncertain intracranial pressure based on the CT. The surgical intervention and the mortality rate were analyzed. RESULTS: Immediate surgical intervention after the initial CT scan was done in 47 patients. Among the initially non-surgical patients, 59 patients were managed with the serial CT scans and 7 with the ICP monitoring. Surgical interventions underwent eventually for 10 patients in the initially non-surgical patients; 1 in the ICP monitoring and 9 in the serial CT. The mortality rate was 23.7% in the serial brain CT and 28.6% in the ICP monitoring. There was no statistical difference between two groups in the aspect of mortality (p=0.33). CONCLUSION: Serial CT scans in time could be a good way to monitor the intracranial progression in the severe head injury and reduce the implantation of an invasive ICP probe.
Brain*
;
Craniocerebral Trauma*
;
Emergency Medical Services
;
Humans
;
Intensive Care Units
;
Intracranial Pressure*
;
Medical Records
;
Mortality
;
Pathology
;
Prospective Studies
;
Retrospective Studies
;
Tomography, X-Ray Computed*
9.Influence of Ischemic-Simulation on the Action Potential Characteristics in Rat Atrial Fibers.
Jae Ha KIM ; Jeong Min JU ; Yong Bum CHO ; Dong Ho SHIN ; Byung Hee AHN ; Shin Bae JOO ; Sang Hyung KIM
Korean Circulation Journal 1999;29(11):1225-1233
BACKGROUND: To investigate the mechanisms of myocardial ischemia induced changes of electrophysiological properties, influences of various ischemic-simulated Tyrode's solutions on the changes of action potential characteristics were examined. METHOD: Action potential characteristics were measured during superfusion with various ischemic-simulated solutions (modified physiologic salt solution: MPSS) by the method of conventional microelectrode technique in rat atrial fibers. RESULTS: Hypoxic-, hyperkalemic-, and mixed-MPSS decreased 'maximum diastolic potential' (MDP) and 'action potential amplitude' (APA), however, no significant changes of MDP and APA were observed by acidic- and glucose-free-MPSS. 'Maximum velocity of phase 0 depolarization' (dV/dt(max)) and 'time for 90% repolarization' (APD90) significantly decreased during hypoxic- and mixed-MPSS superfusion, and hyperkalemic-MPSS also decreased the dV/dt(max) and APD90. However, no significant changes in dV/dt(max) and APD90 were observed by acidic- and glucose-free-MPSS. The decreasing effects of dV/dt(max) and APD90 by the MPSSes were attenuated when the MPSSes were replaced with normal Tyrode's solution. DPCPX (2x10(-6)M), a purinergic antagonist, inhibited the decreasing effects of APD90 at 5, 10, and 20 min superfusion of the mixed-MPSS, and glibenclamide (10(-6)M), a K(ATP) channel blocker, inhibited those at 10 and 20 min superfusion of the mixed-MPSS. Diclofenac (10(-6)M), a cyclooxygenase inhibitor inhibited only those at 20 min superfusion of the mixed-MPSS. CONCLUSION: The primary factors for changing the electrophysiological characteristics during ischemic insults could be hypoxia and high-extracellular K+, and the mechanisms of the electrophysiological changes are inferred that adenosine through purinoceptors is involved initially, and followed by K(ATP) channel and prostanoids.
Action Potentials*
;
Adenosine
;
Animals
;
Anoxia
;
Diclofenac
;
Glyburide
;
Ischemia
;
Microelectrodes
;
Myocardial Ischemia
;
Prostaglandin-Endoperoxide Synthases
;
Prostaglandins
;
Rats*
;
Receptors, Purinergic
10.A Pilot Study on Total Plasma Homocysteine Level of Patients with Cerebral Infarction.
Du Shin JEONG ; In Uk SONG ; Sang Gull CHO ; Ki Bum SUNG ; Hyung Kook PARK ; Hyun Kil SHIN ; Sung Geun JANG
Journal of the Korean Neurological Association 1999;17(1):26-31
BACKGROUND: Hyperhomocysteinemia has been known to be associated with cerebrovascular disease. It is toxic to vascular endothelial cell and promotes arteriosclerotic detachment. There was no study of the homocysteine level in ischemic stroke patients in Korea except case report. Therefore we measured total plasma homocysteine level in ischemic stroke patients and evaluated association with other stroke risk factors. METHODS: We measured total plasma homocysteine level in 44 patients with ischemic stroke and 16 age-matched normal controls, and analyzed its relationship to other stroke risk factors. Total plasma homocysteine level was determined by using a radioenzymatic method. RESULTS: Total plasma homocysteine level was 12.12 5.11 micromol/L in patients and 9.40 1.93 micromol/L in controls. It was 13.03 5.78 micromol/L in lacunar infarction and 9.96 1.57 micromol/L in territory infarction. The total plasma homocysteine level of the patients with hypertension, DM, hyperlipidemia, heart disease, smoking, alcohol, previous stroke, carotid bruit was not different statistically from those without them. CONCLUSIONS: Total plasma homocysteine level was significantly higher in the ischemic stroke patients than that of controls. Lacunar infarction showed significantly higher level of total plasma homocysteine compared to territory infarction.
Cerebral Infarction*
;
Endothelial Cells
;
Heart Diseases
;
Homocysteine*
;
Humans
;
Hyperhomocysteinemia
;
Hyperlipidemias
;
Hypertension
;
Infarction
;
Korea
;
Pilot Projects*
;
Plasma*
;
Risk Factors
;
Smoke
;
Smoking
;
Stroke
;
Stroke, Lacunar