No abstract available.
Humans ; Male ; Military Personnel*

No abstract available.
Humans ; Male ; Military Personnel*

No abstract available.
Arteries* ; Heart*

No abstract available.
DNA, Viral* ; Hep G2 Cells* ; Hepatitis B virus* ; Hepatitis B* ; Hepatitis* ; Spices*

To investigate effects of cytokines on rheumatoid synovial cells, proliferation and expression of cytokine and metalloproteinase genes were studied with the primary culture of rheumatoid synovial cells which was treated with TNF-alpha, GM-CSF, TGF-alpha, PDGF and IL-B. By [3H] thymidine incorporation assay, TGF-beta and PDGF increased proliferation of synovial cells by 1.5 and 2.5 folds respectively. Cytokine gene expression was assessed by RT-PCR. Rheumatoid synovial cells expressed constitutively TGF-beta and IL-B at a high level and IL-1B, GM-CSF, and MIP-1a at a relatively low level. TGF-beta, GM-CSF and PDGF increased IL-B expression. Expression of pro-inflammatory cytokines and chemokines was increased by GM-CSF and PDGF. Both GM-CSF and PDGF increased the expression of IL-1B, GM-CSF MIP-la and IL-8. In addition, GM-CSF enhanced expression of TNF-alpha. Stromelysin and collagenase are the major proteinases responsible for destruction ot joints in rheumatoid arthritis (RA). These genes were expressed constitutivefy in rheumatoid synovial cells. In summary, PDGF and GM-CSF may piay an important role by inducing or increasing expression of IL-1B, TGF-beta and PDGF by increasing proliferation of rheumatoid synovial cells.
Tumor Necrosis Factor-alpha

This studly was perormed 10 define the role of compulerized quantitative analysis in ewalualion for overexpression of p53 by immunohistochemistry. Total and ladeled cells wers counted automatically using commercially available software for color-image analysis. In 16 uterine cerwical carcinomas, the p53 ladeling index calculted bycomputerized5quantitatiwe analysis was 9.22%+/-8.70% and by visual analysis 5.90+/-6.51%. The present results suggest that the computerized quantitative analysis may be valuadle in objective interetation of immunohistochemical expression of p53 and reliable than conventional ways of visual analysis.
Cervix Uteri* ; Female ; Immunohistochemistry

Extracorporeal shock wave lithotripsy (ESWL) has proved to be an effective method of treating upper urinary tract calculi in adults. But the application of ESWL in children was less clearly defined. We report on 28 pediatric patients who were treated by ESWL with the Northgate SD-3 lithotriptor between June, 1989 and May, 1994. The patient age ranged from 2 years to 17 years, with an average age of 11.1 years. The conditions known to be associated with stone formation were urinary tract infection in 6 patients (21.4%), vesicoureteral reflux in 1 patient (3.6%) and unknown in other patients. The total average success rate of treatment was 92.9%. ESWL complications required admission and surgical management were not observed. We concluded that ESWL with the Northgate SD-3 lithotriptor is a safe and effective method of treating upper urinary tract calculi in children.
Adult ; Calculi ; Child* ; Humans ; Lithotripsy* ; Shock* ; Urinary Tract ; Urinary Tract Infections ; Vesico-Ureteral Reflux

Although a variety of agents have been introduced for the treatment of hypertension, the ideal drug has not yet been discovered. However, among the agents available, prazosin hydrochloride (Minipress(R)) appears to be accepted by the majority of physicians as it lowers blood pressure effectively with relatively low incidence of side effects. It has been considered that the hypotensive effect of prazosin is a result of peripheral vasodilation due to direct relaxation of vascular smooth muscle and blockade of alpha-adrenergic receptors in the arteriolar smooth muscle. Following the development of prazosin. many trials have been designed to potentiate its hypotensive effect by the combination with other agent, especially thiazide or beta blocker. Minizide(R), a preparation that thiazide is added to prazosin, is an example. The antihypertensive effect of Minizide(R) was evaluated in 30 hypertensive subjecs. The results were as follows; 1. The systolic and diastolic blood pressure were effectively lowered in both supine and standing position with the overall response rate of 86.7% as judged to be responsive in one that lost systolic pressure more than 15 mmHg and distolic pressure 10 mmhg. Among 30 cases 18 cases could be maintained with their diastolic pressure below 90 mmHg and 24 cases with their diastolic pressure below 100 mmHg by the end of 8th week of treatment with the daily dose of minizide(R) from a half tablet to two tablets. 2. During the period of 8 weeks, troublesome postural hypotension was not observed in any case. The pulse rate was not accelerated significantly by Minizide(R) treatment. 3. Minimal side effects were recorded in 5 cases; two of mild nausea, one of slight dizziness, one of minimal gastrointestinal irritation symptom and one of mild glucosuria. neither of them hindered the authors from finding the study.
Blood Pressure ; Dizziness ; Heart Rate ; Hypertension ; Hypotension, Orthostatic ; Incidence ; Muscle, Smooth ; Muscle, Smooth, Vascular ; Nausea ; Prazosin ; Receptors, Adrenergic, alpha ; Relaxation ; Tablets ; Vasodilation

Although a variety of agents have been introduced for the treatment of hypertension, the ideal drug has not yet been discovered. However, among the agents available, prazosin hydrochloride (Minipress(R)) appears to be accepted by the majority of physicians as it lowers blood pressure effectively with relatively low incidence of side effects. It has been considered that the hypotensive effect of prazosin is a result of peripheral vasodilation due to direct relaxation of vascular smooth muscle and blockade of alpha-adrenergic receptors in the arteriolar smooth muscle. Following the development of prazosin. many trials have been designed to potentiate its hypotensive effect by the combination with other agent, especially thiazide or beta blocker. Minizide(R), a preparation that thiazide is added to prazosin, is an example. The antihypertensive effect of Minizide(R) was evaluated in 30 hypertensive subjecs. The results were as follows; 1. The systolic and diastolic blood pressure were effectively lowered in both supine and standing position with the overall response rate of 86.7% as judged to be responsive in one that lost systolic pressure more than 15 mmHg and distolic pressure 10 mmhg. Among 30 cases 18 cases could be maintained with their diastolic pressure below 90 mmHg and 24 cases with their diastolic pressure below 100 mmHg by the end of 8th week of treatment with the daily dose of minizide(R) from a half tablet to two tablets. 2. During the period of 8 weeks, troublesome postural hypotension was not observed in any case. The pulse rate was not accelerated significantly by Minizide(R) treatment. 3. Minimal side effects were recorded in 5 cases; two of mild nausea, one of slight dizziness, one of minimal gastrointestinal irritation symptom and one of mild glucosuria. neither of them hindered the authors from finding the study.
Blood Pressure ; Dizziness ; Heart Rate ; Hypertension ; Hypotension, Orthostatic ; Incidence ; Muscle, Smooth ; Muscle, Smooth, Vascular ; Nausea ; Prazosin ; Receptors, Adrenergic, alpha ; Relaxation ; Tablets ; Vasodilation

The investigation of systolic time intervals and hemodynamics was performed in 42 patients with liver cirrhosis by noninvascive methods. The patients were divided into four groups according to the presence or absence of anemia and/or ascites: i.e. group I; cirrhosis without anemia and ascites, group II; cirrhosis with ascites only, group III; cirrhosis with anemia only, and group IV; cirrhosis with ascites and anemia. In the resting state of the patients, the systolic time intervals and hemodynamic data were measured by the high speed simultaneous recordings of electrocardiogram, phonocardiogram, carotid and femoral arterial pulse tracings, and compared with those obtained from 155 normal adult subjects. 1. The pulse were increased considerably in group IV, and diastolic blood pressure was elevated in group II with significance. 2. The stroke volume and cardiac output were increased significantly in group III. 3. The peripheral resistance was reduced particularly in group III, and the volume elasticit coefficient was decreased in group IV. 4. The QS1 interval was prolonged significantly in group II and IV, but QS1 interval corrected by multiple regression equation proposed by our laboratory (illustrated in the text) did not show significant difference compared with that of normal subjects. 5. The left ventricular ejection time(LVET) and total electromechanical systole(QS 2) appeared to be shortened in group II and IV, but the corrected LVET and QS 2 were not different significantly from those of the normal subjects
Adult ; Anemia ; Ascites ; Blood Pressure ; Cardiac Output ; Electrocardiography ; Fibrosis ; Hemodynamics* ; Humans ; Liver Cirrhosis* ; Liver* ; Stroke Volume ; Systole ; Vascular Resistance