No abstract available.
Child* ; Humans

No abstract available.
Crowns*

The retinoblastoma (Rb)/cyclin D1/p16 pathway is an important constituent of cell cycle regulation. Perturbations in this pathway due to a variety of genetic aberrations have been reported in many human cancers including breast cancer. We examined the significance of immunoexpression of p16 protein, cyclin D1 protein, Rb protein (pRb), and p53 protein in 128 cases of invasive breast carcinoma. The results were correlated with survival rate and clinicopathological variables, including age, histologic grade, lymph node status, tumor size, estrogen receptor (ER), and progesterone receptor (PR) content. Abnormal expressions of p16 and pRb which were defined as negative staining were seen in 21% and 43% of tumors, respectively. There was a significant inverse relationship between p16 and pRb expression. There was no correlation between p16 staining and any other parameters, including survival rate, cyclin D1, p53, and clinicopathologic variables. Surprisingly, there was a trend for tumors which were positive for pRb to be grade III ductal carcinomas. Cyclin D1 positivity was noted in 46% of cases. The expression of cyclin D1 protein was significantly higher in lower histologic grade, higher ER and PR expression. The expression of p53 protein showed a significant correlation with high tumor grade. In a Cox multivariate analysis, neither p16, pRb, cyclin D1 nor p53 was an independent predictor, but tumor size and lymph node status were independent predictors of patient outcome.
Breast Neoplasms ; Breast* ; Carcinoma, Ductal* ; Cell Cycle ; Cyclin D1* ; Cyclins* ; Estrogens ; Humans ; Lymph Nodes ; Multivariate Analysis ; Negative Staining ; Receptors, Progesterone ; Retinoblastoma ; Retinoblastoma Protein ; Survival Rate

No abstract available.
Breast Neoplasms* ; Breast* ; Cyclin D1* ; Cyclins* ; Survival Rate*

In this study of 64 cases of breast cancer with a clinical follow-up period of more than 5 years, several prognostic factors were evaluated. The purpose of this study was to determine whether any one parameter or group of parameters serves as adequate predictors of tumor behavior and patient's prognosis. Several prognostic factors included clinicopathological variables (patient's age, histologic grade, status of lymph node (LN) metastasis, and tumor size), expression of estrogen receptor (ER), progesterone receptor (PR), p53, bcl-2 and CD44 by immunohistochemistry, and DNA ploidy pattern. The results showed that the expression of ER and PR had a significant inverse correlation with the histologic grade (ER, p=0.05; PR, p<0.05). The expression of p53 protein showed a significant relationship with high histologic grade of tumor (p<0.05). The expression of bcl-2 protein was preferably seen in low histologic grade of tumor (p<0.05) and significantly associated with ER positive or PR positive tumors (ER, p<0.05; PR, p<0.05). This results suggest that bcl-2 protein might play significant roles in ER and PR. The CD44 expression showed a significant relationship with tumor size (p<0.05). The large size and aneuploidy pattern of tumor had a tendency to be associated with shorter patient survival. Cox's multivariate analysis showed that overall survival was affected by LN metastasis because of the shorter survival in patients with LN metastasis. In conclusion, tumor size, DNA ploidy pattern, and LN metastasis were themselves significant predictors of breast cancer survival rate.
Aneuploidy ; Breast Neoplasms ; Breast* ; Carcinoma, Ductal* ; DNA* ; Estrogens ; Follow-Up Studies ; Humans ; Immunohistochemistry ; Lymph Nodes ; Multivariate Analysis ; Neoplasm Metastasis ; Ploidies* ; Prognosis ; Receptors, Progesterone ; Survival Rate

BACKGROUND: As more than 80% of Korean hospital laboratories don't use glycerol blank for the triglyceride measurement, we investigated free glycerol interference on the measurement of triglyceride concentration. METHODS: We collected 237 specimens which had more than 300mg/dL of triglyceride measured by Abbott (without glycerol blank, USA) reagent from the 217 patients visiting Catholic University Hospital of Taegu Hyosung from September, 1995, to May, 1996. We retested them with Youngdong (without glycerol blank, Korea) reagent, and IRC reagent (with glycerol blank, Japan), and also measured free glycerol concentration with Youngdong reagent. Then we examined the clinical records of the patient showing increased glycerol concentration. RESULTS: Average triglyceride concentration measured by Abbott reagent was 448.0+/- 165.9 mg/dL and average glycerol interference to triglyceride concentration was 3.4+/- 6.7%, There were 8 patients (3.4%) who revealed more than 10 % of glycerol interference. Except these patients the average glycerol interference was 2.4+/- 1.4%. Among these 8 patients, 3 patients were treated with Frucenil and other 3 were with Intralipose(R), and the other 2 were diagnosed as diabetes and preeclampsia respectively. In vitro test, Frucenil mixed with serum in 1% (v/v) increased triglyceride concentration as high as 640.6% with Abbott reagent, but it did only 48.4% with IRC reagent. CONCLUSIONS: Triglyceride measurement by non glycerol blank method exceeded the recommended limit of National Cholesterol Education Program's precision, and this method extremely overestimated the triglyceride concentration for the patient with the glycerol containing fluid therapy. So all laboratories would be encouraged to use the glycerol blank method for triglyceride measurements.
Cholesterol ; Daegu ; Education ; Fluid Therapy ; Glycerol* ; Humans ; Laboratories, Hospital ; Pre-Eclampsia ; Triglycerides*

BACKGROUND: As more than 80% of Korean hospital laboratories don't use glycerol blank for the triglyceride measurement, we investigated free glycerol interference on the measurement of triglyceride concentration. METHODS: We collected 237 specimens which had more than 300mg/dL of triglyceride measured by Abbott (without glycerol blank, USA) reagent from the 217 patients visiting Catholic University Hospital of Taegu Hyosung from September, 1995, to May, 1996. We retested them with Youngdong (without glycerol blank, Korea) reagent, and IRC reagent (with glycerol blank, Japan), and also measured free glycerol concentration with Youngdong reagent. Then we examined the clinical records of the patient showing increased glycerol concentration. RESULTS: Average triglyceride concentration measured by Abbott reagent was 448.0+/- 165.9 mg/dL and average glycerol interference to triglyceride concentration was 3.4+/- 6.7%, There were 8 patients (3.4%) who revealed more than 10 % of glycerol interference. Except these patients the average glycerol interference was 2.4+/- 1.4%. Among these 8 patients, 3 patients were treated with Frucenil and other 3 were with Intralipose(R), and the other 2 were diagnosed as diabetes and preeclampsia respectively. In vitro test, Frucenil mixed with serum in 1% (v/v) increased triglyceride concentration as high as 640.6% with Abbott reagent, but it did only 48.4% with IRC reagent. CONCLUSIONS: Triglyceride measurement by non glycerol blank method exceeded the recommended limit of National Cholesterol Education Program's precision, and this method extremely overestimated the triglyceride concentration for the patient with the glycerol containing fluid therapy. So all laboratories would be encouraged to use the glycerol blank method for triglyceride measurements.
Cholesterol ; Daegu ; Education ; Fluid Therapy ; Glycerol* ; Humans ; Laboratories, Hospital ; Pre-Eclampsia ; Triglycerides*

Lung cancer is the most common malignant tumor worldwide and its incidence continues to rise each year. Recent development of molecular biologic method has led to advances in determining the etiologic factors of lung cancer and the establishment of cell lines has provided a lot of information on the through chemosensitivity, radiation biology studies, cytogenetics, and molecular biologic studies, which permits improved treatment for lung cancer. We established a small cell lung cancer cell line, designated JePa-1, obtained from malignant pericardial effusion of small cell lung cancer patient and characterized its morphologic and molecular biologic features. the JePa-1 cell line grew relatively slowly (doubling time 45hrs) as very loosely adherent floating aggregates growing in small clumps with distinct cell outlines and intertwined cords. Also JePa-1 cell line secreted antidiuretic hormones. Electronmicroscopic examination revealed that JePa-1 cell line and xenografts contained electron dense core granules, characteristic of being of neuroendocrine origin. To investigate the tumorigenic capacity, the JePa-1 cell line was injected into SCID and nude mice. Tumors taken from xenografts were observed in 3 out of 4 of the SCID mice and 2 out of 4 of the nude mice. The histologic characteristics of the xenografts were similar to those of the cell line and the original cytologic finding of the pericardial fluid, suggesting small cell carcinoma. The results of immunohistochemical markers showed reactivity for Rb protein, c-myc, TGF-alpha, TGF-beta , EGFR, keratin, NSE, chromogranin, and EMA. The DNA ploidy and the index of the JePa-1 cells was tetraploid and 2.13, respectively. The positive rate for the Rb, c-myc and K-ras proteins of the JePa-1 cell line were 98.9%, 99.3%, and 99.7% respectively as determined by flow cytometry. Cytogenetic analysis using the G-banding technique showed 65 chromosomes with various numerical and structural abnormalities. On examination of the expression of TGF-alpha, TGF-beta , and EGFR by PCR, only the EGFR was positive Through the establishment of JePa-1 cell line, we report in this paper the characterization of a small cell lung cancer such as morphologic and immunocytochemical features, growth characteristics in culture, hormone production, expression of oncoprotein and several growth factors, tumorigenicity, chromosomal abnormalities, and DNA ploidy and index. The JePa-1 cell line will be valuable in vitro studies for the etiology, treatment and the prognostic factors in small cell lung cancer.
Animals ; Carcinoma, Small Cell ; Cell Line ; Chromosome Aberrations ; Cytogenetic Analysis ; Cytogenetics ; DNA ; Flow Cytometry ; Heterografts ; Humans ; Immunohistochemistry ; Incidence ; Intercellular Signaling Peptides and Proteins ; Lung Neoplasms ; Mice ; Mice, Nude ; Mice, SCID ; Pericardial Effusion ; Ploidies ; Polymerase Chain Reaction ; Radiobiology ; Retinoblastoma Protein ; Small Cell Lung Carcinoma* ; Tetraploidy ; Transforming Growth Factor alpha ; Transforming Growth Factor beta ; Vasopressins

We present a prospective, randomized study of acute burst fracture of the thoracolumbar spine. Forty-one patients were treated either by anterior decompression and stabilization with Kaneda device or by posterior distraction instrumentation using the A-O fixateur interne. The mean follow up was 21 months. The result were as follows; 1. The mean preoperative kyphotic angle was 19.2° in those patients treated by anterior surgery and 21.4° in those patients treated by posterior surgery. At last follow-up the mean correction in kyphotic angle was 13.2° in the anterior group and 9.2° in the posterior group. There is no statistically significant difference between those two groups. 2. The mean preoperative midsagittal diameter of the canal compromise 47.4% in anterior group and 49.3% in posterior group. Postoperatively, this was reduced to 2.5% and 7.9%. There is a statistically significant difference between these two groups(P < 0.05). 3. The mean preoperative canal enchroachment 52.)% in anterior group and 47.6% in posterior group. Postoperatively, this was reduced to 3.2% and 6.0%. There is a statistically significant difference between these two group(P < 0.05). 4. Neurologic recovery was 81% in posterior surgery and 85% in anterior surgery. The improvement in Frankel grade was 1 grade in average, and showed no difference between two groups. 5. There was two cases of pedicle screw breadage in posterior group and one case of pyothorax in anterior group but no early or late vascular or neurologic complication.
Decompression ; Empyema, Pleural ; Follow-Up Studies ; Humans ; Pedicle Screws ; Prospective Studies ; Spine

The authors report the clinical results of ten cases of complex supracondylar fractures treated by external fixation with a mean follow up of 21 months. Six cases were closed comminuted fracture, and four cases were open fractures. By AO classification six cases were type C2, two type C3, one type Al, and one type A3. Four cases had associated vascular injury. Reduction was performed by closed method in eight cases, and two cases were reduced through open wound. All cases healed at average of 142 days. Final healing of all cases occurred with an average range of motion of 3°-119° except one case which required reapplication due to infected nonunion with a gap. In nine cases of the ten functional results were rated as "good", and one case was "fair" by Iowa Knee Rating Scale. In conclusion, definitive external fixation can be a good alternative treatment modality despite previous recommendations to limit external fixation to temporary application in cases of limb salvage, such as severe comminution or pre-existing infection.
Classification ; External Fixators ; Femur ; Follow-Up Studies ; Fractures, Comminuted ; Fractures, Open ; Iowa ; Knee ; Limb Salvage ; Methods ; Range of Motion, Articular ; Vascular System Injuries ; Wounds and Injuries