No abstract available.
Biofilms* ; Porphyromonas gingivalis

The present study was done to evaluate the environmental factors responsible for the expression of Porphyromonas gingivalis heat shock protein. The intensity of the heat shock protein gene expression was comparable to those seen by the heat shock ptreatment of the bacteria (44 degrees C) when the bacteria was grown as a mixed culture or biofilm state at 37degrees C.
Bacteria ; Biofilms ; Gene Expression ; Heat-Shock Proteins* ; Hot Temperature* ; Porphyromonas gingivalis* ; Porphyromonas* ; Shock

No abstract available.
Fusobacterium nucleatum* ; Fusobacterium* ; Immunization* ; Porphyromonas gingivalis* ; Porphyromonas*

No abstract available.
Humans

The present study has been performed to develop a PCR technology to identify human immunoglobulin(Ig) allotypes with restriction fragment length polymorphism(RFLP) using a probe. Genomic DNA were ampilified with PCR tecnology using primers from peripheral blood lymphocytes of 10 periodontal patiens, whose Ig allotypes have been pre-determined by serological tecnique using heagglutination technique. The result indicated that the RFLP patterns could successfully differentiate the Ig allotypes, which suggests that this technology can be developed as a tool useful for population genetics studies.
DNA ; Genetics, Population ; Humans ; Immunoglobulin Allotypes* ; Immunoglobulins* ; Lymphocytes ; Polymerase Chain Reaction* ; Polymorphism, Restriction Fragment Length

The aim of the study was to see the total IgG and IgG subclass responses against Aa and Pg in the four early onset periodontitis (EOP) subforms or adult periodontitis (AP). 6 patients consisting of 3 patients from subform I (distinctive LJP pattern), 19 from subform II (post-juvenile periodontitis pattern), 16 from subform III ( LJP pattern but rapidly progressing), 24 from age-matched AP (20-40 years of age) have been selected for the measurements of the total IgG and each IgG subclass against to Pg and the IgG subclass against Aa, respectively. The total IgG titers against to Pg of the subforms I & III had a significantly higher values than subforms II and IV (P<0.05). Among the IgG subclasses, only the lgG3 levels were significantly higher in the subform I than the subform IV(P <0.05). Wide ranges of the antibody titers were noted in all of the EOP subforms and the AP. Except for the subform I, which was typical of localized form, the IgG2 subclass levels to Pg gradually became higher in accordance with the subforms II, III and IV. Both of IgG2 and the IgG4 antibody levels of the EOP were significantly higher than those of AP, while other subclasses were not. All of the four IgG subclass levels to Pg were consistently found to be higher in the younger age group around 20. The levels found to be low around the thirties and then gradually became higher at the ages of late thirties. The IgG2 titer to Aa in the subform I was significantly higher than those of any other subforms. Combinations of IgG1+2+4 were the most frequently found to be elevated followed by the IgG4 only, the IgG2 only, the IgG2+4, the IgG2+3+4, and the IgG1 only, in the descending order.
Aggregatibacter actinomycetemcomitans ; Aggressive Periodontitis* ; Chronic Periodontitis ; Humans ; Immunoglobulin G* ; Periodontitis ; Porphyromonas gingivalis

No abstract available.
Dental Occlusion ; Dental Restoration, Permanent ; Education, Dental, Continuing ; Dental Care

In the study presented here, identification, purification, and partial characterization of a hemin-regulated protein in Prevotella nigrescens were carried out. The results of this study confirm that the availability of hemin influences the expression of a selected membrane protein as well as the growth rate of P. nigrescens ATCC 33563. The 50 kDa cell envelope associated protein, whose expression is hemin regulated, is considered to be a putative hemin-binding protein from P. nigrescens. Disulfide bonds were not present in this protein, and N'-terminal amino acid sequence analysis revealed that this protein belongs to a new, so far undescribed protein. The 50 kDa protein was found to be rich in hydrophilic amino acids, with glycine comprising approximately 60% of the total amino acids. The study described here is the first to identify, purify, and biochemically characterize a putative hemin-binding protein from P. nigrescens. Work is in progress to further characterize the molecular structure of this protein.
Amino Acids ; Glycine ; Hemin ; Membrane Proteins ; Molecular Structure ; Prevotella nigrescens* ; Prevotella* ; Sequence Analysis, Protein

One of the central components of periodontal therapy is the improvement of esthetics. The presence and appearance of interdental papillae plays an important role of periodontal esthetics. The aim of the present study was to investigate how immediate provisional restoration preserve the shape of interdental papilla around the extraction socket and the width of bucco-lingual of gingiva. Another aim was to investigate the change in the interdental papilla and the amount of vertical bone fill of a extraction socket in relation to the interdental alveolar bone levels adjacent the alveolar socket. A total of 19 patients (11 male, 8 female, mean age of 50.57+/-8.16), who visited the Department of Periodontology, Pusan National University and had more than one anterior tooth scheduled to be extracted due to an advanced periodontal disease were included in the present study. After initial periodontal therapy, the extracted teeth were reshaped of the root and placed into the socket followed by splinting with adjacent teeth with self-curing resin. The width of bucco-lingual of gingiva and interdental papilla height were measured at baseline, 1, 3, 6, 9 and 12 month and the periapical radiographic examination were taken at baseline, 6 and 12month following the extraction. The amount of vertical bone fill in the extraction socket were calculated. At 12 months following the extraction, the changes in mesial and distal interdental papilla and the width of bucco-lingual showed -1.06+/-0.48mm, -0.84+/-0.50mm, -1.50+/-0.96mm, relatively. The positional change in the interproximal papillae was significantly associated with the interdental bone level adjacent to the extraction socket(p=0.028). The higher the interproximal bone level adjacent the extraction socket, the greater the amount of bone fill in the extraction socket(p<0.001). In conclusion, it was thought that immediate provisional restoration could minimize the loss of the width of bucco-lingual and interproximal papillae around the extraction socket. In addition, the higher the interproximal bone level adjacent the extraction socket, the greater the amount of bone fill and the smaller the reduction of papillary height around the extraction socket.
Busan ; Esthetics ; Female ; Gingiva ; Humans ; Male ; Periodontal Diseases ; Splints ; Tooth

To evaluate the effects of Dexamethasone(Dex), Platelet derived growth factor-BB(PDGF) and combination of Dex and PDGF(DP) on the growth and differentiation of MC3T3-E1 cells, Dex(10(-7) M) and PDGF(10 ng/ml) in experimental group were added to the cells at the days 5, 10, 15, 20, 25 and examined for cell proliferation activities, DNA synthesis activities, ALP activities and bone nodule formation. The results were as follows : 1.In Dex group, cell proliferation, DNA synthesis and ALP activities were lower until 15 days when compared to the control group. Bone nodules formation were shown at 10 days. 2.In PDGF group, cell proliferation and DNA synthesis activities were higher until 15 days and ALP activities were lower when compared to the control and Dex groups. Bone nodules formation were shown at 20 days. 3.In DP group, cell proliferation and DNA synthesis activities of PDGF were suppressed by Dex and synergistic effects of combination of Dex and PDGF on ALP activities were shown at days 5 when compared to control and Dex groups. Bone nodules formation activities of Dex were suppressed by PDGF.
Blood Platelets ; Cell Proliferation ; Dexamethasone* ; DNA