1.Antimicrobial activity of certain fresh water microalgae from Thamirabarani River, Tamil Nadu, South India
Prakash Wilfred Justella ; MarimuthuAntonisamy Johnson ; Jeeva Solomon
Asian Pacific Journal of Tropical Biomedicine 2011;(z2):170-173
Objective:To evaluate the antimicrobial potential of fresh water microalgae viz., Oscillatoria sancta (O. sancta) (Kuetz) Gomont., Lyngbya birgei (L. birgei) Smith G.M., Oedogonium echinospermum (O. echinospermum), Spirogyra decimina (S. decimina) (Muller) Kuetz., Spirogyra grantiana (S. grantiana) Transeau., Spirogyra crassa (S. crassa), Spirogyra biformis (S. biformis) Jao. and Spirogyra condensata (Vaucher) Kuetz against human bacterial pathogens. Methods:Antimicrobial study was carried out by disc diffusion method against the pathogens viz., Escherichia coli (E. coli) (ATCC 35218), Staphylococcus aureus (S. aureus) (ATCC 6538), Salmonella typhi (S. typhi) (MTCC 733), Proteus vulgaris (P. vulgaris), Proteus mirabilis (P. mirabilis) and Streptococcus pyogenes. Results: The methanolic extract of O. sancta showed the antibacterial activity against three pathogens viz., P. mirabilis, P. vulgaris and S. aureus with the inhibition zones of 10, 8 and 8 mm, respectively. The methanolic and ethanolic extracts of L. birgei exhibited the antibacterial activity against two pathogens i.e. P. mirabilis and P. vulgaris with the maximum zone of inhibition of 8 and 8 mm, respectively. The ethanolic extracts of O. echinospermum displayed the antibacterial activity against S. typhi and P. mirabilis with the maximum of zone of inhibition (7 mm). The methanolic extracts of S. decimina exhibited the antibacterial activity against S. aureus and P. mirabilis with the maximum zone of inhibition of 12 and 9 mm, respectively. The ethanolic extracts of S. grantiana showed the anti-bacterial activity against three organisms i.e. E. coli, P. vulgaris and P. mirabilis with the zone of inhibition of 9, 10 and 9 mm, respectively. The methanolic extracts of S. crassa exhibited the antibacterial activity against P. mirabilis with the maximum zone of inhibition (9 mm). The methanolic extracts of S. biformis exhibited the antibacterial activity against P. vulgaris with the maximum zone of inhibition (8 mm). Conclusions: These results give an indication of the presence of promising antibacterial compounds in the plants under studied. Further phytochemical studies are needed to elucidate the components responsible for antibacterial activity of these extracts against bacteria.
2.Preliminary phytochemical screening of the pericarp of Crataeva magna (Lour.) DC. - a medicinal tree
Kiruba Solomon ; Mahesh Mony ; Paul Miller Zachariah ; Jeeva Solomon
Asian Pacific Journal of Tropical Biomedicine 2011;(z1):129-130
Objective: To examine the phytochemicals present in the pericarp of Crataeva magna (C. magna) (Lour.) DC. which is used as a traditional medicine by the inhabitants of Kanyakumari district. Methods: Phytochemical screening of the pericarp was done to determine the secondary metabolites in various solvents studied. Results: The phytochemical screening on the pericarp of C. magna (Lour.) DC. proved the presence of phytochemicals such as phenols, saponins and tannins. Conclusions: The findings of the present study recommended that the pericarp of C. magna (Lour.) DC. have potential antimicrobial compounds that may be of use for developing plant based drugs for various ailments.
3.Preliminary phytochemical studies of the leaf extracts of Rhododendron arboreum Sm. ssp. nilagiricum (Zenker) Tagg
Kiruba Solomon ; Mahesh Mony ; Paul Miller Zachariah ; Nisha Rajalekshmiamma Sreekandannair ; Jeeva Solomon
Asian Pacific Journal of Tropical Biomedicine 2012;(z3):1338-1340
Objective: To examine the secondary metabolites present in the leaf extracts of Rhododendron arboreum Sm. ssp. nilagiricum (Zenker) Tagg. Methods: Phytochemical screening of the leaf extract was done to determine the phytochemical constituents in the various solvents studied.Results:nilagiricum (Zenker) Tagg. confirm the existence of secondary metabolites such as phenols, saponins and tannins. Conclusions: The study suggests that the leaf extracts of R. arboreum Sm. ssp. nilagiricum (Zenker) Tagg. can be best utilized in developing bioactive compounds against pathogenic infection. The phytochemical study carried out on the leaf extracts of R. arboreum Sm. ssp.
4.Myrica esculenta Buch.-Ham. ex D. Don.-a potential ethnomedicinal species in a subtropical forest of Meghalaya, northeast India
Jeeva Solomon ; Lyndem Grace Febreena ; Sawian Therese Jasmine ; Laloo Christopher Roytre ; Mishra Prakash Banu
Asian Pacific Journal of Tropical Biomedicine 2011;(z2):174-177
Objective: To determine the effect of anthropogenic activities on the population structure and regeneration efficacy of Myrica esculenta (M. esculenta), an ethnomedicinally important tree species in sub-tropical forests of Meghalaya. Methods: The population structure and regeneration potential of M. esculenta were studied by using quadrat method. Results: The regeneration status of M. esculenta was highly influenced by disturbance. Presence of limited number of seedlings in the forest floor denotes the lack of seeds in the forests. The low conversion of seedling into saplings was mainly due to the removal saplings by the indigenous community, who residing in the vicinity of the forest for meeting their requirement of fuel wood. Conclusions:The findings of the present study indicate that overexploitation of M. esculenta may cause threat to extinction from wild. There is an obvious need to explore non timber forest products to a desired pace that will minimize the pressure on a particular species by providing alternative to a species, leading to sustainable utilization of genetic resources.
5.Phytochemical studies on Azolla pinnata R. Br., Marsilea minuta L. and Salvinia molesta Mitch
Mithraja Jalajakumari Muraleedharannair ; Antonisamy Marimuthu Johnson ; Mahesh Mony ; Paul Miller Zachariah ; Jeeva Solomon
Asian Pacific Journal of Tropical Biomedicine 2011;(z1):26-29
Objective: To evaluate the phytochemical properties of Azolla pinnata R. Br., Marsilea minuta L. and Salvinia molesta Mitch. Methods: The dried and powered leaves materials (50 g) were extracted successively with 250 mL of petroleum ether, ethyl acetate, methanol, chloroform, acetone, benzene and water by using Soxhlet extractor for 8 h at a temperature not exceeding the boiling point of the solvent. Phytochemical screening of the extracts was carried out according to the standard methods. Results: Out of eighteen tested extracts, eighteen extracts showed the presence of phenolics. Next to that, fourteen extracts were illustrated their existence of tannin. Ten extracts showed the occurrence of carbohydrates in the crude extracts of the selected plants. Steroid and saponin are present in eight extracts, next to that xanthoprotein is present in six extracts, followed by flavonoid and protein which are present in five extracts. Carboxylic acid showed its presence only in two extracts. Conclusions: From the results, it can be concluded that the three plants extracts show the presence of many bioactive compounds after extensive investigation. We recommend further research on these plants to quantify the concentration of these compounds. Further work will accentuate the isolation and characterization of active principles responsible for bio-efficacy and bioactivity.