1.Embryonic Stem Cell.
Berm Seok OH ; Dong Ho CHOI ; Seung Hyun JEE
Journal of Korean Society of Endocrinology 2001;16(6):584-595
No abstract available.
Embryonic Stem Cells*
2.Clinical study on necrotizing lymphadenitis.
Jee Yeon JANG ; Hyun Rim CHOI ; Jin Woo KIM
Journal of the Korean Academy of Family Medicine 1992;13(6):491-502
No abstract available.
Lymphadenitis*
3.Three Cases of Hemolytic Diseases of Newborns due to Anti - E Antibody.
Hee Joo HONG ; Jee Hyun KIM ; Sun Hee CHOI ; Chong Woo BAE ; Yong Mook CHOI
Korean Journal of Perinatology 2001;12(2):163-167
No abstract available.
Humans
;
Infant, Newborn*
4.Immunohistochemical study on sweat gland tumors with monoclonal antibodies against S-100 subunits.
Kyung Jeh SUNG ; Kwang Hyun CHO ; Ho Seok SUH ; Jee Ho CHOI ; Jai Kyoung KOH
Korean Journal of Dermatology 1992;30(4):446-453
S-100 protein is a mixture of three proteins, that is, S-100 ao(aa), S-100 a(ab) and, S- 100 b(bb). Twenty-two case, of sweat gland tumors were stained with immunoperoxidase technique (ABC method) for the presence of S-100a and b-subunit. Four syringomas, four eccrine poromas, two eccrine porocarcinomas, two ecerine spirdeiomas, one papillary eccrine adenoma, three clear cell hidradenomas, three mixed tumr rs of the skin, two papillary syringocystadenomas, and one cylindroma were included. All specimens were formalin-fixed and paraffin-embedded. The results were as follows : 1) The staining patterns of anti-S-100a and b-protein antibodies we e simillar to those of anti-S-100 protein antibody except in eccrine poroma and porocare nomal. 2) In eccrine poroma and porocarcinoma, scattered S-100-positive dendritic cells within tumor cell nests were stained by S-100-protein antibody (3/6), but not by anti-S-100a protein antibody. S-100p is present in normal Langerhans cells. Therefore this finding suggests that these cells niay be Langerhans cells
Acrospiroma
;
Adenoma
;
Antibodies
;
Antibodies, Monoclonal*
;
Carcinoma, Adenoid Cystic
;
Dendritic Cells
;
Eccrine Porocarcinoma
;
Immunoenzyme Techniques
;
Langerhans Cells
;
Poroma
;
S100 Proteins
;
Skin
;
Sweat Glands*
;
Sweat*
;
Syringoma
5.Immunohistochemical study on granulomatous skin deseases.
Jee Ho CHOI ; Kwang Hyun CHO ; Byung Jick RYU ; Kyoung Jeh SUNG ; Jai Kyoung KOH
Korean Journal of Dermatology 1993;31(5):702-712
BACKGROUND: A definition of granuloma is a focal chronic inflammatory response to tissue injury evolved by a poorly soluble substwice characterized by the accumulation and proliferation of the mono-nuclear histiocytic cells. The accuracy with which rnononuclear cells may be identified in skir. is much improved by the use of both heteroantisera and monoclonal antibodies directed against selected cellular antigens, OBJECTIVE: Our purpose was to examine the staining patterns of anti-lysozyme, anti-a-1-antitrypsin, anti-S-100 protein antibodies, and MAC-387 monoclonal anibody in granulomatous skin diseases. METHOD: We performed imminoperoxidase staining(the labelled str prvidin-biotin peroxidase complex method on the formalin-fixed, paraffin-embedded tissue specimens of granulomatous skin diseases. RESULTS: S-100 protein positive dendritic cells were demonstrated in the granulomatous infiltrates as scattered pattern and MAC-387 positive cells were predominantly found in the center of granulomas, The staining pattern and percentage of positively stained cells of a--antitrypsin were similar to those of lysozyme. A1Pha-1-antitrypsin and lysozyme positive cells w re present in the center as well as lymphohistiocytic infiltrates of granulomas. CONCLUSION: These data sugget that histiocytes are composed of heter igeneous groups of cells such as the mononuclear-phagocyte system and dendritic cell system.
Antibodies
;
Antibodies, Monoclonal
;
Dendritic Cells
;
Granuloma
;
Histiocytes
;
Muramidase
;
Peroxidase
;
S100 Proteins
;
Skin Diseases
;
Skin*
6.Distribution of T lymphocyte Subpopulations in Psoriatic Skin.
Kyung Jeh SUNG ; Hyun Soo KIM ; Jung Wha SUH ; Jee Ho CHOI
Korean Journal of Dermatology 1997;35(3):435-442
BACKGROUND: Although the main features of psoriasis consist of abnormal epidermal proliferation and T cell infiltration, which of these is the initial abnormality has been a longstanding unresolved question. Recently there has been definite evidence that activated T cells produce the cytokines that induce keratinocyte abnormalities. OBJECTIVE: We investigated the distributions and relative numbers of T lymphocyte subpopulations, that is, CD4+ and CD8+ T cells, to verify the more important T cell subtype and its infiltrating site in the formation of psoriatic lesions. METHODS: Paired psoriatic lesional and non-lesional skin tissues were obtained from eight typical psoriatic patients. Immunohistochemical staining was done on the frozen sections using a labelled streptavidin-biotin peroxidase complex method with DAKO LSAB kit. The primary antibodies used in this study were monoclonal or polyclonal antibodies against CD3, CD4, CD8, HLA-DR, and ICAM-1. RESULTS: In lesional psoriatic skin, the epidermis was mainly infiltrated by CD8+ T cells. There were little or no T cells in non-lesional epidermis. In both lesional and non-lesional dermis, CD4+ T cells were the main ones. In lesional skin, anti-ICAM-1 antibody positively stained diffusely in the endothelial cells of papillary and subpapillary plexuses and focally in epidermal keratinocytes, but in non-lesional skin only the endothelial cells in the subpapillary plexus were stained. There were many HLA-DR+T lymphocytes in lesional and non-lesional dermis. In the epidermis, HLA DR was detected only in lesional keratinocytes and T lymphocytes. CONCLUSION: These results suggest (1) participation of activated epidermal CD8+ T lymphocytes in the formation of psoriatic plaques, and (2) a pathogenetic role of ICAM-1 expression on papillary microvessels.
Antibodies
;
Cytokines
;
Dermis
;
Endothelial Cells
;
Epidermis
;
Frozen Sections
;
HLA-DR Antigens
;
Humans
;
Intercellular Adhesion Molecule-1
;
Keratinocytes
;
Lymphocyte Subsets*
;
Lymphocytes*
;
Microvessels
;
Peroxidase
;
Psoriasis
;
Skin*
;
T-Lymphocytes
7.A Novel Visualization Method for Sleep Spindles Based on Source Localization of High Density EEG.
Soohyun LEE ; Seunghwan KIM ; Jee Hyun CHOI
Experimental Neurobiology 2017;26(6):362-368
Equivalent dipole source localization is a well-established approach to localizing the electrical activity in electroencephalogram (EEG). So far, source localization has been used primarily in localizing the epileptic source in human epileptic patients. Currently, source localization techniques have been applied to account for localizing epileptic source among the epileptic patients. Here, we present the first application of source localization in the field of sleep spindle in mouse brain. The spatial distribution of cortical potential was obtained by high density EEG and then the anterior and posterior sleep spindles were classified based on the K-mean clustering algorithm. To solve the forward problem, a realistic geometry brain model was produced based on boundary element method (BEM) using mouse MRI. Then, we applied four different source estimation algorithms (minimum norm, eLORETA, sLORETA, and LORETA) to estimate the spatial location of equivalent dipole source of sleep spindles. The estimated sources of anterior and posterior spindles were plotted in a cine-mode that revealed different topographic patterns of spindle propagation. The characterization of sleep spindles may be better be distinguished by our novel visualization method.
Animals
;
Brain
;
Electroencephalography*
;
Humans
;
Insulator Elements
;
Magnetic Resonance Imaging
;
Methods*
;
Mice
8.Displacement of a lower third molar into the lateral pharyngeal space.
You Sung CHOI ; Yu Jin JEE ; Hyun Chul SONG
Journal of the Korean Association of Oral and Maxillofacial Surgeons 2004;30(6):551-553
The displacement of an entire tooth into the adjacent anatomical area is an uncommon complication of a tooth extraction. We encountered a 23-year-old woman who had previously undergone surgery under local anesthesia to remove the lower third molar about 12 weeks prior and the upper third molar was extracted 2 days prior to visiting this hospital. Upon admission, she complained of a swallowing discomfort and a mouth opening limitation. Panoramic radiograph and a CT scan revealed a displacement of the entire tooth into the lateral pharyngeal space. The tooth was retrieved via the transoral approach under general anesthesia. The removed tooth had an indentation formed by a dental bur. Therefore, it was concluded that the tooth displaced into the lateral pharyngeal space was the lower third molar. This report describes an unusual case of a third molar that was displaced into the lateral pharyngeal space with a review of the relevant literature.
Anesthesia, General
;
Anesthesia, Local
;
Deglutition
;
Female
;
Humans
;
Molar, Third*
;
Mouth
;
Tomography, X-Ray Computed
;
Tooth
;
Tooth Extraction
;
Young Adult
9.The Relationship of VO2Max/Min in cardiopulmonary exercise test and fat distribution.
Jae Chol CHOI ; Hyun Suk JEE ; Young Bum PARK ; Sung Jin PARK ; Jee Hoon YOO ; Jae Yeol KIM ; In Won PARK ; Byoung Whui CHOI ; Sung Ho HUE
Tuberculosis and Respiratory Diseases 2000;49(4):495-501
BACKGROUND: Cardiopulmonary exercise test is a useful test for the evaluation of the cardiovascular and respiratory systems. Obese subjects have an increased resting metabolic rate (VO2) compared to non-obese subjects and the increase is more marked during dynamic exercise, which results in the limitation of maximal exercise in obese subjects. In this study, the influence of the obesity and fat distribution on the maximal exercise capacity were evaluated. METHODS: Maximal exercise capacity was represented by maximam maximum oxygen uptake and VO2 max in the cardiopulmonary test. Obesity, total fat content and abdominal obesity(waist to hip ratio, WHR) were measured by bioelectrical impedence method. Total of 42 volunteers(male 22, female 20) were evaluated. RESULTS: 1) Weight to height ratio(mean±SD) was 110%±14.9% in men and 100±11.1% in women. 2) Fat ratio(mean±SD) was 23.3±5.2% in men and 27.55±3.9% in woman. 3) Waist to hip ratio(mean±SD) was 0.85±0.04 in men and 0.8±0.03 in woman. 4) In men, VO2max/min/Kg was negatively correlate with obesity, fat ratio, and abdominal fat distribution. 5) In woman, VO2max/Kg was negatively correlated with obesity and fat ratio, but did not show significant relationship with abdominal fat distribution. CONCLUSION: Obesity was a limiting factor for maximal exercise in both men and women. Abdominal obesity was a limiting factor for maximal exercise in men but its implication to women needs further evaluation.
Abdominal Fat
;
Exercise Test*
;
Female
;
Hip
;
Humans
;
Male
;
Obesity
;
Obesity, Abdominal
;
Oxygen
;
Respiratory System
10.A Case of Primary Lateral Sclerosis Showing Degeneration ofCorticospinal Tract on Magnetic Resonance Imaging.
Jee Young OH ; Hyun Jung YU ; Jee Hyun KIM ; Jung Eun KIM ; Kyoung Gyu CHOI ; Kee Duk PARK
Journal of the Korean Neurological Association 2000;18(6):801-804
Primary lateral sclerosis (PLS) is a rare upper motor neuron disease characterized by selective degeneration of corticospinal and corticobulbar tracts. It is important to rule out other diseases presenting as progressive spastic paraparesis, such as multiple sclerosis (MS) or amyotrophic lateral sclerosis (ALS). Though that is not so difficult as the development of imaging technique and neurophysiological tool, if one shows slow progressive spastic paraparesis clinically, and there is no abnormal CSF or EMG findings, it is not easy to differentiate between primary progressive MS and PLS. In fact, PLS is so rare that it has long been debated whether PLS is a disease entity or syndrome or a nothing. But we present a PLS patient whose brain MRI shows diffuse signal change along the bilateral corticospinal tracts and the hypoperfusion of frontal motor cortex is proved by brain SPECT.
Amyotrophic Lateral Sclerosis
;
Brain
;
Humans
;
Magnetic Resonance Imaging*
;
Motor Cortex
;
Motor Neuron Disease*
;
Multiple Sclerosis
;
Paraparesis, Spastic
;
Pyramidal Tracts
;
Tomography, Emission-Computed, Single-Photon