1.Serotype- and serogroup-specific detection of African horsesickness virus using phage displayed chicken scFvs for indirect double antibody sandwich ELISAs.
Wouter VAN WYNGAARDT ; Cordelia MASHAU ; Isabel WRIGHT ; Jeanni FEHRSEN
Journal of Veterinary Science 2013;14(1):95-98
There is an ongoing need for standardized, easily renewable immunoreagents for detecting African horsesickness virus (AHSV). Two phage displayed single-chain variable fragment (scFv) antibodies, selected from a semi-synthetic chicken antibody library, were used to develop double antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISAs) to detect AHSV. In the DAS-ELISAs, the scFv previously selected with directly immobilized AHSV-3 functioned as a serotype-specific reagent that recognized only AHSV-3. In contrast, the one selected with AHSV-8 captured by IgG against AHSV-3 recognized all nine AHSV serotypes but not the Bryanston strain of equine encephalosis virus. Serving as evidence for its serogroup-specificity. These two scFvs can help to rapidly confirm the presence of AHSV while additional serotype-specific scFvs may simplify AHSV serotyping.
African horse sickness virus/*isolation & purification
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Animals
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Antibodies, Immobilized
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Antibodies, Viral/*immunology
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Cercopithecus aethiops
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Chickens
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Enzyme-Linked Immunosorbent Assay/methods/*veterinary
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Immunoglobulin G
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*Peptide Library
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Serologic Tests/methods/veterinary
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Serotyping
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Single-Chain Antibodies/*immunology
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Vero Cells