1.Immunohistopathologic Changes in Experimental Allergic Encephalomyelitis.
Woo Ho KIM ; Je G CHI ; Sang Kook LEE
Korean Journal of Pathology 1986;20(3):277-287
Experimental allergic encephalomyelitis (EAE) has been a well established animal model of postvaccinatal demyelinating diseases occurring in humans. Therefore elucidation of its pathogenesis would be very critical for the understanding of various human demyelinating diseases including multiple sclerosis. This study was performed to characterize the infiltrating cells in inflammatory sites and analyze the nature of the damage of blood brain barrier in experimental allergic encephalomyelitis. Experimental allergic encephalomyelitis was produced by administering homologous spinal cord homogenate together with complete Freund's adjuvant in guinea pigs. Immunostainings on guinea pig IgG, IgM, IgA and muramidase were performed by peroxidase-antiperoxidase or indirect immunofluorescent methods. The blood-brain barrier change was assessed by administering fluorescent Evans blue. Following results were made. In juvenile animals, both clinical findings and histopathologic changes were first noted by 3 weeks after injection and progressed during the whole experimental period. However, these findings were delayed in onset and low in incidence in adult animals. The clinical and pathologic changes started from the caudal portions and extended rostrally. The blood-brain barrier (BBB) was damaged and progressed starting also from the caudal portion of the spinal cord. The BBB changes were more severe in young animal than adult animals. Those changes preceded th histologic alterations. It is suggested that the BBB susceptibility is responsible for the caudal onset of histologic changes. Although the lesion has been thought to be induced by T-cell mediated hypersensitivity, infiltrating cells consisted mainly of muramidase positive histiocytes. A few immunoglobulin positive B cells or plasma cells could also be demonstrated in the lesion. The former usually infiltrated the parenchyme and the latter remained around the small or medium-sized vessels.
Adult
;
Male
;
Female
;
Humans
;
Animals
;
Incidence
2.Retropsoas peritoneal recess in CT.
Je Ho WOO ; Jong Oag PARK ; Jong Hwa LEE ; Jin Woo CHUNG ; Don Young LEE
Journal of the Korean Radiological Society 1993;29(2):300-303
Retropsoas space below the level of kidney has been suggested as a portion of inferior extensions of perirenal and anterior and posterior pararenal spaces. With this being true, the space may play an important role in disease extension. A study was performed to verify the existence of retropsoas peritoneal recess by means of identifying the extension of bowel loops into this space. Abdominal CT scans of 146 cases evaluated retrospectively revealed extension of 5 small bowel and 7 large bowel loops (6 descending and 1 ascending colons)(n=12/8.2%) into the retropsoas space verifying its existence. Since pathologic collection within the retropsoas space might be falsely inter preted as a retroperitoneal pathology and percutaneous uroradiologic intervention could result in intraperitoneal injury or contamination without the knowledge on the existence of this space, observation of this space is essential in CT scans.
Kidney
;
Pathology
;
Retrospective Studies
;
Tomography, X-Ray Computed
3.Plastination: An Improved Method for Preservation of Pathology Specimens.
Chong Woo YOO ; Min Ho CHOO ; Sa Sun CHO ; Sang Kook LEE ; Je Geun CHI ; Woo Ho KIM
Korean Journal of Pathology 1998;32(7):531-534
The gross tissue specimens are a valuable aid to the teaching of pathology and anatomy. However, traditional methods for storage and handling of them are discouragingly difficult and, recently, minimal surgical resections as well as preoperative interventions make it more difficult to have instructive gross specimens. Plastination is a process of tissue preservation by impregnation with silicone polymers or epoxy resins. The process in our study involves dehydration by cryosubstitution in aceton, defatting, forced impregnation of silicon polymer in a vacuum, curing and finishing. We submitted 40 surgically resected specimens to plastination. The resulting specimens are odorless, relatively dry, durable, life-like, non-hazardous, maintenance-free, and do not deteriorate with time. Plastinated specimens are a useful adjunct to the teaching of pathology, particularly suited for use in small groups, and appropriate method of tissue preservation. They are much preferred to wet preparation and conventional pots by both students and teachers owing to their accessibility, superior illustrative powers, and comparative ease of interpretation.
Dehydration
;
Epoxy Resins
;
Humans
;
Pathology*
;
Polymers
;
Silicones
;
Tissue Preservation
;
Vacuum
4.Purification of the urease of helicobacter pylori and production of monoclonal antibody to the urease of helicobacter pylori.
Jae Im KIM ; Seung Chul BAIK ; Myung Je CHO ; Woo Kon LEE ; Kwang Ho RHEE
Journal of the Korean Society for Microbiology 1991;26(6):531-540
No abstract available.
Helicobacter pylori*
;
Helicobacter*
;
Urease*
5.Pressor Effect of Intracerebroventricular Diphenhydramine and Ranitidine in Rabbits.
Han Ho CHO ; Soo Han KIM ; Sam Suk KANG ; Je Hyuk LEE ; Jung Hyun WOO
Journal of Korean Neurosurgical Society 1991;20(10-11):905-910
This study was undertaken to observe the effects of centrally administred antihistamines on the blood pressure. Diphenhydramine(DPH), a H1-receptor antagonist, and ranitidine(RAN), a H2-receptor antagonist were administered intracerebroventricularly(icv) on urethane-anesthetized rabbits. 1) Both DPH and RAN administered intraccebroventricularly increased blood pressure, however the intravenous(iv) adminstration of them did not affect blood pressure. The pressor response to icv DPH was dose-dependent, but that to icv RAN was not. 2) The pressor response to icv DPH(1mg) was either markedly attenuated or reversed to depressor response by the pretreatment with icv phentolamine(250,500ug), and iv chlorisondamine(0.1, 1mg/Kg) and iv phenoxybenzamine(1mg/Kg). In cord-sectioned rabbtis, icv RAN) 1mg) did not produce pressor response. 3) The pressor responsr to icv RAN(1mg) was not affected by the pretreatment with icv phentolamine(500ug), iv chlorisondamin(1mg/Kg) and iv phenoxybenzamine(1mg/Kg), and iv phenoxybenzamine(1mg/Kg). RAN also producted pressor response in cordsectioned rabbits. These results suggest that the pressor response to icv DPH is elecited by increasing peripheral sympathetic tone via the stimulation of central alpha-adrenoreceptors and the pressor response to icv RAN is produced by releasing some humoral facotr which can increase blood pressure.
Blood Pressure
;
Diphenhydramine*
;
Histamine Antagonists
;
Rabbits*
;
Ranitidine*
6.Development of diagnostic method of helicobacter pylori infection: I. molecular cloning and DNA sequencing of urease.
Cheol Keun PARK ; Woo Kon LEE ; Young Mi DOH ; Myung Je CHO ; Kwang Ho RHEE
Journal of the Korean Society for Microbiology 1991;26(6):541-552
No abstract available.
Cloning, Molecular*
;
DNA*
;
Helicobacter pylori*
;
Helicobacter*
;
Sequence Analysis, DNA*
;
Urease*
7.Production of the monoclonal antibody and the genomic library of helicobacter pylori.
Kwang Ho RHEE ; Woo Kon LEE ; Seung Chul BAIK ; Myung Je CHO ; Hyu Jin CHOI
Journal of the Korean Society for Microbiology 1991;26(4):305-316
No abstract available.
Genomic Library*
;
Helicobacter pylori*
;
Helicobacter*
8.A Case of Advanced Gastric Cancer, Developed from Endoscopic Diagnosis of Early Gastric Cancer.
Je Young WOO ; Ki Ho SONG ; In Sik CHUNG ; Hee Sik SUN ; Boo Sung KIM
Korean Journal of Gastrointestinal Endoscopy 1988;8(1):29-32
Early gastric cancer is defined carcinoma that is limited to the gastric mucosa and submucosa and that does not extend into the muscularis propria. The development and widespread use of upper gnstrointestinal endoscopy with biopsy and cytology have enhanced our ability to make early diagnosis of gastrie carcinoma. Gastric cancinoma is being diagnosed at an ealry stage aad the survival rates for these patients are significantly greater than those with advanced gastric carcinoma. These ealry lesion can be cured in over 90% of the cases. In fact, it is almost impossible to follow up cases of early gastric cancer without surgical treatment. We report a case developed advanced gastric carcinoma 34 months after initial endoseopic diagnosis of early gastric cancer. A, 48-year-old woman was diagnosed early gaatrie cancer on June 21, 1985 by endoscopy. Proper surgical intetvention was not carried out due to her liver cirrhosis. The follow-up endoscopic examination was done on March 16, 1988. The gastric cancer looked like an advanced stomach and extended to other site.
Biopsy
;
Diagnosis*
;
Early Diagnosis
;
Endoscopy
;
Female
;
Follow-Up Studies
;
Gastric Mucosa
;
Humans
;
Liver Cirrhosis
;
Middle Aged
;
Stomach
;
Stomach Neoplasms*
;
Survival Rate
9.Ultrastrual Study on Changes of the Capillaries in Experimental Brain Infarcts of Rats.
Jin Ho CHO ; Je Hyuk LEE ; Sang Woo JUHNG
Journal of Korean Neurosurgical Society 1987;16(1):203-216
Experimental brain infarcts in rats were studied light and electron microscopically to investigate the factor(s) controlling contrast enhancement on CT scans of the infarcts. Brain infarction was induced by injection of fine autologous blood clots through the right internal carotid artery and the affected brain was processed for examination 1 day, 2 day, and 1, 2, 4, 6 and 8 weeks after the injection. The lesion of early, necro - degenerative stage(1 day to 1 week) showed structural disintegration of capillary endothelial cells with dissolution of tight junctions. The lesion of middle, regenerative stage(2 to 4 weeks) was characterized by proliferation of new capillaries, They had well - formed tight junctions and continuos basement membrane. The endothelial cells, however, had intraluminal villous projections and many pinocytotic vesicles, suggesting an increased permeability. The capillaries matured at late, reparative stage(8 weeks), appearing similar to those in normal brain tissue except that they were loosely surrounded by astrocytic foot processes. It was presumed that the ncreased permeability of new capillaries might play an important role for contrast enhancement observable clinically during the middle stage(2 to 4 weeks).
Animals
;
Basement Membrane
;
Brain Infarction
;
Brain*
;
Capillaries*
;
Carotid Artery, Internal
;
Endothelial Cells
;
Foot
;
Permeability
;
Rats*
;
Tight Junctions
;
Tomography, X-Ray Computed
10.Chest radiographic findings of scrub typhus: An analysis of 160 cases occurred in Ulsan area.
Ok Hwa KIM ; Dong Heon OH ; Ki Sung KIM ; Je Ho WOO ; Jung Hyeok KWON
Journal of the Korean Radiological Society 1993;29(2):205-210
Scrub typhus (Tsutsugamushi disease)is an acute febrile systemic illness caused by Rickettsia tsutsugamushi that is transmitted to humans by the bite of larval-stage trombiculid mites (chiggers). The authors analyzed chest radiographic findings of scrub typhus in 160 patients in Ulsan area. One hundred and eight (67.5%) of160 patients showed abnormal findings which included lung lesions in 108 patients (67.5%), cardiomegaly in 37 patients (23.1%), lymphadenopathy in 25 patients (15.6%) and pleural effusion in 11 patients (6.9%). Among the lung lesions, interstitial patterns were seen in 107 patients (66.9%), mostly fine or medium reticulonodular, and air-space patterns in 14 patients(8.8%) and combined interstitial and air-space patterns in 13 patients (8.1%). Sixty-four patients(40%) had combined chest radiographic findings. The typical chest radiographic findings of scrub typhus would be helpful in evaluation of the causes of acute febrile illness that occur during late fall in the endemic area.
Cardiomegaly
;
Humans
;
Lung
;
Lymphatic Diseases
;
Orientia tsutsugamushi
;
Pleural Effusion
;
Radiography, Thoracic*
;
Scrub Typhus*
;
Thorax*
;
Trombiculidae
;
Ulsan*