PURPOSE: To determine the role of Insulin-like Growth Factor-I (IGF-I) in the regulation of Vascular Endothelial Growth Factor (VEGF) expression in MG-63 cells and then to find the mechanism b which this regulation occurs. MATERIALS AND METHODS: MG-63 cells were grown to confluence in 60-mm dishes. To determine the effects of IGF-I on expression of VEGF mRNA according to time and concentration, the cells were treated with 10 nM IGF-I, following isolation of total RNA and Northern blot analysis after 1, 2, 4, 8, 12, 24 hours and after 2 hours of treatment with 0.5, 2, 10, 25, 50 nM IGF-I respectively, isolation of total RNA and Northern blot analysis were followed. To determine the mechanism of action of IGF-I, inhibitors such as hydroxyurea (76.1 microgram/ml), actinomycin D (2.5 microgra/ml), cycloheximide (10 microgram/ml) were added 1 hour after treatment of 10 nM IGF-I. RESULTS: 1. the expression of VEGF mRNA was increased with treatment of IGF-I. 2. The expression of VEGF mRNA was increased according to time- and concentration dependent manner of IGF-I. 3. The effect of IGF-I was decreased by hydroxyuera, actinomycin D, but not by cycloheximide. CONCLUSION: IGF-I regulate the expression of VEGF mRNA in the level of DNA synthesis and transcription. These results could suggest that IGF-I plays an important role in angiogenesis in the process of new bone formation and remodeling.
Blotting, Northern ; Cycloheximide ; Dactinomycin ; DNA ; Hydroxyurea ; Insulin-Like Growth Factor I* ; Osteogenesis ; RNA ; RNA, Messenger* ; Vascular Endothelial Growth Factor A*

Accidents, Traffic ; Female ; Humans ; Male ; Mandibular Fractures ; Maxillofacial Injuries ; Retrospective Studies ; Seoul

Anatomic Landmarks ; Dental Occlusion ; Dentofacial Deformities ; Diagnosis ; Follow-Up Studies ; Maxillary Osteotomy ; Orbit ; Tomography, X-Ray Computed

The aim of this study was to evaluate implant stability placed in the maxillary sinus which was augmented with bovine bone mineral (Bio-Oss.) mixed with autogenous bone from the maxillary tuberosity. Maxillary sinus floor augmentation with the mixture of bovine bone mineral and autogenous maxillary tuberosity bone was performed in 30 maxillary sinuses, and 68 implants were placed at the time of sinus graft. After 6 months of implant placement abutments were connected and implant stability quotient (ISQ) was measured by radio frequency analysis (RFA). In addition, bone level changes was evaluated by taking periapical radiograph. During surgical procedures, no complication was observed, and all patients healed uneventfully. At 6 months the implant showed stable ISQ values. The marginal bone level changes around the fixtures was stably maintained through out the follow up period. This study confirmed that maxillary sinus floor augmentation with mixture of bovine bone mineral and maxillary tuberosity bone could be reliable for bone regeneration in subantral space.
Bone Regeneration ; Dental Implants ; Follow-Up Studies ; Humans ; Maxillary Sinus* ; Sinus Floor Augmentation ; Transplants

BACKGROUND: The glucose -acidifying genomic species 1, 2, 3 and 13 of the genus Acinetobacter are highly related genetically and may be difficult to differentiate by phenotypic identification schemes using biochemical tests. The aim of this study was to explore the brief restriction enzyme profiles of amplified ribosomal DNA restriction analysis (ARDRA) to identify medically important species of Acinetobacter. Using ARDRA analysis, we evaluated the ID 32 GN system (bioMerieux, Lyon, France) for the identification of A. baumannii (genospecies 2). METHODS: A collection of 78 A. baumannii stains initially identified by the ID 32 GN system was used to determine its accuracy by ARDRA analysis. ARDRA was performed with 10 different restriction enzymes, AluI (AGCT), CfoI (GCGC), HaeIII (GGCC), HinfI (GANTC), MboI (GATC), MspI (CCGG), NciI (CCGG), RsaI (GTAC), ScrFI (CCNGG) and TaqI (TCGA). RESULTS: The combination of restriction patterns obtained with respective enzymes AluI, CfoI and MboI allowed for the discriminatory value for the identification of medically important genospecies such as genospecies 2 (A. baumannii), 3 and 13. By comparing ARDRA results of the 78 strains previously identified by ID 32 GN system, we found the correlation rate between the two systems to be 88.5% (69/78). Nine strains were identified as Acinetobacter genospecies 13 by ARDRA. CONCLUSIONS: This result suggests that the ID 32 GN system may have difficulty in discriminating A. baumannii from genospecies 13. This revised ARDRA method gives a relatively rapid and definitive result for the identification of medically important genospecies of Acinetobacter.
Acinetobacter ; Acinetobacter baumannii* ; Coloring Agents ; DNA, Ribosomal* ; Glucose

Ameloblastoma of the maxilla is an unusual epithelial tumor of odontogenic origin. According to many authors and reports, ameloblastoma account for approximately 1% of all tumors of the jaws, but when pseudo-tumors and cysts are excluded, the ratio rises to 11%. Of these tumors,80% originate in the mandible, while 20% originate in the maxilla. Although it is considered benign histopathologically, it can behave in a slowly growing infiltrative fashion, with multiple recurrences and eventual intracranial, or even distant, spread. We clinically analyzed common site in maxilla, radiographic findings, recurrence rate, duration between treatment and recurrence, the presence and site of distant metastasis in 15 patients who were diagnosed as ameloblastoma of the maxilla and took treatments from 1985 to 1999 in Department of Oral and Maxillofacial Surgery, Dental Hospital, Seoul National University. In this paper, treatment outcomes and our clinical experiences of maxillary ameloblastoma are reported with review of literatures.
Ameloblastoma* ; Humans ; Jaw ; Mandible ; Maxilla* ; Neoplasm Metastasis ; Recurrence ; Seoul ; Surgery, Oral

BACKGROUND: The efficient collection of peripheral blood stem cells (PBSC) from donors who donate for allogeneic transplants as well as from patients undergoing autologous transplants is essential for a successful transplant. Recently, the Amicus cell separator and the associated MNC collection computer software program for PBSC collection were introduced in Korea. METHODS: Two apheresis machines (Amicus, Baxter Healthcare; and CS-3000 plus, Baxter Healthcare) were compared retrospectively. A total number of 144 procedures were performed on 14 donors and 28 patients. The pre- and post-apheresis complete blood cell (CBC) counts and the number of hematopoietic progenitor cells (HPC) were determined in the peripheral blood from the subjects. The CBC, HPC, CD34+ cell counts and the level of colony-forming unit-granulocyte-macrophages (CFU-GM) were measured in the PBSC product collected from both machines. RESULTS: Both machines collected a similar number of CD34+ cells from the donors and patients. On the other hand, the Amicus collected significantly more nucleated cells, MNCs, HPCs and CFU-GM in the patients with significantly less RBC contamination than those with CS-3000 plus. The decrease in the peripheral blood platelet counts in the donors and patients was more prominent after apheresis using the CS-3000 plus (117.00+/-42.75 x 10(3)/microliter, 61.22+/-43.62 x 10(3)/microliter) than Amicus (26.04+/-18.68 x 10(3)/microliter, 22.15+/-28.66 x 10(3)/microliter)(p<0.05). CONCLUSION: PBSC collection can be performed successfully using CS-3000 plus and Amicus. Amicus is superior to CS-3000 plus in avoiding apheresis-induced thrombocytopenia, and is expected to prevent unnecessary platelet transfusion.
Autografts ; Blood Cells ; Blood Component Removal ; Cell Count ; Delivery of Health Care ; Granulocyte-Macrophage Progenitor Cells ; Hand ; Hematopoietic Stem Cells ; Humans ; Korea ; Platelet Count ; Platelet Transfusion ; Retrospective Studies ; Stem Cells* ; Thrombocytopenia ; Tissue Donors

No abstract available.
Gene Ontology* ; Uterine Cervical Neoplasms*

Alveolar Ridge Augmentation ; Animals ; Durapatite ; Factor VIII ; Fibrin Tissue Adhesive ; Fibrin ; Fibrinogen ; Hemostasis ; Humans ; Polymers ; Rats ; Skin ; Subdural Space ; Surgery, Oral ; Sutures ; Thrombin ; Tissue Adhesives ; Tooth Extraction ; Transplants ; Wound Healing

Denture, Overlay ; Esthetics ; Follow-Up Studies ; Humans ; Phonetics ; Survival Rate ; Tooth ; Transplants