1.In vitro platelet assessment of the stored CPDA-1 platelet concentrates.
Kan Hee HAN ; Jang Soo SUN ; Nam Kyung KIM ; Jay Sik KIM ; Dal Hyo SONG
Korean Journal of Blood Transfusion 1992;3(2):143-150
No abstract available.
Blood Platelets*
2.Anti-HBc IgM and anti-delta screening by EIA method.
Journal of Korean Medical Science 1986;1(1):5-13
The clinical value of an enzyme-linked immunosorbent assay (ELISA) for the detection of anti-HBc IgM was evaluated by testing 202 sera from acute viral hepatitis B (AVHB), hepatitis B (HB), chronic hepatitis (CAH), chronic liver disease (CLD), cirrhosis, primary hepatoma, HBsAg carrier, acute viral hepatitis A (AVHA), hepatitis A (HA), non-A, non-B (NANB) hepatitis and miscellaneous conditions other than hepatic disease, and 19 additional various hepatic disease cases were examined for anti-delta. In clinical situations the accurate diagnosis of HB is not always possible and the differential diagnosis seems to be very important especially in making decisions of treatment and estimation of prognosis. In overall cases the highest positive rate of anti-HBc IgM was found in AVHB as shown as 74.3% (26/35) comparing to other conditions in which the positive rate was extremely low (2.1%). The anti-HBc IgM appeared to be highly specific to AVHB (83.9%) as compared to the other. The positive rate of HBsAg was high in AVHB, CAH and HBsAg carrier (100.0%) followed by CLD, cirrhosis and HB (up to 70.8%). The ALT activities and ALPalb fractions were significantly high in AVHB (p less than 0.005). The correlation between the positivity of anti-HBc IgM and highly abnormal ALT appeared be high. AVHB was confined mostly to 10-20 age group and the male to female ratio was about 6 to 1. Subgroup of AVHB II with positive anti-HBc IgM appeared to have a greater chance being positive for HBsAg and ALPalb. The S/N ratio of anti-HBc IgM was as high as 20 which was unique to AVHB.
Adolescent
;
Adult
;
Biological Markers
;
Child
;
Diagnosis, Differential
;
Female
;
Hepatitis/*diagnosis
;
Hepatitis Antibodies/*analysis
;
Hepatitis B/diagnosis/immunology
;
Hepatitis B Antibodies/analysis
;
Hepatitis Delta Virus/*immunology
;
Humans
;
*Immunoenzyme Techniques
;
Immunoglobulin M/immunology
;
Isoenzymes/immunology
;
Male
;
Middle Aged
3.An overview on reality of Dong-In Uywon(Hospital) and effects on culture of medicine.
Korean Journal of Medical History 1999;8(1):45-58
From a review on the reality of Dong-In Hospital which was a hospital founded by Dong-Aa Dong-In-Hywe Foundation which was a corporation of governmental patron around Ulsa(1905) protective treaty between Japan and Korea, and this hospital seemed to be dissoluted just before the annexation signing of Korea to Japan. The building with site of Dong-In Hospital in Taegu was sold to Kyung-Sang Pook-Do province and Jaa-Hye Hospital was constructed instead as a virtual conscience. So it is impossible to say this Jaa-Hye Hospital as the predecessor of Dong-In Hospital although Jaa-Hye Hospital which was belonging to Kyung-Sang Pook-Do province as Kyung-Sang Pook-Do Hospital had changed the name several times until being Taegu Medical School Hospital by using as Taegu Medical Institute College Hospital which was absorbed to U.S. military government after the restoration of independence from Japan. Since 1953 Kyungpook National University absorbed Taegu Medical School, it is possible to use Taegu Medical School Hospital as the predecessor of Kyungpook National University Hospital whereas it is impossible to use Jaa-Hye Hospital as the predecessor with nationality on the basis of the health and medical administrative system.
English Abstract
;
History of Medicine, 20th Cent.
;
Hospitals/*history
;
Korea
;
*Politics
4.Characterization of Heparin:PF4 Isoantibody Interaction to Platelets in Heparin-Induced Thrombocytopenia/ Thrombosis.
Ki Youn KIM ; Yoon Jong CHANG ; Jang Soo SUH ; Jay Sik KIM
Korean Journal of Clinical Pathology 1997;17(6):944-955
BACKGROUND: Heparin-induced thrombocytopenia/ thrombosis (HITT) is recognized as the most frequent and fatal symptom complexes in patients receiving heparin therapy. The antibodies of HITT are not directly bound to heparin but bound to complexes of heparin and platelet factor 4 (PF4) derived from platelet alpha-granules. That is, HITT IgG antibody-heparin-PF4 immune complexes are bound to FcgammaRII receptor of platelets, which induced thrombocytopenia. Some researches showed the antibodies reactive to platelets could be IgM or IgA as well as IgG. So in this study, the authors tried to explain the molecular basis of heparin-PF4-isoantibody complexes . METHODS: In HITT patients who had received long-term heparin therapy, we determined HITT isoantibodies and titers using heparin:PF4 ELISA. When fifteen HITT patients with high titer antibodies (more than 1 : 100) were selected, reaction patterns of isoantibodies with the platelets were examined through serotonin release test and flow cytometry. RESULTS: All patients showed one or more isotype antibodies and the most frequent isotype was IgGl (nine patients) . In the presence of optimal concentra pion of heparin and PF4, ten patients had antibodies which activated platelets, and all of them were positive in serotonin release test. Reactive plasmas had IgGl, IgG3, IgA or IgM antibodies, and each of them except one had IgGl. These platelet activations could be blocked in vitro by anti-IV.3 antibody. Non-reactive plasmas were negative In serotonin release assay nor had TgGl. The plasmas 4hat had two or more isoantibodies showed a similar pattern of the IgG antibody by flow cytometry. CONCLUSIONS: The HITT antibodies can be all kinds of antibody isotopes, but IgA and IgM may not bind to the platelets directly. It seems to be possible only after reacting with heparin-PF4-IgG complexes.
Antibodies
;
Antigen-Antibody Complex
;
Blood Platelets
;
Enzyme-Linked Immunosorbent Assay
;
Flow Cytometry
;
Heparin
;
Humans
;
Immunoglobulin A
;
Immunoglobulin G
;
Immunoglobulin M
;
Isoantibodies
;
Isotopes
;
Mesons
;
Plasma
;
Platelet Activation
;
Platelet Factor 4
;
Serotonin
;
Thrombocytopenia
;
Thrombosis*
5.Diagnostic of D-dimer latex test in head trauma patients progressing to disseminated intravascular coagulation.
Han Gil KIM ; Jang Soo SUH ; Kyung Eun SONG ; Won Kil LEE ; Jay Sik KIM
Korean Journal of Hematology 1991;26(1):59-64
No abstract available.
Craniocerebral Trauma*
;
Disseminated Intravascular Coagulation*
;
Head*
;
Humans
;
Latex*
6.Effect of resuspension patterns as different conditions of centrifusion in use of U bottomed microplate.
Lan Hee HAN ; Jang Soo SUH ; Kyung Eun SONG ; Won Gil LEE ; Jay Sik KIM
Korean Journal of Blood Transfusion 1991;2(1):63-68
No abstract available.
7.Mechanism of aging and prevention.
Immune Network 2001;1(2):104-108
Aging is a senescence and defined as a normal physiologic and structural alterations in almost all organ systems with age. As Leonard Hayflick, one of the first gerontologist s to propose a theory of biologic aging, indicated that a theory of aging or longevity satisfies the changes of above conditions to be universal, progressive, intrinsic and deleterious. Although a number of theories have been proposed, it is now clear that cell aging (cell senescence) is multifactorial . No single mechanism can account for the many varied manifestations of biological aging. Many theories have been proposed in attempt to understand and explain the process of aging. Aging is effected in individual by genetic factors, diet, social conditions, and the occurrence of age-related diseases as diabetes, hypertension, and arthritis. It involves an endogenous molecular program of cellular senescence as well as continuous exposure throughout life to adverse exogenous influences, leading to progressive infringement on the cell's survivability so called wear and tear. So we could say the basic mechanism of aging depends on the irreversible and universal processes at cellular and molecular level. The immediate cause of these changes is probably an interference in the function of cell's macromolecules-DNA, RNA, and cell proteins-and in the flow of information between these macromolecules. The crucial questions, unanswered at present, concerns what causes these changes in truth. Common theories of aging are able to classify as followings for the easy comprehension. 1. Biological, 1) molecular theories-a. error theory, b . programmed aging theory, c. somatic mutation theory, d. transcription theory, e. run-out-of program theory, 2) cellular theories-a. wear and tear theory, b . cross-link theory, c. clinker theory, d. free radical theory, e. waste product theory, 3) system level theory-a. immunologic/autoimmune theory, 4) others-a. telomere theory, b . rate of living theory, c. stress theory, etc. Prevention of aging is theoretically depending on the cause or theory of aging. However no single theory is available and no definite method of delaying the aging process is possible by this moment . The most popular action is anti-oxidant therapy using vitamin E and C, melatonin and DHEA, etc. Another proposal for the reverse of life-span is TCP-17 and IL-16 administration from the mouse bone marrow B cell line study for the immunoglobulin VDJ rearrangement with RAG-1 and RAG-2. Recently conclusional suggestion for the extending of maximum life-span thought to be the calory restriction.
Aging*
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Animals
;
Arthritis
;
Bone Marrow
;
Cell Aging
;
Cell Line
;
Comprehension
;
Dehydroepiandrosterone
;
Diet
;
Hypertension
;
Immunoglobulins
;
Interleukin-16
;
Longevity
;
Melatonin
;
Mice
;
RNA
;
Social Conditions
;
Telomere
;
Vitamin E
;
Vitamins
;
Waste Products
8.CYFRA 21-1, The New Marker for Lung Cancer.
Kyung Ja CHO ; Kyung Hae LEE ; Joong Won LEE ; Kyung Eun SONG ; Won Kil LEE ; Jay Sik KIM ; Jay Yong PARK
Korean Journal of Clinical Pathology 1997;17(1):55-64
BACKGROUND: Cytokeratins were subdivided into 20 different cytokeratins. It is known that cytokeratin 19 is particularly abundant in carcinoma of the lung. METHOD: A sandwich enzyme-linked immunosorbent assay called CYFRA 21-1, combining two monoclonal antibodies directed specifically aganist cytokeratin 19 was done by ES-300(Boehringer Mannheim, Germany). We investigated the diagnostic value of the new tumor marker CYFRA 21-1 in lung cancer in comparison to carcinoembryonic antigen(CEA) and neuron specific enolase(NSE) in the pilot study on 39 serum samples from lung cancer (17 squamous cell cancer, 11 adenocarcinoma, 11 small cell carcinoma) and 51 benign lung diseases and 41 normal healthy persons. RESULTS: Postulating a specificity 95% versus benign lung disease and healthy group, the diagnostic sensitivity of CYFRA 21-1 in lung cnacer independent of histologic type was 43.6% whereas CEA was 48.7% and NSE was 30.8%. The diagnostic sensitivity is increased to 69.2% by combining CYFRA 21-1 with CEA. Classifying the lung carcinomas following their histologic type, squamous cell carcinomas proved to possess the highest CYFRA 21-1 sensitivity(CYFRA 21-1 47.1%, CEA 23.5%, NSE 11.8%) and the combination with CEA 52.9% could not markedly increase the number of true-positive test results. In adenocarcinoma, CEA possessed the highest sensitivity (81.8%) whereas CYFRA 21-1 was 45.5% and NSE was 9.1%. In small cell carcinoma of lung, NSE possessed the highest sensitivity(81.8%) whereas CYFRA 21-1 was 36.4% and CEA was 54.5%. CONCLUSION: This study indicates that CYFRA 21-1 has a potential as a new marker for squamous cell lung cancer. If histologic type is unknown at the time of primary diagnosis. the combined determination of CYFRA 21-1 and CEA showed the best discrimination between benign and malignant lung disease.
Adenocarcinoma
;
Antibodies, Monoclonal
;
Carcinoma, Small Cell
;
Carcinoma, Squamous Cell
;
Diagnosis
;
Discrimination (Psychology)
;
Enzyme-Linked Immunosorbent Assay
;
Humans
;
Keratin-19
;
Keratins
;
Lung Diseases
;
Lung Neoplasms*
;
Lung*
;
Neoplasms, Squamous Cell
;
Neurons
;
Pilot Projects
;
Sensitivity and Specificity
9.Computerization of bone marrow reporting using full-down menu.
Seong Chul KIM ; Jang Soo SUH ; Han Gil KIM ; Kyung Eun SONG ; Won Kil LEE ; Jay Sik KIM
Korean Journal of Clinical Pathology 1991;11(1):73-78
No abstract available.
Bone Marrow*
10.Identification of Br platelet antigens using modified mixed passive hemagglutination.
Nam Kyung KIM ; Jang Soo SUH ; Jay Sik KIM ; Dal Hyo SONG ; Young Chul OH ; Ki Hong KIM
Korean Journal of Blood Transfusion 1992;3(2):151-157
No abstract available.
Blood Platelets*
;
Hemagglutination*