1.Insecticidal activity of the essential oil of Thymus transcaspicus against Anopheles stephensi
Dargahi Leila ; Razavi-Azarkhiavi Kamal ; Ramezani Mohammad ; Abaee Reza Mohammad ; Behravan Javad
Asian Pacific Journal of Tropical Biomedicine 2014;(z2):589-591
Objective:To investigate the insecticidal activity of the essential oil of Thymus transcaspicus (T. transcaspicus) against Anopheles stephensi (An. stephensi).
Methods:An. stephensi were exposed to 31, 63, 125 and 250 μg/L of essential oil of T. transcaspicus for 24 h.
Results:The most toxicity was observed at 250 μg/L of essential oil with the LC50 values of 134.1 μg/L after 24 h.
Conclusions:The essential oil of T. transcaspicus exhibited strong insecticidal activity against An. stephensi which can be attributed to its constituent especially carvacrol and thymol phenols.
2.Chemokine Receptors Expression in MSCs: Comparative Analysis in Different Sources and Passages.
Asieh HEIRANI-TABASI ; Shirin TOOSI ; Mahdi MIRAHMADI ; Mohammad Amir MISHAN ; Hamid Reza BIDKHORI ; Ahmad Reza BAHRAMI ; Javad BEHRAVAN ; Hojjat NADERI-MESHKIN
Tissue Engineering and Regenerative Medicine 2017;14(5):605-615
MSC-based therapy is providing a cure for degenerative diseases with unmet medical need and usually iliac crest bone marrow (ICBM) are being applied in clinics. Alternative sources, including adipose tissue and reamer/irrigator/ aspirator hold great potential for isolating MCSs. Here, we compared original MSCs features of adipose tissue (Ad-MSCs) and bone marrow of long-bone (RIA-MSCs) or iliac crest, and the expression of chemokine receptors (including CXCR4, CX3CR1, CXCR6, CXCR2, CCR1 and CCR7) in these three sources, which are important in the context of homing. We further investigated the role of SDF-1/CXCR4 axis as a key player in motility of different population of MSCs using Transwell migration assay. All cells exhibited typical MSCs characteristics. However, different MSCs sources expressed different levels of chemokine receptors. Generally, the expression of these chemokine receptors was decreased with increasing passage (P) number from 2 to 3. Interestingly, it was observed that the CXCR4 expression and migration capacity in Ad-MSCs is significantly higher than ICBM and RIA-MSCs in P2. Although our data showed that CXCR4 had highest expression in P2 Ad-MSCs, but it dramatically declined following sub-culturing in the P3. Hence, to improve homing of MSCs by means of chemokine/their receptors axis, the source of isolation and passage number should be considered for clinical applications.
Adipose Tissue
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Bone Marrow
;
Receptors, Chemokine*
;
Stem Cells
Result Analysis
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