OBJECTIVE: Treatment of prostatitis continues to be a challenge. The authors evaluated the culture andsensitivity of expressed prostate secretion of patients with bacterial prostatitis in the local setting. MATERIALS AND METHODS: All patients who were suspected to have prostatitis underwent expressedprostate secretion cultures. All positive cultures were then included in the study. RESULTS: One hundred six (106) culture positive examinations were included in the study.Staphylococcus species were the most common organisms at 55% (59/106), followed by Enterococcusspecies at 17% (19/106). Over-all, Vancomycin was still the antibiotic the organisms were mostsensitive to among all the antimicrobials regardless of organism cultured at 69% (74/106) followedby Ciprofloxacin at 43% (46/106). CONCLUSION The study showed some discrepancy with current known epidemiological studies on theincidence of the organisms causing bacterial prostatitis. It also showed that the current most commonlyused antimicrobial agent (fluroquinolone) may have a high resistance rate that is not acceptable as anempiric treatment hence the investigators recommend at least the use of expressed prostate secretionculture study as guide for the management of prostatitis.