STATEMENT OF PROBLEM: The aim of this study was to study the effects of various surface treatments to a titanium surface on the expression of Runx2 in vitro. MATERIAL AND METHODS: Human Osteosarcoma TE-85 cells were cultured on machined, sandblasted, or anodic oxidized cpTi discs. At various times of incubation, the cells were collected and then processed for the analysis of mRNA expression of Runx2 using reverse transcription-PCR. RESULTS: The expression pattern of Runx2 mRNA was differed according to the types of surface treatment. When the cells were cultured on the untreated control culture plates, the gene expression of Runx2 was not increased during the experiments. In the case of that the cells were cultured on the machined cpTI discs, the expression level was intermediate at the first day, but increased constitutively to day 5. In cells on sandblasted cpTi discs, the expression level was highest in the first day sample and the level was maintained to 5 days. In cells on anodized cpTi discs, the expression level increased rapidly to 3 days, but decreased slightly in the 5-th day sample. CONCLUSION: Different surface treatments may contribute to the regulation of osteoblast function by influencing the level of gene expression of key osteogenic factors.
Durapatite ; Gene Expression ; Humans ; Osteoblasts ; Osteogenesis ; Osteosarcoma ; RNA, Messenger ; Titanium

BACKGROUND: In order to restore severely attrited teeth properly, surgical intervention in the form of a crown-lengthening procedure may be required. And also, proper diagnosis and treatment sequencing is critical to obtain a successful results. Adequate diagnostic wax-up ensures good esthetics and healthy periodontal tissue. CASE DESCRIPTION: This clinical case report describes a diagnostically based protocol for restoration on mandibular anterior teeth with crown lengthening procedure and the treatment of partially edentulous mandible combined with an edentulous maxilla. In addition, the effort to prevent the combination syndrome was described. CLINICAL IMPLICATION: An interdisciplinary diagnosis and examination through visualization of the desired results ensure conservative and more predictable outcome.
Crown Lengthening ; Crowns ; Dietary Sucrose ; Esthetics ; Humans ; Mandible ; Maxilla ; Mouth ; Mouth Rehabilitation ; Tooth

BACKGROUND: Though implant dentistry is very successful and predictable in treatment of patients with destroyed dentition, there are some cases with limitations to implant therapy. In these cases, alternative treatment modality should be considered. CASE DESCRIPTION: A patient with destroyed dentition was rehabilitated with a lateral rotational path removable partial denture. According to the diagnosis, we determined to raise vertical dimension for esthetic and functional restoration. The final restoration was performed after four months of provisional period. CLINICAL IMPLICATION: The edentulous patients with compromised esthetics and functions can be successfully treated with a rotational path removable partial denture through adequate treatment planning and precise laboratory procedure.
Dentistry ; Dentition ; Denture, Partial, Removable ; Esthetics ; Humans ; Mouth ; Mouth Rehabilitation ; Tooth Attrition ; Vertical Dimension

PURPOSE: The aim of this article is to analyze the preference for treatment modality of dentists. MATERIAL AND METHODS: Data of 20,038 patients was involved. Data analysis were done by distribution according to the various kinds of prosthesis, including complete denture, removable partial denture, fixed partial denture, implant-supported dental prosthesis as well as distribution according to the professional titles of the dentists finishing the treatment, including resident and professors. RESULTS: The number of cases of dental prosthesis increased year by year. 61.06% of the patients accepted fixed partial denture restoration. The number of patients who accepted implant supported restoration is also increasing year by year. The number of complete denture, implant-supported dental prosthesis finished by professors was larger than that done by residents, while it was contrary for removable partial denture, fixed partial denture, and the difference was statistically significant (P<.05). CONCLUSION: Professors and residents have some difference in the categorization of prosthesis finished. Fixed partial denture and implant-supported dental prosthesis are preferred.
Dental Prosthesis ; Dental Prosthesis, Implant-Supported ; Dentists ; Denture, Complete ; Denture, Partial, Fixed ; Denture, Partial, Removable ; Humans ; Prostheses and Implants ; Prosthodontics ; Statistics as Topic

STATEMENT OF PROBLEM: It is difficult about assessing the loosening tendency of conical connection type implant after cyclic loading with diamond like carbon coating one-piece abutments, and also about the retightening effect. PURPOSE: This study was performed to investigate the influence of one-piece abutment screw retightening after 5.0X10(4) cyclic loading and Diamond like coating MATERIAL AND METHODS: Thirty two ITI implant were divided to 4 groups. Group 1,3-titanium abutment, group 2,4 - diamond like carbon coated abutment. Group 1,2 - 20.0X10(4) cyclic loading after 5.0X10(4) cyclic loading, Group 3,4- after 20.0X10(4) Cyclic loading. After cyclic loading, periotest values were taken and removal torque values of abutments were measured with a digital torque gauge. RESULTS: 1. The removal torque of group 2 after 5.0X10(4) cyclic loading is slightly greater than the other groups but not significantly higher than others (P>0.05). 2. The final removal torque values after 20.0X10(4) cyclic loading of group 1 is bigger than group 3, and group 2 is bigger than group 4, but not significantly higher (P>0.05). 3. The final removal torque values after 20.0X10(4) cyclic loading of all groups are not significantly different (P>0.05).
Carbon ; Diamond ; Torque

The color stability of current prosthetic composites is unknown, even though the quality of com-posite materials has been improving. This study examined the intrinsic color stability of various current prosthetic resins (ceramic-polymers) after an accelerated aging process and the extrinsic color stability after immersion in a coffee solution. By comparing the amount of dis-coloration after aging with that without aging, the effect of the accelerated aging process on external discoloration could be evaluated. Three current prosthetic composites (Artglass, Targis, Sculpture), one light polymerized direct composite (Z100) and one dental porcelain control (Ceramco) were assessed. The col-or changes (delta E) of all the specimens were determined using the CIE L*a*b* color order system with a reflected spectrophotometer. The results were as follows: 1. The prosthetic composite materials subjected to the accelerated aging test showed no sig-nificant difference in color changes (p> .05). 2. In the coffee solution immersion test after the aging process, the color changes of the Targis and Artglass groups were not different from that of the Z100 group, which showed the highest color change. 3. In the immersion only test, a significantly high color change was observed in the sculpture glazing group. 4. The aging process influenced on the color changes more in the Targis, Artglass and Z100 groups than in the Sculpture and Ceramco groups.
Aging* ; Coffee* ; Dental Porcelain ; Immersion* ; Polymers ; Sculpture

The purpose of this study was to compare and evaluate the stress distribution and displacement developed in the abutment teeth and residual ridge area by madibular unilateral distal extension removable partial denture with 2 different retainer designs. The retainers on right and left canine and right 2nd molar were Akers clasp in one model and telescopic crown in the other model. The stress distribution of abutment teeth and residual ridge area on two model were compared and analyzed with 3-dimensional finite element method. 150N and 400N forces were applied vertically, 30 degree and horizontally on the central fossa area of left 1st molar of the removable partial denture, and then stress distribution patterns were analyzed and compared. The results were as follows 1. As the magnitude and angulation of applied force were increased, the magnitude of stress on the right and central residual ridge area and the right canine of the telescopic type increased and comparing to those of the Akers clasp type. 2. As the magnitude and angulation of applied force were increased, the mesial direction of displacement on the right residual ridge area and the right tooth of the telescopic type increased and the distal direction of displacement on left residual ridge area and the left canine increased comparing to those of Akers clasp type. 3. As the vertical force was applied, the distal direction of the displacement of the right tooth were greater and that of the left canine was smaller and the upward displacement of the right canine was greater in telescopic partial denture than those of Akers clasp type. 4. As the 30 degree force was applied, the mesial direction of the displacement of the right tooth were greater and the distal direction of the displacement of the left canine was smaller and the upward displacement of the right canine was greater in telescopic partial denture than those of Akers clasp type. In the horizontal force the results were same in right area tooth but the distal direction of displacement was greater in left canine. 5. In both removable partial dentures, as the magnitude and degree of force were increased, the stress and displacement were increased. The compressive force was dominative than the tensile force. 6. In both removable partial dentures, the magnitude of stress was greater on mucosal tissue area than that of the alveolar bone area on distal extension residual ridge area but the result was reversed on anterior residual ridge area. The displacement was always greater on mucosal tissue area than that of alveolar bone area.
Crowns ; Denture, Partial ; Denture, Partial, Removable ; Molar ; Mucous Membrane ; Tooth*

PURPOSE: This study investigated the influence of bone quality and surgical technique on the implant stability quotient (ISQ) value. In addition, the influence of interfacial bone quality, directly surrounding the implant fixture, on the resonance frequency of the structure was also evaluated by the finite element analysis. MATERIALS AND METHODS: Two different types of bone (type 1 and type 2) were extracted and trimmed from pig rib bone. In each type of bone, the same implants were installed in three different ways: (1) Compaction, (2) Self-tapping, and (3) Tapping. The ISQ value was measured and analyzed to evaluate the influence of bone quality and surgical technique on the implant primary stability. For finite element analysis, a three dimensional implant fixture-bone structure was designed and the fundamental resonance frequency of the structure was measured with three different density of interfacial bone surrounding the implant fixture. RESULTS: In each group, the ISQ values were higher in type 1 bone than those in type 2 bone. Among three different insertion methods, the Tapping group showed the lowest ISQ value in both type 1 and type 2 bones. In both bone types, the Compaction groups showed slightly higher mean ISQ values than the Self-tapping groups, but the differences were not statistically significant. Increased interfacial bone density raised the resonance frequency value in the finite element analysis. CONCLUSION: Both bone quality and surgical technique have influence on the implant primary stability, and resonance frequency has a positive relation with the density of implant fixture-surrounding bone.
Bone Density ; Finite Element Analysis ; Ribs

PURPOSE: The aim of this study was to investigate a comparison of implant bone bed preparation with Er,Cr:YSGG laser and conventional drills on the relationship between implant stability quotient (ISQ) values and implant insertion variables. MATERIALS AND METHODS: Forty implants were inserted into two different types of pig rib bone. One group was prepared with conventional drills and a total of 20 implants were inserted into type I and type II bone. The other group was prepared with a Er,Cr:YSGG laser and a total of 20 implants were inserted into type I and type II bone. ISQ, maximum insertion torque, angular momentum, and insertion torque energy values were measured. RESULTS: The mean values for variables were significantly higher in type I bone than in type II bone (P < .01). In type I bone, the ISQ values in the drill group were significantly higher than in the laser group (P < .05). In type II bone, the ISQ values in the laser group were significantly higher than in the drill group (P < .01). In both type I and type II bone, the maximum insertion torque, total energy, and total angular momentum values between the drill and laser groups did not differ significantly (P > or = .05). The ISQ values were correlated with maximum insertion torque (P < .01, r = .731), total energy (P < .01, r = .696), and angular momentum (P < .01, r = .696). CONCLUSION: Within the limitations of this study, the effects of bone bed preparation with Er,Cr:YSGG laser on the relationship between implant stability quotient (ISQ) values and implant insertion variables were comparable to those of drilling.
Mandrillus ; Ribs ; Salicylates ; Torque

STATEMENT OF PROBLEM: A biochemical approach for surface modification has offered an alternative for physicochemical and morphological methods to obtain desirable bone-implant interfaces. PURPOSE: The purpose of the present study was to investigate cell responses to poly (D,L-lactide-co-glycolide) (PLGA)/1alpha, 25-(OH)2D3 coating with reference to cellular proliferation and differentiation in vitro. MATERIAL AND METHODS: 96 titanium discs were fabricated and divided into four groups. Group 1 was anodized under 300 V as control. Group 2, 3 and 4 were anodized then coated with 3 ml PLGA/1alpha, 25-(OH)2D3 solutions. Amount of the solutions were 2 ul, 20 ul and 200ul respectively. The osteoblast-like Human Osteogenic Sarcoma (HOS) cells were seeded and cultured for 1, 3 and 7 days. MTSbased cell proliferation assay and ALPase activity test were carried out. RESULTS: PLGA nanoparticles were observed as fine, smooth and round and HOS cells attached to the anodized surfaces through strand-like and sheet-like filopodia. After 3 days of culture, the dendritic filopodia were exaggerated and sheet-like cytoplasmic projections covered the coated titanium surfaces. After 3 days of culture, all of the groups showed increased cellular proliferation and the lowest proliferation rate was measured on group 2. Higher amount of incorporated 1 alpha, 25-(OH)2D3 (Group 3 and 4) improved cellular proliferation but the differences were not significant statistically (P > .05). But they increased the rate of ALP activities than the control group at day 3 (P < .05). CONCLUSION: Biodegradable PLGA nanoparticles incorporated with vitamin D metabolite positively affected proliferation and differentiation of cells on the anodized titanium surface.
Calcitriol ; Cell Proliferation ; Cytoplasm ; Humans ; Lactic Acid ; Nanoparticles ; Osteosarcoma ; Polyglycolic Acid ; Pseudopodia ; Seeds ; Titanium ; Vitamin D