The sunscreening effect can be varied according to the vehicles. Therefore the purpose of this study is to compare the effect of vehicles(bases) on sun protection in p-aminobenzoic acid, cinnamate and benzophenone sunscreens with same concentration (2.5%), We included ointment (white petrolatum), cream (hydrophilic), milky lotion and alcohol as the sunscreen vehicles. The test results can be summarized as follows: We could not recognize a sunscreening effect of sunscreen vehicles which did not contain sunscreening agent. In the case of p-aminobenzoic acid, the mean sun protection factor was higher in the sequence of cream, milky lotion, alcohol, ointment, each showing 9, 95+/-3.67, 8.09+/-2.56, 5.14+/-1.45, 4.35+/-1.46 respectively. In the case of cinnamate, the mean sun protection factor was higher in the sequence of cream, ointment, milky lotion, alcohol, each showing 6 46+/-1.89, 5.42+/-1.49, 4.82+/-1.84, 4.05+/-1.45 respectively. 4. In the case of benzophenone, the mean sun protection factor was higher in the sequence of cream, alcohol, ointment, milky lotion, each showing 5 .26+/-1.56, 4.94+/-1.24, 4.56+/-1.71, 4.18+/-1.23 respectively.
4-Aminobenzoic Acid ; Skin* ; Solar System ; Sun Protection Factor ; Sunscreening Agents

We have observed the dark effect of 8-methoxypsoralen(8-M(P) on the viability and DNA synthesis in human lymphocyte cultures after stimulaticn with phytohemagglutinin (PHA) in the absence of ultraviolet A radiation. The concentratioiis of 8-MOP was 0.5-32 ug/ml. We have also measured the LDH activity in supernatant. of lymphocyte cultures treated with 8-MOP. The results were as follows: 1. There was no 8-MOP dose-dependent decrease in the viability of lymphocytes up to 8- MOP 32pg/ml. 2. There was a 8-MOP dose-dependent decrease in PHA-induced DNA synthesis of lymphocytes from the concentration of 8-MOP 2 ug/ml. 3. There was a time-dependent decrease in PHA-induced DNA synthesis of lymphocytes at the concentration of 8-MOP 32 ug/ml. 4. There was no LDH release in supernatant of lymphocyte ciltu es after incubation with 8-MOP up to 8-MOP 32ug/ml.
Animals ; DNA ; Ear* ; Humans ; Lymphocytes ; Methoxsalen ; Mice ; Mice, Inbred C57BL* ; Photochemotherapy* ; Skin*

No abstract available.

The serologic testa for syphilis including VDRL and FTA-abs tests have been carried out in adult VISA applicants for emigration and bIood donors from February, 1977 to May, 1978. The results are summarized as followa: l. In 3,393 VISA applicants the reactive rate af VDRL test was 2.9%, and th biologic false positive rate of VDRL test was 5.1% using the FTA-abs test as the atandared. 2. VDRL test showed a positive rate of 2.3% in 6,220 blood donors. 3. The quantitative test of VDRL resulted in low titer belaw 1:4 in 93.9% of VDRL reactive VISA applicants. From the results it is clear that the prevalence of syphilis is grariually increasing recently compared to the late 1960s and early 1970s.
Adult ; Blood Donors ; Emigration and Immigration ; Fluorescent Treponemal Antibody-Absorption Test ; Humans ; Prevalence ; Skin Diseases* ; Syphilis ; Tissue Donors ; Zinc*

The object of this study is to evaluate the effects of repeat,ed PUVA applications on epidermal melanocytes. The changes in the number of melanocyes is compared to the frequency of PUVA applications. 26 adult male C57BL mice were used. About an hour after appliction of 0.1% solution of 8-methoxypsoralen, UVA was delivered two or four times a weels on both ears of mice with blacklight fluorescent tube l,'Waldmann UV-800). Weekly examinations of melanocytes were made for 4 weeks on split epidermal sheets treated with DOPA solution. The results were as follows. 1. Repeated PUVA applications revealed a significant increase in the number of melanocytes. With PUVA, two times a week, the number of me anocytes was increased from 83.2+/-7.2/mm2 (unir radiated ear) to 250.6+/-34.7/mm2 after ore week and 525.5+/-66.7/mm2 after 4 weeks. With PUVA, four times a week, the number of melanocytes was increased from 83.2+/-7.. 2/mm2 (unirradiated ear) to 437.0+/-74.0/mm2 after one week and 686.3+/-27.8/mm2 after 4 weeks. 2. PUVA, four times a wcek, elicited higher melanocyte density than PUVA, two times a week, under the same period of treatment. 3. After two weeks, the same amount of irradiation increased the number of melanocytes more in PUVA, four times a week than in PUVA, t,wo times a week.
Adult ; Animals ; Dihydroxyphenylalanine ; Ear ; Humans ; Male ; Melanocytes* ; Methoxsalen ; Mice ; Mice, Inbred C57BL*

No abstract available.
DNA* ; Humans* ; Lymphocytes*

Calcipotriol, a synthetic analogue of calcitriol(1,25-dihydroxyvitamin D) is as active as calcitriol in inducing cell differentiation and inhibiting cell pralifiration but at least 100 times less active than calcitriol in its effect on calcium metabolism. We report a case of psoriasis in a 28 year old male who was treated with calcipotriol ointment on the right forearm. The therapeutic effect of calcinoipotriol ointment was compared with desoxymethason, cream applied on the left forearm. Both topical agents reduced equally the degrees of scale, erythema and thickness of the lesions. We observed that the calcipotriol ointment was effective in the treatment of psoriasis n this case.
Adult ; Calcitriol ; Calcium ; Cell Differentiation ; Erythema ; Forearm ; Humans ; Male ; Metabolism ; Psoriasis*

Photosensitive psoriasis is a rare disease defined as psoriasis in which the lesions deteriorate or new lesions develop after sun exposure. It should be differentiated from other photosensitive conditions that may be a vated or confused on sun-exposure. Half of the patients have a history of polymorphic light eruption(PMLE), whieh several weeks later develops into psoriasis lesions and the others have no history of preceding PMLE reaction. PUVA therapy is recommended for the treatment of choice. In this report, we describe two cases of photosensitive psoriasis. A 51-year-old woman without underlying psoriasis suffered from erythemato-squamous papular lesions on the face, neck and dorsum of hands after sun-exposure. She revealed a lowered minimal erythema dose(MED) for UVA which was confused with a photosensitive disease. But characteristic psariatic plaques on the elbow, knee and palm and histologic findings made the diagnosis photosensitive psoriasis. She showed a good result to cyclosporine therapy. The other patient, a 44-year-old woman with underlying psoriasis, experienced an exacerbation with preceding PMLE and showed a lowered erythema threshold for UVB. She was treated with sun-screen and topical corticosteroids.
Adrenal Cortex Hormones ; Adult ; Cyclosporine ; Diagnosis ; Elbow ; Erythema ; Female ; Hand ; Humans ; Knee ; Middle Aged ; Neck ; Psoriasis ; PUVA Therapy ; Rare Diseases ; Solar System

Three cases of pseudoxanthoma elasticum are presented and the literatures are reviewed. Two cases were presented with the marked cutaneous manifestation of this condition and angioid streak. One case was presented with only the marked skin lesion of this condition on both axilla and neck. Typically involved skin was shown to be speckled with pea-sized yellowish numorous papules. Typical elastic tissue degeneration was found in the involved skin. No genetic background was discovered in the patients.
Angioid Streaks ; Axilla ; Elastic Tissue ; Humans ; Neck ; Pseudoxanthoma Elasticum* ; Skin

The present study was undertaken to inveatigate whether contaet sensitivity was decreased or not in patients with atopic dermatitis, and to investigate phagocytic function of neutrophils in patients with atopic dermatitis. 1. Dinitrochlorobenzene (DNCB), a compound which uniformly induce hypersensitivity in normal individuals, had been used in the preaent study to investigate cutaneous anergy in 16 patients with atopic dermatitis. Sensitizing does of DNCB (2,000 microgram) was applied and examined at 7th and 14th day for a spontaneous flare. In the absence of spontaneous flare, challenge dose (50microgram) was reapplied and examined. Seven of the 16 cases (43.8%.) had positive reaction to DNCB. These result showed decreased contact sensitivity in patients with atopic dermatitis, which suggested some defect in the cell-mediated immunity in patients with atopic dermatitis. 2. There were some evidence that susceptibility to certain infection was increased in patients with atopic dermatitis. For investigating killing function in phagoeytosis of neutrophils in patients with atopic dermatitis, nitroblue tetrazolium dye reduction (NBT) and stimalated NBT test had performed in 35 cases of atopic dermatitis and 20 cases of healthy control. 1) There was no significant difference in the mean percentage and absolute number of NBT positive neutrophils between whole group of atopic dermatitis (8. 4+4. 9%, 301.2+225.6) and control group (6. 9+2. 9%, 246.2+143. 8). 2) The mean percentage and absolute number of stmulated NBT poeitive neutrophils in whole group of atopic dermatitis (19.6+7.3%, 702. 1+377. 2) did not differ significantly from control group (22.7+5.3%, 812.2+382.2). 3) There was no significant difference in the mean value of NBT aad stimulated NBT test in comparing the mild groud, moderate group and severe group with control group. These resuIts suggested that killing function in phagocytosis of neutrophils in patients with atopic dermatitis was not impaired.
Dermatitis, Atopic* ; Dermatitis, Contact ; Dinitrochlorobenzene* ; Homicide ; Humans ; Hypersensitivity ; Immunity, Cellular ; Neutrophils ; Nitroblue Tetrazolium ; Phagocytosis