1.The Usefulness of Calretinin Immunohistochemistry for Rectal Suction Biopsy to Diagnose Hirschsprung’s Disease.
Jaeyeop JEONG ; Sang Pyo KIM ; Eunyoung JUNG ; Soon Ok CHOI
Journal of the Korean Association of Pediatric Surgeons 2016;22(2):23-28
PURPOSE: Hirschsprung’s disease (HD) is a congenital intestinal disorder with absence of ganglion cells in the intestinal muscle and submucosa. Diagnosis is based on histopathological study such as H&E, and acetylcholinesterase (AchE) immunohistochemistry. Calretinin immunohistochemistry was introduced as a new diagnostic method against limitations of other staining. The aim of this study is to investigate the usefulness of calretinin immunohistochemistry for the diagnosis of HD compared to H&E and AchE. METHODS: Ten patients with HD and 22 non-HD patients were included in the study. H&E staining, AchE and calretinin immunohistochemistry were performed in all 32 patients. All slides were evaluated by same single pathologist and the diagnostic value was calculated for each H&E stain, AchE immunohistochemical staining, and calretinin immunohistochemical staining. RESULTS: Calretinin method had sensitivity of 100% and specificity of 100% for diagnosis of HD. Its diagnostic accuracy was 100%. AchE staining showed 100% of specificity and 80% of sensitivity. Diagnostic accuracy of H&E staining was 56.3%. CONCLUSION: We concluded that calretinin immunohistochemistry is a very useful and valuable method to diagnosis HD patient.
Acetylcholinesterase
;
Biopsy*
;
Calbindin 2*
;
Diagnosis
;
Ganglion Cysts
;
Hematoxylin
;
Hirschsprung Disease
;
Humans
;
Immunohistochemistry*
;
Sensitivity and Specificity
;
Suction*
2.Endonasal Septal Perforation Repair: Free Mucosal Graft With Lyoplant® Bioscaffold
Hee Jun YI ; Jaeyeop SIM ; Jae Hyuk CHOI ; Nayeon CHOI ; Jeong Hong KIM
Journal of Rhinology 2021;28(3):180-185
Nasal septal perforation (NSP) is a common complication of nasal surgery and can cause nasal obstruction, crust, and epistaxis. Many surgical methods have been introduced for repair of NSP, among which mucosal flap and artificial dermis have been widely used. However, mucosal graft can shrink and migrate and is difficult to fix at the perforation site. Mucosal advancement flap requires a wide extent of septal mucosa dissection, and artificial dermis can cause nasal obstruction because of its bulkiness and lower biocompatibility than autologous mucosa. To overcome these problems, we reported successful outcomes in 4 cases of small NSP by free mucosal graft with bioscaffold.