1.The Management of Heparin-induced Thrombocytopenia with Thrombosis That Developed after Aortic Dissection Surgery.
JaeBum KIM ; Nam Hee PARK ; Sae Young CHOI
The Korean Journal of Thoracic and Cardiovascular Surgery 2010;43(5):538-541
Heparin-induced thrombocytopenia (HIT) is a clinicopathologic condition and adverse drug reaction caused by immunoglobulin G (IgG) antibodies directed against the heparin-platelet factor 4 complex. HIT with thrombosis (HITT) could lead to limb amputation, stroke, myocardial infarction, and death. We report on the successful management of a HITT patient with argatroban therapy.
Amputation
;
Antibodies
;
Drug Toxicity
;
Extremities
;
Heparin
;
Humans
;
Immunoglobulin G
;
Myocardial Infarction
;
Pipecolic Acids
;
Stroke
;
Thrombocytopenia
;
Thrombosis
2.Ruptured Penetrating Atherosclerotic Ulcer of the Aortic Arch in a Patient with a Previous History of Replacing the Descending Thoracic Aorta.
JaeBum KIM ; Sae Young CHOI ; Nam Hee PARK
The Korean Journal of Thoracic and Cardiovascular Surgery 2008;41(5):647-650
Aortic disease usually recurs after aortic surgery in the form a new aneurysm distal to the previous operation site, and finding a penetrating atherosclerotic ulcer proximal to the previous operation site has rarely been reported. We report here on a case of successful patch repair of a ruptured penetrating ulcer in the distal aortic arch, and this developed late after replacement of the descending thoracic aorta.
Aneurysm
;
Aorta, Thoracic
;
Aortic Diseases
;
Atherosclerosis
;
Humans
;
Ulcer
3.Intracardiac Migration of a Renal Stent from the Left Renal Vein to the Right Ventricle during the Treatment of Nutcracker Syndrome: A case report.
JaeBum KIM ; Sae Young CHOI ; Nam Hee PARK ; Dong Yoon KUM ; Hoon PARK ; Eun Ah HWANG
The Korean Journal of Thoracic and Cardiovascular Surgery 2010;43(1):100-103
We report her on a rare case of a renal stent that migrated into the right ventricle in a patient with nutcracker syndrome. A 29-year-old woman was admitted to the hospital and she was suffering from flank pain. The computed tomography of the abdomen demonstrated that the left renal vein was compressed between the abdominal aorta and the superior mesenteric artery (nutcracker syndrome). A self expandable stent was placed across the left renal vein for treating her nutcracker syndrome. The next day after the procedure, the follow up chest radiograph showed that the displaced stent had migrated into the right ventricle. After percutanous endovascular stent removal had failed, the stent was ultimately removed by performing cardiac surgery. At the 6th postoperative month, there have been no abdominal or cardiac symptoms.
Abdomen
;
Adult
;
Aorta, Abdominal
;
Blood Vessel Prosthesis
;
Female
;
Flank Pain
;
Follow-Up Studies
;
Heart Ventricles
;
Humans
;
Mesenteric Artery, Superior
;
Prosthesis Failure
;
Renal Veins
;
Stents
;
Stress, Psychological
;
Thoracic Surgery
;
Thorax
;
Vascular Diseases
4.Synovectomy of the Rheumatoid Knee Using Intra-articular Injection of 165Dy Hydroxide Macroaggregates
Sugjun KIM ; Sooyoung LEE ; Daegeun JEON ; Jongseok LEE ; Taewan KIM ; Donghwan CHUNG ; Hyunsoo PARK ; Sungwoon HONG ; Sangmoo LIM ; Changwoon CHOI ; Seongyou KIM ; Daehyun YOO ; Sangcheol BAE ; Inhong LEE ; Sungsoo JUNG ; Jaebum JUN
The Journal of the Korean Orthopaedic Association 1996;31(5):1013-1017
165Dy Hydroxide Macroaggregates(165Dy HMA) has a short half life(2.3 hours) and a size range of 3-5µm that give the advantage of reduced leakage and a shorter hospital stay. This report will show the results of a prospective open study on the efficacy and safety of 165Dy HMA in 178 knees of 141 patients with chronic synovitis refractory to conventional antirheumatic therapy. The final global assessment was classified as good, fair or poor. Extra-articular leakage of 165Dy HMA was determined by the scintigraphic evaluation of liver, groin and knee joints. The optimum radiation dose was 250 mCi. The mean follow up periods were 32.4(14-112) weeks. Thirty seven percent of the knees showed good results, 48% fair results and 15% poor results. In the knees with stage I radiographic changes, 82% showed improvement including 32% of the patients with good results. In the knees with stage II radiographic changes, 90% showed improvement including 42% of the patients with good results. The mean period of improvement for the 158 knees that responded to treatment was 41.4(24-106) weeks. Leakage of radioactivity from the injected joint was minimal. Adverse reactions were rare(radiation burn : 4 cases, transient postinjection swelling : 14 cases). In conclusion, 165Dy HMA radiation synovectomy is a safe and useful therapy for chromic synovitis of the rheumatoid knees.
Arthritis, Rheumatoid
;
Burns
;
Follow-Up Studies
;
Groin
;
Humans
;
Injections, Intra-Articular
;
Joints
;
Knee Joint
;
Knee
;
Length of Stay
;
Liver
;
Prospective Studies
;
Radioactivity
;
Synovitis
5.Fabrication and Characterization of Graphene Oxide-Coated Plate for Efficient Culture of Stem Cells
Danbi PARK ; Jaebum PARK ; Jihoon LEE ; Chang Jae SHIM ; Min Sung KIM ; Tae Yong LEE ; Jeong Ok LIM
Tissue Engineering and Regenerative Medicine 2021;18(5):775-785
BACKGROUND:
For stem cell applications in regenerative medicine, it is very important to produce high-quality stem cells in large quantities in a short time period. Recently, many studies have shown big potential of graphene oxide as a biocompatible substance to enhance cell growth. We investigated if graphene oxide-coated culture plate can promote production efficiency of stem cells.
METHODS:
Three types of graphene oxide were used for this study. They are highly concentrated graphene oxide solution, single-layer graphene oxide solution, and ultra-highly concentrated single-layer graphene oxide solution with different single-layer ratios, and coated on cell culture plates using a spray coating method. Physiochemical and biological properties of graphene oxide-coated surface were analyzed by atomic force microscope (AFM), scanning electron microscope (SEM), cell counting kit, a live/dead assay kit, and confocal imaging.
RESULTS:
Graphene oxide was evenly coated on cell culture plates with a roughness of 6.4 * 38.2 nm, as measured by SEM and AFM. Young’s Modulus value was up to 115.1 GPa, confirming that graphene oxide was strongly glued to the surface. The ex vivo stem cell expansion efficiency was enhanced as bone marrow-derived stem cell doubling time on the graphene oxide decreased compared to the control (no graphene oxide coating), from 64 to 58 h, and the growth rate increased up to 145%. We also observed faster attachment and higher affinity of stem cells to the graphene oxide compared to control by confocal microscope.
CONCLUSION
This study demonstrated that graphene oxide dramatically enhanced the ex vivo expansion efficiency of stem cells. Spray coating enabled an ultra-thin coating of graphene oxide on cell culture plates. The results supported that utilization of graphene oxide on culture plates can be a promising mean for mass production of stem cells for commercial applications.
6.Fabrication and Characterization of Graphene Oxide-Coated Plate for Efficient Culture of Stem Cells
Danbi PARK ; Jaebum PARK ; Jihoon LEE ; Chang Jae SHIM ; Min Sung KIM ; Tae Yong LEE ; Jeong Ok LIM
Tissue Engineering and Regenerative Medicine 2021;18(5):775-785
BACKGROUND:
For stem cell applications in regenerative medicine, it is very important to produce high-quality stem cells in large quantities in a short time period. Recently, many studies have shown big potential of graphene oxide as a biocompatible substance to enhance cell growth. We investigated if graphene oxide-coated culture plate can promote production efficiency of stem cells.
METHODS:
Three types of graphene oxide were used for this study. They are highly concentrated graphene oxide solution, single-layer graphene oxide solution, and ultra-highly concentrated single-layer graphene oxide solution with different single-layer ratios, and coated on cell culture plates using a spray coating method. Physiochemical and biological properties of graphene oxide-coated surface were analyzed by atomic force microscope (AFM), scanning electron microscope (SEM), cell counting kit, a live/dead assay kit, and confocal imaging.
RESULTS:
Graphene oxide was evenly coated on cell culture plates with a roughness of 6.4 * 38.2 nm, as measured by SEM and AFM. Young’s Modulus value was up to 115.1 GPa, confirming that graphene oxide was strongly glued to the surface. The ex vivo stem cell expansion efficiency was enhanced as bone marrow-derived stem cell doubling time on the graphene oxide decreased compared to the control (no graphene oxide coating), from 64 to 58 h, and the growth rate increased up to 145%. We also observed faster attachment and higher affinity of stem cells to the graphene oxide compared to control by confocal microscope.
CONCLUSION
This study demonstrated that graphene oxide dramatically enhanced the ex vivo expansion efficiency of stem cells. Spray coating enabled an ultra-thin coating of graphene oxide on cell culture plates. The results supported that utilization of graphene oxide on culture plates can be a promising mean for mass production of stem cells for commercial applications.