Inositol 1,4,5-triphosphate(InsP,) is a second messenger for obilizing intracellular Cal'. It can be dephosphorylated by soluble and particulate forms on InsP, 5-phosphatase, or phosphorylated to produce inositol 1,3,4,5-tetrakisphosphate(InsP,) by InsP, 3-kinase. These enzymes represent possible targets for the regulation of the InsP,AnsP. signal. InsP, 3-kinase which catalyses th ATP-dependent phosphorylation of InsP, was purified from bovine brain tissue. All operation were carried out at 41C. Fresh tissure was homogenized and centrifuged. The supernatant was pooled. Proteins were precipitated from 10% polyethylene glycol, and suspended solution was applied to DEAE cellulose column for chromatography. As the result of above procedure, two isozymes of InsP, 3-kinase, I and U were obtained. Each isozyme was applied to Matriz green gel, Calmodulin-Affigel 15 column and subsequent phenyl-TSK HPLC column. Specific activites(SA) and fold of puriety were observed at each purification step of chromatography. At DEAE cellulose chromatography, SA were 1, 0.6 and 11, 4.8 nM/min/mg, and folds
Brain* ; Catalysis ; Chromatography ; Chromatography, DEAE-Cellulose ; Chromatography, High Pressure Liquid ; DEAE-Cellulose ; Inositol* ; Isoenzymes ; Phosphorylation ; Phosphotransferases* ; Polyethylene Glycols ; Second Messenger Systems

The suprapatellar plica is a synovial membrane that lies between the suprapatellar pouch and the true knee joint, but pathologic suprapatellar plica has been reported not frequently. We experienced one case of arch type of pathologic suprapatellar plica which was excised by arthroscopic technique, and report it with review of literature.
Knee Joint ; Synovial Membrane

No abstract available.
Menisci, Tibial*

BACKGROUND: Gonorrhea is one of the most common venereal diseases in the world. Antibioticresistance development has been an issue with the penicillinase-producing Neisseria gonorrhoeae(PPNG) development. It is possible for PPNG to be resistant to other medications. Therefore, it is significant to determine its frequency rate and its disposition. OBJECTIVES: In this surveillance, we examined overall development frequency of PPNG from 1981 ti 1998. Comparing current five years' frequency rate with that of previous five year, we predicted possible development rate and described the procedures the physicians must take into account in gonorrhea treatment. METHODS: We examined the development frequency of PPNG in male patients of the venereal disease clinic of Jung-gu Community Health Center in Seoul from 1981 to 1998. Also, we analyzed the overall tendency with time series analysis using statistics package, SPSS 7.5.Grouping the years in five-year units, development frequency of PPNG of current five years (1994-1998) was compared with that of previous years. RESULTS: For the result of monthly analysis of frequency rate of PPNG development from 1981 to 1998, the rate tendency came out with a sligh rise (slope : 0.15). And for the result of comparison of grouped years, in current five years, its rate was 51.6%, marking a significant decline from that of the previous five years, which was 57.2% (p-value : <0.0001). CONCLUSION: The rate of current five years was reported to have declined from the previous years. However, the rate is still over 50%. Therefore, the government should continue the PPNG surveillance with test of sensitivity of the medications in use. Moreover, to eliminate gonorrhea, the education on high risk groups should continue and physicians should use a dose of adequate antibiotics in treatment.
Anti-Bacterial Agents ; Community Health Centers* ; Education ; Gonorrhea ; Humans ; Male ; Neisseria gonorrhoeae* ; Neisseria* ; Seoul* ; Sexually Transmitted Diseases

No abstract available.
Bronchiectasis*

No abstract available.

A 26 year old female presented complaining of a pea-sized nodule on her left lower eyelid for 10 years duration. Histopathological study showed several cysts in the dermis surrounded by secretory cells. The cysts were filled with decapitatory secretions. Positive staining with carcinoembrynic an tigen(CEA) was detected by immunoperoxidase technique. These clinical and histopathological findings suggest apocrine hidrocystoma developed on the left lower eyelid.
Adult ; Dermis ; Eyelids ; Female ; Hidrocystoma* ; Humans ; Immunoenzyme Techniques

BACKGROUND: The number of nongonococcal urethritis(NGU) cases is exceeding more and more that of Gonorrhea in developeing nations and some of the developing countr es such as Korea. The best evidence for an etiologic role for Trichomonas Vaginalis is in nongonococcal urethritis. OBJECTIVE: We compared the effects of Tetracycline to Tetracycine and Metronidazole in the treatment of male patients with nongonococcal urethritis and wished to find out the incidence of Trichomon-as Vaginalis in male patients with nongonocaccal urethritis indirectly. METHOD: From January, ],989 to December, 1992, three hundred male patients with nongococcal urethritis(NGU) at the Venreal Disease Clinic of Choong-Ku Publi Health Center in Seoul were allocated randomly into one of two treatment regimens. and one hundred sixty eight patients were followed. RESULTS: Eighty(83.4%) of ninty six patients treated with Tetrac; cline, 500mg, PO, q.i.d. for 14 days were cured. Sixty two(86.2%) of seventy two patients treatec with Tetracycline, 500mg, PO, q.i. d. for 14 days and Metronidaole. 2.0gm, PO, for 1 day were cured. There is no significant different between the 2 groups in treated rates(P<0.05). CONCLUSION: It suggests that male patients with nongonococcal ure1hritis due to trichomoniasis are rare in Korea. This may be die to the fact that the incidence of trichorioniasis has been declining since the 1960s. It also suggests not only the need for further studies on tool is disease but also more studies on other causes of nongonocoqeal urethritis, its treatment and epidernio.ogical study.
Gonorrhea ; Humans ; Incidence ; Korea ; Male* ; Metronidazole* ; Seoul ; Tetracycline* ; Trichomonas vaginalis ; Urethritis

No abstract available.
Drug Therapy* ; Humans ; Uterine Cervical Neoplasms*

No abstract available.
Carcinoma, Hepatocellular* ; Cell Line* ; Hepatoblastoma* ; Humans*