Inositol 1,4,5-triphosphate(InsP,) is a second messenger for obilizing intracellular Cal'. It can be dephosphorylated by soluble and particulate forms on InsP, 5-phosphatase, or phosphorylated to produce inositol 1,3,4,5-tetrakisphosphate(InsP,) by InsP, 3-kinase. These enzymes represent possible targets for the regulation of the InsP,AnsP. signal. InsP, 3-kinase which catalyses th ATP-dependent phosphorylation of InsP, was purified from bovine brain tissue. All operation were carried out at 41C. Fresh tissure was homogenized and centrifuged. The supernatant was pooled. Proteins were precipitated from 10% polyethylene glycol, and suspended solution was applied to DEAE cellulose column for chromatography. As the result of above procedure, two isozymes of InsP, 3-kinase, I and U were obtained. Each isozyme was applied to Matriz green gel, Calmodulin-Affigel 15 column and subsequent phenyl-TSK HPLC column. Specific activites(SA) and fold of puriety were observed at each purification step of chromatography. At DEAE cellulose chromatography, SA were 1, 0.6 and 11, 4.8 nM/min/mg, and folds
Brain* ; Catalysis ; Chromatography ; Chromatography, DEAE-Cellulose ; Chromatography, High Pressure Liquid ; DEAE-Cellulose ; Inositol* ; Isoenzymes ; Phosphorylation ; Phosphotransferases* ; Polyethylene Glycols ; Second Messenger Systems

The suprapatellar plica is a synovial membrane that lies between the suprapatellar pouch and the true knee joint, but pathologic suprapatellar plica has been reported not frequently. We experienced one case of arch type of pathologic suprapatellar plica which was excised by arthroscopic technique, and report it with review of literature.
Knee Joint ; Synovial Membrane

No abstract available.
Menisci, Tibial*

BACKGROUND: Gonorrhea is one of the most common venereal diseases in the world. Antibioticresistance development has been an issue with the penicillinase-producing Neisseria gonorrhoeae(PPNG) development. It is possible for PPNG to be resistant to other medications. Therefore, it is significant to determine its frequency rate and its disposition. OBJECTIVES: In this surveillance, we examined overall development frequency of PPNG from 1981 ti 1998. Comparing current five years' frequency rate with that of previous five year, we predicted possible development rate and described the procedures the physicians must take into account in gonorrhea treatment. METHODS: We examined the development frequency of PPNG in male patients of the venereal disease clinic of Jung-gu Community Health Center in Seoul from 1981 to 1998. Also, we analyzed the overall tendency with time series analysis using statistics package, SPSS 7.5.Grouping the years in five-year units, development frequency of PPNG of current five years (1994-1998) was compared with that of previous years. RESULTS: For the result of monthly analysis of frequency rate of PPNG development from 1981 to 1998, the rate tendency came out with a sligh rise (slope : 0.15). And for the result of comparison of grouped years, in current five years, its rate was 51.6%, marking a significant decline from that of the previous five years, which was 57.2% (p-value : <0.0001). CONCLUSION: The rate of current five years was reported to have declined from the previous years. However, the rate is still over 50%. Therefore, the government should continue the PPNG surveillance with test of sensitivity of the medications in use. Moreover, to eliminate gonorrhea, the education on high risk groups should continue and physicians should use a dose of adequate antibiotics in treatment.
Anti-Bacterial Agents ; Community Health Centers* ; Education ; Gonorrhea ; Humans ; Male ; Neisseria gonorrhoeae* ; Neisseria* ; Seoul* ; Sexually Transmitted Diseases

No abstract available.
Bronchiectasis*

No abstract available.

BACKGROUND: The number of nongonococcal urethritis(NGU) cases is exceeding more and more that of Gonorrhea in developeing nations and some of the developing countr es such as Korea. The best evidence for an etiologic role for Trichomonas Vaginalis is in nongonococcal urethritis. OBJECTIVE: We compared the effects of Tetracycline to Tetracycine and Metronidazole in the treatment of male patients with nongonococcal urethritis and wished to find out the incidence of Trichomon-as Vaginalis in male patients with nongonocaccal urethritis indirectly. METHOD: From January, ],989 to December, 1992, three hundred male patients with nongococcal urethritis(NGU) at the Venreal Disease Clinic of Choong-Ku Publi Health Center in Seoul were allocated randomly into one of two treatment regimens. and one hundred sixty eight patients were followed. RESULTS: Eighty(83.4%) of ninty six patients treated with Tetrac; cline, 500mg, PO, q.i.d. for 14 days were cured. Sixty two(86.2%) of seventy two patients treatec with Tetracycline, 500mg, PO, q.i. d. for 14 days and Metronidaole. 2.0gm, PO, for 1 day were cured. There is no significant different between the 2 groups in treated rates(P<0.05). CONCLUSION: It suggests that male patients with nongonococcal ure1hritis due to trichomoniasis are rare in Korea. This may be die to the fact that the incidence of trichorioniasis has been declining since the 1960s. It also suggests not only the need for further studies on tool is disease but also more studies on other causes of nongonocoqeal urethritis, its treatment and epidernio.ogical study.
Gonorrhea ; Humans ; Incidence ; Korea ; Male* ; Metronidazole* ; Seoul ; Tetracycline* ; Trichomonas vaginalis ; Urethritis

A 26 year old female presented complaining of a pea-sized nodule on her left lower eyelid for 10 years duration. Histopathological study showed several cysts in the dermis surrounded by secretory cells. The cysts were filled with decapitatory secretions. Positive staining with carcinoembrynic an tigen(CEA) was detected by immunoperoxidase technique. These clinical and histopathological findings suggest apocrine hidrocystoma developed on the left lower eyelid.
Adult ; Dermis ; Eyelids ; Female ; Hidrocystoma* ; Humans ; Immunoenzyme Techniques

A retrospective review of 102 patients with metastatic bone tumor seen at Hanyang University Hospital from 1986 to 1990 was performed. We analyzed the cases in the aspects of primary lesions, age and sex distributions, locations and number of metastatic foci, compared with those in the previous reports in Korea. l. 54.9% of the patients were male and 45.1% were female. The incidence of the metastatic bone tumors in female compared with the previous incidence before 1980's years was relatively increased. The peak age was in the 6th decade. The incidence younger than 50 years was decreased, and the incidence older than the 7th decade was relatively increased. 2. The main primary cancers were lung(40.2%), liver(11.8%), breast(10.8%), unknown(8.8%), and uterine cervix(6.9%) etc. In cases of lung cancer, the frequent histologic types were adenocarci noma, squamous cell carcinoma, and small cell carcinoma. 3. The vertebrae(56.9%), ribs(40.2%), femur(17.6%), pelvis(15.7%) were relatively frequent sites of metastasis which involved multiple metastasis. 4. Single metastasis(62.4%) was more frequent than multiple metastasis(37.6%). 5. In the primary cancers, the incidence in hepatoma of male was increasing, the incidence in lung cancer of female was relatively increasing, but the incidence in stomach cancer was decreasing.
Carcinoma, Hepatocellular ; Carcinoma, Small Cell ; Carcinoma, Squamous Cell ; Female ; Humans ; Incidence ; Korea ; Lung Neoplasms ; Male ; Neoplasm Metastasis ; Noma ; Retrospective Studies ; Sex Distribution ; Stomach Neoplasms

One-hundred-eleven herpes simplex viruses(HSV) were isolated from l09 patients who had visited the Department of Dermatology, Ophthalmology, and Gynecology of Hanyang University Hospital from 1986 through 1988, for suspected HSV lesions. The cultured viruses were classified into HSV-1 and HSV-2 by using direct and indirect immunofluorescent staining with monoclonal antibodies against HSV. In this study, HSV type 1 were predominantly involved in the HSV lesions above the waist of the patients(83.6%), and the infections caused by HSV type 2(I3.1 %) and bath HSV types(3.3%) were also noted. In contrast, the main organism of the HSV lesions below the waist were HSV-2(80.0%), and HSV-1(16.0% ) and both types(4.0%) were also involved in. The result suggested that the number of patients with HSV 1 genital herpes and/or with non-genital herpes by HSV-2 were apparently increasing. In conclusion, it is conceivable that the classification of HSV isolates might be useful for determining prognosis as well as performing epidemiologic studies of HSV infections.
Antibodies, Monoclonal ; Baths ; Classification ; Dermatology ; Epidemiologic Studies ; Gynecology ; Herpes Genitalis ; Herpes Simplex* ; Herpesvirus 1, Human ; Herpesvirus 2, Human ; Humans ; Ophthalmology ; Prognosis ; Simplexvirus*