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5.The effects of acute exercise on plasma concentration of follicular stimulating hormoen(FSH), estradiol, progesterone in women.

Si Young JUNG ; Hee Kyung CHOI ; Young Soo JIN ; Jae Sik SHIM ; Chang Jae LYU

Korean Journal of Obstetrics and Gynecology 1993;36(7):2843-2856

6.Comparison of Oxygenation and Lung Damage of High Frequency Flow Interruption to Conventional Ventilation in Surfactant Deficient Rabbits.

Chang Keun KIM ; Churl Young CHUNG ; Hye Jae CHO

Journal of the Korean Pediatric Society 1995;38(5):591-601

7.Restoration of Hormone Dependency in Estrogen Receptor - Lipofected MDA-MB-231 Human Breast Cancer Cells.

Young Jin SUH ; Jae Hee CHANG ; Chung Soo CHUN

Journal of the Korean Cancer Association 1999;31(3):473-482

PURPOSE: The loss of estrogen and progesterone receptors appeats to be associated with a progression to less differentiated and hormone-independent tumors. The gain of hormone independency over time even in estrogen receptor-positive tumors has become another obstacle to endocrine therapy for breast cancer. We tried to regain the hormone dependency in estrogen receptor-negative breast cancer cells by lipofecting estmgen receptor cDNA. MATERIALS AND METHODS: The mutant human estrogen receptor cDNA (pSGS-HEO) was lipofected into estrogen receptor-negative human breast cancer cell line MDA-MB-231, in an attempt to restore their sensitivity to antiestrogen. Then the effects of 17p-estradiol and tamoxifen were studied by counting viable cell numbers after treating the lipofected cell line with either one or together. RESULTS: Culture medium cantaining phenol red, a weak estrogen, has growth advantages compared with culture medium without it. In both culture conditions, cell growth was most profoundly inhibited in 4 days after lipofection with mutant human estrogen receptor cDNA, which was overcome after that day. Tamoxifen, as an antiestrogen, showed a growth inhibitory effect slightly stronger tban combined conditions of tamoxifen and 17- estradiol compared to estrogen-treated group and to control, and the inhibitory effect was lasted 4 days. CONCLUSION: The temporary induction of estrogen receptor by lipofection with pSGS-HEO on estrogen receptor-negative human breast cancer cell line MDA-MB-231 showed negative growth control on these cells by tamoxifen, indicating that liposome-mediated estrogen receptor transfection may be used as a novel therapeutic strategy for hormane independent human breast cancers in the near future.
Breast Neoplasms* ; Breast* ; Cell Count ; Cell Line ; DNA, Complementary ; Estradiol ; Estrogen Receptor Modulators ; Estrogens* ; Genetic Therapy ; Humans* ; Phenolsulfonphthalein ; Receptors, Progesterone ; Tamoxifen ; Transfection

1.Long term results and clinical evaluation of lung cancer.

2.Clinical evaluation of 32 cases aortocoronary bypass with saphenous vein.

3.Surgical treatment of pulmonary aspergillosis.

4.Double bridge PAP labelling of fibronectin in paraffin processed tissue.

5.The effects of acute exercise on plasma concentration of follicular stimulating hormoen(FSH), estradiol, progesterone in women.

6.Comparison of Oxygenation and Lung Damage of High Frequency Flow Interruption to Conventional Ventilation in Surfactant Deficient Rabbits.

7.Restoration of Hormone Dependency in Estrogen Receptor - Lipofected MDA-MB-231 Human Breast Cancer Cells.

8.Mannitol induced acute oliguric renal failure.

9.Manic patients with delusions or hallucinations.

10.Short-term outcome in manic patients with delusions or hallucinations.

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