No abstract available.
Animals ; Rats*

In the present study, immunohistochemical detection of p53 oncoprotein was performed to determine whether the grade of differentiation and the histologic type of gastric adenocarcinoma, and the degree of atypia accompanied with adenoma can be related to p53 mutation. Paraffin sections of 22 gastric adenomas and 56 gastric adenocarcinomas were examined for the overexpression of p53 oncoprotein with the avidin-biotin peroxidase complex staining procedure. The obtained results were as follows; 1. All the 22 cases of adenomas and 16 cases of well differentated adenocarcinomas showed uniformly negative staining. 2.Seven of 18 cases of moderately differentiated adenocarcinomas(39%), and five of 30 cases of poorly differentiated adenocarcinomas(17%) exhibited p53 protein expression. 3. Three of 29 cases of diffuse type (10%) and 9 of 19 cases of intestinal type(47%) exhibited p53 protein expression. These results suggest that p53 mutation is important in carcinogenesis of the intestinal type of gastric adenocarcinoma, and there is no correlation between the differentiation of gastric adenocarcinoma and the degree of p53 oncoprotein overexpression.
Adenocarcinoma ; Adenoma

No abstract available.
Neurilemmoma*

No abstract available.
Brain Infarction* ; Brain* ; Nephrotic Syndrome*

Chronic papilledema secondary to pseudotumor cerebri is potentially blinding if left untreated. We performed optic nerve sheath penetration in a 35-year-old woman with pseudotumor cerebri whose visual function worsened before optic nerve sheath penetration despite medical treatment. The diagnosis of pseudotumor cerebri was made by documenting elevated CSF pressure associated with papilledema in the department of neurology. The patient had normal CSF cytologic and chemistry studies, and normal MRI finding. The patient presented with decreased visual acuity and visual field loss despite maximum medical therapy. Before the treatment with optic nerve sheath penetration, she received acetazolamide and corticosteroids. The patient experienced sustained improvement of visual acuity and visual fields in the operated eye and the fellow unoperated eye following surgery. The patient demonstrated bilateral visual improvement after unilateral surgery. There were neither intraoperative nor postoperative complications. When surgical treatment of pseudotumor cerebri becomes necessary, we advocate optic nerve sheath decompression as a preferred treatment.
Acetazolamide ; Adrenal Cortex Hormones ; Adult ; Chemistry ; Decompression ; Diagnosis ; Female ; Humans ; Magnetic Resonance Imaging ; Neurology ; Optic Nerve* ; Papilledema ; Postoperative Complications ; Pseudotumor Cerebri* ; Visual Acuity ; Visual Fields

BACKGROUND: IL-8 is a potent chemotactic cytokine that plays an important role in the host defense mechanism against M. tuberculosis by recruiting inflammatory cells to the site of the infection. Lung epithelial cells, as well as alveolar macrophages are known to preduce IL-8 in response to M. tuberculosis. IL-8 gene expression is mainly regulated on the level of transcription by NF-κB. This study investigated whether or not A549 cells produce IL-8 in NF-κB dependent mechanism in response to macrophages phagocytosing M. tubersulosis. METHODS: Peripheral blood monocytes that were obtained from healthy donors were cultured for 24 h with M. tuberculosis and a conditioned medium(CoMTB) was obtained. As a negative control, the conditioned medium without M. tuberculosis (CoMCont) was used. A549 cells were stimulated with M. tuberculosis, CoMCont and CoMTB and the IL-8 concentration in the culture media was measured by ELISA. The CoMTB induced IL-8 mRNA expression in the A549 cells was evaluated using RT-PCR, and CoMTB induced IκBα degradation was measured using western blot analysis. CoMTB induced nuclear translocation and DNA binding of NF-κB was also examined using an electrophoretic mobility shift assay(EMSA), and the CoMTB induced NF-κB dependent IL-8 transcriptional activity was measured using a luciferase reporter gene assay. RESULTS: CoMTB induced IL-8 production by A549 cells(46.8±4.8 ng/ml) was higher than with direct stimulation with M. tuberculosis (6.8±2.9 ng/ml). CoMTB induced IL-8 mRNA expression increased after 2 h of stimulation and was sustained for 24 h. IκBα was degraded after 10 min of CoMTB stimulation and reappeared by 60 min. CoMTB stimulated the nuclear translocation and DNA binding of NF-κB. The CoMTB induced NF-κB dependent IL-8 transcriptional activity(13.6±4.3 times control) was higher than either CoMCont(2.0±0.6 times control) or M. tuberculosis (1.4±0.6 times control). CONCLUSION: A conditioned medium of peripheral blood monocytes phagocytosing M. tuberculosis stimulates NF-κB dependent IL-8 production by the lung epithelial cells.
Blotting, Western ; Culture Media ; Culture Media, Conditioned ; DNA ; Enzyme-Linked Immunosorbent Assay ; Epithelial Cells* ; Gene Expression ; Genes, Reporter ; Humans ; Interleukin-8* ; Luciferases ; Lung* ; Macrophages ; Macrophages, Alveolar ; Monocytes* ; Mycobacterium tuberculosis* ; Mycobacterium* ; RNA, Messenger ; Tissue Donors ; Tuberculosis

No abstract available.
Odontogenic Cyst, Calcifying

Animals ; Antibodies ; Capillaries ; Collagen ; Collagen Type IV ; Lasers, Gas ; Lasers, Solid-State ; Rats ; Skin ; Wound Healing ; Wounds and Injuries

No abstract available.
Lipomatosis*

No abstract available.
Arthritis, Rheumatoid* ; Arthroplasty, Replacement, Knee* ; Osteoarthritis*