1.Prostatic Intraepithelial NeoPlasia in Radical Prostatectomy Specimens as a Prognostic Factor.
Jun Hyuk HONG ; Ki Yeoul CHOI ; Tae Jin LEE ; Jae Y RO ; Choung Soo KIM
Korean Journal of Urology 2000;41(1):147-151
No abstract available.
Prostatectomy*
;
Prostatic Intraepithelial Neoplasia*
2.Prostatic Intraepithelial NeoPlasia in Radical Prostatectomy Specimens as a Prognostic Factor.
Jun Hyuk HONG ; Ki Yeoul CHOI ; Tae Jin LEE ; Jae Y RO ; Choung Soo KIM
Korean Journal of Urology 2000;41(1):147-151
No abstract available.
Prostatectomy*
;
Prostatic Intraepithelial Neoplasia*
3.Role of tyrosine kinases in vascular contraction in deoxycorticosterone acetate-salt hypertensive rats.
Cheol Ho YEUM ; Jae Yeoul JUN ; Hyo Sub CHOI
The Korean Journal of Physiology and Pharmacology 1997;1(5):547-553
It has been known that activation of tyrosine kinases is involved in signal transduction. Role of the tyrosine kinase in vascular smooth muscle contraction was examined in deoxycorticosterone acetate (DOCA)-salt hypertensive rats. Male Sprague-Dawley rats underwent uninephrectomy, one week after which they were subcutaneously implanted with DOCA (200 mg/kg) and supplied with 1% NaCl and 0.2% KCl drinking water for 4-6 weeks. Control rats were treated the same except for that no DOCA was implanted. Helical strips of carotid arteries were mounted in organ baths for measurement of isometric force development. Genistein was used as a tyrosine kinase inhibitor. Concentration-response curves to 5-hydroxytryptamine (5-HT) shifted to the right by genistein in both DOCA-salt hypertensive and control rats. Although the sensitivity to genistein was similar between the two groups, the maximum force generation by 5-HT was less inhibited by genistein in arteries from DOCA-salt hypertensive rats than in those from controls. Genistein-induced relaxations were attenuated in arteries from DOCA-salt rats. Genistein affected the contraction to phorbol 12, 13-dibutyrate (PDBu) neither in DOCA-salt nor in control arteries. These observations suggest that tyrosine kinase is involved in 5-HT-induced vascular contraction, of which role is reduced in DOCA-salt hypertension.
Animals
;
Arteries
;
Baths
;
Carotid Arteries
;
Desoxycorticosterone Acetate
;
Desoxycorticosterone*
;
Drinking Water
;
Genistein
;
Humans
;
Hypertension
;
Male
;
Muscle, Smooth, Vascular
;
Phosphotransferases*
;
Protein-Tyrosine Kinases
;
Rats*
;
Rats, Sprague-Dawley
;
Relaxation
;
Serotonin
;
Signal Transduction
;
Tyrosine*
4.Induction of Apoptosis in Synovial Cells from Patients with Rheumatoid Arthritis.
Sung Yeoul CHANG ; Seong Yoon KIM ; Myung Hee KIM ; Young Gyu CHAI ; Jae Chan PARK ; Yong Keel CHOI ; Jae Bum JUN ; Sung Soo JUNG
Korean Journal of Immunology 1997;19(4):609-616
Homeostasis of multicellular organism is controlled by proliferation and differentiation of cells as well as by cell death. The defects in programmed cell death contribute to the pathogenesis of rheumatoid arthritis (RA) and systemic lupus erythematous. RA is considered to be a proliferating disorder of synovial tissue which is accompanied by inflammatory cell infiltration and bone erosion. The aim of the study was to find whether potent inducers of apoptosis could be induced apoptosis in RA synovial cells. We examined the effects of drugs, such as dexamethasone, methotrexate, hydrogen peroxide, and ceramide on induction of apoptosis in cultured RA synovial cells. Used drugs did not induced apoptosis in RA synovial cells. Finally Fas antigen-mediated apoptosis of RA synovial cells was investigated by the addition of anti-Fas antibody. To examine the ICE (interleukin-1p-convertase; caspase-1) expression in synovial cells, RT-PCR of caspase-1 gene was performed. In synovial cells of RA, Fas induces that caspase-1 activation cause apoptosis.
Apoptosis*
;
Arthritis, Rheumatoid*
;
Cell Death
;
Dexamethasone
;
Homeostasis
;
Humans
;
Hydrogen Peroxide
;
Ice
;
Methotrexate
5.Capsaicin Inhibits the Spontaneous Pacemaker Activity in Interstitial Cells of Cajal From the Small Intestine of Mouse.
Seok CHOI ; Jae Myeong SUN ; Pawan Kumar SHAHI ; Dong Chuan ZUO ; Hyun Il KIM ; Jae Yeoul JUN
Journal of Neurogastroenterology and Motility 2010;16(3):265-273
BACKGROUND/AIMS: Capsaicin (8-methyl-N-vanillyl-6-ninenamide), a compound found in hot peppers, has been reported to have different physiological actions on different cell types. Not much work has been done about the effect of capsaicin on the function of interstitial cells of Cajal (ICC). In the present study, we examined the action of external application of capsaicin on pacemaker activity in the cultured ICC from the small intestine of mouse. METHODS: We investigated the effect of capsaicin on pacemaker currents in cultured ICC from the small intestine of mouse using a whole cell patch-clamp technique and Ca2+-imaging analysis. RESULTS: When capsaicin was applied externally to the pacemaker generating ICC, it completely inhibited the pacemaker potential under current-clamp mode (I = 0) and the pacemaker current under voltage-clamp mode at a -70 mV of holding potentials. The effect of capsaicin on pacemaker activity in ICC was shown dose dependently. The effect of capsaicin was not through the transient receptor potential of the vanilloid type 1 (TRPV1) channel as capsazepine did not block the effect of capsaicin. L-NAME, an inhibitor of nitric oxide synthase, also did not block the capsaicin-induced effects. When the action of capsaicin was examined in the intracellular calcium oscillation, it completely abolished the calcium oscillation. CONCLUSIONS: These results prove that the capsaicin has the inhibitory effects on the ICC which is carried out neither through TRPV channel nor the nitric oxide production. Intracellular Ca2+ was also an important target for actions of capsaicin on ICC.
Animals
;
Calcium Signaling
;
Capsaicin
;
Gastrointestinal Motility
;
Interstitial Cells of Cajal
;
Intestine, Small
;
Mice
;
NG-Nitroarginine Methyl Ester
;
Nitric Oxide
;
Nitric Oxide Synthase
;
Patch-Clamp Techniques
6.Involvement of Thromboxane A2 in the Modulation of Pacemaker Activity of Interstitial Cells of Cajal of Mouse Intestine.
Jin Ho KIM ; Soo Jin CHOI ; Cheol Ho YEUM ; Pyung Jin YOON ; Seok CHOI ; Jae Yeoul JUN
The Korean Journal of Physiology and Pharmacology 2008;12(1):25-30
Although many studies show that thromboxane A2 (TXA2) has the action of gastrointestinal (GI) motility using GI muscle cells and tissue, there are no reports on the effects of TXA2 on interstitial cells of Cajal (ICC) that function as pacemaker cells in GI tract. So, we studied the modulation of pacemaker activities by TXA2 in ICC with whole cell patch-clamp technique. Externally applied TXA2 (5 micrometer) produced membrane depolarization in current-clamp mode and increased tonic inward pacemaker currents in voltage-clamp mode. The tonic inward currents by TXA2 were inhibited by intracellular application of GDP-beta-S. The pretreatment of ICC with Ca2+ free solution and thapsigargin, a Ca2+-ATPase inhibitor in endoplasmic reticulum, abolished the generation of pacemaker currents and suppressed the TXA2-induced tonic inward currents. However, chelerythrine or calphostin C, protein kinase C inhibitors, did not block the TXA2-induced effects on pacemaker currents. These results suggest that TXA2 can regulate intestinal motility through the modulation of ICC pacemaker activities. This modulation of pacemaker activities by TXA2 may occur by the activation of G protein and PKC independent pathway via extra and intracellular Ca2+ modulation.
Animals
;
Benzophenanthridines
;
Endoplasmic Reticulum
;
Gastrointestinal Motility
;
Gastrointestinal Tract
;
GTP-Binding Proteins
;
Guanosine Diphosphate
;
Interstitial Cells of Cajal
;
Intestines
;
Membranes
;
Mice
;
Muscle Cells
;
Naphthalenes
;
Patch-Clamp Techniques
;
Protein Kinase C
;
Thapsigargin
;
Thionucleotides
;
Thromboxane A2
7.Altered Vascular Response to the K+induced Vasorelaxation in Aortic Smooth Muscle of Renal Hypertensive Rats.
Jae Yeoul JUN ; Cheol Ho YEUM ; Pyung Jin YOON ; Jeong Hoe LIEE ; Hyung Ho CHOI ; Yoo Whan PARK ; Jin Ho KIM
Korean Circulation Journal 2000;30(8):980-988
BACKGROUND: An increase of the extracellular K+concentrations up to about 8 mM in the isolated vessels causes relaxation in pre-contracted state. In order to elucidate the mechanisms of K+induced relaxation and compare with that of 2-kidney, 1 clip (2K1C) renal hypertensive rats, we recorded aortic vascular tension using an organ bath study. METHOD: 2K1C hypertension was made by clipping the left renal artery and age-matched control rats received a sham treatment. Thoracic aortic rings were mounted in tissue baths for measurement of isometric contractile force. RESULTS: Exposure to K+(from 2 to 8 mM) relaxed a phenylephrine (2 x 10-6 M)-induced contraction in K+free Krebs-Ringer solution, dose-dependently. Ouabain (10-5 M) enhanced the K+induced relaxation in above 2 mM K+ The K+induced relaxation was still induced in endothelium-denuded condition. Incubation with the K+channel blockers such as tetraethylammonium (TEA, 1 mM), glibenclamide (10-5 M), 4-aminopyridine (3 mM), barium (5 mM) and cesium (2 mM) did not affect on the K+induced relaxation. In renal hypertensive rats, the K+induced relaxation was markedly suppressed and ouabain enhanced it. CONCLUSIONS: These results suggest that the K+induced relaxation in aorta be mediated by Na-pump independent mechanisms, and the decrease of the K+induced relaxation in the renal hypertensive rats may be a possible mechanism of hypertension.
4-Aminopyridine
;
Animals
;
Aorta
;
Barium
;
Baths
;
Cesium
;
Glyburide
;
Hypertension
;
Muscle, Smooth*
;
Ouabain
;
Phenylephrine
;
Placebos
;
Rats*
;
Relaxation
;
Renal Artery
;
Tetraethylammonium
;
Vasodilation*
8.Altered Vascular Response to the K+induced Vasorelaxation in Aortic Smooth Muscle of Renal Hypertensive Rats.
Jae Yeoul JUN ; Cheol Ho YEUM ; Pyung Jin YOON ; Jeong Hoe LIEE ; Hyung Ho CHOI ; Yoo Whan PARK ; Jin Ho KIM
Korean Circulation Journal 2000;30(8):980-988
BACKGROUND: An increase of the extracellular K+concentrations up to about 8 mM in the isolated vessels causes relaxation in pre-contracted state. In order to elucidate the mechanisms of K+induced relaxation and compare with that of 2-kidney, 1 clip (2K1C) renal hypertensive rats, we recorded aortic vascular tension using an organ bath study. METHOD: 2K1C hypertension was made by clipping the left renal artery and age-matched control rats received a sham treatment. Thoracic aortic rings were mounted in tissue baths for measurement of isometric contractile force. RESULTS: Exposure to K+(from 2 to 8 mM) relaxed a phenylephrine (2 x 10-6 M)-induced contraction in K+free Krebs-Ringer solution, dose-dependently. Ouabain (10-5 M) enhanced the K+induced relaxation in above 2 mM K+ The K+induced relaxation was still induced in endothelium-denuded condition. Incubation with the K+channel blockers such as tetraethylammonium (TEA, 1 mM), glibenclamide (10-5 M), 4-aminopyridine (3 mM), barium (5 mM) and cesium (2 mM) did not affect on the K+induced relaxation. In renal hypertensive rats, the K+induced relaxation was markedly suppressed and ouabain enhanced it. CONCLUSIONS: These results suggest that the K+induced relaxation in aorta be mediated by Na-pump independent mechanisms, and the decrease of the K+induced relaxation in the renal hypertensive rats may be a possible mechanism of hypertension.
4-Aminopyridine
;
Animals
;
Aorta
;
Barium
;
Baths
;
Cesium
;
Glyburide
;
Hypertension
;
Muscle, Smooth*
;
Ouabain
;
Phenylephrine
;
Placebos
;
Rats*
;
Relaxation
;
Renal Artery
;
Tetraethylammonium
;
Vasodilation*
9.Cardiovascular Effects of Nifedipine and Bay K 8644 in Hypertensive Rats.
Tai Myoung CHOI ; Jong Seung KIM ; Sung Ho MOON ; Hyeong Kyun OH ; Jeong Hoe LIEE ; Jae Yeoul JUN ; Cheol Ho YEUM ; Pyung Jin YOON ; Soon Pyo HONG
Korean Circulation Journal 1997;27(12):1310-1317
BACKGROUND: Calcium plays a key role in vascular contraction and regulates receptor sensitivity to certain neurotransmitters. Calcium channel blockers are useful in the treatment of both clinical and experimental hypertension. The present study was designed to examine whether there is an alteration of the activity of calcium channels in association with the development of hypertension. METHODS: Deoxycorticosterone acetate(DOCA)-salt hypertension was made by subcutaneous implantation of DOCA(200mg/kg)strip plus saline drinking(1%) and 2-kidney, 1 clip(2KIC)hypertension by clipping the left renal artery with a silver clip(internal gap of 0.2mm). They were used 4 weeks later. Age-matched normal rats served as a control. Mean arterial pressure(MAP) and heart rate(HR) were continuously recorded from the right femoral artery. The drugs were administered intravenously. RESULTS: Vehicle alone was without effect on MAP or HR. In normotensive rats, nifedipine infusion(5 and 10ug/kg/min)caused a dose-dependent decrease in MAP without significant changes in HR, while Bay k 8644(Bay K, 5 and 10 ug/kg/min) increased MAP transiently. Both the depressor response to nifedipine and the pressor response to Bay k were more marked in DOCA-salt hypetensive rats than in normotensive rats. The maximal changes in MAP indced by nifedipine(5 and 50 ug/kg) or Bay K(5 and 50 ug/kg) were also enhanced in 2KIC hypertensive rats as compared with control rats. CONCLUSION: These results indicate that calcium channel inhibitors and activators can affect on the regulation of blood pressure in an opposite fashion. It is also suggested that the activity of calcium channels might be altered in the developement of experimental hypertension.
3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester*
;
Animals
;
Bays*
;
Blood Pressure
;
Calcium
;
Calcium Channel Blockers
;
Calcium Channels
;
Desoxycorticosterone
;
Femoral Artery
;
Heart
;
Hypertension
;
Neurotransmitter Agents
;
Nifedipine*
;
Rats*
;
Renal Artery
;
Silver
10.Transient Receptor Potential Canonical 4 and 5 Channel Antagonist ML204 Depolarized Pacemaker Potentials of Interstitial Cells of Cajal
Jun Hyung LEE ; Wen-Hao WU ; Xing-You HUANG ; Jae Yeoul JUN ; Seok CHOI
Journal of Neurogastroenterology and Motility 2020;26(4):521-528
Background/Aims:
To investigate an effect of ML204 (an inhibitor of transient receptor potential canonical 4 and 5 [TRPC4/5] channels) on interstitial cells of Cajal (ICCs) and therefore determine whether TRPC4/5 channels act on ICC-generated pacemaker activity.
Methods:
We enforced whole cell patch clamp analysis, measurements of the intracellular Ca2+ concentration, and reverse transcription polymerase chain reaction to determine the effect of ML204 (10 μM) or englerin A (a selective activator of TRPC4/5 channeles, 10 μM) and the existence of TRPC4/5 in mouse small intestinal ICC.
Results:
Treatment of ICCs with ML204 or englerin A caused the membrane potentials to depolarize. This depolarization effect of membrane potentials by ML204 in ICCs was observed to be concentration-dependent. After treating Ca 2+ - and Na + -free solutions or flufenamic acid (a non-selective cation channel blocker), the pacemaker potentials in the ICCs were abolished. A specific anoctamin 1 channel blocker did not have any effect on the pacemaker activity in ML204-untreated control cells; however, they blocked ML204-induced pacemaker activity in ICCs. Specific primers designed against TRPC4 and TRPC5 detected the presence of TRPC4/5 in small intestinal ICCs, and the application of ML204 increased raise the frequency of Ca2+ oscillations in ICCs, as assessed using Fluo-4 AM.
Conclusion
The results implied that ML204 could not inhibit the pacemaker activity but depolarized the membrane potential of ICCs by regulating intracellular Ca2+oscillations and anoctamin 1 channels.