The authors report a xase of primary non-gestational choriocarcinoma(PNGCO) of the ovary in a prepubertal female patient and reviewed. It is an extremely rere disease of which incidence is one in 369 million. Major clinical symptom is abdominal pain, precociois puberty and it can be misdiagneosed as ectopic pergnancy. Distinction from gestational choriocarcinoma(GCO) of the ovary is important because of the worse prognosis of PNGCO. But no distinctive ultrastructural or immunohistochemical differences are found between PNGCO and GCO. Most acceptable treatment modality is an aggressive surgical therapy and systemic chemotherapy, but its progrosis is poor.
Abdominal Pain ; Adolescent ; Choriocarcinoma, Non-gestational* ; Drug Therapy ; Female ; Humans ; Incidence ; Ovary* ; Prognosis ; Puberty

PURPOSE: We observed the short-term outcome and associated complications of transscleral diode laser photocoagulation (TSDL) for acute proliferative retinopathy of prematurity (ROP) to evaluate the efficacy and safety. METHODS: 20 eyes of 10 infants with acute ROP were treated with transscleral diode laser photo-coagulation through conjunctiva by a single surgeon. Anatomical results and postoperative complications were main outcomes. Follow-up ranged from 11 to 40 weeks. RESULTS: In 1 eye (5%), there was a macular temporal dragging. In 19 eyes (95%), regression occurred after a single laser treatment with a flat retina. No significant anterior or posterior segment complications were occurred, even though there was a small amount of preretinal hemorrhage in 1 eye after laser photocoagulation. CONCLUSIONS: The results suggest that transscleral diode laser photocoagulation is a safe and effective alternative to cryotherapy or transpupillary diode laser photocoagulation in the treatment of ROP.
Conjunctiva ; Cryotherapy ; Follow-Up Studies ; Hemorrhage ; Humans ; Infant ; Lasers, Semiconductor* ; Light Coagulation* ; Postoperative Complications ; Retina ; Retinopathy of Prematurity*

We report herein a case of subcutaneous granuloma annulare occured in a 3-year-old girl who had 7 subcutaneous nodules on the scalp, lumbar area, buttock, right tibial area and both ankles. liistopathologically, multiple foci of complete and incomplete collagen degeneration surrounded by histiocytes in a palisading arrangement were seen in the subcutaneous layer. Some vessel walls were thickened and infiltrated with inflammatory cells and many eosinophils were scattered among the inflamrnatory cell infiltrate around the palisaiing granuloma. Five of the lesions disappeared spontaneously and other s decreased in size without specific treatment in 2 months.
Ankle ; Buttocks ; Child, Preschool ; Collagen ; Eosinophils ; Female ; Granuloma Annulare* ; Granuloma* ; Histiocytes ; Humans ; Scalp

OBJECTIVE: The purpose of this study was to evaluate the clinical course of discordant fetal growth and perinatal outcome in relation to dichorionic and monochorionic twin for different degrees of birth weight difference. METHODS: Between Jan. 1992 and Dec, 1997, 154 twin pairs were born at Yonsei medical center and there were 36 pairs of twins which had 20% or more birth weight difference. The placental examination were performed routinely on all multiple births and 13 pairs of dichorionic twins and 23 pairs of monochorionic twins were consisted of the study population. These twin pairs were divided into three groups based on birth weight difference: 20-29%(as group A), 30-39%(as group B), and 40% or more(as group C). Gestational age, birth weight, gender and perinatal outcomes were observed for each twin pairs. RESULTS: The perinatal death in the monochorionic twin pairs showed 9(34.6%) as group A, 4(50%) as group B, and 5(41,7%) as group C and in the dichorionic twin paus 1(10.0%) as youp A, 0 as group B and 5(62.5%) as group C. Up to 40% of birth weight difference, dichorionic twin pairs showed good perinatal outcomes compared with monochorionic twin pairs but over 40% or more of birth weight diffaence, however there were no significant difference of perinatal outcomes by chorionicity. In the monochorionic twin pairs, the cause of perinatal death showed 3 cases of prematurity, 2 cases of congenital anomalies and 2 cases of IIOC and 2 cases of cord lesions in group A whenas in the group B and C 3 cases of prematurity and 6 cases of congenital anomalies. In the dichorionic twin pairs, different-sex twin pairs showed no perinatal death in all three groups but in same-sex twin pairs there were 6 perinatal deaths. CONCLUSION: More recent improved fetal ultrasonographic surveillance such as determination of chorionicity, identification of fetal sex and evaluation of fetal anomalies help to make a clinical decision in growth discordant twin up to 40% of weight difference each other.
Birth Weight ; Chorion ; Fetal Development* ; Gestational Age ; Humans ; Multiple Birth Offspring ; Pregnancy, Twin

OBJECTIVE: Our purpose was to investigate the relation between types of early diastolic notch and placental location and to evaluate the differences in perinatal outcomes. METHODS: We evaluated the Doppler waveform of the uterine artery in 149 pregnant women having an early diastolic notch after 26 weeks gestation, however we were unable to follow the obstetric outcomes in 20 women. Placental location was determined by transverse view of ultrasonography and classified as central and lateral placenta. The unilateral diastolic notches were divided into 3 groups depending on the placental location (ipsilateral: same side of the placenta, contralateral: opposite side of the placenta, and median: central placenta) RESULTS: In the bilateral notch group, the S/D ratio of the contralateral uterine artery was higher than the ipsilateral one(n=60, mean=3.22 Vs 2.80, p=0.0067). Of the 89 unilateral notches observed, the S/D ratio of the uterine artery in patients having early diastolic notch was higher compared to those without early diastolic notch.(t-test, mean=2.74,1.92, p=0.0001) Patients with bilateral notches were associated with poor perinatal outcomes significantly more than the patients with unilateral notches(p=0.003). Among the unilateral groups (ipsilateral: n=10, median: n=21, contralateral: n=58), there was no significant difference in perinatal outcomes. CONCLUSION: The early diastolic notch can be observed on both or either sides of the uterine artery independent on placental location. More bilateral notches were associated with poor pregnancy outcomes than unilateral notches. The pregnancy outcomes tended to be similar in unilateral notch irrespective of placental location, but showed worse outcomes than the control group. Careful analysis of the uterine artery in nonplacental side is necessary because of the frequent appearance of early diastolic notch and higher vascular resistance.
Female ; Humans ; Placenta ; Pregnancy ; Pregnancy Outcome ; Pregnant Women ; Rheology* ; Ultrasonography ; Uterine Artery* ; Vascular Resistance

Twin reversed arterial perfusion (TRAP) sequence is a rare specific anomaly of twin gestation with fused placenta and umbilical anastomosis. This syndrome occurs once in about 34,600 births and reported first by Beneditti in 1533. We report on prenatal diagnosis of a case of TRAP sequence with color Doppler sonography, this case is presented with a brief review of the literature.
Humans ; Parturition ; Perfusion ; Placenta ; Pregnancy ; Prenatal Diagnosis*

Aniridia is a relatively rare congenital anomaly and its incidence is about 1:100, 000.Main features of aniridia include congenitally partial or nearly complete absence of the iris and hypoplasia of optic disc and fovea. It is almost bilateral and occurred as an autosomal dominant trait.However, some patients develop both sporadic nonfamilial aniridia and Wilms 'tumor, and another group of patients is transmitted as an autosomal recessive trait. We observed aniridia in two generations, as mother and two daughters. They had cataract, nystagmus, neovascularization of corneal margin and pannus formation, as well as aniridia.We also found hypoplasia of optic disc and fovea in mother. We have operated on mother with phacoemulsified lens aspiration and posterior chamber IOL implantation in both eyes consecutively.The postoperative visual acuity following lens extraction improved, but was not corrected. In addition, intraocular pressure decreased.
Aniridia* ; Cataract ; Family Characteristics ; Humans ; Incidence ; Intraocular Pressure ; Iris ; Mothers ; Nuclear Family ; Visual Acuity

No abstract available.
Diabetes Mellitus, Type 1* ; Echocardiography*

Interleukin-6 (IL-6) is introduced as a marker of disease. At present, a variety of method may be used to quantify expression of this protein. Antigen capture-ELISA is a sensitive and accurate quantification method previously used with ovine, rat, and human IL-6 proteins. However, it has never been reported to quantify porcine IL-6 protein using capture ELISA. In this study, we generated and characterized a set of IgY and mono-specific polyclonal antibodies to recombinant porcine IL-6 (rpIL-6), and combining these with a sensitive and specific capture-ELISA for a diagnostic purpose. cDNA encoding the mature protein coding region of porcine IL-6 was cloned and expressed with pQE-30UA expression vector. rpIL-6 was then expressed and purified by using Ni-NTA resin. Protein mass of 24 kDa was found with SDS-PAGE and the identity of the protein was confirmed by Western-blot. Production of polyclonal antibodies against rpIL-6 was performed using the purified rpIL-6 in mice and hens. An antigen capture-ELISA was developed with the antibodies after their extraction. To compare the IL-6 level in the different sanitary state of farms, pig sera were randomly collected and concentration of IL-6 in the sera was measured with the antigen capture-ELISA. The capture-ELISA with the optimal concentration of antibodies, in this study, was able to detect about 10 ng/ml of rpIL-6. IL-6 levels determined with the capture-ELISA in pig sera showed positive correlation with the sanitary states of the farms. These results suggested that the developed antigen capture-ELISA could be a good tool for the screening of microbial infection in pig farms.
Animals ; Biological Markers/blood ; Blotting, Western/veterinary ; Chickens ; Cloning, Molecular ; DNA, Complementary/genetics/isolation&purification ; Electrophoresis, Polyacrylamide Gel/veterinary ; Enzyme-Linked Immunosorbent Assay/methods/*veterinary ; Female ; Immunoglobulins/*blood ; Interleukin-6/*immunology ; Mice ; Mice, Inbred ICR ; Recombinant Proteins/immunology ; Swine/*immunology

No abstract available.
Nuchal Cord* ; Ultrasonography*