Infantile hypertrophic pyloric stenosis (IHPS) is the most common condition requiring abdominal surgery in early infancy, and is caused by hypertrophied pyloric muscle. The development of successful surgical treatment in the early 1900s by Fredet and Ramstedt made it possible for infants worldwide to survive. Modern pediatric anesthetic techniques have virtually eliminated mortality from surgical management. Atropine sulfate is a cholinergic blocking agent with potent antimuscarinic activity that decreases peristaltic contractions by relaxing smooth muscles. We treated two cases of IHPS with incomplete pyloromyotomy in 3-month-old and 5-month-old male infants by administering atropine sulfate intravenously. They were free from vomiting after 5 days of intravenous atropine sulfate treatment. In these rare cases of persistent vomiting or refractory emesis following incomplete pyloromyotomy, there may be a role for atropine sulfate.
Atropine* ; Constriction, Pathologic* ; Humans ; Infant ; Male ; Mortality ; Muscle, Smooth ; Pyloric Stenosis, Hypertrophic ; Vomiting*

No abstract available.
Alcoholics* ; Humans ; Skin*

This study was conducted to observe immunological abnormalities, vscular changes and abnormal maturation, pathway of keratinocyte in the psoriasis, by comparing drposits of IgG, fibrinogen(FG) and fibronectin(FN) in the invnlved and uninvolved skin of 19 psoriaics before treatment, the involved skin of 12 among the above patients after treatment, and the skin of 8 normal controls. The study was carried out by using direct immunofluorescence technique with anti-IpG, anti-FG, anti-FN antibodies. The results are summarized as follows: 1. In the uninvolved skin of 19 psoriatics before treatment, no depasition of IgG was observed while deposition of FG was present at the dermo-epidermal junction in 5.3% of the patients and dermis(10.5 %). Deposition of FN was found at the dermo-epidermal junction(52%), papillary dermis(57.9%) and dermal blood vessels(52.6%). 2. In the involved skin of 19 psoriatics before treatment, deposition of IgG was observed in the horny layer in 84.2% of the patients, upper epidermis(15.8%) and depositicin of FG was present in the horny layer(52.6%), upper epidermi(63.1%), dermo-epidermal junction(15.8%), upper dermis(15.8%) and dermal blood vessels(5.3%). Leposition of FN was found in the horny ayer(26.3%), upper epidermis (36.8%), dermo-epidermal junction(78.9%), upper dermis(89.5%) and ermal blood vessels(68.4%). 3. In the involved skin of 12 psoriatics after treatment, IgG was wealily deposited in the horny layer in 16.7% of the patients and deposition of FG was observed in the horny layer(8.3%), upper epidermis (16.7%), dermo-epidermal junction(8.3%) and dermis(8.3%). Deposition of FN was found in the upper epiderrnis(8.3%), dermo-epiderrnal junction(58.3%), dermis(66.%) and dermal blood vessels (33.3%) 4. In the normal skin of 8 control subjects, no deposition of IgG and, FG was observed while deposition of FN was found at the inummo-epidermal junction in 37.5% of the persons, upper dermis(25%) and dermal blood vessels(12.5%)
Antibodies ; Blood Vessels ; Epidermis ; Fibrinogen ; Fibronectins* ; Fluorescent Antibody Technique, Direct ; Humans ; Immunoglobulin G ; Immunoglobulins ; Keratinocytes ; Psoriasis ; Skin

No abstract available.
Epidemiologic Studies* ; Female ; Pregnancy ; Pregnancy, Ectopic*

No abstract available.
Drug Therapy, Combination* ; Ulcer*

No abstract available.
Chinchilla* ; Neurons*

Silicone gel sheeting is widely used to manage the hypertrophic or keloid scars. Since first reported in 1982 to be an effective treatment for burn scars and contractures, many authors reported its efficacy to treat scars. Chemically silicone gel sheet composed of cross-linked dimethy1 and vinyl enblocked polydimethylsiloxane polymer. The exact mechanism of silicone gel sheet to treat hypertrophic scar was still unknown, but decreasing the water vapor transmission was supposed to level the scar. During out clinical experience, a few patients suffered from skin problems by silicone gel sheeting. So we designed a study to determine the severity of skin hypersensitivity of silicone gel sheeting. Four types of silicone gel sheets were applied to upper arms of 140 healthy voluntees. Resultant skin lesions were analysed 48 and 96 hours later to differentiate the irritation and the true hypersensitivity. About 30 percent of voluteers represented mild skin irritability(48 hours later), but true skin hypersensitivity was not found(96 hours later). The site to be applied with silicone gel sheet is very critical area, so pretesting the irritability of silicone gel sheeting to individuals is an important step to control the hypertrophic scar.
Arm ; Burns ; Cicatrix ; Cicatrix, Hypertrophic ; Contracture ; Humans ; Hypersensitivity* ; Keloid ; Patch Tests* ; Polymers ; Silicone Gels* ; Skin* ; Steam

No abstract available.
Melanoma* ; Proliferating Cell Nuclear Antigen*

Tri- and tetra-cyclic antidepressants are known to cause dry mouth among other several major complications. The present study was designed to compare the degree of reduced salivation due to antidepressants and to explore whether intracellular calcium-increasing agents ameliorate the salivation. Effects of antidepressants and agents increasing intracellular calcium on the cholinergic submandibular secretion and blood flow induced by the chorda stimulation or intra-arterial acetylcholine were observed in anesthetized cats. Effects of antidepressants and calcium-mobilizing agents on K+ efflux were also observed in excised gland slices. The results obtained were as follows: 1. Salivary secretion in response to the chorda stimulation (3 V, 20 Hz, 1 msec) was significantly attenuated by antidepressants in a dose-dependent manner, whereas the blood flow was not affected. 2. Salivary secretion and increased blood flow evoked by intra-arterial acetylcholine (20 microgram/kg) were markedly diminished by antidepressants, the magnitude of which was amitryptyline>imipramine >mianserin in order. 3. Cholinergic salivation was significantly decrease by cyclopiazonic acid, a calcium pump inhibitor of the endoplasmic reticulum, or by BAPTA/AM, a specific intracellular calcium chelator. 4. Caffeine and ryanodine potentiated the cholinergic salivation and ameliorated the depressed salivary secreation due to antidepressants. 5. Calcium ionophore A 23187 ameliorated the depressed salivation due to antidepressants. 6. Antidepressants inhibited the K+ efflux, which were restored by caffeine or A 23187. These results suggest that the depressed salivary secreation due to antidepressants is ameliorated by increasing intracellular calcium levels.
Acetylcholine ; Animals ; Antidepressive Agents ; Caffeine ; Calcimycin ; Calcium* ; Cats ; Endoplasmic Reticulum ; Mouth ; Ryanodine ; Salivation*

Thelper (Th) cells play a pivotal role in the regulation of immune responses. Since Th epitopes in adenovirus have not yet been defined, in this study, it was attempted to search for Th epitopes of adenovirus antigens that are restricted by MHC class II (H-2E). Among candidate viral proteins to be screened for Th epitopes, structural protein was selected, since they induced strong IL-2 release from adenovirus immune lyrnph node (LN) cells and the presence of E1 protein, which contains immunodominant cytotoxic T lymphocyte epitopes, did not potentiate the T cell responses. To confirm the presence of Th epitopes in the structural protein, virions were trypsinized and the resulting polypeptides whose molecular weights were lower than 5,000 were fractionated by HPLC. Some of the HPLC fraction turned out to induce LN cell proliferation. Ten synthetic peptides were designed as candidate Th epitopes from the primary amino acid sequences of adenovirus hexon and penton protein which are major constituents of the virion. The selected sequences share the common features of other known H-2E' binding ligands. Among these ten synthetic peptides, peptide of hexon protein amino acid residue 709-721 induced noticeable proliferation of LN cells from preimmune mice, and also able to induce IL-2 secretion from adenovirus-specific T hybridomas, suggesting that the peptide was the most immunodominant Th epitope. Hexon protein 221-233 and hexon protein 676-688 are considered as epitopes also. This study revealed three epitope sequences from adenovirus structural protein that are presented by class II MHC, H-2E.
Adenoviridae* ; Amino Acid Sequence ; Animals ; Cell Proliferation ; Chromatography, High Pressure Liquid ; Epitopes ; Epitopes, T-Lymphocyte* ; Hybridomas ; Interleukin-2 ; Ligands ; Mice ; Molecular Weight ; Peptides ; Trypsin ; Viral Proteins ; Virion