1.Mutation of the Chk1 Gene in Gastric Cancers with Microsatellite Instability.
Jong Heun LEE ; Young Gu CHO ; Jae Whie SONG ; Cho Hyun PARK ; Suk Woo NAM ; Sug Hyung LEE ; Nam Jin YOO ; Jung Young LEE ; Won Sang PARK
Journal of the Korean Gastric Cancer Association 2005;5(4):260-265
PURPOSE: The protein kinase Chk1 is required for cell cycle arrest in response to DNA damage and is shown to play an important role in the G2/M checkpoint. The aim of this study was to investigate the relationship between microsatellite instability and frameshift mutation of the Chk1 gene in gastric cancers. MATERIALS AND METHODS: The microsatellite instability was analyzed in 95 primary gastric carcinomas by using microdissection and 6 microsatellite markers. We also performed single strand conformational polymorphism and sequencing to detect frameshift mutation of the Chk1 gene. RESULTS: We found positive microsatellite instability in 19 (20%) of the 95 gastric cancers, 13 high- and 6 low-frequency microsatellite instability cases. The frameshift mutation of Chk1, which resulted in a truncated Chk1 protein, was detected in two high-frequency microsatellite instability cases. CONCLUSION: These data suggest that the microsatellite instability may contribute to the development of gastric carcinomas through inactivation of Chk1.
Cell Cycle
;
Cell Cycle Checkpoints
;
DNA Damage
;
Frameshift Mutation
;
Microdissection
;
Microsatellite Instability*
;
Microsatellite Repeats*
;
Protein Kinases
;
Stomach Neoplasms*