Vibrio fluvialis is one of the non-cholera, halophilic, gram-negative bacterium that causes gastroenteritis. There have been a few reports of V. fluvialis infections worldwide. In the present report, one case of gastroenteritis caused by V. fluvialis which occurred in chronic liver disease patient in Korea is presented. This was a rare case of gastroenteritis caused by V. fluvialis in 39-years old man who had no history of seafood ingestion. He was admitted because of severe watery diarrhea and abdominal pain, and developed acute renal failure. The patient was improved after fluid and antibiotic therapy for 2 weeks.
Abdominal Pain ; Acute Kidney Injury ; Adult ; Diarrhea ; Eating ; Gastroenteritis* ; Humans ; Korea ; Liver Diseases ; Seafood ; Vibrio*

Vibrio fluvialis is one of the non-cholera, halophilic, gram-negative bacterium that causes gastroenteritis. There have been a few reports of V. fluvialis infections worldwide. In the present report, one case of gastroenteritis caused by V. fluvialis which occurred in chronic liver disease patient in Korea is presented. This was a rare case of gastroenteritis caused by V. fluvialis in 39-years old man who had no history of seafood ingestion. He was admitted because of severe watery diarrhea and abdominal pain, and developed acute renal failure. The patient was improved after fluid and antibiotic therapy for 2 weeks.
Abdominal Pain ; Acute Kidney Injury ; Adult ; Diarrhea ; Eating ; Gastroenteritis* ; Humans ; Korea ; Liver Diseases ; Seafood ; Vibrio*

The interstitial cells of Cajal (ICCs) are the pacemaker cells in gastrointestinal tract and generate electrical rhythmicity in gastrointestinal muscles. Therefore, ICC may be modulated by endogenous agents such as neurotransmitter, hormones, and prostaglandins (PGs). In the present study, we investigated the effects of prostaglandins, especially PGE2, on pacemaker currents in cultured ICCs from murine small intestine by using whole-cell patch clamp techniques. ICCs generated spontaneous slow waves under voltage-clamp conditions and showed a mean amplitude of -452+/-39 pA and frequency of 18+/-2 cycles/min (n=6). Treatments of the cells with PGE2 (1muM) decreased both the frequency and amplitude of the pacemaker currents and increased the resting currents in the outward direction. PGE2 had only inhibitory effects on pacemaker currents and this inhibitory effect was dose-dependent. For characterization of specific membrane EP receptor subtypes, involved in the effects of PGE2 on pacemaker currents in ICCs, EP receptor agonists were used: Butaprost (1muM), EP2 receptor agonist, reduced the spontaneous inward current frequency and amplitude in cultured ICCs (n=5). However sulprostone (1muM), a mixed EP1 and EP3 agonist, had no effects on the frequency, amplitude and resting currents of pacemaker currents (n=5). SQ-22536 (an inhibitor of adenylate cyclase; 100muM) and ODQ (an inhibitor of guanylate cyclase; 100muM) had no effects on PGE2 actions of pacemaker currents. These observations indicate that PGE2 alter directly the pacemaker currents in ICCs, and that the PGE2 receptor subtypes involved are the EP2 receptor, independent of cyclic AMP- and GMP-dependent pathway.
Adenylyl Cyclases ; Dinoprostone* ; Gastrointestinal Tract ; Guanylate Cyclase ; Interstitial Cells of Cajal* ; Intestine, Small* ; Membranes ; Muscles ; Neurotransmitter Agents ; Patch-Clamp Techniques ; Periodicity ; Prostaglandins