1.Transplantation of Cultured Keratinocytes in Autologous Fibrin Glue Suspension.
Jin Young KIM ; Sung Pyo HONG ; Jae Kyung PARK
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1999;26(4):531-537
The use of a cultured autologous keratinocyte sheet has become a recognized method for the coverage of extensive bums during recent years. The disadvantages of these sheet grafts are a long time-lag until keratinocyte sheets are available, the fragility and difficulty in handling of grafts, an unpredictable take rate and extremely high costs. In this study we investigated the transplantation of cultured keratinocytes as single cells suspended in autologous fibrin glue. In a rat model with standardized full thickness wounds, this new transplantation technique was evaluated and compared directly to the conventional keratinocyte sheet grafting technique. After transplantation, wounds were evaluated for the degree of epithelial coverage, and then microscopic structures were evaluated under light and electron microscopy. The results were as follows: 1) The fibrinogen solution prepared from autologous blood had 12 times more fibrinogen compared to the original blood. 2) After transplantation of cultured keratinocyt-es in fibrin glue, the degree of epithelial coverage was 79% at 2 weeks, which was comparable to 17% for cultured keratinocyte sheet graft 3) Typical basement membrane structures were consistently found at 2 weeks after transplantation of keratinocytes in fibrin glue. 4) Rete ridges were found at 4 weeks after transplantation of keratinocytes in fibrin glue. In conclusion, the transplantation technique of keratinocytes in fibrin glue is available earlier than sheet grafts, it transfers actively proliferating cells and it simplifies the grafting procedure. As well, this technique leads to an earlier epithelial covering and an earlier restoration of the dermo-epidermal junction than sheet grafting.
Basement Membrane
;
Fibrin Tissue Adhesive*
;
Fibrin*
;
Fibrinogen
;
Keratinocytes*
;
Microscopy, Electron
;
Models, Animal
;
Transplants
;
Wounds and Injuries
2.A study on the preparation of an acellular allogenic dermis and usefulness in a full thickness wound model.
Sung Pyo HONG ; Se Kwang OH ; Jae Kyung PARK ; Doo Hyung LEE
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1998;25(5):740-748
The use of artificial skins for full thickness wounds is an accepted technique, but unfortunately the take rate is low and the aesthetical result is not acceptable. The freeze-drying treatment of allogenic tissues can destroy cells with preserving the structural organization of extracellular matrices, permitting allogenic transplantation. In this study we investigated a new method to process the allogenic skin for transplantable allogenic dermis and this dermis was evaluated in a full thickness wound model. The results are as followings; 1. After treatment with NaCl and SDS solution and then with freeze-drying method, the allogenic dermis shows acellular dermal matrix with preserved normal extracellular matrix. 2. This allogenic dermis became completely incorporated into the wound without evidence of rejection or replacement by scar tissue. 3. The take rate of thin autografts overlying the allogenic dermis that were applied simultaneously was comparable to take rate of autograft alone. 4. The reduction in secondary contraction by allogenic dermis treated wounds was significant. 5. After grafting with cultured keratinocytes, the degree of epithelial coverage was 70% at 2 weeks. In conclusion, the allogenic dermis processed with our method displayed lack of antigenicity, and rapid revascularization. This allogenic dermis can permit simultaneous engraftment of an overlying STSG or cultured kerationocytes, reduce secondary contraction and improve cosmesis of full thickness wounds.
Acellular Dermis
;
Autografts
;
Cicatrix
;
Dermis*
;
Extracellular Matrix
;
Keratinocytes
;
Skin
;
Skin, Artificial
;
Transplants
;
Wounds and Injuries*
3.A study on the applicability of polymer(polyglactin)for the artificial dermis.
Sung Pyo HONG ; Jae Kyung PARK ; Seung Ho HUH ; Hong Seok KIM
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1998;25(4):547-556
The result of artificial skins made with collagen is poor after grafting over the full thickness wounds due to their rapid degradation by enzymatic cleavage. This study is an in vivo study of an artificial skin made with a biodegradable polymer, which can better address the problem of the collagenous artificial dermis. To investigate the availability of a biodegradable polymer for an artificial dermis and to get an information about the optimal degradation rate of a polymer for an artificial dermis, we made an artificial dermis by seeding of fibroblasts within the vicryl mesh and made a bilayer artificial skin by covering the artificial dermis with cultured keratinocytes. And these artificial dermis and artificial skin were evaluated in a full thickness wound model. The results are as followings: 1. The artificial dermis was available for grafting for 1 week culture of vicryl mesh-fibroblast. 2. The artificial dermis retarded the contraction of full thickness wounds. 3. The artificial dermis generated the granulation tissue and accepted the STSG completely. 4. The generated tissue from the artificial dermis had incorporated into the surrounding tissue by 4 weeks postgrafting. 5. Vicryl in the artificial dermis became to biodegrade from the culture period and absorbed completely by 5 weeks. 6. The epidermal portion was poorly differntiated during in vitro culture period. In conclusion, the polymer-fibroblast graft can retard the wound contraction and generate a new tissue permitting a useful dermal replacement. And to get more optimal results, another polymer which has slower biodegradation rate than vicryl should be used for the artificial dermis and the epidermal portion should be differentiated after in vivo grafting.
Collagen
;
Dermis*
;
Fibroblasts
;
Granulation Tissue
;
Keratinocytes
;
Polyglactin 910
;
Polymers
;
Skin, Artificial
;
Transplants
;
Wounds and Injuries
4.In Vitro Tissue Engineering of Cartilage using Autologous Fibrin Glue and Chondrocytes.
Sung Pyo HONG ; Jin Sik BURM ; Jae Kyung PARK ; Jin Young KIM ; Doo Hyung LEE
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1999;26(3):383-391
Neomorphogenesis of cartilage using chondrocyte-polymer constructs is a potential source for development of cartilage reconstruction. Current tissue engineering techniques of neocartilage rely on in vivo implantation of polymer-chondrocyte constructs. The purpose of this study was to find a way to bioengineer cartilage in vitro by entrapping chondrocytes in a molded autologous fibrin glue. Chondrocytes isolated from the cartilage of rabbit joints were combined with fibrinogen extracted by a single cryoprecipitation of autologous plasma, and they were then polymerized with thrombin to create a fibrin glue with a final cell density of 2.5x10(6) cells/ml. The collagen for a control study was used as a polymer. The polymer-chondrocyte constructs were cultured for 4 weeks and the fibrin-chondrocyte constructs molded in the shape of a human ear were cultured for 6 weeks in vitro. Morphometric, histochemical, and histomorphometric analysis including glycosaminoglycan quantitation confirmed the following results: 1) Highly-concentrated autologous fibrinogen was easily extracted by a single cryoprecipition of autologous olasma. 2) The fibrin-chondrocyte constructs demonstrated the presence of actively proliferating chondrocytes with the production of cartilaginous matrix(collagen and glycosaminoglycan) at 1 week after culture, as well as gross and histologic evidence similar to those of normal cartilage at 3-4 weeks after culture. 3) The collagen-chondrocyte constructs demonstrated lower degrees of hardness and transparency, as well as a lower density of cells and glycosaminoglycan during the culture period. 4) Neocartilage generated from fibrin-chondrocyte constructs in the shape of a human ear nearly retained their original configuration and size without degeneration for 6 weeks of culture in vitro. This study demonstrated a novel method for bioengineering the molded cartilage in vitro using autologous fibrin glue as a matrix scaffold. The generated cartilage showed gross and histologic evidence similar to those of normal cartilage, retaining the original gross dimension. With further refinement, this may be a new application of tissue engineering for the reconstruction of cartilage.
Bioengineering
;
Cartilage*
;
Cell Count
;
Chondrocytes*
;
Collagen
;
Ear
;
Fibrin Tissue Adhesive*
;
Fibrin*
;
Fibrinogen
;
Fungi
;
Hardness
;
Humans
;
Joints
;
Plasma
;
Polymers
;
Thrombin
;
Tissue Engineering*
5.A STUDY ON THE DEVELOPMENT OF BIOSYNTHETIC SKIN REPLACEMENTS CONSISTED OF FIBROBLASTS GROWN IN BIOBRANE.
Hong Seok KIM ; Sung Pyo HONG ; Jae Kyung PARK ; Doo Hyung LEE
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1997;24(5):875-883
No abstract available.
Fibroblasts*
;
Skin*
6.A Case of Esophageal Ulcerations in Behcet's Disease.
In Suh PARK ; Jae Bock CHUNG ; Ho Guen KIM ; Sung Pyo HONG ; Dong Sik BANG
Korean Journal of Gastrointestinal Endoscopy 1994;14(4):425-429
Trminal ileum and cecum are the most frequently involved portions of the gastrointestinal tract in Behqet's disease. Esophageal involvement in Behqet's disease is very uncommon and only a few cases have previously been reported. We have observed a case with this disease. A 25-year-old female patient who was diagnosed as having Behqet's disease 7 years before had dysphagia and weight loss of about 10 kg for 4 months. Esophagogastroduodenoscopic examination revealed diffuse ulceration and mucosal nodularities in esophagus which suspected esophageal cancer. But repeated biopsies showed lymphocytic infiltration around vessels in ulcerative lesions. She was treated with conservative managements.
Adult
;
Biopsy
;
Cecum
;
Deglutition Disorders
;
Esophageal Neoplasms
;
Esophagus
;
Female
;
Gastrointestinal Tract
;
Humans
;
Ileum
;
Ulcer*
;
Weight Loss
7.A Case of Primary Malignant Melanoma of the Vagina: Vulvovaginal Reconstruction Using Gracilis My ocutaneous Flap Afer Radical Surgery.
Jae Don JUNG ; Chang Nam KIM ; Jong Woo SOHN ; Sung Pyo HONG ; Seon Kyung LEE
Korean Journal of Gynecologic Oncology and Colposcopy 1995;6(1):45-51
Primary malignant melanornn of the vagina is rare, with only about 130 reported cases worldwide. They presumably arise from melanocytes that are present in the vagina in 3% of normal wornen. Vaginal melanoma accounts for less than 1% of melanomas and less than 3% of primary malignant tumors of the vagina. Most of patients complain vaginal bleeding, vaginal discharge, foreign body sensation in order. The best treatment of vaginal melanoma rernains eni.gmatic, Many authors indicate that radical surgery may be the pcferred approach and the number of reported cases treated with radical surgery is increasing. But other author comment that no significant change in survival is apparent with radical surgery. Overall prognosis is poor as most patients have deeply penetrating lesions at the time of diagnosis. Recently, not only the effort to increase the survival rate but the quality of life including sexual function after treatment is becoming an important issue about treatment ot cancer patients. We experienced a case of primary malignant melanoma of the vagina treated with radical surgery and restored the sexual function by vulvovaginal reconstruction using gracilis myocutaneous flap. So we report this case with brief literature review.
Diagnosis
;
Foreign Bodies
;
Humans
;
Melanocytes
;
Melanoma*
;
Myocutaneous Flap
;
Prognosis
;
Quality of Life
;
Sensation
;
Survival Rate
;
Uterine Hemorrhage
;
Vagina*
;
Vaginal Discharge
8.Drug resistance of mycobacterium tuberculosis in Korea.
Sang Jae KIM ; Young Pyo HONG ; Yong Chul HAN ; Sung Jin KIM
Tuberculosis and Respiratory Diseases 1991;38(2):99-107
No abstract available.
Drug Resistance*
;
Korea*
;
Mycobacterium tuberculosis*
;
Mycobacterium*
9.Immunohistochemical Study of p53 Gene Protein and bcl-2 Gene Protein Expression in Gastric Adenocarcinoma.
Jae Pyo YANG ; Sung Jae CHA ; Sung Joon PARK ; Hyun Muc LIM ; Sung Il PARK ; Un Seop PARK
Journal of the Korean Surgical Society 1998;54(3):353-362
The p53 oncoprotein, a product of the tumor suppressor gene encoded on the short arm of chromosome 17, has been noted in a number of human tumors as a tumor suppressor and some what has been related with cellular apoptosis. Thus, mutant p53 inhibits apoptosis. Like the mutant p53 oncoprotein, the bcl-2 oncoprotein expressed in various epithelial and nonepithelial cells plays a major role in inhibiting cellular apoptosis. To elucidate the role of bcl-2 and mutant p53 oncoprotein expression in gastric adenocarcinomas, immunohistochemical stains were carried out in 60 cases of gastric adenocarcinomas including 10 cases of early gastric cancer. We studied the expression patterns of the bcl-2 and the mutant p53 protein according to age, sex, histologic differentiation, tumor location, tumor size, lymph-node involvement, and depth of tumor invasion. The results were as follows: 1) p53 protein expression was detected in 39 of 60 cases (65%), and bcl-2 protein expression was detected in 29 of 60 cases (48%). 2) The p53 and the bcl-2 expression rates for early gastric cancer were 60% and 50%, and those for advanced gastric cancer were 66% and 40%, respectively. 3) There was no significant correlation of p53 or bcl-2 expression with sex, age, histologic differentiation, tumor location, tumor size, and lymph-node involvement; however, the expression of p53 was correlated with the depth of tumor invasion (p=0.049). Based on the present study, the expression of p53 is thought to be correlated with tumor progression and may be a useful prognostic factor in gastric adenocarcinomas.
Adenocarcinoma*
;
Apoptosis
;
Arm
;
Chromosomes, Human, Pair 17
;
Coloring Agents
;
Genes, bcl-2*
;
Genes, p53*
;
Genes, Tumor Suppressor
;
Humans
;
Immunohistochemistry
;
Stomach Neoplasms
10.The Changes of Aqueous Vasopermeability Factors After Intravitreal Triamcinolone Injection for Branch Retinal Vein Occlusion.
Seung SONG ; Sung Pyo PARK ; Jae Kyoun AHN
Journal of the Korean Ophthalmological Society 2008;49(11):1765-1770
PURPOSE: To investigate the changes of aqueous vascular endothelial growth factor (VEGF) and interleukin (IL)-6 in patients with acute macular edema secondary to recent-onset branch retinal vein occlusion (BRVO) after a single intravitreal injection of triamcinolone acetonide (IVTA) METHODS: Aqueous and plasma levels of VEGF and IL-6 were measured by ELISA in ten controls and thirty patients at the time of IVTA and 3 months afterward. We compared the aqueous levels of VEGF and IL-6 and the clinical course between responders and non-responders. RESULTS: The aqueous levels of VEGF and IL-6 were significantly higher in non-responders than in responders at baseline measurements (495+/-259 pg/ml vs. 223+/-110 pg/ml, P<.001; 36+/-32 pg/ml vs. 16+/-19 pg/ml, P=.037, respectively). The aqueous levels of VEGF were still higher in non-responders (303+/-75 pg/ml) 3 months after IVTA, while the aqueous levels of VEGF in responders returned to normal (77+/-23 pg/ml, P<.001). The aqueous levels of IL-6 normalized in all patients 3 months after IVTA. In non-responders, central foveal thickness was significantly higher, and foveal ischemia and a wide non-perfused area were more common. CONCLUSIONS: Non-IL6-dependent VEGF may contribute to persistent or recurrent ischemic macular edema associated with BRVO after IVTA.
Enzyme-Linked Immunosorbent Assay
;
Humans
;
Interleukin-6
;
Interleukins
;
Intravitreal Injections
;
Ischemia
;
Macular Edema
;
Plasma
;
Retinal Vein
;
Retinal Vein Occlusion
;
Retinaldehyde
;
Triamcinolone
;
Triamcinolone Acetonide
;
Vascular Endothelial Growth Factor A