No abstract available.
Carcinoma, Squamous Cell* ; Cervix Uteri* ; Female ; Proliferating Cell Nuclear Antigen*

No abstract available.
Carcinoma, Squamous Cell* ; Cervix Uteri* ; Female ; Nucleolus Organizer Region*

No abstract available.
Thyroid Gland* ; Thyroid Nodule*

Tri- and tetra-cyclic antidepressants are known to cause dry mouth among other several major complications. The present study was designed to compare the degree of reduced salivation due to antidepressants and to explore whether intracellular calcium-increasing agents ameliorate the salivation. Effects of antidepressants and agents increasing intracellular calcium on the cholinergic submandibular secretion and blood flow induced by the chorda stimulation or intra-arterial acetylcholine were observed in anesthetized cats. Effects of antidepressants and calcium-mobilizing agents on K+ efflux were also observed in excised gland slices. The results obtained were as follows: 1. Salivary secretion in response to the chorda stimulation (3 V, 20 Hz, 1 msec) was significantly attenuated by antidepressants in a dose-dependent manner, whereas the blood flow was not affected. 2. Salivary secretion and increased blood flow evoked by intra-arterial acetylcholine (20 microgram/kg) were markedly diminished by antidepressants, the magnitude of which was amitryptyline>imipramine >mianserin in order. 3. Cholinergic salivation was significantly decrease by cyclopiazonic acid, a calcium pump inhibitor of the endoplasmic reticulum, or by BAPTA/AM, a specific intracellular calcium chelator. 4. Caffeine and ryanodine potentiated the cholinergic salivation and ameliorated the depressed salivary secreation due to antidepressants. 5. Calcium ionophore A 23187 ameliorated the depressed salivation due to antidepressants. 6. Antidepressants inhibited the K+ efflux, which were restored by caffeine or A 23187. These results suggest that the depressed salivary secreation due to antidepressants is ameliorated by increasing intracellular calcium levels.
Acetylcholine ; Animals ; Antidepressive Agents ; Caffeine ; Calcimycin ; Calcium* ; Cats ; Endoplasmic Reticulum ; Mouth ; Ryanodine ; Salivation*

No abstract available.

No abstract available.
Adenocarcinoma* ; Colon* ; DNA* ; Flow Cytometry* ; Ploidies*

OBJECTIVE: Uterine cervical cancer is the most common malignant tumor in Korean women. Human papillomaviruses are associated in 85-90% of the cases. However, other cofactors are considered to be important in carcinogenesis. There is an evidence that the uterine cervix is the site of shedding of the Epstein-Barr viruses(EBV). Furthermore the virus has been detected in cervical intraepithelial neoplasia and invasive carcinoma of the uterine cervix. We studied to evaluate the role of EBV in cervical carcinogenesis. METHODS: Non-neoplastic cervices(12), carcinoma in situ(32), microinvasive squamous cell carcinomas(9), invasive squamous cell carcinomas(37) and adenocarcinomas and adenosquamous carcinomas(14) were studied for EBV infection. PCR and in situ PCR for EBNA-1 were done and subtyping was done using PCR for EBNA 3C. RESULTS: In non-neoplastic cervix, EBV was detected in 16.7% by PCR and found in normal epithelial cells and lymphocytes in in situ PCR. By PCR technique, EBV was detected in 65.6% of CIS, 66.3% and 51.4% of microinvasive and invasive squamous cell carcinomas, 57.1% of adenocarcinomas and adenosquamous carcinomas. EBV subtyping was done in EBV positive cases by PCR and all showed type 1. CONCLUSION: EBV was detected in higher frequency in cervical cancer than in non-neoplastic cervix. However the frequency was not correlated to the invasion depth and histologic types of cervical carcinomas. EBV was detected in tumor cells as well as normal epithelial cells and lymphocytes also. It was suggested that EBV may play a role in cervical cancers but the mechanism in carcinogenesis remains to be solved.
Adenocarcinoma ; Carcinogenesis ; Carcinoma, Adenosquamous ; Carcinoma, Squamous Cell ; Cervical Intraepithelial Neoplasia ; Cervix Uteri ; Epithelial Cells ; Epstein-Barr Virus Infections ; Female ; Herpesvirus 4, Human* ; Humans ; Lymphocytes ; Polymerase Chain Reaction* ; Prevalence* ; Uterine Cervical Neoplasms*

Authors performed 25 corneal grafts in 24 patients since the inauguration of The Central Eye Bank, attached to the Department of Ophthalmology, St. Mary's Hospital, Catholic Medical College, in April 19, 1967. Method and results of these cases are as follows; METHOD: In technique of surgery, the grafts in most of the cases were 7 mm in diameter(Table 3), in two types of penetrating and lamellar corneal grafts(Table 4). The donor material came from patient age group of 51-60 years old in most and was used within 24 hours after death (Table 5). In most cases we placed 12 interrupted sutures except of two cases for continuous suture by 8-0 virgin silks. Preoperative and postoperative cares were routine with systemic dexamethasone, 7.0mg a day was given routinely on the 5 th postoperative day for 30-50 days because of prevention of graft rejection. RESULTS AND SUMMARY: Table 7 summbrizes the results of our observation. Nineteen of the 25 grafts remained clear. And there was improved vision above 20/200 in ten eyes out of nineteen clear grafts. Table 10 lists the reasons why nine clear grafts did not improve vision. Postoperative complications occurred in fourteen grafts (Table 11). Penetrating corneal grafts for adherent leucoma courneae, staphyloma corneae and corneal scars from alkaline burn were failed to maintaining the clear graft. The donor material, the recipient cornea, graft rejection as a complication and its prevantion were discussed. ACKNOWLEDGEMENTS: We wish to express our sincere gratitude to an those who have guided in carrying out the present investigation. In particular, We are jndebted to Dr. Bon Sool Koo, Former Chief and Professor, Department of Ophthalmology, Catholic Medical College. Dr. Sang Wook Rhee. Chief and Associate Professor, Department of Ophthalmology, Catholic Medical College.
Burns ; Cicatrix ; Cornea ; Corneal Transplantation* ; Dexamethasone ; Eye Banks ; Graft Rejection ; Humans ; Ophthalmology ; Postoperative Complications ; Silk ; Sutures ; Tissue Donors ; Transplants*

BACKGROUND: From 1980s the methamphetamine(phillopon) was suddenly spread to our country and it becomes a serious social problem. Although the proportion of transfusion associated hepatitis C declined after introduction of Anti-HCV assay as screening test of blood donors, the overall prevalence of hepatitis C has remained the same, primarily because of increase of the proportion of hepatitis C patients among intravenous drug abusers. So we studied about prevalence of Anti-HCV, and other viral infectious disease transmission by intravenous drug abuse. METHODS: Total 202 drug abusers who had been admitted to Taegu Medical Center in 1994-1996 were tested for the presence of Anti-HCV. Epidemiologic data and histories related to drug use were obtained, as were serum test for hepatitis B, aminotransferase, HIV. RESULTS: The overall seropositivity of anti-HCV was 79.2%. Patients In HCV positive group were significant old age(34.0 yrs) and had long duration of injection(3.0 yrs) and the number of patient who had elevated aminotransferase level were significantly more than HCV negative group(p<0.05). No significant difference in sex, prevalence of HBs Ag and Anti HBc were observated between HCV positive group and negative group. The prevalence of Anti HBc was 52.1% and HBs Ag was 4.2%, but anyone was not detected for AIDS. CONCLUSIONS: The seropositivity of Anti-HCV in intravenous drug users in Taegu, Korea was 79.2%, comparable to that in Europe and U.S.A. This finding suggest that its time to hurry up for medical and public walfare service personal to concern and education of drug abusers, and inview of preventive medical care the need of general public information and education is pressing.
Blood Donors ; Daegu ; Disease Transmission, Infectious ; Drug Users* ; Education ; Europe ; Hepatitis B ; Hepatitis C ; HIV ; Humans ; Korea ; Mass Screening ; Prevalence ; Social Problems ; Substance Abuse, Intravenous

No abstract available.
Appendicitis* ; Child* ; Humans