1.An Experimental Study about the Effects of Parathyroid Hormone on Osteoblasts
Seung Woo SUH ; Seok Hyun LEE ; Jae Seok CHANG ; Myong Kon KIM
The Journal of the Korean Orthopaedic Association 1996;31(1):140-153
Authors concluded an experimental study in order to find out what the effects of parathyroid hormone on cells derived from human Trabecular bone in vitro are on terms of proliferation of cells, adenylate cyclase activity and production of small substances such as osteocalcin and collagen type I. The results were as follows; 1. 3H-thymidine incorporation into cultured osteoblast was initially low (day 1–2), increased by exponential curve from day 3 till day 11. The uptakes of 3H-thymidine by osteoblasts, when bovine parathyroid hormone was added, increased dose-dependently. Greater increments were seen at concentrations of bovine PTH higher than 5.0 X10-8 mole. 2. Intracellular c-AMP accumulation was stimulated by bovine PTH in a dose-dependent manner and greater production was seen at concentrations of bovine PHT higher than 5.0 X10-8 mole. Maximal stimulation was observed at 1 X10-7 mole of bovine PTH. The concentrations of c-AMP were observed significantly higher in the presence of bovine PTH in a dose-dependent manner when compared to those of bovine PTH-absent culture condition. 3. Treatment with bovine parathyroid hormone of cultured osteoblasts resulted in increase of alkaline phophatase activity and synthesis of osteocalcin in the cultured media in a dose-dependent manner. Greater productions were observed at concentrations of bovine PTH higher than 5.0 X10-8 mole. 4. Synthesis of type I collagen by cultured osteoblasts in bovine PTH-added media was inversely proportional to their concentration, whereas control group showed minimal increments of no significance. These in vitro findings may suggest that PTH has a stimulatory effect on proliferation of osteoblast, c-AMP production and alkaline phosphatase activity in a direct manner.
Adenylyl Cyclases
;
Alkaline Phosphatase
;
Cell Proliferation
;
Collagen Type I
;
Humans
;
In Vitro Techniques
;
Osteoblasts
;
Osteocalcin
;
Parathyroid Hormone
2.Two cases of clear cell syringoma.
Seok Jong LEE ; Ho Chull SUH ; Jae Bok JUN ; Sang Lip CHUNG
Korean Journal of Dermatology 1993;31(6):1003-1008
Two cases of clear cell syringoma are reported in the women who were 39 ad 54 years old, respectively. They had had asymptomatic, ricegrain-sized skin colored to yellowish pink papules on the face, particularly on he lower eyelid for several years. Interestingly, both of them had suffered from diabetes mellitus for more than a decade and been placed on irregular antidiabetic medications. Routine laboratory findings in the elder patient(case 2), unfortunately not checked in the younger patient(cases 1) due to her refusal, were within normal limits except marked glucosuria. Histologic examination revealed numerous various-sized, well-defined tumor nests composed largely of clear cells with polygonal cellular boundary and eccentric nucleus. Special stain with Periodic acid-Schiff disclosed existance of glycogen in the clear cells, but other special stains such as alcian blue showed negative findings. Electron microscopic examination of the elder patient revealed many multivesicular bodies suggestive of lysosomes, and numerous droplets of glycogen dispersed between them in villi-rich periluminal cell.
Alcian Blue
;
Coloring Agents
;
Diabetes Mellitus
;
Disulfiram
;
Eyelids
;
Female
;
Glycogen
;
Humans
;
Lysosomes
;
Middle Aged
;
Multivesicular Bodies
;
Skin
;
Syringoma*
3.Living-donor renal transplantation, univariate analysis of risk factors influencing renal allograft outcome from 500 cases.
Kiil PARK ; Jae Seok SUH ; Yu Seun KIM ; Soon Il KIM
Journal of the Korean Surgical Society 1991;41(5):616-627
No abstract available.
Allografts*
;
Kidney Transplantation*
;
Risk Factors*
4.Changes of Serum Inhibin Concentration in Controlled Ovarian Hyperstimulation for IVF-ET.
Shin Yong MOON ; Seok Hyun KIM ; Jae Hoon LEE ; Chang Suk SUH
Korean Journal of Obstetrics and Gynecology 1998;41(11):2795-2805
Inhibin is a glycoprotein usually produced by granulosa cell of ovary and is known as regulator of FSH secretion. Inhibin is consisted of two heterodimeric subunit of a, B . There are two distinct molecular forms of the B subunit that exist (BA and BB) and when combined with an a subunit, form inhibin A, and inhibin B, respectively. Ovarian reserve describes women's reproductive potential, which usually decreased according to chronological age. Because useful markers claimed for the prediction of ovarian response during controlled ovarian hyperstimulation for IVF-ET have not been always successful, new biomarker has been investigated. The purpose of this study was to observe the changes in serum and ovarian follicular inhibin A concentration during controlled ovarian hyperstimulation for IVF-ET program and to evaluate the clinical significance of inhibin A as a prognostic marker for assisted reproductive technology outcomes. From Jan 1996 to Dec 1996, 48 patients who underwent IVF-ET were included. In each patient, the Day 3 FSH, LH, E2q were measured in the first month of cycle before commencing GnRH agonist administration, In the stimulation period after pituitary down regulation, blood samples for inhibin A were collected at the day 3, day 7, day of hCG injection, day of oocytes aspiration, and day of embryo transfer. Ovarian follicular inhibin A was collected at the day of oocyte aspiration. Inhibin A concentration was measured using test kit for inhibin A (INHIBIN-EASIA (code 40.134.00), Belgium Medgenix). The changes in serum inhibin A, E2 concentration during IVF-ET program showed increasing pattern throughout controlled ovarian hyperstimulation periods. If the cut-off value of day 3 serum inhibin A determined to 0.28 U/ml, the sensitivity and specificity were 80.0%, 75.0% respectively in predicting the number of oocytes retrieved being more than five. If the cut-off value of peak serum E determined to 1316 pg/ml, the sensitivity and specificity were 89.0%, 100.0% respectively in predicting the number of oocytes retrieved being more than five. In conclusion, measurement of serum inhibin A concentration on the day of starting stimulation (day 3) could be used as a useful marker for predicting the ovarian response in IVF-ET program.
Belgium
;
Down-Regulation
;
Embryo Transfer
;
Female
;
Glycoproteins
;
Gonadotropin-Releasing Hormone
;
Granulosa Cells
;
Humans
;
Inhibins*
;
Oocyte Retrieval
;
Oocytes
;
Ovary
;
Reproductive Techniques, Assisted
;
Sensitivity and Specificity
;
Serum
5.Predictive value of serum beta-hCG level in pregnancies following in vitro fertilization and embryo transfer.
Seok Hyun KIM ; Chang Suk SUH ; Doo Seok CHOI ; Young Min CHOI ; Chang Jae SHIN ; Jung Gu KIM ; Shin Yong MOON ; Jin Yong LEE ; Yoon Seok CHANG
Korean Journal of Fertility and Sterility 1992;19(1):41-48
No abstract available.
Embryo Transfer*
;
Embryonic Structures*
;
Fertilization in Vitro*
;
Pregnancy*
6.Natural Evolution of Grafted Anterior Cruciate Ligament of the Knee: Prospective Follow-up MR Studies.
Dong Won PARK ; Jae Hyun CHO ; Yon Kwon IHN ; Yong Woon SHIM ; Jin Seok SUH ; Byung Heum MIN ; Jung Ho SUH
Journal of the Korean Radiological Society 1998;38(1):151-157
PURPOSE: To described the MR findings in the periodic changes of the size and signal intensity ofreconstructed anterior cruciate ligament (ACL) of the knee and the efficacy of oblique axial imaging in patientswho underwent arthroscopic ACL reconstruction using autogenous patellar tendon. MATERIALS AND METHODS: Twenty-onepatients who had undergone ACL reconstruction were evaluated by follow-up MRI at postoperative 1 week, 1, 2, 3 and6 months, and 1 year, Conventional Fast-Spin-Echo (FSE) sagittal and coronal images and oblique axial images(proton density and T2-WI; VEMP TR/TE, 2000/20/70) were obtained perpendicular to the ligament, and using an ROIcurve in the intra-articular area, periodic changes in the cross-sectional area were evaluated by proton densityimaging and signal intensity by T2WI imaging. Cross-sectional morphology was categorized as either smooth, andround or notch-shaped and periodic changes in configuration were observed. In three cases, we evaluated the retearof reconstructed ACL by comparing the oblique axizl image obtained by conventional sagittal and coronal imaging. RESULTS: The cross-sectional area and signal intensity of grafted ACL increased significantly (p<0.05) after 3months and at 1 year, respectively. Cross-sectional morphology was smooth and round in 86% of cases, and notchedin 14%, and during follow-up MR studies, no periodic changes were seen. Notch-shaped ACL and decreased perigraftsignal intensity, as seen on sagittal images, could lead to a misdiagnosis of partial tear ; on oblique axialimages, ACL and perigraft signal intensity were found to be normal. CONCLUSION: During the natural evolution ofgrafted ACL, cross-sectional area and signal intensity increased significantly after 3 months and at 1 year,respectively ; on follow-up MR studies, cross-sectional morphology did not change, however. Oblique axial imagingprovides important information for evaluating whether the reconstructed ACL is torn or not.
Anterior Cruciate Ligament*
;
Cross-Sectional Studies
;
Diagnostic Errors
;
Follow-Up Studies*
;
Knee*
;
Ligaments
;
Magnetic Resonance Imaging
;
Patellar Ligament
;
Prospective Studies*
;
Protons
;
Transplants*
7.Impact of early graft function on the long-term outcome of living donor kidney transplant(TLX).
Yu Seun KIM ; Jae Seok SUH ; Soon Il KIM ; Ik Yong KIM ; Sung Sang MOON ; Kiil PARK
The Journal of the Korean Society for Transplantation 1991;5(1):109-111
No abstract available.
Humans
;
Kidney*
;
Living Donors*
;
Transplants*
8.Pelvis dilatation and mucosal thickening of transplanted kidney: comparative study of resistive index and ultrasonographic finding.
Myung Joon KIM ; Hyung Sik YOO ; Yu Seun KIM ; Jae Seok SUH ; Sung Sang MOON ; Jong Tae LEE ; Kiil PARK
The Journal of the Korean Society for Transplantation 1991;5(1):101-107
No abstract available.
Dilatation*
;
Kidney*
;
Pelvis*
9.Autoimmune hemolytic anemia after renal transplantation.
Soon Il KIM ; Jae Seok SUH ; Hyun Ok KIM ; Ik Yong KIM ; Yu Seun KIM ; Kiil PARK
The Journal of the Korean Society for Transplantation 1991;5(1):91-94
No abstract available.
Anemia, Hemolytic, Autoimmune*
;
Kidney Transplantation*
10.Renal transplantation is not contraindicated in hepatitis B surface antigen positive patients.
Jae Seok SUH ; Yu Seun KIM ; Soon Il KIM ; Yong Suk YANG ; Chae Yoon CHON ; Kiil PARK
The Journal of the Korean Society for Transplantation 1991;5(1):63-67
No abstract available.
Hepatitis B Surface Antigens*
;
Hepatitis B*
;
Hepatitis*
;
Humans
;
Kidney Transplantation*