To understamd the changes in parvalbumm- and calbindin-immunoreactive neurons and fibers during the spinal cord ischemia, expression of two kinds of calcium bindlng protein, parvalbumin (PV) and calbindin D-28K (calbindin), immunocytochemically an activities of cytochrome oxidase (CO) and acetylcholinesterase (AChE), histochemically global ischemic spinal cord of the rabbit were investigated under thiopental sodium anesthesia, adult rabbits(2,0-2.5 Kg) was cannulated through the left femoral artery with 4F Berman amgiographic catheter. Which was advanced 15 so that tbe tip of the catheter was in the ahdominal aorta just below the orign of the renal artery. After the rabbits were recovered from anesthesia, the balloon was inflated induce ischemia for15 minutes with recording abdominal aortic blood pressure. At 48 hours after reperfusion, the rabbits were fixed with transcardiac perfusion. In the sham-operated controls, the rabbit was treated same procedure except inflation of the ball. In tbe ischemic spinal cord, CO activitieS were generally decreased in the gray matter, especially in laminae III-V and AChE activities were slightly decreased in laminae X amd III-IV. The degree of immunoreactivities for PV and calbindin was decreased in whole gray matter of ischemic cord. In laminae II-III of the ischemic lumbar cord, number of PV amd calbindin-immunoreactive neurons was signilicantly decreased and vacuolations were observed. In laminae V-IX amd X of ischemic lumbar cord. The numbe of jmmunoreactive neurons was not changed, but degree of immunoreactivities for and calbindin was decreased. Thses findings suggest that PV and calbindin-immunoreactive neurons in laminae II-III are more vulnerable than other neurons to ischemic injury, and PV and calbindin may have some roles in hypoxic neuronal injury.
Acetylcholinesterase ; Adult ; Anesthesia ; Aorta ; Arterial Pressure ; Calbindins ; Calcium ; Catheters ; Electron Transport Complex IV ; Femoral Artery ; Humans ; Inflation, Economic ; Ischemia* ; Neurons ; Perfusion ; Rabbits ; Renal Artery ; Reperfusion ; Spinal Cord Ischemia ; Spinal Cord* ; Thiopental